BuceA.00118.b

Putative UTP-glucose-1-phosphate uridylyltransferase

CENTER ID: BuceA.00118.b
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00118.b.B1.GE37380 Full length( BuceA.00118.b ) 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4737
RefSeq: YP_002233628.1
UniProt: B4EFL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKVTKAVFP VAGLGTRFLP ATKASPKEML PVVDKPLIQY AVEEAINAGI TEMIFVTGRS KRAIEDHFDK SYEIEAELEA RGKEKLLELV RGIKPSHVNC FYVRQPEALG LGHAVLCAEK LVHGEPFAVI LADDLLHGEQ PVLKQLVDVF DHYHSSVIGV ETIPREDSRS YGVVEGREWE EDIIKLSGII EKPAPEDAPS NLGVVGRYVF MPTIFDHLRK LKPGAGGELQ LTDAVQSLLA NEQVLAYRYY GTRFDCGSKL GYLKATVELA LQHPEVSREF EAYLRTCLPA LAAVA
NT Sequence
ATGTTGAAAG TCACCAAGGC CGTGTTCCCC GTTGCCGGTC TCGGCACGCG GTTCCTCCCG GCAACGAAGG CGAGCCCGAA GGAAATGCTG CCCGTCGTCG ACAAGCCGCT GATTCAGTAC GCGGTCGAGG AAGCGATCAA CGCCGGTATC ACCGAGATGA TCTTCGTCAC CGGGCGCAGC AAGCGCGCGA TCGAGGATCA TTTCGACAAG TCGTACGAGA TCGAGGCCGA GCTCGAGGCG CGCGGCAAGG AAAAGCTGCT CGAACTCGTG CGCGGCATCA AGCCGAGCCA CGTCAACTGC TTCTACGTGC GCCAGCCGGA AGCGCTCGGC CTCGGCCACG CGGTGCTGTG CGCGGAGAAG CTGGTGCACG GCGAGCCGTT CGCGGTGATC CTCGCCGACG ACCTGCTGCA CGGCGAACAG CCGGTGCTCA AGCAGCTCGT CGACGTGTTC GACCACTATC ACAGCTCGGT GATCGGGGTC GAGACGATCC CGCGCGAGGA CAGCCGCTCG TACGGCGTCG TCGAAGGCCG GGAGTGGGAA GAGGACATCA TCAAGCTGTC GGGCATCATC GAGAAGCCGG CGCCGGAGGA TGCGCCGTCG AACCTCGGCG TGGTCGGCCG CTACGTGTTC ATGCCGACGA TCTTCGATCA TCTGCGCAAG CTCAAGCCGG GCGCGGGCGG CGAATTGCAG CTCACCGACG CCGTCCAGTC GCTGCTCGCG AACGAGCAGG TGCTCGCGTA TCGCTACTAC GGCACGCGCT TCGACTGCGG CAGCAAGCTC GGCTATCTCA AGGCCACCGT CGAGCTCGCG CTCCAGCATC CGGAAGTGAG CCGCGAATTC GAGGCGTACC TGCGTACCTG CTTGCCCGCG CTGGCTGCCG TCGCT
Details for BuceA.00118.b.B1.GE37380
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAINAGITE MIFVTGRSKR AIEDHFDKSY EIEAELEARG KEKLLELVRG IKPSHVNCFY VRQPEALGLG HAVLCAEKLV HGEPFAVILA DDLLHGEQPV LKQLVDVFDH YHSSVIGVET IPREDSRSYG VVEGREWEED IIKLSGIIEK PAPEDAPSNL GVVGRYVFMP TIFDHLRKLK PGAGGELQLT DAVQSLLANE XVLAYRYYGT RFDCGSKLGY LKATVELALQ HPEVSREFEA YLRTCLPALA AVA
Validated NT Sequence
ttttntttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatgttg aaagtcacca aggccgtgtt ccccgttgcc ggtctcggca cgcggttcct cccggcaacg aaggcgagcc cgaaggaaat gctgcccgtc gtcgacaagc cgctgattca gtacgcggtc gaggaagcga tcaacgccgg tatcaccgag atgatcttcg tcaccgggcg cagcaagcgc gcgatcgagg atcatttcga caagtcgtac gagatcgagg ccgagctcga ggcgcgcggc aaggaaaagc tgctcgaact cgtgcgcggc atcaagccga gccacgtcaa ctgcttctac gtgcgccagc cggaagcgct cggcctcggc cacgcggtgc tgtgcgcgga gaagctggtg cacggcgagc cgttcgcggt gatcctcgcc gacgacctgc tgcacggcga acagccggtg ctcaagcagc tcgtcgacgt gttcgaccac tatcacagct cggtgatcgg ggtcgagacg atcccgcgcg aggacagccg ctcgtacggc gtcgtcgaag gccgggagtg ggaagaggac atcatcaagc tgtcgggcat catcgagaag ccggcgccgg aggatgcgcc gtcgaacctc ggcgtggtcg gccgctacgt gttcatgccg acgatcttcg atcatctgcg caagctcaag ccgggcgcgg gcggcgaatt gcagctcacc gacgccgtcc agtcgctgct cgcgaacgag cangtgctcg cgtatcgcta ctacggcacg cgcttcgact gcggcagcaa gctcggctat ctcaaggcca ccgtcgagct cgcgctccag catccggaag tgagccgcga attcgaggcg tacctgcgta cctgcttgcc cgcgctggct gccgtcgctt aagtgagtaa nanagnatcc nnnnnnnnnn nncccccnnn nnnnnnnnnn nnnnaaannn nnnnnnnnnn tnntnntnnn nnc
Expected Protein Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAINAGITE MIFVTGRSKR AIEDHFDKSY EIEAELEARG KEKLLELVRG IKPSHVNCFY VRQPEALGLG HAVLCAEKLV HGEPFAVILA DDLLHGEQPV LKQLVDVFDH YHSSVIGVET IPREDSRSYG VVEGREWEED IIKLSGIIEK PAPEDAPSNL GVVGRYVFMP TIFDHLRKLK PGAGGELQLT DAVQSLLANE QVLAYRYYGT RFDCGSKLGY LKATVELALQ HPEVSREFEA YLRTCLPALA AVA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga aagtcaccaa ggccgtgttc cccgttgccg gtctcggcac gcggttcctc ccggcaacga aggcgagccc gaaggaaatg ctgcccgtcg tcgacaagcc gctgattcag tacgcggtcg aggaagcgat caacgccggt atcaccgaga tgatcttcgt caccgggcgc agcaagcgcg cgatcgagga tcatttcgac aagtcgtacg agatcgaggc cgagctcgag gcgcgcggca aggaaaagct gctcgaactc gtgcgcggca tcaagccgag ccacgtcaac tgcttctacg tgcgccagcc ggaagcgctc ggcctcggcc acgcggtgct gtgcgcggag aagctggtgc acggcgagcc gttcgcggtg atcctcgccg acgacctgct gcacggcgaa cagccggtgc tcaagcagct cgtcgacgtg ttcgaccact atcacagctc ggtgatcggg gtcgagacga tcccgcgcga ggacagccgc tcgtacggcg tcgtcgaagg ccgggagtgg gaagaggaca tcatcaagct gtcgggcatc atcgagaagc cggcgccgga ggatgcgccg tcgaacctcg gcgtggtcgg ccgctacgtg ttcatgccga cgatcttcga tcatctgcgc aagctcaagc cgggcgcggg cggcgaattg cagctcaccg acgccgtcca gtcgctgctc gcgaacgagc aggtgctcgc gtatcgctac tacggcacgc gcttcgactg cggcagcaag ctcggctatc tcaaggccac cgtcgagctc gcgctccagc atccggaagt gagccgcgaa ttcgaggcgt acctgcgtac ctgcttgccc gcgctggctg ccgtcgcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat