SSGCID: Target Page

BuceA.00112.b

3-methyl-2-oxobutanoate hydroxymethyltransferase

CENTER ID:	BuceA.00112.b
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00112.b.A1.GE33195	Full length( BuceA.00112.b )		1	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.4161
RefSeq:	YP_002233103.1
UniProt:	B4EJY2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAHTRITRK TVTAIRSTKG IGSLVSLTAY SAPMAKLVDD VADVIIVGDS VGMVLYGMPD TLRVTLDMMI AHGAAVVRGA AQACVVVDLP FSTFQESPAQ AYRSAARLLA ETGAQAVKLE GGCEMTDTIR FLTDRGIPVM AHVGLMPQQA NATGGFRAQG MDPRSAAQVF DAACAAERAG AFGVVIEGTA EALARHLTET LTIPTIGIGA SPACDGQVLV TEDMIGAFDA YTPRFVKRYA DANAVMRDAI RQYAHDVRQR VFPEPAHCFG YGKPLQLADA APAA

NT Sequence

ATGAGCGCTC ATACCCGCAT CACCCGCAAG ACCGTCACCG CGATCCGCTC GACCAAAGGC ATCGGCAGCC TCGTGTCGCT GACCGCGTAT TCCGCGCCGA TGGCGAAACT CGTCGACGAC GTGGCCGACG TGATCATCGT CGGCGACTCC GTCGGCATGG TGCTGTACGG GATGCCCGAT ACGCTGCGCG TCACGCTCGA CATGATGATT GCACACGGGG CCGCCGTCGT GCGCGGCGCC GCGCAGGCGT GCGTCGTCGT CGACCTGCCG TTCTCCACGT TCCAGGAATC ACCCGCGCAG GCGTATCGCT CCGCCGCGCG GCTGCTCGCC GAAACCGGCG CGCAGGCCGT GAAGCTCGAA GGCGGCTGCG AGATGACCGA CACGATCCGC TTCCTGACCG ATCGCGGCAT TCCGGTGATG GCACATGTCG GCCTCATGCC GCAGCAGGCC AACGCGACGG GCGGCTTCCG TGCGCAGGGC ATGGACCCGC GTTCGGCCGC GCAGGTGTTC GACGCCGCGT GCGCGGCCGA GCGGGCCGGC GCGTTCGGCG TCGTCATCGA AGGCACCGCC GAAGCGCTCG CGCGCCACCT GACCGAAACG CTGACGATCC CGACGATCGG CATCGGCGCA TCGCCCGCGT GCGACGGGCA GGTGCTCGTG ACCGAGGACA TGATCGGCGC GTTCGATGCG TACACGCCGC GCTTCGTGAA GCGTTACGCG GATGCGAACG CGGTGATGCG CGACGCGATC CGCCAGTACG CGCACGACGT GCGGCAGCGC GTGTTTCCCG AGCCCGCGCA TTGCTTCGGA TACGGCAAGC CGCTGCAACT GGCGGACGCG GCCCCGGCTG CG

Details for BuceA.00112.b.A1.GE33195

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	109

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSAHTRITR KTVTAIRSTK GIGSLVSLTA YSAPMAKLVD DVADVIIVGD SVGMVLYGMP DTLRVTLDMM IAHGAAVVRG AAQACVVVDL PFSTFQESPA QAYRSAARLL AETGAQAVKL EGGCEMTDTI RFLTDRGIPV MAHVGLMPQQ ANATGGFRAQ GMDPRSAAQV FDAACAAERA GAFGVVIEGT AEALARHLTE TLTIPTIGIG ASPACDGQVL VTEDMIGAFD AYTPRFVKRY ADANAVMRDA IRQYAHDVRQ RVFPEPAHCF XXGKPLQXGG XGPGCVINST NKFCSXLLEX XXATKPERXL XLAX

Validated NT Sequence

aaggagnnat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga gcgctcatac ccgcatcacc cgcaagaccg tcaccgcgat ccgctcgacc aaaggcatcg gcagcctcgt gtcgctgacc gcgtattccg cgccgatggc gaaactcgtc gacgacgtgg ccgacgtgat catcgtcggc gactccgtcg gcatggtgct gtacgggatg cccgatacgc tgcgcgtcac gctcgacatg atgattgcac acggggccgc cgtcgtgcgc ggcgccgcgc aggcgtgcgt cgtcgtcgac ctgccgttct ccacgttcca ggaatcaccc gcgcaggcgt atcgctccgc cgcgcggctg ctcgccgaaa ccggcgcgca ggccgtgaag ctcgaaggcg gctgcgagat gaccgacacg atccgcttcc tgaccgatcg cggcattccg gtgatggcac atgtcggcct catgccgcag caggccaacg cgacgggcgg cttccgtgcg cagggcatgg acccgcgttc ggccgcgcag gtgttcgacg ccgcgtgcgc ggccgagcgg gccggcgcgt tcggcgtcgt catcgaaggc accgccgaag cgctcgcgcg ccacctgacc gaaacgctga cgatcccgac gatcggcatc ggcgcatcgc ccgcgtgcga cgggcaggtg ctcgtgaccg aggacatgat cggcgcgttc gatgcgtaca cgccgcgctt cgtgaagcgt tacgcggatg cgaacgcggt gatgcgcgac gcgatccgcc agtacgcgca cgacgtgcgg cagcgcgtgt ttcccgagcc cgcgcattgc ttcgnatang gcaagccgct gcaacnnggc ggangcggcc ccggctgcgt aataaacagc acgaacaagt tctgcagccn ncttctcgag ganncnnctg ctacaaagcc cgaaaggnan ctgnnnttgg ctnnng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgctcat acccgcatca cccgcaagac cgtcaccgcg atccgctcga ccaaaggcat cggcagcctc gtgtcgctga ccgcgtattc cgcgccgatg gcgaaactcg tcgacgacgt ggccgacgtg atcatcgtcg gcgactccgt cggcatggtg ctgtacggga tgcccgatac gctgcgcgtc acgctcgaca tgatgattgc acacggggcc gccgtcgtgc gcggcgccgc gcaggcgtgc gtcgtcgtcg acctgccgtt ctccacgttc caggaatcac ccgcgcaggc gtatcgctcc gccgcgcggc tgctcgccga aaccggcgcg caggccgtga agctcgaagg cggctgcgag atgaccgaca cgatccgctt cctgaccgat cgcggcattc cggtgatggc acatgtcggc ctcatgccgc agcaggccaa cgcgacgggc ggcttccgtg cgcagggcat ggacccgcgt tcggccgcgc aggtgttcga cgccgcgtgc gcggccgagc gggccggcgc gttcggcgtc gtcatcgaag gcaccgccga agcgctcgcg cgccacctga ccgaaacgct gacgatcccg acgatcggca tcggcgcatc gcccgcgtgc gacgggcagg tgctcgtgac cgaggacatg atcggcgcgt tcgatgcgta cacgccgcgc ttcgtgaagc gttacgcgga tgcgaacgcg gtgatgcgcg acgcgatccg ccagtacgcg cacgacgtgc ggcagcgcgt gtttcccgag cccgcgcatt gcttcggata cggcaagccg ctgcaactgg cggacgcggc cccggctgcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa