BuceA.00121.a

Biotin synthase

CENTER ID: BuceA.00121.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00121.a.B1.GE43978 Full length(BuceA.00121.a) 1 339
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00121.a.B1.PS38140 Full length(BuceA.00121.a) 1 339
BuceA.00121.a.B1.PW38922 Full length(BuceA.00121.a) 1 339
BuceA.00121.a.B1.PW38923 Full length(BuceA.00121.a) 1 339

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.695
UniProt: B4E9L4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQAQTVAVQ SDAIPMAAPA PQRWRVADVV ALFELPFNDL MFRAQQVHRE HFDANAVQLS TLLSIKTGGC EEDCGYCSQS SHHDTGLKAE KLMDVDTVLD AARAAKANGA SRFCMGAAWR NPKERHMPAL TEMVRGVKEL GLETCMTLGM LEDEQARQLA DAGLDYYNHN LDTSPEFYGQ VISTRTYQDR LDTLDRVRDA GINVCCGGII GMGESRRERA GLISQLANLN PYPESVPINN LVAIEGTPLE GTAPLDPFEF VRTIAVARIT MPKAVVRLSA GREQLDDAMQ AMCFLAGANS MFYGDQLLTT SNPQTQRDRA LFERLGIRAS QADALSDNA
NT Sequence
ATGACCCAAG CCCAGACCGT CGCCGTGCAA TCCGACGCGA TTCCCATGGC TGCACCGGCG CCGCAGCGCT GGCGCGTCGC CGACGTCGTC GCGCTGTTCG AACTGCCGTT CAACGACCTG ATGTTCCGCG CGCAGCAGGT GCATCGCGAG CACTTCGACG CGAACGCGGT GCAGCTGTCG ACGCTGCTGT CGATCAAGAC GGGCGGCTGC GAGGAAGATT GCGGGTACTG CTCGCAGTCG TCGCATCACG ACACGGGCCT GAAGGCCGAG AAGCTGATGG ACGTCGACAC GGTGCTCGAC GCCGCGCGCG CGGCGAAGGC GAACGGCGCG AGCCGCTTCT GCATGGGCGC CGCGTGGCGC AACCCGAAGG AGCGCCACAT GCCGGCGCTG ACCGAGATGG TGCGCGGCGT GAAGGAGCTC GGCCTCGAGA CCTGCATGAC GCTCGGCATG CTCGAAGACG AGCAGGCTCG GCAGCTCGCC GACGCGGGCC TCGACTACTA CAACCACAAC CTCGACACGT CGCCGGAGTT CTACGGCCAG GTGATCTCGA CGCGCACCTA CCAGGATCGC CTCGACACAC TCGACCGCGT GCGCGACGCG GGCATCAACG TGTGCTGCGG CGGCATCATC GGGATGGGCG AATCGCGTCG CGAACGCGCG GGCCTGATCT CGCAGCTCGC GAACCTGAAC CCGTATCCGG AATCGGTGCC GATCAACAAC CTCGTCGCGA TCGAAGGCAC GCCGCTCGAA GGCACCGCGC CGCTCGATCC GTTCGAATTC GTGCGCACCA TCGCGGTCGC GCGCATCACG ATGCCGAAGG CCGTCGTGCG CCTGTCGGCC GGGCGCGAGC AGCTCGACGA CGCGATGCAG GCGATGTGCT TCCTCGCCGG TGCGAACTCG ATGTTCTACG GCGACCAGCT GCTGACGACG AGCAACCCGC AGACGCAGCG CGACCGCGCG CTGTTCGAGC GCCTCGGCAT CCGCGCGAGC CAGGCCGACG CGCTGTCGGA CAACGCG
Details for BuceA.00121.a.B1.GE43978
HARVESTED ON: 12/3/2020
SEQUENCED ON: 12/16/2020
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMT QAQTVAVQSD AIPMAAPAPQ RWRVADVVAL FELPFNDLMF RAQQVHREHF DANAVQLSTL LSIKTGGCEE DCGYCSQSSH HDTGLKAEKL MDVDTVLDAA RAAKANGASR FCMGAAWRNP KERHMPALTE MVRGVKELGL ETCMTLGMLE DEQARQLADA GLDYYNHNLD TSPEFYGQVI STRTYQDRLD TLDRVRDAGI NVCCGGIIGM GESRRERAGL ISQLANLNPY PESVPINNLV AIEGTPLEGT APLDPFEFVR TIAVARITMP KAVVRLSAGR EQLDDAMQAM CFLAGANSMF YGDQLLTTSN PQTQRDRALF ERLGIRASQA
Validated NT Sequence
atggctcacc accaccacca ccatatgacc caagcccaga ccgtcgccgt gcaatccgac gcgattccca tggctgcacc ggcgccgcag cgctggcgcg tcgccgacgt cgtcgcgctg ttcgaactgc cgttcaacga cctgatgttc cgcgcgcagc aggtgcatcg cgagcacttc gacgcgaacg cggtgcagct gtcgacgctg ctgtcgatca agacgggcgg ctgcgaggaa gattgcgggt actgctcgca gtcgtcgcat cacgacacgg gcctgaaggc cgagaagctg atggacgtcg acacggtgct cgacgccgcg cgcgcggcga aggcgaacgg cgcgagccgc ttctgcatgg gcgccgcgtg gcgcaacccg aaggagcgcc acatgccggc gctgaccgag atggtgcgcg gcgtgaagga gctcggcctc gagacctgca tgacgctcgg catgctcgaa gacgagcagg ctcggcagct cgccgacgcg ggcctcgact actacaacca caacctcgac acgtcgccgg agttctacgg ccaggtgatc tcgacgcgca cctaccagga tcgcctcgac acactcgacc gcgtgcgcga cgcgggcatc aacgtgtgct gcggcggcat catcgggatg ggcgaatcgc gtcgcgaacg cgcgggcctg atctcgcagc tcgcgaacct gaacccgtat ccggaatcgg tgccgatcaa caacctcgtc gcgatcgaag gcacgccgct cgaaggcacc gcgccgctcg atccgttcga attcgtgcgc accatcgcgg tcgcgcgcat cacgatgccg aaggccgtcg tgcgcctgtc ggccgggcgc gagcagctcg acgacgcgat gcaggcgatg tgcttcctcg ccggtgcgaa ctcgatgttc tacggcgacc agctgctgac gacgagcaac ccgcagacgc agcgcgaccg cgcgctgttc gagcgcctcg gcatccgcgc gagccaggcc gac
Expected Protein Sequence
MAHHHHHHMT QAQTVAVQSD AIPMAAPAPQ RWRVADVVAL FELPFNDLMF RAQQVHREHF DANAVQLSTL LSIKTGGCEE DCGYCSQSSH HDTGLKAEKL MDVDTVLDAA RAAKANGASR FCMGAAWRNP KERHMPALTE MVRGVKELGL ETCMTLGMLE DEQARQLADA GLDYYNHNLD TSPEFYGQVI STRTYQDRLD TLDRVRDAGI NVCCGGIIGM GESRRERAGL ISQLANLNPY PESVPINNLV AIEGTPLEGT APLDPFEFVR TIAVARITMP KAVVRLSAGR EQLDDAMQAM CFLAGANSMF YGDQLLTTSN PQTQRDRALF ERLGIRASQA DALSDNA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc aagcccagac cgtcgccgtg caatccgacg cgattcccat ggctgcaccg gcgccgcagc gctggcgcgt cgccgacgtc gtcgcgctgt tcgaactgcc gttcaacgac ctgatgttcc gcgcgcagca ggtgcatcgc gagcacttcg acgcgaacgc ggtgcagctg tcgacgctgc tgtcgatcaa gacgggcggc tgcgaggaag attgcgggta ctgctcgcag tcgtcgcatc acgacacggg cctgaaggcc gagaagctga tggacgtcga cacggtgctc gacgccgcgc gcgcggcgaa ggcgaacggc gcgagccgct tctgcatggg cgccgcgtgg cgcaacccga aggagcgcca catgccggcg ctgaccgaga tggtgcgcgg cgtgaaggag ctcggcctcg agacctgcat gacgctcggc atgctcgaag acgagcaggc tcggcagctc gccgacgcgg gcctcgacta ctacaaccac aacctcgaca cgtcgccgga gttctacggc caggtgatct cgacgcgcac ctaccaggat cgcctcgaca cactcgaccg cgtgcgcgac gcgggcatca acgtgtgctg cggcggcatc atcgggatgg gcgaatcgcg tcgcgaacgc gcgggcctga tctcgcagct cgcgaacctg aacccgtatc cggaatcggt gccgatcaac aacctcgtcg cgatcgaagg cacgccgctc gaaggcaccg cgccgctcga tccgttcgaa ttcgtgcgca ccatcgcggt cgcgcgcatc acgatgccga aggccgtcgt gcgcctgtcg gccgggcgcg agcagctcga cgacgcgatg caggcgatgt gcttcctcgc cggtgcgaac tcgatgttct acggcgacca gctgctgacg acgagcaacc cgcagacgca gcgcgaccgc gcgctgttcg agcgcctcgg catccgcgcg agccaggccg acgcgctgtc ggacaacgcg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuceA.00121.a.B1.PS38140
PURIFICATION DATe: 12/7/2016
CONCENTRATION: 33.1mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT QAQTVAVQSD AIPMAAPAPQ RWRVADVVAL FELPFNDLMF RAQQVHREHF DANAVQLSTL LSIKTGGCEE DCGYCSQSSH HDTGLKAEKL MDVDTVLDAA RAAKANGASR FCMGAAWRNP KERHMPALTE MVRGVKELGL ETCMTLGMLE DEQARQLADA GLDYYNHNLD TSPEFYGQVI STRTYQDRLD TLDRVRDAGI NVCCGGIIGM GESRRERAGL ISQLANLNPY PESVPINNLV AIEGTPLEGT APLDPFEFVR TIAVARITMP KAVVRLSAGR EQLDDAMQAM CFLAGANSMF YGDQLLTTSN PQTQRDRALF ERLGIRASQA
Validated NT Sequence
atggctcacc accaccacca ccatatgacc caagcccaga ccgtcgccgt gcaatccgac gcgattccca tggctgcacc ggcgccgcag cgctggcgcg tcgccgacgt cgtcgcgctg ttcgaactgc cgttcaacga cctgatgttc cgcgcgcagc aggtgcatcg cgagcacttc gacgcgaacg cggtgcagct gtcgacgctg ctgtcgatca agacgggcgg ctgcgaggaa gattgcgggt actgctcgca gtcgtcgcat cacgacacgg gcctgaaggc cgagaagctg atggacgtcg acacggtgct cgacgccgcg cgcgcggcga aggcgaacgg cgcgagccgc ttctgcatgg gcgccgcgtg gcgcaacccg aaggagcgcc acatgccggc gctgaccgag atggtgcgcg gcgtgaagga gctcggcctc gagacctgca tgacgctcgg catgctcgaa gacgagcagg ctcggcagct cgccgacgcg ggcctcgact actacaacca caacctcgac acgtcgccgg agttctacgg ccaggtgatc tcgacgcgca cctaccagga tcgcctcgac acactcgacc gcgtgcgcga cgcgggcatc aacgtgtgct gcggcggcat catcgggatg ggcgaatcgc gtcgcgaacg cgcgggcctg atctcgcagc tcgcgaacct gaacccgtat ccggaatcgg tgccgatcaa caacctcgtc gcgatcgaag gcacgccgct cgaaggcacc gcgccgctcg atccgttcga attcgtgcgc accatcgcgg tcgcgcgcat cacgatgccg aaggccgtcg tgcgcctgtc ggccgggcgc gagcagctcg acgacgcgat gcaggcgatg tgcttcctcg ccggtgcgaa ctcgatgttc tacggcgacc agctgctgac gacgagcaac ccgcagacgc agcgcgaccg cgcgctgttc gagcgcctcg gcatccgcgc gagccaggcc gac
Expressed Protein Sequence
MAHHHHHHMT QAQTVAVQSD AIPMAAPAPQ RWRVADVVAL FELPFNDLMF RAQQVHREHF DANAVQLSTL LSIKTGGCEE DCGYCSQSSH HDTGLKAEKL MDVDTVLDAA RAAKANGASR FCMGAAWRNP KERHMPALTE MVRGVKELGL ETCMTLGMLE DEQARQLADA GLDYYNHNLD TSPEFYGQVI STRTYQDRLD TLDRVRDAGI NVCCGGIIGM GESRRERAGL ISQLANLNPY PESVPINNLV AIEGTPLEGT APLDPFEFVR TIAVARITMP KAVVRLSAGR EQLDDAMQAM CFLAGANSMF YGDQLLTTSN PQTQRDRALF ERLGIRASQA DALSDNA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC AAGCCCAGAC CGTCGCCGTG CAATCCGACG CGATTCCCAT GGCTGCACCG GCGCCGCAGC GCTGGCGCGT CGCCGACGTC GTCGCGCTGT TCGAACTGCC GTTCAACGAC CTGATGTTCC GCGCGCAGCA GGTGCATCGC GAGCACTTCG ACGCGAACGC GGTGCAGCTG TCGACGCTGC TGTCGATCAA GACGGGCGGC TGCGAGGAAG ATTGCGGGTA CTGCTCGCAG TCGTCGCATC ACGACACGGG CCTGAAGGCC GAGAAGCTGA TGGACGTCGA CACGGTGCTC GACGCCGCGC GCGCGGCGAA GGCGAACGGC GCGAGCCGCT TCTGCATGGG CGCCGCGTGG CGCAACCCGA AGGAGCGCCA CATGCCGGCG CTGACCGAGA TGGTGCGCGG CGTGAAGGAG CTCGGCCTCG AGACCTGCAT GACGCTCGGC ATGCTCGAAG ACGAGCAGGC TCGGCAGCTC GCCGACGCGG GCCTCGACTA CTACAACCAC AACCTCGACA CGTCGCCGGA GTTCTACGGC CAGGTGATCT CGACGCGCAC CTACCAGGAT CGCCTCGACA CACTCGACCG CGTGCGCGAC GCGGGCATCA ACGTGTGCTG CGGCGGCATC ATCGGGATGG GCGAATCGCG TCGCGAACGC GCGGGCCTGA TCTCGCAGCT CGCGAACCTG AACCCGTATC CGGAATCGGT GCCGATCAAC AACCTCGTCG CGATCGAAGG CACGCCGCTC GAAGGCACCG CGCCGCTCGA TCCGTTCGAA TTCGTGCGCA CCATCGCGGT CGCGCGCATC ACGATGCCGA AGGCCGTCGT GCGCCTGTCG GCCGGGCGCG AGCAGCTCGA CGACGCGATG CAGGCGATGT GCTTCCTCGC CGGTGCGAAC TCGATGTTCT ACGGCGACCA GCTGCTGACG ACGAGCAACC CGCAGACGCA GCGCGACCGC GCGCTGTTCG AGCGCCTCGG CATCCGCGCG AGCCAGGCCG ACGCGCTGTC GGACAACGCG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for BuceA.00121.a.B1.PW38922
PURIFICATION DATe: 1/11/2021
CONCENTRATION: 10mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT QAQTVAVQSD AIPMAAPAPQ RWRVADVVAL FELPFNDLMF RAQQVHREHF DANAVQLSTL LSIKTGGCEE DCGYCSQSSH HDTGLKAEKL MDVDTVLDAA RAAKANGASR FCMGAAWRNP KERHMPALTE MVRGVKELGL ETCMTLGMLE DEQARQLADA GLDYYNHNLD TSPEFYGQVI STRTYQDRLD TLDRVRDAGI NVCCGGIIGM GESRRERAGL ISQLANLNPY PESVPINNLV AIEGTPLEGT APLDPFEFVR TIAVARITMP KAVVRLSAGR EQLDDAMQAM CFLAGANSMF YGDQLLTTSN PQTQRDRALF ERLGIRASQA
Validated NT Sequence
atggctcacc accaccacca ccatatgacc caagcccaga ccgtcgccgt gcaatccgac gcgattccca tggctgcacc ggcgccgcag cgctggcgcg tcgccgacgt cgtcgcgctg ttcgaactgc cgttcaacga cctgatgttc cgcgcgcagc aggtgcatcg cgagcacttc gacgcgaacg cggtgcagct gtcgacgctg ctgtcgatca agacgggcgg ctgcgaggaa gattgcgggt actgctcgca gtcgtcgcat cacgacacgg gcctgaaggc cgagaagctg atggacgtcg acacggtgct cgacgccgcg cgcgcggcga aggcgaacgg cgcgagccgc ttctgcatgg gcgccgcgtg gcgcaacccg aaggagcgcc acatgccggc gctgaccgag atggtgcgcg gcgtgaagga gctcggcctc gagacctgca tgacgctcgg catgctcgaa gacgagcagg ctcggcagct cgccgacgcg ggcctcgact actacaacca caacctcgac acgtcgccgg agttctacgg ccaggtgatc tcgacgcgca cctaccagga tcgcctcgac acactcgacc gcgtgcgcga cgcgggcatc aacgtgtgct gcggcggcat catcgggatg ggcgaatcgc gtcgcgaacg cgcgggcctg atctcgcagc tcgcgaacct gaacccgtat ccggaatcgg tgccgatcaa caacctcgtc gcgatcgaag gcacgccgct cgaaggcacc gcgccgctcg atccgttcga attcgtgcgc accatcgcgg tcgcgcgcat cacgatgccg aaggccgtcg tgcgcctgtc ggccgggcgc gagcagctcg acgacgcgat gcaggcgatg tgcttcctcg ccggtgcgaa ctcgatgttc tacggcgacc agctgctgac gacgagcaac ccgcagacgc agcgcgaccg cgcgctgttc gagcgcctcg gcatccgcgc gagccaggcc gac
Expressed Protein Sequence
MAHHHHHHMT QAQTVAVQSD AIPMAAPAPQ RWRVADVVAL FELPFNDLMF RAQQVHREHF DANAVQLSTL LSIKTGGCEE DCGYCSQSSH HDTGLKAEKL MDVDTVLDAA RAAKANGASR FCMGAAWRNP KERHMPALTE MVRGVKELGL ETCMTLGMLE DEQARQLADA GLDYYNHNLD TSPEFYGQVI STRTYQDRLD TLDRVRDAGI NVCCGGIIGM GESRRERAGL ISQLANLNPY PESVPINNLV AIEGTPLEGT APLDPFEFVR TIAVARITMP KAVVRLSAGR EQLDDAMQAM CFLAGANSMF YGDQLLTTSN PQTQRDRALF ERLGIRASQA DALSDNA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC AAGCCCAGAC CGTCGCCGTG CAATCCGACG CGATTCCCAT GGCTGCACCG GCGCCGCAGC GCTGGCGCGT CGCCGACGTC GTCGCGCTGT TCGAACTGCC GTTCAACGAC CTGATGTTCC GCGCGCAGCA GGTGCATCGC GAGCACTTCG ACGCGAACGC GGTGCAGCTG TCGACGCTGC TGTCGATCAA GACGGGCGGC TGCGAGGAAG ATTGCGGGTA CTGCTCGCAG TCGTCGCATC ACGACACGGG CCTGAAGGCC GAGAAGCTGA TGGACGTCGA CACGGTGCTC GACGCCGCGC GCGCGGCGAA GGCGAACGGC GCGAGCCGCT TCTGCATGGG CGCCGCGTGG CGCAACCCGA AGGAGCGCCA CATGCCGGCG CTGACCGAGA TGGTGCGCGG CGTGAAGGAG CTCGGCCTCG AGACCTGCAT GACGCTCGGC ATGCTCGAAG ACGAGCAGGC TCGGCAGCTC GCCGACGCGG GCCTCGACTA CTACAACCAC AACCTCGACA CGTCGCCGGA GTTCTACGGC CAGGTGATCT CGACGCGCAC CTACCAGGAT CGCCTCGACA CACTCGACCG CGTGCGCGAC GCGGGCATCA ACGTGTGCTG CGGCGGCATC ATCGGGATGG GCGAATCGCG TCGCGAACGC GCGGGCCTGA TCTCGCAGCT CGCGAACCTG AACCCGTATC CGGAATCGGT GCCGATCAAC AACCTCGTCG CGATCGAAGG CACGCCGCTC GAAGGCACCG CGCCGCTCGA TCCGTTCGAA TTCGTGCGCA CCATCGCGGT CGCGCGCATC ACGATGCCGA AGGCCGTCGT GCGCCTGTCG GCCGGGCGCG AGCAGCTCGA CGACGCGATG CAGGCGATGT GCTTCCTCGC CGGTGCGAAC TCGATGTTCT ACGGCGACCA GCTGCTGACG ACGAGCAACC CGCAGACGCA GCGCGACCGC GCGCTGTTCG AGCGCCTCGG CATCCGCGCG AGCCAGGCCG ACGCGCTGTC GGACAACGCG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for BuceA.00121.a.B1.PW38923
PURIFICATION DATe: 1/11/2021
CONCENTRATION: 55mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT QAQTVAVQSD AIPMAAPAPQ RWRVADVVAL FELPFNDLMF RAQQVHREHF DANAVQLSTL LSIKTGGCEE DCGYCSQSSH HDTGLKAEKL MDVDTVLDAA RAAKANGASR FCMGAAWRNP KERHMPALTE MVRGVKELGL ETCMTLGMLE DEQARQLADA GLDYYNHNLD TSPEFYGQVI STRTYQDRLD TLDRVRDAGI NVCCGGIIGM GESRRERAGL ISQLANLNPY PESVPINNLV AIEGTPLEGT APLDPFEFVR TIAVARITMP KAVVRLSAGR EQLDDAMQAM CFLAGANSMF YGDQLLTTSN PQTQRDRALF ERLGIRASQA
Validated NT Sequence
atggctcacc accaccacca ccatatgacc caagcccaga ccgtcgccgt gcaatccgac gcgattccca tggctgcacc ggcgccgcag cgctggcgcg tcgccgacgt cgtcgcgctg ttcgaactgc cgttcaacga cctgatgttc cgcgcgcagc aggtgcatcg cgagcacttc gacgcgaacg cggtgcagct gtcgacgctg ctgtcgatca agacgggcgg ctgcgaggaa gattgcgggt actgctcgca gtcgtcgcat cacgacacgg gcctgaaggc cgagaagctg atggacgtcg acacggtgct cgacgccgcg cgcgcggcga aggcgaacgg cgcgagccgc ttctgcatgg gcgccgcgtg gcgcaacccg aaggagcgcc acatgccggc gctgaccgag atggtgcgcg gcgtgaagga gctcggcctc gagacctgca tgacgctcgg catgctcgaa gacgagcagg ctcggcagct cgccgacgcg ggcctcgact actacaacca caacctcgac acgtcgccgg agttctacgg ccaggtgatc tcgacgcgca cctaccagga tcgcctcgac acactcgacc gcgtgcgcga cgcgggcatc aacgtgtgct gcggcggcat catcgggatg ggcgaatcgc gtcgcgaacg cgcgggcctg atctcgcagc tcgcgaacct gaacccgtat ccggaatcgg tgccgatcaa caacctcgtc gcgatcgaag gcacgccgct cgaaggcacc gcgccgctcg atccgttcga attcgtgcgc accatcgcgg tcgcgcgcat cacgatgccg aaggccgtcg tgcgcctgtc ggccgggcgc gagcagctcg acgacgcgat gcaggcgatg tgcttcctcg ccggtgcgaa ctcgatgttc tacggcgacc agctgctgac gacgagcaac ccgcagacgc agcgcgaccg cgcgctgttc gagcgcctcg gcatccgcgc gagccaggcc gac
Expressed Protein Sequence
MAHHHHHHMT QAQTVAVQSD AIPMAAPAPQ RWRVADVVAL FELPFNDLMF RAQQVHREHF DANAVQLSTL LSIKTGGCEE DCGYCSQSSH HDTGLKAEKL MDVDTVLDAA RAAKANGASR FCMGAAWRNP KERHMPALTE MVRGVKELGL ETCMTLGMLE DEQARQLADA GLDYYNHNLD TSPEFYGQVI STRTYQDRLD TLDRVRDAGI NVCCGGIIGM GESRRERAGL ISQLANLNPY PESVPINNLV AIEGTPLEGT APLDPFEFVR TIAVARITMP KAVVRLSAGR EQLDDAMQAM CFLAGANSMF YGDQLLTTSN PQTQRDRALF ERLGIRASQA DALSDNA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC AAGCCCAGAC CGTCGCCGTG CAATCCGACG CGATTCCCAT GGCTGCACCG GCGCCGCAGC GCTGGCGCGT CGCCGACGTC GTCGCGCTGT TCGAACTGCC GTTCAACGAC CTGATGTTCC GCGCGCAGCA GGTGCATCGC GAGCACTTCG ACGCGAACGC GGTGCAGCTG TCGACGCTGC TGTCGATCAA GACGGGCGGC TGCGAGGAAG ATTGCGGGTA CTGCTCGCAG TCGTCGCATC ACGACACGGG CCTGAAGGCC GAGAAGCTGA TGGACGTCGA CACGGTGCTC GACGCCGCGC GCGCGGCGAA GGCGAACGGC GCGAGCCGCT TCTGCATGGG CGCCGCGTGG CGCAACCCGA AGGAGCGCCA CATGCCGGCG CTGACCGAGA TGGTGCGCGG CGTGAAGGAG CTCGGCCTCG AGACCTGCAT GACGCTCGGC ATGCTCGAAG ACGAGCAGGC TCGGCAGCTC GCCGACGCGG GCCTCGACTA CTACAACCAC AACCTCGACA CGTCGCCGGA GTTCTACGGC CAGGTGATCT CGACGCGCAC CTACCAGGAT CGCCTCGACA CACTCGACCG CGTGCGCGAC GCGGGCATCA ACGTGTGCTG CGGCGGCATC ATCGGGATGG GCGAATCGCG TCGCGAACGC GCGGGCCTGA TCTCGCAGCT CGCGAACCTG AACCCGTATC CGGAATCGGT GCCGATCAAC AACCTCGTCG CGATCGAAGG CACGCCGCTC GAAGGCACCG CGCCGCTCGA TCCGTTCGAA TTCGTGCGCA CCATCGCGGT CGCGCGCATC ACGATGCCGA AGGCCGTCGT GCGCCTGTCG GCCGGGCGCG AGCAGCTCGA CGACGCGATG CAGGCGATGT GCTTCCTCGC CGGTGCGAAC TCGATGTTCT ACGGCGACCA GCTGCTGACG ACGAGCAACC CGCAGACGCA GCGCGACCGC GCGCTGTTCG AGCGCCTCGG CATCCGCGCG AGCCAGGCCG ACGCGCTGTC GGACAACGCG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT