SSGCID: Target Page

BuceA.00117.b

Catechol 1,2-dioxygenase 2

CENTER ID:	BuceA.00117.b
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00117.b.B1.GE37378	Full length( BuceA.00117.b )		1	313

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.7210
RefSeq:	YP_002153881.1
UniProt:	B4ENP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSVKVFDTRE VQDLLKAASN AGGSGTGGNA RTQQIVLRLL GDLFKAIDDL DITPDEVWAG VNYLNKLGQD GEAALLAAGL GLEKYLDIRM DAEDEAIGLD GGTPRTIEGP LYVAGAPVRD SVAKIDLDAD DGAGPLVIHG TVTGLDGKPI AGAVVECWHA NSKGFYSHFD PTGKQSDFNL RGAVKTGADG KYEFRTLMPV GYGCPPQGAT QQLLNRLGRH GNRPAHVHFF VTSDDHRKLT TQFNIDGDPL IWDDFAYATR EELIPPVTER TGGTALGMKA DAYQDIEFNF VLTPRVQGKD NQIVERLRAS AAA

NT Sequence

ATGAGCGTCA AAGTTTTCGA TACCCGGGAA GTGCAGGACC TGCTGAAGGC CGCGTCGAAC GCGGGCGGAA GCGGCACCGG CGGCAATGCA CGCACGCAGC AGATCGTGCT GCGCCTGCTC GGCGACCTGT TCAAGGCGAT CGACGATCTC GACATCACGC CCGACGAAGT GTGGGCGGGC GTCAACTACC TGAACAAGCT CGGTCAGGAT GGCGAAGCAG CGCTGCTCGC GGCCGGCCTC GGTCTCGAGA AGTATCTCGA CATCCGGATG GACGCCGAGG ACGAGGCGAT CGGGCTCGAC GGCGGCACGC CGCGCACGAT CGAGGGGCCG CTGTACGTGG CCGGAGCACC GGTGCGCGAC AGCGTCGCGA AGATCGACCT CGACGCGGAC GACGGCGCGG GCCCGCTCGT GATCCACGGC ACGGTCACCG GTCTCGACGG CAAGCCGATC GCCGGCGCGG TGGTCGAATG CTGGCACGCG AATTCGAAGG GCTTCTATTC GCACTTCGAC CCGACCGGCA AGCAGAGCGA TTTCAACCTG CGCGGCGCGG TGAAAACGGG CGCCGACGGC AAGTACGAAT TCCGCACACT GATGCCGGTC GGCTACGGCT GCCCGCCGCA GGGCGCGACG CAGCAATTGC TGAACCGCCT CGGCCGCCAC GGCAACCGTC CGGCGCACGT GCACTTCTTC GTGACGAGCG ACGATCACCG CAAGCTGACG ACACAGTTCA ACATCGACGG CGATCCGCTG ATCTGGGACG ACTTCGCGTA TGCGACGCGC GAGGAATTGA TCCCGCCCGT GACCGAAAGA ACCGGCGGCA CGGCGCTCGG CATGAAGGCC GATGCGTACC AGGACATCGA GTTCAACTTC GTGCTGACGC CGCGCGTGCA GGGCAAGGAC AACCAGATCG TCGAACGCCT GCGCGCGTCC GCCGCGGCA

Details for BuceA.00117.b.B1.GE37378

HARVESTED ON:	9/27/2013
SEQUENCED ON:	10/3/2013
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	93

Validated AA Sequence

MAHHHHHHMS VKVFDTREVQ DLLKAASNAG GSGTGGNART QQIVLRLLGD LFKAIDDLDI TPDEVWAGVN YLNKLGQDGE AALLAAGLGL EKYLDIRMDA EDEAIGLDGG TPRTIEGPLY VAGAPVRDSV AKIDLDADDG AGPLVIHGTV TGLDGKPIAG AVVECWHANS KGFYSHFDPT GKQSDFNLRG AVKTGADGKY EFRTLMPVGY GCPPQGATQQ LLNRLGRHGN RPAHVHFFVT SDDHRKLTTQ FNIDGDPLIW DDFAYATREE LIPPVTERTG GTALGMKADA YXXSSSTSC

Validated NT Sequence

ttttgnttna ctttaagaag gagatatacc atggctcacc accaccacca ccatatgagc gtcaaagttt tcgatacccg ggaagtgcag gacctgctga aggccgcgtc gaacgcgggc ggaagcggca ccggcggcaa tgcacgcacg cagcagatcg tgctgcgcct gctcggcgac ctgttcaagg cgatcgacga tctcgacatc acgcccgacg aagtgtgggc gggcgtcaac tacctgaaca agctcggtca ggatggcgaa gcagcgctgc tcgcggccgg cctcggtctc gagaagtatc tcgacatccg gatggacgcc gaggacgagg cgatcgggct cgacggcggc acgccgcgca cgatcgaggg gccgctgtac gtggccggag caccggtgcg cgacagcgtc gcgaagatcg acctcgacgc ggacgacggc gcgggcccgc tcgtgatcca cggcacggtc accggtctcg acggcaagcc gatcgccggc gcggtggtcg aatgctggca cgcgaattcg aagggcttct attcgcactt cgacccgacc ggcaagcaga gcgatttcaa cctgcgcggc gcggtgaaaa cgggcgccga cggcaagtac gaattccgca cactgatgcc ggtcggctac ggctgcccgc cgcagggcgc gacgcagcaa ttgctgaacc gcctcggccg ccacggcaac cgtccggcgc acgtgcactt cttcgtgacg agcgacgatc accgcaagct gacgacacag ttcaacatcg acggcgatcc gctgatctgg gacgacttcg cgtatgcgac gcgcgaggaa ttgatcccgc ccgtgaccga aagaaccggc ggcacggcgc tcggcatgaa ggccgatgcg taccnnncat cgagttcaac ttcntgctga cgccgcgcgt gcagggcaag gacaaccaga tcgtcnaacg cctgcgcgcg tccncncggc atnantgagt annnnnnnnn nnnntnnnnn nnnnnnnnnn nnnnncnnnn nnnnnnnnnn nnnnnnnnnn nnntnnnnnn nnnnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg tcaaagtttt cgatacccgg gaagtgcagg acctgctgaa ggccgcgtcg aacgcgggcg gaagcggcac cggcggcaat gcacgcacgc agcagatcgt gctgcgcctg ctcggcgacc tgttcaaggc gatcgacgat ctcgacatca cgcccgacga agtgtgggcg ggcgtcaact acctgaacaa gctcggtcag gatggcgaag cagcgctgct cgcggccggc ctcggtctcg agaagtatct cgacatccgg atggacgccg aggacgaggc gatcgggctc gacggcggca cgccgcgcac gatcgagggg ccgctgtacg tggccggagc accggtgcgc gacagcgtcg cgaagatcga cctcgacgcg gacgacggcg cgggcccgct cgtgatccac ggcacggtca ccggtctcga cggcaagccg atcgccggcg cggtggtcga atgctggcac gcgaattcga agggcttcta ttcgcacttc gacccgaccg gcaagcagag cgatttcaac ctgcgcggcg cggtgaaaac gggcgccgac ggcaagtacg aattccgcac actgatgccg gtcggctacg gctgcccgcc gcagggcgcg acgcagcaat tgctgaaccg cctcggccgc cacggcaacc gtccggcgca cgtgcacttc ttcgtgacga gcgacgatca ccgcaagctg acgacacagt tcaacatcga cggcgatccg ctgatctggg acgacttcgc gtatgcgacg cgcgaggaat tgatcccgcc cgtgaccgaa agaaccggcg gcacggcgct cggcatgaag gccgatgcgt accaggacat cgagttcaac ttcgtgctga cgccgcgcgt gcagggcaag gacaaccaga tcgtcgaacg cctgcgcgcg tccgccgcgg catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat