BuceA.00107.d

Multidrug efflux system transport protein CeoA

CENTER ID: BuceA.00107.d
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00107.d.A2.GE32141 Mature protein( BuceA.00107.d ) 34 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00107.d.A2.PW32059 Mature protein( BuceA.00107.d ) 34 405
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_155492
PATRIC ID: fig|216591.5.peg.6362
RefSeq: YP_002235155.1
UniProt: B4EJQ1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAILRTSRSR IATAAIVTLA VVGLGTFGAM RVSANAPEKA AAPLPEVDVA TVVPQTVTDW QSYSGRLEAV EKVDVRPQVS GTIVAVNFKD GALVKKGDVL FVIDPRPYQA EVDRAAAQLA AAQARNGYAQ TDWQRAQRLI GDNAIAKRDY DEKQNAAREA NANLKAAEAA LETARINLGY TRITAPVSGR VSRAEITLGN VVSAGASAAP LTTLVSVSPI YASFDADEQT YLQYINGARS GRKVPVELGL ANETGYSRSG EIDSVDNRLD TSSGTIRVRA RFDNADGTLV PGLYARVKVG GSAPHEALLV DDAAINTDQD KKFVFVVDQQ GRVSYREVQQ GMQHGNRRVI VSGLSAGDRV VVNGTQRVRP GEQVKPHMVP MTGGDAPSAP LASTAKPAAP AKADS
NT Sequence
ATGGCCATCC TACGCACCTC CCGTTCCCGA ATCGCGACCG CGGCGATCGT CACGCTCGCC GTCGTCGGCC TCGGCACGTT CGGCGCGATG CGCGTGAGCG CGAACGCCCC CGAGAAAGCG GCGGCGCCGC TGCCCGAAGT CGACGTCGCG ACCGTCGTGC CGCAGACCGT GACCGACTGG CAAAGCTATT CGGGCCGCCT CGAGGCGGTC GAGAAAGTCG ACGTGCGCCC GCAGGTGTCG GGCACGATCG TCGCGGTGAA CTTCAAGGAC GGCGCGCTCG TGAAGAAGGG CGACGTGCTG TTCGTGATCG ACCCGCGCCC GTACCAGGCG GAAGTCGACC GCGCCGCCGC GCAGCTCGCG GCCGCGCAGG CCCGCAACGG CTACGCGCAG ACCGACTGGC AGCGCGCGCA GCGGCTGATC GGCGACAACG CGATCGCGAA GCGCGACTAC GACGAGAAGC AGAACGCGGC GCGCGAAGCG AACGCGAACC TGAAGGCCGC CGAAGCCGCG CTGGAAACGG CGCGCATCAA TCTCGGCTAT ACGCGCATCA CCGCGCCGGT GTCGGGCCGC GTGTCGCGCG CGGAAATCAC GCTCGGCAAC GTCGTGTCGG CCGGCGCGTC GGCCGCGCCG CTGACGACGC TGGTATCGGT GTCGCCGATC TACGCGTCGT TCGACGCCGA CGAGCAGACC TACCTGCAAT ACATCAACGG CGCGCGCAGC GGCCGCAAGG TGCCGGTCGA GCTCGGCCTC GCGAACGAAA CCGGCTACTC GCGCAGCGGC GAGATCGATT CGGTCGACAA CCGGCTCGAC ACGTCGTCCG GCACGATCCG CGTGCGCGCC CGCTTCGACA ACGCGGACGG CACCCTGGTC CCGGGCCTCT ACGCACGCGT GAAGGTGGGC GGCAGCGCGC CGCACGAGGC GCTGCTCGTC GACGACGCGG CGATCAACAC CGACCAGGAC AAGAAGTTCG TGTTCGTCGT CGACCAGCAG GGCCGCGTGT CGTATCGCGA AGTGCAGCAA GGGATGCAGC ACGGCAACCG GCGCGTGATC GTGAGCGGGC TGTCGGCCGG CGACCGCGTG GTCGTGAACG GCACGCAGCG CGTGCGTCCG GGCGAGCAGG TGAAGCCGCA CATGGTCCCG ATGACGGGCG GCGATGCGCC GTCCGCGCCG CTCGCGAGCA CCGCGAAGCC GGCCGCACCG GCGAAGGCGG ATTCG
Details for BuceA.00107.d.A2.GE32141
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MANAPEKAAA PLPEVDVATV VPQTVTDWQS YSGRLEAVEK VDVRPQVSGT IVAVNFKDGA LVKKGDVLFV IDPRPYQAEV DRAAAQLAAA QARNGYAQTD WQRAQRLIGD NAIAKRDYDE KQNAAREANA NLKAAEAALE TARINLGYTR ITAPVSGRVS RAEITLGNVV SAGASAAPLT TLVSVSPIYA SFDADEQTYL QYINGARSGR KVPVELGLAN ETGYSRSGEI DSVDNRLDTS SGTIRVRARF DNADGTLVPG LYARVKVGGS APHEALLVDD AAINTDQDKK FVFVVDQQGR VSYREVQQGM QHGNRRVIVS GLSAGDRVVV NGTQRVRPGE QVKPHMVPMT GGDAPSAPLA STA
Validated NT Sequence
tcgcggtgct cgcgagcggc gcggacggcg catcgccgcc cgtcatcggg accatgtgcg gcttcacctg ctcgcccgga cgcacgcgct gcgtgccgtt cacgaccacg cggtcgccgg ccgacagccc gctcacgatc acgcgccggt tgccgtgctg catcccttgc tgcacttcgc gatacgacac gcggccctgc tggtcgacga cgaacacgaa cttcttgtcc tggtcggtgt tgatcgccgc gtcgtcgacg agcagcgcct cgtgcggcgc gctgccgccc accttcacgc gtgcgtagag gcccgggacc agggtgccgt ccgcgttgtc gaagcgggcg cgcacgcgga tcgtgccgga cgacgtgtcg agccggttgt cgaccgaatc gatctcgccg ctgcgcgagt agccggtttc gttcgcgagg ccgagctcga ccggcacctt gcggccgctg cgcgcgccgt tgatgtattg caggtaggtc tgctcgtcgg cgtcgaacga cgcgtagatc ggcgacaccg ataccagcgt cgtcagcggc gcggccgacg cgccggccga cacgacgttg ccgagcgtga tttccgcgcg cgacacgcgg cccgacaccg gcgcggtgat gcgcgtatag ccgagattga tgcgcgccgt ttccagcgcg gcttcggcgg ccttcaggtt cgcgttcgct tcgcgcgccg cgttctgctt ctcgtcgtag tcgcgcttcg cgatcgcgtt gtcgccgatc agncgctgcg cgcgctgcca gtcggtctgc gcgtagccgt tgcgggcctg cgcggccgcg agctgcgcgg cggcgcggtc gacttccgcc tggtacgggc gcgggtcgat cacgaacagc acgtcgccct tcttcacgag cgcgccgtcc ttgaagttca ccgcgacgat cgtgcccgac acctgcgggc gcacgtcgac tttctcgacc gcctcgaggc ggcccgaata gctttgccag tcggtcacgg tctgcggcac gacggtcgcg acgtcgactt cgggcagcgg cgccgccgct ttctcggggg cgttcgccat cgaaccagga cccngggtct gagct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMANAPEKAA APLPEVDVAT VVPQTVTDWQ SYSGRLEAVE KVDVRPQVSG TIVAVNFKDG ALVKKGDVLF VIDPRPYQAE VDRAAAQLAA AQARNGYAQT DWQRAQRLIG DNAIAKRDYD EKQNAAREAN ANLKAAEAAL ETARINLGYT RITAPVSGRV SRAEITLGNV VSAGASAAPL TTLVSVSPIY ASFDADEQTY LQYINGARSG RKVPVELGLA NETGYSRSGE IDSVDNRLDT SSGTIRVRAR FDNADGTLVP GLYARVKVGG SAPHEALLVD DAAINTDQDK KFVFVVDQQG RVSYREVQQG MQHGNRRVIV SGLSAGDRVV VNGTQRVRPG EQVKPHMVPM TGGDAPSAPL ASTAKPAAPA KADS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgaacgcc cccgagaaag cggcggcgcc gctgcccgaa gtcgacgtcg cgaccgtcgt gccgcagacc gtgaccgact ggcaaagcta ttcgggccgc ctcgaggcgg tcgagaaagt cgacgtgcgc ccgcaggtgt cgggcacgat cgtcgcggtg aacttcaagg acggcgcgct cgtgaagaag ggcgacgtgc tgttcgtgat cgacccgcgc ccgtaccagg cggaagtcga ccgcgccgcc gcgcagctcg cggccgcgca ggcccgcaac ggctacgcgc agaccgactg gcagcgcgcg cagcggctga tcggcgacaa cgcgatcgcg aagcgcgact acgacgagaa gcagaacgcg gcgcgcgaag cgaacgcgaa cctgaaggcc gccgaagccg cgctggaaac ggcgcgcatc aatctcggct atacgcgcat caccgcgccg gtgtcgggcc gcgtgtcgcg cgcggaaatc acgctcggca acgtcgtgtc ggccggcgcg tcggccgcgc cgctgacgac gctggtatcg gtgtcgccga tctacgcgtc gttcgacgcc gacgagcaga cctacctgca atacatcaac ggcgcgcgca gcggccgcaa ggtgccggtc gagctcggcc tcgcgaacga aaccggctac tcgcgcagcg gcgagatcga ttcggtcgac aaccggctcg acacgtcgtc cggcacgatc cgcgtgcgcg cccgcttcga caacgcggac ggcaccctgg tcccgggcct ctacgcacgc gtgaaggtgg gcggcagcgc gccgcacgag gcgctgctcg tcgacgacgc ggcgatcaac accgaccagg acaagaagtt cgtgttcgtc gtcgaccagc agggccgcgt gtcgtatcgc gaagtgcagc aagggatgca gcacggcaac cggcgcgtga tcgtgagcgg gctgtcggcc ggcgaccgcg tggtcgtgaa cggcacgcag cgcgtgcgtc cgggcgagca ggtgaagccg cacatggtcc cgatgacggg cggcgatgcg ccgtccgcgc cgctcgcgag caccgcgaag ccggccgcac cggcgaaggc ggattcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BuceA.00107.d.A2.PW32059
PURIFICATION DATe: 5/30/2011
CONCENTRATION: 19.75mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 24
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MANAPEKAAA PLPEVDVATV VPQTVTDWQS YSGRLEAVEK VDVRPQVSGT IVAVNFKDGA LVKKGDVLFV IDPRPYQAEV DRAAAQLAAA QARNGYAQTD WQRAQRLIGD NAIAKRDYDE KQNAAREANA NLKAAEAALE TARINLGYTR ITAPVSGRVS RAEITLGNVV SAGASAAPLT TLVSVSPIYA SFDADEQTYL QYINGARSGR KVPVELGLAN ETGYSRSGEI DSVDNRLDTS SGTIRVRARF DNADGTLVPG LYARVKVGGS APHEALLVDD AAINTDQDKK FVFVVDQQGR VSYREVQQGM QHGNRRVIVS GLSAGDRVVV NGTQRVRPGE QVKPHMVPMT GGDAPSAPLA STA
Validated NT Sequence
tcgcggtgct cgcgagcggc gcggacggcg catcgccgcc cgtcatcggg accatgtgcg gcttcacctg ctcgcccgga cgcacgcgct gcgtgccgtt cacgaccacg cggtcgccgg ccgacagccc gctcacgatc acgcgccggt tgccgtgctg catcccttgc tgcacttcgc gatacgacac gcggccctgc tggtcgacga cgaacacgaa cttcttgtcc tggtcggtgt tgatcgccgc gtcgtcgacg agcagcgcct cgtgcggcgc gctgccgccc accttcacgc gtgcgtagag gcccgggacc agggtgccgt ccgcgttgtc gaagcgggcg cgcacgcgga tcgtgccgga cgacgtgtcg agccggttgt cgaccgaatc gatctcgccg ctgcgcgagt agccggtttc gttcgcgagg ccgagctcga ccggcacctt gcggccgctg cgcgcgccgt tgatgtattg caggtaggtc tgctcgtcgg cgtcgaacga cgcgtagatc ggcgacaccg ataccagcgt cgtcagcggc gcggccgacg cgccggccga cacgacgttg ccgagcgtga tttccgcgcg cgacacgcgg cccgacaccg gcgcggtgat gcgcgtatag ccgagattga tgcgcgccgt ttccagcgcg gcttcggcgg ccttcaggtt cgcgttcgct tcgcgcgccg cgttctgctt ctcgtcgtag tcgcgcttcg cgatcgcgtt gtcgccgatc agncgctgcg cgcgctgcca gtcggtctgc gcgtagccgt tgcgggcctg cgcggccgcg agctgcgcgg cggcgcggtc gacttccgcc tggtacgggc gcgggtcgat cacgaacagc acgtcgccct tcttcacgag cgcgccgtcc ttgaagttca ccgcgacgat cgtgcccgac acctgcgggc gcacgtcgac tttctcgacc gcctcgaggc ggcccgaata gctttgccag tcggtcacgg tctgcggcac gacggtcgcg acgtcgactt cgggcagcgg cgccgccgct ttctcggggg cgttcgccat cgaaccagga cccngggtct gagct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMANAPEKAA APLPEVDVAT VVPQTVTDWQ SYSGRLEAVE KVDVRPQVSG TIVAVNFKDG ALVKKGDVLF VIDPRPYQAE VDRAAAQLAA AQARNGYAQT DWQRAQRLIG DNAIAKRDYD EKQNAAREAN ANLKAAEAAL ETARINLGYT RITAPVSGRV SRAEITLGNV VSAGASAAPL TTLVSVSPIY ASFDADEQTY LQYINGARSG RKVPVELGLA NETGYSRSGE IDSVDNRLDT SSGTIRVRAR FDNADGTLVP GLYARVKVGG SAPHEALLVD DAAINTDQDK KFVFVVDQQG RVSYREVQQG MQHGNRRVIV SGLSAGDRVV VNGTQRVRPG EQVKPHMVPM TGGDAPSAPL ASTAKPAAPA KADS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGAACGCC CCCGAGAAAG CGGCGGCGCC GCTGCCCGAA GTCGACGTCG CGACCGTCGT GCCGCAGACC GTGACCGACT GGCAAAGCTA TTCGGGCCGC CTCGAGGCGG TCGAGAAAGT CGACGTGCGC CCGCAGGTGT CGGGCACGAT CGTCGCGGTG AACTTCAAGG ACGGCGCGCT CGTGAAGAAG GGCGACGTGC TGTTCGTGAT CGACCCGCGC CCGTACCAGG CGGAAGTCGA CCGCGCCGCC GCGCAGCTCG CGGCCGCGCA GGCCCGCAAC GGCTACGCGC AGACCGACTG GCAGCGCGCG CAGCGGCTGA TCGGCGACAA CGCGATCGCG AAGCGCGACT ACGACGAGAA GCAGAACGCG GCGCGCGAAG CGAACGCGAA CCTGAAGGCC GCCGAAGCCG CGCTGGAAAC GGCGCGCATC AATCTCGGCT ATACGCGCAT CACCGCGCCG GTGTCGGGCC GCGTGTCGCG CGCGGAAATC ACGCTCGGCA ACGTCGTGTC GGCCGGCGCG TCGGCCGCGC CGCTGACGAC GCTGGTATCG GTGTCGCCGA TCTACGCGTC GTTCGACGCC GACGAGCAGA CCTACCTGCA ATACATCAAC GGCGCGCGCA GCGGCCGCAA GGTGCCGGTC GAGCTCGGCC TCGCGAACGA AACCGGCTAC TCGCGCAGCG GCGAGATCGA TTCGGTCGAC AACCGGCTCG ACACGTCGTC CGGCACGATC CGCGTGCGCG CCCGCTTCGA CAACGCGGAC GGCACCCTGG TCCCGGGCCT CTACGCACGC GTGAAGGTGG GCGGCAGCGC GCCGCACGAG GCGCTGCTCG TCGACGACGC GGCGATCAAC ACCGACCAGG ACAAGAAGTT CGTGTTCGTC GTCGACCAGC AGGGCCGCGT GTCGTATCGC GAAGTGCAGC AAGGGATGCA GCACGGCAAC CGGCGCGTGA TCGTGAGCGG GCTGTCGGCC GGCGACCGCG TGGTCGTGAA CGGCACGCAG CGCGTGCGTC CGGGCGAGCA GGTGAAGCCG CACATGGTCC CGATGACGGG CGGCGATGCG CCGTCCGCGC CGCTCGCGAG CACCGCGAAG CCGGCCGCAC CGGCGAAGGC GGATTCGTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA