BuceA.00109.c

Putative N-isopropylammelide isopropyl amidohydrolase

CENTER ID: BuceA.00109.c
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00109.c.B1.GE37374 Full length( BuceA.00109.c ) 1 399
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6611
RefSeq: YP_002235380.1
UniProt: B4ELH8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTERFFINAV DAGGQPVNLV VRDGRFAAIG ADCAAAPGAQ TIDLDGRVVL PGFVDGHIHL DKSFVGDRWV PHEPAGSLRE RLAVEKRQLA AAPPIVERAN ALIAQAAAFG TIAMRSHVDV DATTGLSNLQ AVMAAREQWR GIVDIELVAF PQAGVITCPG TAEILDAAVR EGADVVGGID PTTLDGDADG QLDIVFGIAE KRGAKIDIHL HEPGETGIAQ LLRIAARTRA AGLGGRVNVS HAYALGQVSD DDVQRTAAAL ADAGVSILTN APGDCAFPPV QRLRDAGVHV FAGNDNIRDA WWPYGNGDML QRAMMVGYRS GFYTDEALGI ALDMATHAGA RAIGKENYGI AVGCDASFVA VRAPNAAAAV AGVPAERWVF RRGESDTGAP FAPSLRFTR
NT Sequence
ATGACCGAAC GATTCTTCAT CAACGCCGTC GACGCCGGCG GCCAGCCCGT CAATCTCGTC GTCCGTGACG GCCGCTTCGC CGCGATCGGC GCCGATTGCG CGGCCGCGCC GGGCGCGCAG ACGATCGACC TCGACGGTCG CGTCGTGCTG CCCGGTTTCG TCGACGGCCA TATTCATCTC GACAAGAGCT TCGTCGGCGA TCGCTGGGTG CCGCATGAAC CGGCCGGCTC GCTGCGCGAG CGGCTCGCGG TCGAGAAGCG CCAGCTCGCG GCGGCGCCGC CGATCGTCGA GCGTGCGAAC GCGCTGATCG CGCAGGCCGC CGCGTTCGGC ACGATCGCGA TGCGCAGCCA CGTCGACGTC GATGCGACGA CGGGCCTGTC GAACCTGCAG GCCGTGATGG CCGCGCGCGA ACAATGGCGT GGCATCGTCG ACATCGAACT GGTCGCGTTT CCGCAGGCCG GCGTGATCAC GTGCCCGGGC ACGGCCGAGA TCCTCGATGC AGCCGTGCGC GAAGGCGCGG ACGTGGTCGG CGGGATCGAC CCGACGACGC TCGACGGCGA CGCGGACGGC CAGCTCGACA TCGTGTTCGG CATCGCCGAG AAGCGCGGCG CGAAAATCGA CATCCACCTG CACGAGCCGG GCGAGACAGG CATCGCGCAA CTGCTGCGGA TCGCGGCGCG CACGCGCGCG GCCGGGCTCG GCGGGCGCGT GAACGTGAGC CACGCGTACG CGCTGGGCCA GGTGAGCGAC GACGACGTGC AGCGCACCGC GGCGGCCTTG GCCGACGCCG GGGTATCGAT CCTGACGAAT GCGCCGGGCG ACTGCGCGTT TCCGCCGGTG CAACGGCTGC GCGACGCGGG CGTGCACGTG TTCGCGGGCA ACGACAACAT TCGCGACGCA TGGTGGCCGT ACGGCAACGG CGACATGCTG CAGCGCGCGA TGATGGTCGG CTACCGGTCG GGCTTCTACA CCGACGAAGC GCTCGGCATC GCACTCGACA TGGCGACGCA CGCCGGCGCG CGCGCGATCG GTAAGGAAAA CTATGGAATC GCGGTCGGCT GCGACGCGAG CTTCGTCGCG GTGCGCGCGC CGAATGCGGC GGCGGCCGTC GCGGGCGTGC CGGCCGAACG CTGGGTATTC CGCCGCGGCG AAAGCGATAC GGGCGCGCCG TTCGCCCCGT CGCTGCGCTT CACGCGC
Details for BuceA.00109.c.B1.GE37374
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 94
Validated AA Sequence
MAHHHHHHMT ERFFINAVDA GGQPVNLVVR DGRFAAIGAD CAAAPGAQTI DLDGRVVLPG FVDGHIYLDK SFVGDRWVPH EPAGSLRERL AVEKRQLAAA PPIVERANAL IAQAAAFGTI AMRSHVDVDA TTGLSNLQAV MAAREQWRGI VDIELVAFPQ AGVITCPGTA EILDAAVREG ADVVGGIDPT TLDGDADGQL DIVFGIAEKR GAKIDIHLHE PGETGIAQLL RIAARTRAAG LGGRVNVSHA YALGQVSDDD VQRTAAALAD AGVSILTNAP GDCAFPPVQR LRDAGVHVFA GNXXFATHGX RTATATAAAX DXGRLXXRAX TXXXSXSXSX XXXXXXXXXX XXXXXXXXXX XXXXXXXXGX XXXXXXXXXX XXX
Validated NT Sequence
attttnttta nctttaagaa ggaganatac catggctcac caccaccacc accatatgac cgaacgattc ttcatcaacg ccgtcgacgc cggcggccag cccgtcaatc tcgtcgtccg tgacggccgc ttcgccgcga tcggcgccga ttgcgcggcc gcgccgggcg cgcagacgat cgacctcgac ggtcgcgtcg tgctgcccgg tttcgtcgac ggccatattt atctcgacaa gagcttcgtc ggcgatcgct gggtgccgca tgaaccggcc ggctcgctgc gcgagcggct cgcggtcgag aagcgccagc tcgcggcggc gccgccgatc gtcgagcgtg cgaacgcgct gatcgcgcag gccgccgcgt tcggcacgat cgcgatgcgc agccacgtcg acgtcgatgc gacgacgggc ctgtcgaacc tgcaggccgt gatggccgcg cgcgaacaat ggcgtggcat cgtcgacatc gaactggtcg cgtttccgca ggccggcgtg atcacgtgcc cgggcacggc cgagatcctc gatgcagccg tgcgcgaagg cgcggacgtg gtcggcggga tcgacccgac gacgctcgac ggcgacgcgg acggccagct cgacatcgtg ttcggcatcg ccgagaagcg cggcgcgaaa atcgacatcc acctgcacga gccgggcgag acaggcatcg cgcaactgct gcggatcgcg gcgcgcacgc gcgcggccgg gctcggcggg cgcgtgaacg tgagccacgc gtacgcgctg ggccaggtga gcgacgacga cgtgcagcgc accgcggcgg ccttggccga cgccggggta tcgatcctga cgaatgcgcc gggcgactgc gcgtttccgc cggtgcaacg gctgcgcgac gcgggcgtgc acgtgttcgc gggcaacgan nnattcgcga cgcatggnng ccgtacggca acggcgactg ctgcagcgng cgatganggn cggctancng ntcgggctnc tacncnanan cnntcggnnt cnnantcnnc atnnnncnna nnnnncnnnn nnnnnnatnn nnnnaannnn nnnncnnnnn ngnnnnnnnn nnnnnntnnn nnnnnncnaa nnnnnggnnn nnnnnnngnn nnncncnnnn nnnnnnnggn nnnnnnnnnc c
Expected Protein Sequence
MAHHHHHHMT ERFFINAVDA GGQPVNLVVR DGRFAAIGAD CAAAPGAQTI DLDGRVVLPG FVDGHIHLDK SFVGDRWVPH EPAGSLRERL AVEKRQLAAA PPIVERANAL IAQAAAFGTI AMRSHVDVDA TTGLSNLQAV MAAREQWRGI VDIELVAFPQ AGVITCPGTA EILDAAVREG ADVVGGIDPT TLDGDADGQL DIVFGIAEKR GAKIDIHLHE PGETGIAQLL RIAARTRAAG LGGRVNVSHA YALGQVSDDD VQRTAAALAD AGVSILTNAP GDCAFPPVQR LRDAGVHVFA GNDNIRDAWW PYGNGDMLQR AMMVGYRSGF YTDEALGIAL DMATHAGARA IGKENYGIAV GCDASFVAVR APNAAAAVAG VPAERWVFRR GESDTGAPFA PSLRFTR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg aacgattctt catcaacgcc gtcgacgccg gcggccagcc cgtcaatctc gtcgtccgtg acggccgctt cgccgcgatc ggcgccgatt gcgcggccgc gccgggcgcg cagacgatcg acctcgacgg tcgcgtcgtg ctgcccggtt tcgtcgacgg ccatattcat ctcgacaaga gcttcgtcgg cgatcgctgg gtgccgcatg aaccggccgg ctcgctgcgc gagcggctcg cggtcgagaa gcgccagctc gcggcggcgc cgccgatcgt cgagcgtgcg aacgcgctga tcgcgcaggc cgccgcgttc ggcacgatcg cgatgcgcag ccacgtcgac gtcgatgcga cgacgggcct gtcgaacctg caggccgtga tggccgcgcg cgaacaatgg cgtggcatcg tcgacatcga actggtcgcg tttccgcagg ccggcgtgat cacgtgcccg ggcacggccg agatcctcga tgcagccgtg cgcgaaggcg cggacgtggt cggcgggatc gacccgacga cgctcgacgg cgacgcggac ggccagctcg acatcgtgtt cggcatcgcc gagaagcgcg gcgcgaaaat cgacatccac ctgcacgagc cgggcgagac aggcatcgcg caactgctgc ggatcgcggc gcgcacgcgc gcggccgggc tcggcgggcg cgtgaacgtg agccacgcgt acgcgctggg ccaggtgagc gacgacgacg tgcagcgcac cgcggcggcc ttggccgacg ccggggtatc gatcctgacg aatgcgccgg gcgactgcgc gtttccgccg gtgcaacggc tgcgcgacgc gggcgtgcac gtgttcgcgg gcaacgacaa cattcgcgac gcatggtggc cgtacggcaa cggcgacatg ctgcagcgcg cgatgatggt cggctaccgg tcgggcttct acaccgacga agcgctcggc atcgcactcg acatggcgac gcacgccggc gcgcgcgcga tcggtaagga aaactatgga atcgcggtcg gctgcgacgc gagcttcgtc gcggtgcgcg cgccgaatgc ggcggcggcc gtcgcgggcg tgccggccga acgctgggta ttccgccgcg gcgaaagcga tacgggcgcg ccgttcgccc cgtcgctgcg cttcacgcgc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat