BuceA.00120.a

GTPase obg

CENTER ID: BuceA.00120.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00120.a.A1.GE32139 Full length( BuceA.00120.a ) 1 370
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00120.a.A1.PS01116 Full length( BuceA.00120.a ) 1 370

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3572
RefSeq: YP_002232542.1
UniProt: B4E5X0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGSVYAIAD RNINTLIDYR YAKKHLARNG ENGRGSDCYG KGGDDVTLRM PVGTIISDMD TGELIADLTE HDQRVMLAQG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MLKLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSKSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGV LLHLVDLAPF DESVDPVAEA TAIVGELRKY DEALYEKPRW LVLNKLDMVP EDEREARVAD FLDRFGWDGP VFEISALTGQ GCEALCYAIY DYLSEHSDAH RAAEAEDLAA DVRFRDAPPA KGGATPGDDA
NT Sequence
ATGAAGTTCA TTGACGAAGC ACGAATCGAA GTCATCGCCG GCGACGGAGG CGATGGCAGC GCGTCGATGC GCCGCGAGAA ATTCGTCCCG TTCGGCGGGC CGGACGGTGG CGACGGCGGC CGCGGCGGTA GCGTTTACGC GATCGCCGAC CGCAACATCA ACACGCTGAT CGACTACCGT TACGCGAAGA AGCACCTCGC GCGCAACGGC GAAAACGGTC GCGGCTCGGA TTGCTACGGC AAGGGCGGCG ACGATGTCAC GCTGCGCATG CCGGTCGGCA CCATCATCAG CGACATGGAC ACGGGCGAGC TGATCGCCGA CCTGACCGAG CACGACCAGC GGGTGATGCT CGCGCAGGGC GGCGCCGGCG GCCTCGGCAA CCTGCATTTC AAGTCGAGCA CGAACCGCGC GCCGCGCCAG AAGACGGACG GCAAGCCGGG CGAGCGGCGC ATGCTGAAGC TCGAGCTGAA GGTGCTCGCC GACGTCGGCC TGCTCGGCAT GCCGAACGCG GGCAAGTCGA CCTTCATCTC GTCGGTGTCG AACGCGAAGC CGAAGATCGC CGACTACCCG TTCACGACGC TCGCGCCGAA TCTCGGTGTG GTGCGGGTCG GTCCGAGCAA GAGCTTCGTG ATCGCCGATA TCCCGGGGCT GATCGAAGGC GCGGCCGAAG GCGCGGGCCT CGGGCATCAA TTCCTGCGCC ACCTGCAGCG TACCGGTGTG CTGCTGCATC TGGTCGATCT CGCGCCGTTC GACGAAAGCG TCGATCCGGT CGCGGAAGCG ACGGCGATCG TCGGCGAGCT GCGCAAGTAC GACGAGGCGC TCTACGAGAA GCCGCGCTGG CTCGTGCTCA ACAAGCTGGA CATGGTGCCG GAAGACGAGC GCGAGGCGCG CGTCGCGGAT TTCCTCGACC GCTTCGGCTG GGACGGCCCC GTGTTCGAGA TCTCGGCGCT GACGGGCCAG GGCTGCGAAG CGCTGTGCTA CGCGATCTAC GACTACCTTT CCGAGCATTC GGACGCGCAT CGCGCGGCGG AGGCGGAGGA TCTCGCGGCC GACGTGCGTT TCCGCGATGC GCCGCCGGCG AAGGGCGGTG CGACGCCGGG CGACGACGCC
Details for BuceA.00120.a.A1.GE32139
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGSVYAIAD RNINTLIDYR YAKKHLARNG ENGRGSDCYG KGGDDVTLRM PVGTIISDMD TGELIADLTE HDQRVMLAQG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MLKLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSKSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGV LLHLVDLAPF DESVDPVAEA TAIVGELRKY DEALYEKPRW LVLNKLDMVP EDEREARVAD FLDRFGWDGP VFEISALTGQ GCEALCYAIY DYLSEHSDAH RAAEAEDLAA DVRFRDAPPA KGGATPGD
Validated NT Sequence
cgtcgcccgg cgtcgcaccg cccttcgccg gcggcgcatc gcggaaacgc acgtcggccg cgagatcctc cgcctccgcc gcgcgatgcg cgtccgaatg ctcggaaagg tagtcgtaga tcgcgtagca cagcgcttcg cagccctggc ccgtcagcgc cgagatctcg aacacggggc cgtcccagcc gaagcggtcg aggaaatccg cgacgcgcgc ctcgcgctcg tcttccggca ccatgtccag cttgttgagc acgagccagc gcggcttctc gtagagcgcc tcgtcgtact tgcgcagctc gccgacgatc gccgtcgctt ccgcgaccgg atcgacgctt tcgtcgaacg gcgcgagatc gaccagatgc agcagcacac cggtacgctg caggtggcgc aggaattgat gcccgaggcc cgcgccttcg gccgcgcctt cgatcagccc cgggatatcg gcgatcacga agctcttgct cggaccgacc cgcaccacac cgagattcgg cgcgagcgtc gtgaacgggt agtcggcgat cttcggcttc gcgttcgaca ccgacgagat gaangtcgac ttgcccgcgt tcggcatgcc gagcaggccg acgtcggcga gcaccttcag ctcgagcttc agcatgcgcc gctcgcccgg cttgccgtcc gtcttctggc gcggcgcgcg gttcgtgctc gacttgaaat gcaggttgcc gaggccgccg gcgccgccct gcgcgagcat cacccgctgg tcgtgctcgg tcaggtcggc gatcagctcg cccgtgtcca tgtcgctgat gatggtgccg accggcatgc gcagcgtgac atcgtcgccg cccttgccgt agcaatccga gccgcgaccg ttttcgccgt tgcgcgcgag gtgcttcttc gcgtaacggt agtcgatcag cgtgttgatg ttgcggtcgg cgatcgcgta aacgctaccg ccgcggccgc cgtcgccacc gtccggcccg ccgaacggga cgaatttctc gcggcgcatc gacgcgctgc catcgcctcc gtcgccggcg atgacttcga ttcgtgcttc gtcaatgaac ttcatcgaac caggaccctg ggtctgagct tccagggtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFIDEARI EVIAGDGGDG SASMRREKFV PFGGPDGGDG GRGGSVYAIA DRNINTLIDY RYAKKHLARN GENGRGSDCY GKGGDDVTLR MPVGTIISDM DTGELIADLT EHDQRVMLAQ GGAGGLGNLH FKSSTNRAPR QKTDGKPGER RMLKLELKVL ADVGLLGMPN AGKSTFISSV SNAKPKIADY PFTTLAPNLG VVRVGPSKSF VIADIPGLIE GAAEGAGLGH QFLRHLQRTG VLLHLVDLAP FDESVDPVAE ATAIVGELRK YDEALYEKPR WLVLNKLDMV PEDEREARVA DFLDRFGWDG PVFEISALTG QGCEALCYAI YDYLSEHSDA HRAAEAEDLA ADVRFRDAPP AKGGATPGDD A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagttcatt gacgaagcac gaatcgaagt catcgccggc gacggaggcg atggcagcgc gtcgatgcgc cgcgagaaat tcgtcccgtt cggcgggccg gacggtggcg acggcggccg cggcggtagc gtttacgcga tcgccgaccg caacatcaac acgctgatcg actaccgtta cgcgaagaag cacctcgcgc gcaacggcga aaacggtcgc ggctcggatt gctacggcaa gggcggcgac gatgtcacgc tgcgcatgcc ggtcggcacc atcatcagcg acatggacac gggcgagctg atcgccgacc tgaccgagca cgaccagcgg gtgatgctcg cgcagggcgg cgccggcggc ctcggcaacc tgcatttcaa gtcgagcacg aaccgcgcgc cgcgccagaa gacggacggc aagccgggcg agcggcgcat gctgaagctc gagctgaagg tgctcgccga cgtcggcctg ctcggcatgc cgaacgcggg caagtcgacc ttcatctcgt cggtgtcgaa cgcgaagccg aagatcgccg actacccgtt cacgacgctc gcgccgaatc tcggtgtggt gcgggtcggt ccgagcaaga gcttcgtgat cgccgatatc ccggggctga tcgaaggcgc ggccgaaggc gcgggcctcg ggcatcaatt cctgcgccac ctgcagcgta ccggtgtgct gctgcatctg gtcgatctcg cgccgttcga cgaaagcgtc gatccggtcg cggaagcgac ggcgatcgtc ggcgagctgc gcaagtacga cgaggcgctc tacgagaagc cgcgctggct cgtgctcaac aagctggaca tggtgccgga agacgagcgc gaggcgcgcg tcgcggattt cctcgaccgc ttcggctggg acggccccgt gttcgagatc tcggcgctga cgggccaggg ctgcgaagcg ctgtgctacg cgatctacga ctacctttcc gagcattcgg acgcgcatcg cgcggcggag gcggaggatc tcgcggccga cgtgcgtttc cgcgatgcgc cgccggcgaa gggcggtgcg acgccgggcg acgacgccta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuceA.00120.a.A1.PS01116
PURIFICATION DATe: 6/15/2011
CONCENTRATION: 37.18mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGSVYAIAD RNINTLIDYR YAKKHLARNG ENGRGSDCYG KGGDDVTLRM PVGTIISDMD TGELIADLTE HDQRVMLAQG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MLKLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSKSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGV LLHLVDLAPF DESVDPVAEA TAIVGELRKY DEALYEKPRW LVLNKLDMVP EDEREARVAD FLDRFGWDGP VFEISALTGQ GCEALCYAIY DYLSEHSDAH RAAEAEDLAA DVRFRDAPPA KGGATPGD
Validated NT Sequence
cgtcgcccgg cgtcgcaccg cccttcgccg gcggcgcatc gcggaaacgc acgtcggccg cgagatcctc cgcctccgcc gcgcgatgcg cgtccgaatg ctcggaaagg tagtcgtaga tcgcgtagca cagcgcttcg cagccctggc ccgtcagcgc cgagatctcg aacacggggc cgtcccagcc gaagcggtcg aggaaatccg cgacgcgcgc ctcgcgctcg tcttccggca ccatgtccag cttgttgagc acgagccagc gcggcttctc gtagagcgcc tcgtcgtact tgcgcagctc gccgacgatc gccgtcgctt ccgcgaccgg atcgacgctt tcgtcgaacg gcgcgagatc gaccagatgc agcagcacac cggtacgctg caggtggcgc aggaattgat gcccgaggcc cgcgccttcg gccgcgcctt cgatcagccc cgggatatcg gcgatcacga agctcttgct cggaccgacc cgcaccacac cgagattcgg cgcgagcgtc gtgaacgggt agtcggcgat cttcggcttc gcgttcgaca ccgacgagat gaangtcgac ttgcccgcgt tcggcatgcc gagcaggccg acgtcggcga gcaccttcag ctcgagcttc agcatgcgcc gctcgcccgg cttgccgtcc gtcttctggc gcggcgcgcg gttcgtgctc gacttgaaat gcaggttgcc gaggccgccg gcgccgccct gcgcgagcat cacccgctgg tcgtgctcgg tcaggtcggc gatcagctcg cccgtgtcca tgtcgctgat gatggtgccg accggcatgc gcagcgtgac atcgtcgccg cccttgccgt agcaatccga gccgcgaccg ttttcgccgt tgcgcgcgag gtgcttcttc gcgtaacggt agtcgatcag cgtgttgatg ttgcggtcgg cgatcgcgta aacgctaccg ccgcggccgc cgtcgccacc gtccggcccg ccgaacggga cgaatttctc gcggcgcatc gacgcgctgc catcgcctcc gtcgccggcg atgacttcga ttcgtgcttc gtcaatgaac ttcatcgaac caggaccctg ggtctgagct tccagggtac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFIDEARI EVIAGDGGDG SASMRREKFV PFGGPDGGDG GRGGSVYAIA DRNINTLIDY RYAKKHLARN GENGRGSDCY GKGGDDVTLR MPVGTIISDM DTGELIADLT EHDQRVMLAQ GGAGGLGNLH FKSSTNRAPR QKTDGKPGER RMLKLELKVL ADVGLLGMPN AGKSTFISSV SNAKPKIADY PFTTLAPNLG VVRVGPSKSF VIADIPGLIE GAAEGAGLGH QFLRHLQRTG VLLHLVDLAP FDESVDPVAE ATAIVGELRK YDEALYEKPR WLVLNKLDMV PEDEREARVA DFLDRFGWDG PVFEISALTG QGCEALCYAI YDYLSEHSDA HRAAEAEDLA ADVRFRDAPP AKGGATPGDD A
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGTTCATT GACGAAGCAC GAATCGAAGT CATCGCCGGC GACGGAGGCG ATGGCAGCGC GTCGATGCGC CGCGAGAAAT TCGTCCCGTT CGGCGGGCCG GACGGTGGCG ACGGCGGCCG CGGCGGTAGC GTTTACGCGA TCGCCGACCG CAACATCAAC ACGCTGATCG ACTACCGTTA CGCGAAGAAG CACCTCGCGC GCAACGGCGA AAACGGTCGC GGCTCGGATT GCTACGGCAA GGGCGGCGAC GATGTCACGC TGCGCATGCC GGTCGGCACC ATCATCAGCG ACATGGACAC GGGCGAGCTG ATCGCCGACC TGACCGAGCA CGACCAGCGG GTGATGCTCG CGCAGGGCGG CGCCGGCGGC CTCGGCAACC TGCATTTCAA GTCGAGCACG AACCGCGCGC CGCGCCAGAA GACGGACGGC AAGCCGGGCG AGCGGCGCAT GCTGAAGCTC GAGCTGAAGG TGCTCGCCGA CGTCGGCCTG CTCGGCATGC CGAACGCGGG CAAGTCGACC TTCATCTCGT CGGTGTCGAA CGCGAAGCCG AAGATCGCCG ACTACCCGTT CACGACGCTC GCGCCGAATC TCGGTGTGGT GCGGGTCGGT CCGAGCAAGA GCTTCGTGAT CGCCGATATC CCGGGGCTGA TCGAAGGCGC GGCCGAAGGC GCGGGCCTCG GGCATCAATT CCTGCGCCAC CTGCAGCGTA CCGGTGTGCT GCTGCATCTG GTCGATCTCG CGCCGTTCGA CGAAAGCGTC GATCCGGTCG CGGAAGCGAC GGCGATCGTC GGCGAGCTGC GCAAGTACGA CGAGGCGCTC TACGAGAAGC CGCGCTGGCT CGTGCTCAAC AAGCTGGACA TGGTGCCGGA AGACGAGCGC GAGGCGCGCG TCGCGGATTT CCTCGACCGC TTCGGCTGGG ACGGCCCCGT GTTCGAGATC TCGGCGCTGA CGGGCCAGGG CTGCGAAGCG CTGTGCTACG CGATCTACGA CTACCTTTCC GAGCATTCGG ACGCGCATCG CGCGGCGGAG GCGGAGGATC TCGCGGCCGA CGTGCGTTTC CGCGATGCGC CGCCGGCGAA GGGCGGTGCG ACGCCGGGCG ACGACGCCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A