SSGCID: Target Page

BuceA.00107.g

Multidrug efflux system transport protein

CENTER ID:	BuceA.00107.g
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00107.g.A2.GE32143	Mature protein( BuceA.00107.g )		24	412

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00107.g.A2.PS01155	Mature protein( BuceA.00107.g )		24	412

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.7472
RefSeq:	YP_002154137.1
UniProt:	B4EPF4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRVERVPYRL ITAATAAVFL AACGKKESAP PPQTPEVGVV TVQPQAVPAV TELPGRTSAF LVAQVRARVD GIVLRREFTE GTDVKAGQRL YKIDPAPYVA ALNSAKATLA KAQANLVTQN ALVARYKVLV AANAVSKQDY DNAVATQGQA AADVAAGKAA VDTAQINLGY TDVVSPITGR VGISQVTPGA YVQASQATLM STVQQLDPVY VDLTQSSLEG LKLRQDVQSG RLKTTGPGAA KVSLILEDGK TYSEPGKLQF SDVTVDQTTG SVTIRAIFPN PGRVLLPGMF VRARIEEGVN ENAFLVPQIG VTHDQKGQAI ALVVTAANKV EMRPLTTTGT YGQNWIVEGG LQAGDHVIVQ GVDKVRPGAT VKTVPAQLAP APDAATATSA SAAPATTAAS AAASSAAASS AQ

NT Sequence

ATGCGCGTCG AACGGGTTCC ATACCGCTTA ATCACTGCCG CAACGGCTGC CGTTTTCCTG GCCGCGTGCG GGAAAAAAGA ATCGGCACCG CCGCCGCAAA CGCCGGAAGT CGGCGTCGTC ACCGTGCAGC CGCAAGCCGT GCCGGCGGTG ACCGAACTGC CGGGCCGCAC CAGTGCATTC CTCGTCGCGC AGGTGCGTGC GCGGGTCGAC GGCATCGTGC TGCGTCGTGA ATTCACCGAA GGCACCGACG TCAAGGCCGG CCAGCGTCTG TACAAGATCG ATCCGGCGCC GTACGTCGCG GCCCTGAACA GCGCGAAGGC GACGCTCGCG AAGGCGCAGG CGAACCTCGT CACGCAGAAC GCGCTGGTCG CGCGCTACAA GGTGCTGGTC GCCGCGAACG CGGTCAGCAA GCAGGACTAC GACAACGCGG TCGCCACGCA AGGGCAGGCC GCGGCGGACG TCGCGGCCGG CAAGGCGGCG GTCGACACCG CGCAGATCAA CCTCGGCTAC ACGGATGTCG TGTCGCCGAT CACCGGCCGC GTCGGCATCT CGCAAGTGAC GCCGGGCGCC TACGTGCAGG CGAGCCAGGC GACGCTGATG TCGACCGTGC AGCAGCTCGA TCCGGTGTAC GTCGACCTCA CGCAGTCGAG CCTCGAAGGG CTGAAGCTGC GTCAGGACGT GCAGAGCGGC CGTCTGAAGA CGACCGGTCC GGGCGCGGCG AAGGTGTCGT TGATTCTCGA AGACGGCAAG ACCTATTCGG AGCCGGGCAA GCTGCAGTTC TCGGACGTGA CGGTCGACCA GACCACCGGC TCGGTGACGA TCCGCGCGAT CTTCCCGAAC CCGGGCCGCG TGCTGCTGCC GGGCATGTTC GTGCGTGCAC GGATCGAGGA AGGCGTGAAC GAGAACGCGT TCCTCGTGCC GCAGATCGGC GTCACGCACG ACCAGAAGGG GCAGGCGATC GCACTCGTCG TGACCGCGGC CAACAAGGTC GAGATGCGTC CGTTGACGAC GACCGGCACG TACGGCCAGA ACTGGATCGT CGAAGGCGGT CTGCAGGCGG GCGATCACGT GATCGTGCAA GGCGTCGACA AGGTGCGCCC GGGCGCGACC GTGAAGACCG TCCCCGCGCA GCTCGCCCCG GCGCCGGACG CCGCCACCGC CACATCGGCA AGCGCCGCGC CGGCGACTAC CGCGGCCAGC GCCGCAGCGA GTTCGGCCGC CGCGTCGAGC GCGCAA

Details for BuceA.00107.g.A2.GE32143

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	42kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	94

Validated AA Sequence

MGKKXSAPPP QTPEVGVXTV QPQAVPAVTE LPGRTSAFLV AXVRAXXXXI VLRREFTEGT DVKAGQRLYK IDPAPYVAAL NSAKATLAKA QANLVTQNAL VARYKVLVAA NAVSKQDYDN AVATQGQAAA DVAAGKAAVD TAQINLGYTD VVSPITGRVG ISQVTPGAYV QASQATLMST VQQLDPVYVD LTQSSLEGLK LRQDVQSGRL KTTGPGAAKV SLILEDGKTY SEPGKLQFSD VTVDQTTGSV TIRAIFPNPG RVLLPGMFVR ARIEEGVNEN AFLVPQIGVT HDQKGQAIAL VVTAANKVEM RPLTTTGTYG QNWIVEGGLQ AGDHVIVQGV DKVRPGATVK TVPAQLAPAP DAATATSASA APATTAASAA ASSAAASSAQ

Validated NT Sequence

attgcgcgct cgacgcggcg gccgaactcg ctgcggcgct ggccgcggta gtcgccggcg cggcgcttgc cgatgtggcg gtggcggcgt ccggcgccgg ggcgagctgc gcggggacgg tcttcacggt cgcgcccggg cgcaccttgt cgacgccttg cacgatcacg tgatcgcccg cctgcagacc gccttcgacg atccagttct ggccgtacgt gccggtcgtc gtcaacggac gcatctcgac cttgttggcc gcggtcacga cgagtgcgat cgcctgcccc ttctggtcgt gcgtgacgcc gatctgcggc acgaggaacg cgttctcgtt cacgccttcc tcgatccgtg cacgcacgaa catgcccggc agcagcacgc ggcccgggtt cgggaagatc gcgcggatcg tcaccgagcc ggtggtctgg tcgaccgtca cgtccgagaa ctgcagcttg cccggctccg aataggtctt gccgtcttcg agaatcaacg acaccttcgc cgcgcccgga ccggtcgtct tcagacggcc gctctgcacg tcctgacgca gcttcagccc ttcgaggctc gactgcgtga ggtcgacgta caccggatcg agctgctgca cggtcgacat cagcgtcgcc tggctcgcct gcacgtaggc gcccggcgtc acttgcgaga tgccgacgcg gccggtgatc ggcgacacga catccgtgta gccgaggttg atctgcgcgg tgtcgaccgc cgccttgccg gccgcgacgt ccgccgcggc ctgcccttgc gtggcgaccg cgttgtcgta gtcctgcttg ctgaccgcgt tcgcggcgac cagcaccttg tagcgcgcga ccagcgcgtt ctgcgtgacg aggttcgcct gcgccttcgc gagcgtcgcc ttcgcgctgt tcagggccgc gacgtacggc gccggatcga tcttgtacag acgctggccg gccttgacgt cggtgccttc ggtgaattca cgacgcagca cgatgcngtn gnnnngngca cgcacntgcg cgacgaggaa tgcactggtg cggcccggca gttcggtcac cgccggcacg gcttgcggct gcacggtgan gacgccgact tccggcgttt gcggcggcgg tgcngattnt tttttcccca tcgaaccagg acc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGKKESAPP PQTPEVGVVT VQPQAVPAVT ELPGRTSAFL VAQVRARVDG IVLRREFTEG TDVKAGQRLY KIDPAPYVAA LNSAKATLAK AQANLVTQNA LVARYKVLVA ANAVSKQDYD NAVATQGQAA ADVAAGKAAV DTAQINLGYT DVVSPITGRV GISQVTPGAY VQASQATLMS TVQQLDPVYV DLTQSSLEGL KLRQDVQSGR LKTTGPGAAK VSLILEDGKT YSEPGKLQFS DVTVDQTTGS VTIRAIFPNP GRVLLPGMFV RARIEEGVNE NAFLVPQIGV THDQKGQAIA LVVTAANKVE MRPLTTTGTY GQNWIVEGGL QAGDHVIVQG VDKVRPGATV KTVPAQLAPA PDAATATSAS AAPATTAASA AASSAAASSA Q

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggggaaaaaa gaatcggcac cgccgccgca aacgccggaa gtcggcgtcg tcaccgtgca gccgcaagcc gtgccggcgg tgaccgaact gccgggccgc accagtgcat tcctcgtcgc gcaggtgcgt gcgcgggtcg acggcatcgt gctgcgtcgt gaattcaccg aaggcaccga cgtcaaggcc ggccagcgtc tgtacaagat cgatccggcg ccgtacgtcg cggccctgaa cagcgcgaag gcgacgctcg cgaaggcgca ggcgaacctc gtcacgcaga acgcgctggt cgcgcgctac aaggtgctgg tcgccgcgaa cgcggtcagc aagcaggact acgacaacgc ggtcgccacg caagggcagg ccgcggcgga cgtcgcggcc ggcaaggcgg cggtcgacac cgcgcagatc aacctcggct acacggatgt cgtgtcgccg atcaccggcc gcgtcggcat ctcgcaagtg acgccgggcg cctacgtgca ggcgagccag gcgacgctga tgtcgaccgt gcagcagctc gatccggtgt acgtcgacct cacgcagtcg agcctcgaag ggctgaagct gcgtcaggac gtgcagagcg gccgtctgaa gacgaccggt ccgggcgcgg cgaaggtgtc gttgattctc gaagacggca agacctattc ggagccgggc aagctgcagt tctcggacgt gacggtcgac cagaccaccg gctcggtgac gatccgcgcg atcttcccga acccgggccg cgtgctgctg ccgggcatgt tcgtgcgtgc acggatcgag gaaggcgtga acgagaacgc gttcctcgtg ccgcagatcg gcgtcacgca cgaccagaag gggcaggcga tcgcactcgt cgtgaccgcg gccaacaagg tcgagatgcg tccgttgacg acgaccggca cgtacggcca gaactggatc gtcgaaggcg gtctgcaggc gggcgatcac gtgatcgtgc aaggcgtcga caaggtgcgc ccgggcgcga ccgtgaagac cgtccccgcg cagctcgccc cggcgccgga cgccgccacc gccacatcgg caagcgccgc gccggcgact accgcggcca gcgccgcagc gagttcggcc gccgcgtcga gcgcgcaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BuceA.00107.g.A2.PS01155

PURIFICATION DATe:	7/14/2011
CONCENTRATION:	98.65mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGGAAAAAA GAATCGGCAC CGCCGCCGCA AACGCCGGAA GTCGGCGTCG TCACCGTGCA GCCGCAAGCC GTGCCGGCGG TGACCGAACT GCCGGGCCGC ACCAGTGCAT TCCTCGTCGC GCAGGTGCGT GCGCGGGTCG ACGGCATCGT GCTGCGTCGT GAATTCACCG AAGGCACCGA CGTCAAGGCC GGCCAGCGTC TGTACAAGAT CGATCCGGCG CCGTACGTCG CGGCCCTGAA CAGCGCGAAG GCGACGCTCG CGAAGGCGCA GGCGAACCTC GTCACGCAGA ACGCGCTGGT CGCGCGCTAC AAGGTGCTGG TCGCCGCGAA CGCGGTCAGC AAGCAGGACT ACGACAACGC GGTCGCCACG CAAGGGCAGG CCGCGGCGGA CGTCGCGGCC GGCAAGGCGG CGGTCGACAC CGCGCAGATC AACCTCGGCT ACACGGATGT CGTGTCGCCG ATCACCGGCC GCGTCGGCAT CTCGCAAGTG ACGCCGGGCG CCTACGTGCA GGCGAGCCAG GCGACGCTGA TGTCGACCGT GCAGCAGCTC GATCCGGTGT ACGTCGACCT CACGCAGTCG AGCCTCGAAG GGCTGAAGCT GCGTCAGGAC GTGCAGAGCG GCCGTCTGAA GACGACCGGT CCGGGCGCGG CGAAGGTGTC GTTGATTCTC GAAGACGGCA AGACCTATTC GGAGCCGGGC AAGCTGCAGT TCTCGGACGT GACGGTCGAC CAGACCACCG GCTCGGTGAC GATCCGCGCG ATCTTCCCGA ACCCGGGCCG CGTGCTGCTG CCGGGCATGT TCGTGCGTGC ACGGATCGAG GAAGGCGTGA ACGAGAACGC GTTCCTCGTG CCGCAGATCG GCGTCACGCA CGACCAGAAG GGGCAGGCGA TCGCACTCGT CGTGACCGCG GCCAACAAGG TCGAGATGCG TCCGTTGACG ACGACCGGCA CGTACGGCCA GAACTGGATC GTCGAAGGCG GTCTGCAGGC GGGCGATCAC GTGATCGTGC AAGGCGTCGA CAAGGTGCGC CCGGGCGCGA CCGTGAAGAC CGTCCCCGCG CAGCTCGCCC CGGCGCCGGA CGCCGCCACC GCCACATCGG CAAGCGCCGC GCCGGCGACT ACCGCGGCCA GCGCCGCAGC GAGTTCGGCC GCCGCGTCGA GCGCGCAATA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A