SSGCID: Target Page

BuceA.00116.a

4-hydroxythreonine-4-phosphate dehydrogenase

CENTER ID:	BuceA.00116.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00116.a.B1.GE43977	Full length( BuceA.00116.a )		1	329

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.940
RefSeq:	YP_002230050.1
UniProt:	B4EBE7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTPALQIAI TTGEPAGVGP ELTVQALRDA AQRWPDAHFT VLGDAALLDA RAAAVGIDPA TLAGGAQVSV AHRALAVPAQ AGKLDAANGR YVLGLLDAAI DGALAGRYDA IVTAPLQKST INDAGVPFTG HTEYLAERTH TPRVVMMLAG TGQRPLRVAL ATTHLPLKDV SAALTIDGLV ETLAIIDRDL RRDFGLATPR ILVTGLNPHA GENGYLGREE IDVISPALAR AHAQHIDARG PYPADTLFQP RHLADADCVL AMFHDQGLPV LKYATFGEGI NVTLGLPIIR TSVDHGTALD LAGTGRADPG SMIAALDTAV TMARHRRAA

NT Sequence

ATGACCACGC CCGCGCTGCA GATCGCGATC ACGACCGGCG AACCCGCGGG AGTCGGCCCG GAGCTGACCG TGCAGGCGCT GCGTGACGCC GCGCAGCGTT GGCCCGACGC GCATTTCACC GTGCTCGGCG ATGCGGCGTT GCTCGACGCG CGGGCCGCGG CGGTCGGCAT CGACCCGGCC ACGCTGGCCG GCGGCGCGCA GGTGTCGGTC GCGCATCGCG CGCTGGCCGT GCCCGCGCAG GCGGGCAAGC TCGACGCGGC GAACGGCCGC TACGTGCTCG GGCTGCTCGA CGCGGCGATC GACGGCGCGC TGGCGGGCCG GTACGACGCG ATCGTCACCG CGCCGCTGCA GAAAAGCACG ATCAACGATG CGGGCGTGCC GTTCACCGGC CATACCGAAT ACCTGGCCGA GCGCACGCAT ACGCCGCGCG TCGTGATGAT GCTGGCCGGC ACCGGCCAGC GGCCGCTACG TGTCGCGCTC GCGACCACCC ACTTGCCGCT GAAGGACGTA TCGGCCGCGC TGACGATCGA CGGGCTCGTC GAGACGCTCG CGATCATCGA TCGTGACCTG CGGCGCGACT TCGGTCTCGC CACGCCGCGC ATCCTCGTGA CGGGCCTGAA CCCGCACGCG GGCGAGAACG GTTATCTCGG CCGCGAGGAG ATCGACGTGA TCTCGCCGGC GCTGGCCCGT GCGCATGCGC AACACATCGA TGCGCGCGGC CCGTATCCGG CCGACACGCT GTTCCAGCCG CGCCATCTGG CCGATGCCGA TTGCGTGCTG GCGATGTTCC ATGACCAGGG CCTGCCCGTG CTGAAGTACG CGACGTTCGG CGAGGGCATC AACGTGACGC TCGGGCTGCC GATCATCCGC ACGTCGGTCG ATCACGGCAC CGCGCTCGAT CTGGCCGGCA CGGGCCGCGC CGATCCGGGC AGCATGATCG CCGCGCTCGA TACGGCCGTC ACGATGGCGC GCCATCGCCG CGCAGCC

Details for BuceA.00116.a.B1.GE43977

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/16/2020
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT TPALQIAITT GEPAGVGPEL TVQALRDAAQ RWPDAHFTVL GDAALLDARA AAVGIDPATL AGGAQVSVAH RALAVPAQAG KLDAANGRYV LGLLDAAIDG ALAGRYDAIV TAPLQKSTIN DAGVPFTGHT EYLAERTHTP RVVMMLAGTG QRPLRVALAT THLPLKDVSA ALTIDGLVET LAIIDRDLRR DFGLATPRIL VTGLNPHAGE NGYLGREEID VISPALARAH AQHIDARGPY PADTLFQPRH LADADCVLAM FHDQGLPVLK YATFGEGINV TLGLPIIRTS VDHGTALDLA GTGRADPGSM IAALDTAVTM ARHRRAA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca cgcccgcgct gcagatcgcg atcacgaccg gcgaacccgc gggagtcggc ccggagctga ccgtgcaggc gctgcgtgac gccgcgcagc gttggcccga cgcgcatttc accgtgctcg gcgatgcggc gttgctcgac gcgcgggccg cggcggtcgg catcgacccg gccacgctgg ccggcggcgc gcaggtgtcg gtcgcgcatc gcgcgctggc cgtgcccgcg caggcgggca agctcgacgc ggcgaacggc cgctacgtgc tcgggctgct cgacgcggcg atcgacggcg cgctggcggg ccggtacgac gcgatcgtca ccgcgccgct gcagaaaagc acgatcaacg atgcgggcgt gccgttcacc ggccataccg aatacctggc cgagcgcacg catacgccgc gcgtcgtgat gatgctggcc ggcaccggcc agcggccgct acgtgtcgcg ctcgcgacca cccacttgcc gctgaaggac gtatcggccg cgctgacgat cgacgggctc gtcgagacgc tcgcgatcat cgatcgtgac ctgcggcgcg acttcggtct cgccacgccg cgcatcctcg tgacgggcct gaacccgcac gcgggcgaga acggttatct cggccgcgag gagatcgacg tgatctcgcc ggcgctggcc cgtgcgcatg cgcaacacat cgatgcgcgc ggcccgtatc cggccgacac gctgttccag ccgcgccatc tggccgatgc cgattgcgtg ctggcgatgt tccatgacca gggcctgccc gtgctgaagt acgcgacgtt cggcgagggc atcaacgtga cgctcgggct gccgatcatc cgcacgtcgg tcgatcacgg caccgcgctc gatctggccg gcacgggccg cgccgatccg ggcagcatga tcgccgcgct cgatacggcc gtcacgatgg cgcgccatcg ccgcgcagcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat