SSGCID: Target Page

BuceA.00117.a

Putative hydroxyquinol 1,2-dioxygenase

CENTER ID:	BuceA.00117.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00117.a.B1.GE37377	Full length( BuceA.00117.a )		1	293

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.1388
RefSeq:	YP_002230472.1
UniProt:	B4E663

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRNLNEDTIT QAVIASLSGC RDERLRTVMT SLVQHLHSFA RETKLTEAEW QAAIGFLTAV GRITDDKRQE FILLSDVLGL STLVTAQNHA KPAGCTEATV FGPFYVDGSP EFGMFDDIAN GACGEPCFVS GQVRGIDGTP VAHASLEVWQ ADEDGHYDVQ LPGDDGSVTH RARGRLRSGA DGRYAFRSIL AEPYPIPHDG PVGAMLDALG RHPWRPAHLH FMIEARGYET LITHVFRDGD RYLDSDAVFG VRSTLVADWV RHAPGIAPDG SRMDTPFYTL DYDFVLNRAE RAA

NT Sequence

ATGCGCAATC TCAACGAAGA CACCATCACG CAGGCCGTGA TCGCGTCGCT CTCCGGCTGT CGCGACGAGC GGCTGCGCAC GGTGATGACG AGCCTCGTCC AGCACCTGCA TTCGTTCGCC CGCGAAACGA AGCTCACCGA GGCCGAGTGG CAGGCCGCGA TCGGCTTCCT GACGGCCGTC GGCCGCATCA CCGACGACAA GCGCCAGGAG TTCATCCTGT TGTCCGACGT GCTCGGGCTG TCGACGCTCG TGACCGCGCA GAACCATGCA AAGCCGGCCG GCTGCACGGA GGCGACCGTG TTCGGGCCGT TCTACGTCGA CGGCAGCCCC GAATTCGGGA TGTTCGACGA CATCGCGAAC GGCGCATGCG GCGAGCCGTG CTTCGTGTCG GGCCAGGTAC GCGGCATCGA CGGCACGCCG GTCGCGCATG CGTCGCTGGA AGTGTGGCAA GCCGACGAGG ACGGCCACTA CGACGTGCAG TTGCCTGGCG ACGACGGGTC GGTCACGCAT CGCGCGCGCG GCCGGCTGCG CAGCGGCGCC GACGGCCGCT ATGCGTTCCG CTCGATCCTC GCCGAGCCGT ATCCGATTCC GCACGACGGC CCCGTCGGCG CGATGCTCGA TGCGCTCGGC CGCCATCCGT GGCGCCCCGC CCACCTGCAT TTCATGATCG AAGCGCGCGG CTACGAAACG CTGATCACGC ACGTGTTCCG CGACGGCGAC CGCTATCTCG ACTCCGATGC GGTGTTCGGC GTGCGTTCGA CGCTGGTCGC CGACTGGGTC CGGCATGCGC CCGGCATCGC GCCCGACGGC TCGCGCATGG ACACGCCGTT CTACACGCTC GACTACGACT TCGTGCTCAA TCGCGCGGAG CGTGCCGCA

Details for BuceA.00117.a.B1.GE37377

HARVESTED ON:	9/27/2013
SEQUENCED ON:	10/3/2013
EXPECTED MW:	33kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR NLNEDTITQA VIASLSGCRD ERLRTVMTSL VQHLHSFARE TKLTEAEWQA AIGFLTAVGR ITDDKRQEFI LLSDVLGLST LVTAQNHAKP AGCTEATVFG PFYVDGSPEF GMFDDIANGA CGEPCFVSGQ VRGIDGTPVA HASLEVWQAD EDGHYDVQLP GDDGSVTHRA RGRLRSGADG RYAFRSILAE PYPIPHDGPV GAMLDALGRH PWRPAHLHFM IEARGYETLI THVFRDGDRY LDSDAVFGVR STLVADWVRH APGIAPDGSR MDTPFYTLDY DFXLNRAXRA A

Validated NT Sequence

antttgtttn actttaagaa ggaganatac catggctcac caccaccacc accatatgcg caatctcaac gaagacacca tcacgcaggc cgtgatcgcg tcgctctccg gctgtcgcga cgagcggctg cgcacggtga tgacgagcct cgtccagcac ctgcattcgt tcgcccgcga aacgaagctc accgaggccg agtggcaggc cgcgatcggc ttcctgacgg ccgtcggccg catcaccgac gacaagcgcc aggagttcat cctgttgtcc gacgtgctcg ggctgtcgac gctcgtgacc gcgcagaacc atgcaaagcc ggccggctgc acggaggcga ccgtgttcgg gccgttctac gtcgacggca gccccgaatt cgggatgttc gacgacatcg cgaacggcgc atgcggcgag ccgtgcttcg tgtcgggcca ggtacgcggc atcgacggca cgccggtcgc gcatgcgtcg ctggaagtgt ggcaagccga cgaggacggc cactacgacg tgcagttgcc tggcgacgac gggtcggtca cgcatcgcgc gcgcggccgg ctgcgcagcg gcgccgacgg ccgctatgcg ttccgctcga tcctcgccga gccgtatccg attccgcacg acggccccgt cggcgcgatg ctcgatgcgc tcggccgcca tccgtggcgc cccgcccacc tgcatttcat gatcgaagcg cgcggctacg aaacgctgat cacgcacgtg ttccgcgacg gcgaccgcta tctcgactcc gatgcggtgt tcggcgtgcg ttcgacgctg gtcgccgact gggtccggca tgcgcccggc atcgcgcccg acggctcgcg catggacacg ccgttctaca cgctcgacta cgacttcntg ctcaatcgcg cggancgtgc cgcataagtg agtananagg anccnnnnnn aaannnnnnn nnnnnnnnnn gnnnnnnnnn nnnnngnnnn ntntnnttnn t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgca atctcaacga agacaccatc acgcaggccg tgatcgcgtc gctctccggc tgtcgcgacg agcggctgcg cacggtgatg acgagcctcg tccagcacct gcattcgttc gcccgcgaaa cgaagctcac cgaggccgag tggcaggccg cgatcggctt cctgacggcc gtcggccgca tcaccgacga caagcgccag gagttcatcc tgttgtccga cgtgctcggg ctgtcgacgc tcgtgaccgc gcagaaccat gcaaagccgg ccggctgcac ggaggcgacc gtgttcgggc cgttctacgt cgacggcagc cccgaattcg ggatgttcga cgacatcgcg aacggcgcat gcggcgagcc gtgcttcgtg tcgggccagg tacgcggcat cgacggcacg ccggtcgcgc atgcgtcgct ggaagtgtgg caagccgacg aggacggcca ctacgacgtg cagttgcctg gcgacgacgg gtcggtcacg catcgcgcgc gcggccggct gcgcagcggc gccgacggcc gctatgcgtt ccgctcgatc ctcgccgagc cgtatccgat tccgcacgac ggccccgtcg gcgcgatgct cgatgcgctc ggccgccatc cgtggcgccc cgcccacctg catttcatga tcgaagcgcg cggctacgaa acgctgatca cgcacgtgtt ccgcgacggc gaccgctatc tcgactccga tgcggtgttc ggcgtgcgtt cgacgctggt cgccgactgg gtccggcatg cgcccggcat cgcgcccgac ggctcgcgca tggacacgcc gttctacacg ctcgactacg acttcgtgct caatcgcgcg gagcgtgccg catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat