BuceA.00107.c

Multidrug efflux system transport protein

CENTER ID: BuceA.00107.c
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00107.c.A2.GE32137 Mature protein( BuceA.00107.c ) 39 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00107.c.A2.PS01121 Mature protein( BuceA.00107.c ) 39 386

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4646
RefSeq: YP_002233545.1
UniProt: B4EF72

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNQKRCCYG AVCALVAAML AGCGPSEQPA AAPATPVAAM TVAATRVDVT EDLPGRVAAV RVAEIRPQVS GIVQRRLFEQ GTEVRVGQPL FQINPAPFKA EMDTAAASLQ RAQAALERAK VQTARFKPLV EADAISRQMY DDAVSQRDQT AADVAQARAT LARRQLDLKF ATVEAPIAGR IDQALVTEGA LVSSSDSQPM ARVQQIDQVY VDVRQPAASL DALRGALAAQ PQASDSNGLP VDVLRDDDTR YDVKGRMLFS GVNVDPGTGD VLLRVLVDNP KRQLLPGMYV RARIPRAHYA NAVMVPQQAI VRAGGKPQVW VLDAKGTAHL KAVEVGELTN RSYRIKSGLQ AGQKIVVEGM ERLTDGAPAA ATDWKSPDAA AIASAH
NT Sequence
ATGAAGAACC AAAAACGATG CTGCTACGGC GCCGTATGCG CGCTGGTGGC GGCAATGCTG GCGGGTTGCG GGCCGTCCGA ACAGCCGGCC GCCGCGCCGG CCACCCCGGT GGCGGCCATG ACCGTGGCCG CCACGCGCGT CGACGTGACG GAGGACCTGC CGGGCCGCGT CGCGGCCGTG CGGGTCGCCG AGATCCGGCC GCAGGTGAGC GGCATCGTGC AACGCCGGCT GTTCGAACAA GGCACCGAAG TGCGCGTGGG CCAGCCGCTG TTCCAGATCA ATCCGGCGCC GTTCAAGGCC GAGATGGACA CGGCCGCCGC GTCGCTGCAG CGCGCGCAGG CGGCGCTCGA ACGCGCGAAG GTGCAGACCG CGCGCTTCAA GCCGCTCGTC GAGGCCGATG CGATCAGCCG CCAGATGTAC GACGACGCCG TCTCGCAACG CGACCAGACC GCGGCCGACG TCGCGCAGGC CCGCGCGACG CTCGCGCGCC GCCAGCTCGA CCTGAAGTTC GCGACGGTGG AAGCGCCGAT CGCCGGCCGC ATCGACCAGG CGCTCGTGAC CGAAGGCGCG CTGGTATCGA GCAGCGACAG CCAACCGATG GCGCGCGTCC AGCAGATCGA CCAGGTCTAC GTGGACGTGC GTCAACCGGC CGCGTCGCTC GACGCGCTGC GCGGCGCGCT TGCCGCTCAA CCGCAAGCGT CGGACAGCAA CGGCCTGCCG GTCGACGTGC TGCGCGACGA CGACACGCGC TACGACGTCA AGGGCCGCAT GCTGTTCTCG GGCGTCAACG TCGATCCGGG CACCGGCGAC GTGCTGCTGC GCGTGCTGGT CGACAACCCG AAACGACAGC TGCTGCCGGG CATGTACGTG CGTGCCCGCA TTCCGCGCGC GCACTACGCC AACGCCGTGA TGGTGCCGCA ACAGGCGATC GTGCGCGCCG GCGGCAAGCC GCAGGTGTGG GTGCTCGACG CAAAGGGTAC GGCTCACCTG AAGGCGGTCG AGGTCGGCGA ACTGACGAAC CGCAGCTATC GCATCAAGTC GGGCCTGCAG GCCGGCCAGA AGATCGTCGT CGAAGGCATG GAACGCCTGA CCGACGGCGC GCCGGCCGCC GCAACCGACT GGAAGTCGCC TGACGCGGCC GCCATCGCGT CCGCGCAC
Details for BuceA.00107.c.A2.GE32137
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MAMTVAATRV DVTEDLPGRV AAVRVAEIRP QVSGIVQRRL FEQGTEVRVG QPLFQINPAP FKAEMDTAAA SLQRAQAALE RAKVQTARFK PLVEADAISR QMYDDAVSQR DQTAADVAQA RATLARRQLD LKFATVEAPI AGRIDQALVT EGALVSSSDS QPMARVQQID QVYVDVRQPA ASLDALRGAL AAQPQASDSN GLPVDVLRDD DTRYDVKGRM LFSGVNVDPG TGDVLLRVLV DNPKRQLLPG MYVRARIPRA HYANAVMVPQ QAIVRAGGKP QVWVLDAKGT AHLKAVEVGE LTNRSYRIKS GLQAGQKIVV EGMERLTDGA PAAATDWKSP DAA
Validated NT Sequence
ggcggccgcg tcaggcgact tccagtcggt tgcggcggcc ggcgcgccgt cggtcaggcg ttccatgcct tcgacgacga tcttctggcc ggcctgcagg cccgacttga tgcgatagct gcggttcgtc agttcgccga cctcgaccgc cttcaggtga gccgtaccct ttgcgtcgag cacccacacc tgcggcttgc cgccggcgcg cacgatcgcc tgttgcggca ccatcacggc gttggcgtag tgcgcgcgcg gaatgcgggc acgcacgtac atgcccggca gcagctgtcg tttcgggttg tcgaccagca cgcgcagcag cacgtcgccg gtgcccggat cgacgttgac gcccgagaac agcatgcggc ccttgacgtc gtagcgcgtg tcgtcgtcgc gcagcacgtc gaccggcagg ccgttgctgt ccgacgcttg cggttgagcg gcaagcgcgc cgcgcagcgc gtcgagcgac gcggccggtt gacgcacgtc cacgtagacc tggtcgatct gctggacgcg cgccatcggt tggctgtcgc tgctcgatac cagcgcgcct tcggtcacga gcgcctggtc gatgcggccg gcgatcggcg cttccaccgt cgcgaacttc aggtcgagct ggcggcgcgc gagcgtcgcg cgggcctgcg cgacgtcggc cgcggtctgg tcgcgttgcg agacggcgtc gtcgtacatc tggcggctga tcgcatcggc ctcgacgagc ggcttgaagc gcgcggtctg caccttcgcg cgttcgagcg ccgcctgcgc gcgctgcagc gacgcggcgg ccgtgtccat ctcggccttg aacggcgccg gattgatctg gaacagcggc tggcccacgc gcacttcggt gccttgttcg aacagccggc gttgcacgat gccgctcacc tgcggccgga tctcggcgac ccgcacggcc gcgacgcggc ccggcaggtc ctccgtcacg tcgacgcgcg tggcggccac ggtcatggcc atcgaaccag gaccctgggt ctgagcttcc aggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAMTVAATR VDVTEDLPGR VAAVRVAEIR PQVSGIVQRR LFEQGTEVRV GQPLFQINPA PFKAEMDTAA ASLQRAQAAL ERAKVQTARF KPLVEADAIS RQMYDDAVSQ RDQTAADVAQ ARATLARRQL DLKFATVEAP IAGRIDQALV TEGALVSSSD SQPMARVQQI DQVYVDVRQP AASLDALRGA LAAQPQASDS NGLPVDVLRD DDTRYDVKGR MLFSGVNVDP GTGDVLLRVL VDNPKRQLLP GMYVRARIPR AHYANAVMVP QQAIVRAGGK PQVWVLDAKG TAHLKAVEVG ELTNRSYRIK SGLQAGQKIV VEGMERLTDG APAAATDWKS PDAAAIASAH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccatgacc gtggccgcca cgcgcgtcga cgtgacggag gacctgccgg gccgcgtcgc ggccgtgcgg gtcgccgaga tccggccgca ggtgagcggc atcgtgcaac gccggctgtt cgaacaaggc accgaagtgc gcgtgggcca gccgctgttc cagatcaatc cggcgccgtt caaggccgag atggacacgg ccgccgcgtc gctgcagcgc gcgcaggcgg cgctcgaacg cgcgaaggtg cagaccgcgc gcttcaagcc gctcgtcgag gccgatgcga tcagccgcca gatgtacgac gacgccgtct cgcaacgcga ccagaccgcg gccgacgtcg cgcaggcccg cgcgacgctc gcgcgccgcc agctcgacct gaagttcgcg acggtggaag cgccgatcgc cggccgcatc gaccaggcgc tcgtgaccga aggcgcgctg gtatcgagca gcgacagcca accgatggcg cgcgtccagc agatcgacca ggtctacgtg gacgtgcgtc aaccggccgc gtcgctcgac gcgctgcgcg gcgcgcttgc cgctcaaccg caagcgtcgg acagcaacgg cctgccggtc gacgtgctgc gcgacgacga cacgcgctac gacgtcaagg gccgcatgct gttctcgggc gtcaacgtcg atccgggcac cggcgacgtg ctgctgcgcg tgctggtcga caacccgaaa cgacagctgc tgccgggcat gtacgtgcgt gcccgcattc cgcgcgcgca ctacgccaac gccgtgatgg tgccgcaaca ggcgatcgtg cgcgccggcg gcaagccgca ggtgtgggtg ctcgacgcaa agggtacggc tcacctgaag gcggtcgagg tcggcgaact gacgaaccgc agctatcgca tcaagtcggg cctgcaggcc ggccagaaga tcgtcgtcga aggcatggaa cgcctgaccg acggcgcgcc ggccgccgca accgactgga agtcgcctga cgcggccgcc atcgcgtccg cgcactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BuceA.00107.c.A2.PS01121
PURIFICATION DATe: 6/15/2011
CONCENTRATION: 49.87mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MAMTVAATRV DVTEDLPGRV AAVRVAEIRP QVSGIVQRRL FEQGTEVRVG QPLFQINPAP FKAEMDTAAA SLQRAQAALE RAKVQTARFK PLVEADAISR QMYDDAVSQR DQTAADVAQA RATLARRQLD LKFATVEAPI AGRIDQALVT EGALVSSSDS QPMARVQQID QVYVDVRQPA ASLDALRGAL AAQPQASDSN GLPVDVLRDD DTRYDVKGRM LFSGVNVDPG TGDVLLRVLV DNPKRQLLPG MYVRARIPRA HYANAVMVPQ QAIVRAGGKP QVWVLDAKGT AHLKAVEVGE LTNRSYRIKS GLQAGQKIVV EGMERLTDGA PAAATDWKSP DAA
Validated NT Sequence
ggcggccgcg tcaggcgact tccagtcggt tgcggcggcc ggcgcgccgt cggtcaggcg ttccatgcct tcgacgacga tcttctggcc ggcctgcagg cccgacttga tgcgatagct gcggttcgtc agttcgccga cctcgaccgc cttcaggtga gccgtaccct ttgcgtcgag cacccacacc tgcggcttgc cgccggcgcg cacgatcgcc tgttgcggca ccatcacggc gttggcgtag tgcgcgcgcg gaatgcgggc acgcacgtac atgcccggca gcagctgtcg tttcgggttg tcgaccagca cgcgcagcag cacgtcgccg gtgcccggat cgacgttgac gcccgagaac agcatgcggc ccttgacgtc gtagcgcgtg tcgtcgtcgc gcagcacgtc gaccggcagg ccgttgctgt ccgacgcttg cggttgagcg gcaagcgcgc cgcgcagcgc gtcgagcgac gcggccggtt gacgcacgtc cacgtagacc tggtcgatct gctggacgcg cgccatcggt tggctgtcgc tgctcgatac cagcgcgcct tcggtcacga gcgcctggtc gatgcggccg gcgatcggcg cttccaccgt cgcgaacttc aggtcgagct ggcggcgcgc gagcgtcgcg cgggcctgcg cgacgtcggc cgcggtctgg tcgcgttgcg agacggcgtc gtcgtacatc tggcggctga tcgcatcggc ctcgacgagc ggcttgaagc gcgcggtctg caccttcgcg cgttcgagcg ccgcctgcgc gcgctgcagc gacgcggcgg ccgtgtccat ctcggccttg aacggcgccg gattgatctg gaacagcggc tggcccacgc gcacttcggt gccttgttcg aacagccggc gttgcacgat gccgctcacc tgcggccgga tctcggcgac ccgcacggcc gcgacgcggc ccggcaggtc ctccgtcacg tcgacgcgcg tggcggccac ggtcatggcc atcgaaccag gaccctgggt ctgagcttcc aggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAMTVAATR VDVTEDLPGR VAAVRVAEIR PQVSGIVQRR LFEQGTEVRV GQPLFQINPA PFKAEMDTAA ASLQRAQAAL ERAKVQTARF KPLVEADAIS RQMYDDAVSQ RDQTAADVAQ ARATLARRQL DLKFATVEAP IAGRIDQALV TEGALVSSSD SQPMARVQQI DQVYVDVRQP AASLDALRGA LAAQPQASDS NGLPVDVLRD DDTRYDVKGR MLFSGVNVDP GTGDVLLRVL VDNPKRQLLP GMYVRARIPR AHYANAVMVP QQAIVRAGGK PQVWVLDAKG TAHLKAVEVG ELTNRSYRIK SGLQAGQKIV VEGMERLTDG APAAATDWKS PDAAAIASAH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCATGACC GTGGCCGCCA CGCGCGTCGA CGTGACGGAG GACCTGCCGG GCCGCGTCGC GGCCGTGCGG GTCGCCGAGA TCCGGCCGCA GGTGAGCGGC ATCGTGCAAC GCCGGCTGTT CGAACAAGGC ACCGAAGTGC GCGTGGGCCA GCCGCTGTTC CAGATCAATC CGGCGCCGTT CAAGGCCGAG ATGGACACGG CCGCCGCGTC GCTGCAGCGC GCGCAGGCGG CGCTCGAACG CGCGAAGGTG CAGACCGCGC GCTTCAAGCC GCTCGTCGAG GCCGATGCGA TCAGCCGCCA GATGTACGAC GACGCCGTCT CGCAACGCGA CCAGACCGCG GCCGACGTCG CGCAGGCCCG CGCGACGCTC GCGCGCCGCC AGCTCGACCT GAAGTTCGCG ACGGTGGAAG CGCCGATCGC CGGCCGCATC GACCAGGCGC TCGTGACCGA AGGCGCGCTG GTATCGAGCA GCGACAGCCA ACCGATGGCG CGCGTCCAGC AGATCGACCA GGTCTACGTG GACGTGCGTC AACCGGCCGC GTCGCTCGAC GCGCTGCGCG GCGCGCTTGC CGCTCAACCG CAAGCGTCGG ACAGCAACGG CCTGCCGGTC GACGTGCTGC GCGACGACGA CACGCGCTAC GACGTCAAGG GCCGCATGCT GTTCTCGGGC GTCAACGTCG ATCCGGGCAC CGGCGACGTG CTGCTGCGCG TGCTGGTCGA CAACCCGAAA CGACAGCTGC TGCCGGGCAT GTACGTGCGT GCCCGCATTC CGCGCGCGCA CTACGCCAAC GCCGTGATGG TGCCGCAACA GGCGATCGTG CGCGCCGGCG GCAAGCCGCA GGTGTGGGTG CTCGACGCAA AGGGTACGGC TCACCTGAAG GCGGTCGAGG TCGGCGAACT GACGAACCGC AGCTATCGCA TCAAGTCGGG CCTGCAGGCC GGCCAGAAGA TCGTCGTCGA AGGCATGGAA CGCCTGACCG ACGGCGCGCC GGCCGCCGCA ACCGACTGGA AGTCGCCTGA CGCGGCCGCC ATCGCGTCCG CGCACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA