BuceA.00107.f

Efflux system transport protein

CENTER ID: BuceA.00107.f
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00107.f.A2.GE32142 Mature protein( BuceA.00107.f ) 23 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00107.f.A2.PS01147 Mature protein( BuceA.00107.f ) 23 405

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.7298
RefSeq: YP_002153973.1
UniProt: B4ENY5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYRPRQLFA ALSILAIVGT AGCNRQTGAP PGGFATQTAQ VGVLSLAPQR IVETTELSGR LSAQKVSDVR PQVSGIILKR LFVEGSDVKA GQVLYQIDPA TYQAAYDQAR GTLENARATL ASAKTKADRF TELVKINAVS KQDYDDAVAA VRADAASVTA DEAALESARV NLEYTHVRAP ISGRIGASTV TEGALVTSNQ TTALATIQAF DRMYLDVTRP STEWLRLRKA FASGRLKKTG SDSAAVTLVM EDGTEYAHPG ALQFSGVTVD ATTGSVTLRS TFPNPERELL PGMFVRARLE EGENDNAILV PQMAVTRASD GKASVYVVDA AGNARQVQVT ADNAFRDQWI VTSGLHAGDR VIVSGLQSVH AGAPVKVAAD TPQTQANAAS AAPATDTAST VSARR
NT Sequence
ATGACGTACA GGCCGCGCCA GCTGTTTGCC GCACTCTCGA TCCTTGCGAT AGTCGGAACC GCCGGCTGCA ACAGGCAAAC CGGCGCACCG CCCGGCGGCT TCGCCACGCA GACCGCCCAG GTGGGCGTGC TCTCGCTCGC GCCGCAGCGG ATCGTCGAAA CGACCGAACT TTCCGGCCGC CTTTCCGCGC AGAAGGTCTC CGACGTACGA CCGCAGGTGA GCGGCATCAT CCTCAAGCGC TTGTTCGTGG AAGGCAGTGA CGTGAAGGCG GGCCAGGTGC TCTACCAGAT CGATCCGGCC ACCTACCAGG CTGCCTACGA CCAGGCACGC GGCACGCTGG AAAACGCCCG CGCCACGCTG GCTTCGGCGA AGACGAAAGC GGACCGCTTC ACCGAACTCG TGAAGATCAA CGCCGTGAGC AAGCAGGACT ACGACGACGC CGTCGCCGCG GTTCGCGCAG ACGCCGCGAG CGTCACGGCC GACGAGGCAG CGCTCGAATC CGCGCGCGTG AACCTCGAAT ACACGCACGT GCGTGCGCCC ATCTCCGGAC GCATCGGCGC ATCGACCGTG ACGGAAGGCG CGCTCGTGAC CTCGAACCAG ACCACCGCGC TCGCCACCAT CCAGGCGTTC GACCGGATGT ACCTCGACGT CACGCGCCCC AGCACGGAAT GGCTGAGGCT TCGCAAGGCC TTCGCTTCCG GGCGCCTGAA GAAAACGGGC AGCGACAGCG CCGCCGTCAC GCTCGTGATG GAAGACGGCA CCGAATACGC CCACCCCGGC GCGCTGCAGT TCTCCGGCGT CACGGTGGAC GCCACCACCG GGTCGGTCAC GCTGCGCAGC ACGTTCCCGA ACCCCGAACG CGAATTGCTT CCCGGCATGT TCGTGCGCGC CCGCCTGGAA GAAGGCGAGA ACGACAACGC GATACTCGTG CCGCAGATGG CCGTGACGCG CGCGTCGGAC GGCAAGGCGA GCGTCTACGT CGTGGATGCG GCCGGCAACG CGCGGCAAGT GCAAGTCACG GCCGACAACG CATTCCGGGA CCAATGGATC GTGACCTCGG GCCTGCACGC CGGCGACCGC GTGATCGTGA GCGGCCTGCA GAGCGTGCAT GCGGGCGCGC CGGTCAAGGT GGCGGCCGAT ACGCCGCAGA CGCAGGCGAA CGCGGCATCC GCGGCACCCG CGACGGACAC CGCCTCGACC GTGTCGGCAC GACGC
Details for BuceA.00107.f.A2.GE32142
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 42kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 94
Validated AA Sequence
MCNXXTGAPP GGFATQTAXV GVLSLAPXRI VETTELSGRL XAQXXSDVRP QVSGIILKRL FVEGSDVKAG QVLYQIDPAT YQAAYDQARG TLENARATLX SAKTKADRFT ELVKINAVSK QDYDDAVAAV RADAASVTAD EAALESARVN LEYTHVRAPI SGRIGASTVT EGALVTSNQT TALATIQAFD RMYLDVTRPS TEWLRLRKAF ASGRLKKTGS DSAAVTLVME DGTEYAHPGA LQFSGVTVDA TTGSVTLRST FPNPERELLP GMFVRARLEE GENDNAILVP QMAVTRASDG KASVYVVDAA GNARQVQVTA DNAFRDQWIV TSGLHAGDRV IVSGLQSVHA GAPVKVAADT PQTQANAASA APATDTASTV SARR
Validated NT Sequence
agcgtcgtgc cgacacggtc gaggcggtgt ccgtcgcggg tgccgcggat gccgcgttcg cctgcgtctg cggcgtatcg gccgccacct tgaccggcgc gcccgcatgc acgctctgca ggccgctcac gatcacgcgg tcgccggcgt gcaggcccga ggtcacgatc cattggtccc ggaatgcgtt gtcggccgtg acttgcactt gccgcgcgtt gccggccgca tccacgacgt agacgctcgc cttgccgtcc gacgcgcgcg tcacggccat ctgcggcacg agtatcgcgt tgtcgttctc gccttcttcc aggcgggcgc gcacgaacat gccgggaagc aattcgcgtt cggggttcgg gaacgtgctg cgcagcgtga ccgacccggt ggtggcgtcc accgtgacgc cggagaactg cagcgcgccg gggtgggcgt attcggtgcc gtcttccatc acgagcgtga cggcggcgct gtcgctgccc gttttcttca ggcgcccgga agcgaaggcc ttgcgaagcc tcagccattc cgtgctgggg cgcgtgacgt cgaggtacat ccggtcgaac gcctggatgg tggcgagcgc ggtggtctgg ttcgaggtca cgagcgcgcc ttccgtcacg gtcgatgcgc cgatgcgtcc ggagatgggc gcacgcacgt gcgtgtattc gaggttcacg cgcgcggatt cgagcgctgc ctcgtcggcc gtgacgctcg cggcgtctgc gcgaaccgcg gcgacggcgt cgtcgtagtc ctgcttgctc acggcgttga tcttcacgag ttcggtgaag cggtccgctt tcgtcttcgc cgaanccagc gtggcgcggg cgttttccag cgtgccgcgt gcctggtcgt aggcagcctg gtaggtggcc ggatcgatct ggtagagcac ctggcccgcc ttcacgtcac tgccttccac gaacaagcgc ttgaggatga tgccgctcac ctgcggtcgt acgtcggagn cnttctgcgc nnaaaggcgg ccggaaagtt cggtcgtttc gacgatccgn tgcggcgcga gcgagagcac gcccacntgg gcggtctgcg tggcgaagcc nccgggcggt gcgccggttn ncntgttgca catngaacca ggnccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCNRQTGAP PGGFATQTAQ VGVLSLAPQR IVETTELSGR LSAQKVSDVR PQVSGIILKR LFVEGSDVKA GQVLYQIDPA TYQAAYDQAR GTLENARATL ASAKTKADRF TELVKINAVS KQDYDDAVAA VRADAASVTA DEAALESARV NLEYTHVRAP ISGRIGASTV TEGALVTSNQ TTALATIQAF DRMYLDVTRP STEWLRLRKA FASGRLKKTG SDSAAVTLVM EDGTEYAHPG ALQFSGVTVD ATTGSVTLRS TFPNPERELL PGMFVRARLE EGENDNAILV PQMAVTRASD GKASVYVVDA AGNARQVQVT ADNAFRDQWI VTSGLHAGDR VIVSGLQSVH AGAPVKVAAD TPQTQANAAS AAPATDTAST VSARR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcaacagg caaaccggcg caccgcccgg cggcttcgcc acgcagaccg cccaggtggg cgtgctctcg ctcgcgccgc agcggatcgt cgaaacgacc gaactttccg gccgcctttc cgcgcagaag gtctccgacg tacgaccgca ggtgagcggc atcatcctca agcgcttgtt cgtggaaggc agtgacgtga aggcgggcca ggtgctctac cagatcgatc cggccaccta ccaggctgcc tacgaccagg cacgcggcac gctggaaaac gcccgcgcca cgctggcttc ggcgaagacg aaagcggacc gcttcaccga actcgtgaag atcaacgccg tgagcaagca ggactacgac gacgccgtcg ccgcggttcg cgcagacgcc gcgagcgtca cggccgacga ggcagcgctc gaatccgcgc gcgtgaacct cgaatacacg cacgtgcgtg cgcccatctc cggacgcatc ggcgcatcga ccgtgacgga aggcgcgctc gtgacctcga accagaccac cgcgctcgcc accatccagg cgttcgaccg gatgtacctc gacgtcacgc gccccagcac ggaatggctg aggcttcgca aggccttcgc ttccgggcgc ctgaagaaaa cgggcagcga cagcgccgcc gtcacgctcg tgatggaaga cggcaccgaa tacgcccacc ccggcgcgct gcagttctcc ggcgtcacgg tggacgccac caccgggtcg gtcacgctgc gcagcacgtt cccgaacccc gaacgcgaat tgcttcccgg catgttcgtg cgcgcccgcc tggaagaagg cgagaacgac aacgcgatac tcgtgccgca gatggccgtg acgcgcgcgt cggacggcaa ggcgagcgtc tacgtcgtgg atgcggccgg caacgcgcgg caagtgcaag tcacggccga caacgcattc cgggaccaat ggatcgtgac ctcgggcctg cacgccggcg accgcgtgat cgtgagcggc ctgcagagcg tgcatgcggg cgcgccggtc aaggtggcgg ccgatacgcc gcagacgcag gcgaacgcgg catccgcggc acccgcgacg gacaccgcct cgaccgtgtc ggcacgacgc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for BuceA.00107.f.A2.PS01147
PURIFICATION DATe: 7/7/2011
CONCENTRATION: 47.87mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MCNXXTGAPP GGFATQTAXV GVLSLAPXRI VETTELSGRL XAQXXSDVRP QVSGIILKRL FVEGSDVKAG QVLYQIDPAT YQAAYDQARG TLENARATLX SAKTKADRFT ELVKINAVSK QDYDDAVAAV RADAASVTAD EAALESARVN LEYTHVRAPI SGRIGASTVT EGALVTSNQT TALATIQAFD RMYLDVTRPS TEWLRLRKAF ASGRLKKTGS DSAAVTLVME DGTEYAHPGA LQFSGVTVDA TTGSVTLRST FPNPERELLP GMFVRARLEE GENDNAILVP QMAVTRASDG KASVYVVDAA GNARQVQVTA DNAFRDQWIV TSGLHAGDRV IVSGLQSVHA GAPVKVAADT PQTQANAASA APATDTASTV SARR
Validated NT Sequence
agcgtcgtgc cgacacggtc gaggcggtgt ccgtcgcggg tgccgcggat gccgcgttcg cctgcgtctg cggcgtatcg gccgccacct tgaccggcgc gcccgcatgc acgctctgca ggccgctcac gatcacgcgg tcgccggcgt gcaggcccga ggtcacgatc cattggtccc ggaatgcgtt gtcggccgtg acttgcactt gccgcgcgtt gccggccgca tccacgacgt agacgctcgc cttgccgtcc gacgcgcgcg tcacggccat ctgcggcacg agtatcgcgt tgtcgttctc gccttcttcc aggcgggcgc gcacgaacat gccgggaagc aattcgcgtt cggggttcgg gaacgtgctg cgcagcgtga ccgacccggt ggtggcgtcc accgtgacgc cggagaactg cagcgcgccg gggtgggcgt attcggtgcc gtcttccatc acgagcgtga cggcggcgct gtcgctgccc gttttcttca ggcgcccgga agcgaaggcc ttgcgaagcc tcagccattc cgtgctgggg cgcgtgacgt cgaggtacat ccggtcgaac gcctggatgg tggcgagcgc ggtggtctgg ttcgaggtca cgagcgcgcc ttccgtcacg gtcgatgcgc cgatgcgtcc ggagatgggc gcacgcacgt gcgtgtattc gaggttcacg cgcgcggatt cgagcgctgc ctcgtcggcc gtgacgctcg cggcgtctgc gcgaaccgcg gcgacggcgt cgtcgtagtc ctgcttgctc acggcgttga tcttcacgag ttcggtgaag cggtccgctt tcgtcttcgc cgaanccagc gtggcgcggg cgttttccag cgtgccgcgt gcctggtcgt aggcagcctg gtaggtggcc ggatcgatct ggtagagcac ctggcccgcc ttcacgtcac tgccttccac gaacaagcgc ttgaggatga tgccgctcac ctgcggtcgt acgtcggagn cnttctgcgc nnaaaggcgg ccggaaagtt cggtcgtttc gacgatccgn tgcggcgcga gcgagagcac gcccacntgg gcggtctgcg tggcgaagcc nccgggcggt gcgccggttn ncntgttgca catngaacca ggnccc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCNRQTGAP PGGFATQTAQ VGVLSLAPQR IVETTELSGR LSAQKVSDVR PQVSGIILKR LFVEGSDVKA GQVLYQIDPA TYQAAYDQAR GTLENARATL ASAKTKADRF TELVKINAVS KQDYDDAVAA VRADAASVTA DEAALESARV NLEYTHVRAP ISGRIGASTV TEGALVTSNQ TTALATIQAF DRMYLDVTRP STEWLRLRKA FASGRLKKTG SDSAAVTLVM EDGTEYAHPG ALQFSGVTVD ATTGSVTLRS TFPNPERELL PGMFVRARLE EGENDNAILV PQMAVTRASD GKASVYVVDA AGNARQVQVT ADNAFRDQWI VTSGLHAGDR VIVSGLQSVH AGAPVKVAAD TPQTQANAAS AAPATDTAST VSARR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGCAACAGG CAAACCGGCG CACCGCCCGG CGGCTTCGCC ACGCAGACCG CCCAGGTGGG CGTGCTCTCG CTCGCGCCGC AGCGGATCGT CGAAACGACC GAACTTTCCG GCCGCCTTTC CGCGCAGAAG GTCTCCGACG TACGACCGCA GGTGAGCGGC ATCATCCTCA AGCGCTTGTT CGTGGAAGGC AGTGACGTGA AGGCGGGCCA GGTGCTCTAC CAGATCGATC CGGCCACCTA CCAGGCTGCC TACGACCAGG CACGCGGCAC GCTGGAAAAC GCCCGCGCCA CGCTGGCTTC GGCGAAGACG AAAGCGGACC GCTTCACCGA ACTCGTGAAG ATCAACGCCG TGAGCAAGCA GGACTACGAC GACGCCGTCG CCGCGGTTCG CGCAGACGCC GCGAGCGTCA CGGCCGACGA GGCAGCGCTC GAATCCGCGC GCGTGAACCT CGAATACACG CACGTGCGTG CGCCCATCTC CGGACGCATC GGCGCATCGA CCGTGACGGA AGGCGCGCTC GTGACCTCGA ACCAGACCAC CGCGCTCGCC ACCATCCAGG CGTTCGACCG GATGTACCTC GACGTCACGC GCCCCAGCAC GGAATGGCTG AGGCTTCGCA AGGCCTTCGC TTCCGGGCGC CTGAAGAAAA CGGGCAGCGA CAGCGCCGCC GTCACGCTCG TGATGGAAGA CGGCACCGAA TACGCCCACC CCGGCGCGCT GCAGTTCTCC GGCGTCACGG TGGACGCCAC CACCGGGTCG GTCACGCTGC GCAGCACGTT CCCGAACCCC GAACGCGAAT TGCTTCCCGG CATGTTCGTG CGCGCCCGCC TGGAAGAAGG CGAGAACGAC AACGCGATAC TCGTGCCGCA GATGGCCGTG ACGCGCGCGT CGGACGGCAA GGCGAGCGTC TACGTCGTGG ATGCGGCCGG CAACGCGCGG CAAGTGCAAG TCACGGCCGA CAACGCATTC CGGGACCAAT GGATCGTGAC CTCGGGCCTG CACGCCGGCG ACCGCGTGAT CGTGAGCGGC CTGCAGAGCG TGCATGCGGG CGCGCCGGTC AAGGTGGCGG CCGATACGCC GCAGACGCAG GCGAACGCGG CATCCGCGGC ACCCGCGACG GACACCGCCT CGACCGTGTC GGCACGACGC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA