BuceA.00102.a

2-dehydro-3-deoxyphosphooctonate aldolase

CENTER ID:	BuceA.00102.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00102.a.A1.GE32134	Full length( BuceA.00102.a )		1	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

3TML

DEPOSITED: 8/31/2011

DETERMINATION: XRay

Diffraction Image

CLONE: BuceA.00102.a.A1.GE32134

PROTEIN: BuceA.00102.a.A1.PS01142

Publications by SSGCID

Combining functional and structural genomics to sample the essential Burkholderia structome.

Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC

PLoS ONE - 2012

volume 8, issue 1, pages e53851

PMID: 23382856; PMCID: PMC3561365

PDB: 3D63, 3DAH, 3EIZ, 3EJ2, 3EK2, 3EZO, 3F0F, 3FTP, 3GK0, 3GK3, 3GVF, 3GWA, 3GWE, 3IML, 3SZ8, 3T4C, 3TML, 3TMQ, 3TXY, 3U7J, 3UE9, 3UK1, 3UK2, 3UND, 3UPT, 3URR, 3UW1, 3UW2, 3UW3, 3V2I, 3V7N, 3V8H, 3V9O, 3V9P, 3VAV, 4DDO, 4DFE, 4DHE, 4DHK, 4DUT, 4DZ4, 4E4T, 4E51, 4EFI, 4EG0, 4EGJ, 4EK2, 4EQY, 4EWG, 4EX5, 4EXQ, 4F2G, 4F32, 4F3N, 4F3Y, 4F4H, 4F7D, 4FK8, 4FRY, 4G1K, 4G67, 4G6Z, 4GHK, 4H3Y, 4H3Z, 4H4G

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130461
PATRIC ID:	fig\|216591.5.peg.2269
RefSeq:	YP_002231307.1
UniProt:	B4EDA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLCDFEVGL DQPFFLIAGT CVVESEQMTI DTAGRLKEIC EKLNVPFIYK SSYDKANRSS GKSFRGLGMD EGLRILSEVK RQLGLPVLTD VHSIDEIEQV ASVVDVLQTP AFLCRQTDFI HACARSGKPV NIKKGQFLAP HDMKNVIDKA RDAAREAGLS EDRFMACERG VSFGYNNLVS DMRSLAIMRE TNAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLARAAVAT GVAGLFMETH PNPAEAKSDG PNAVPLNRMG ALLETLVTLD QAVKRNPFLE NDFN

NT Sequence

ATGAAGCTGT GCGATTTCGA GGTCGGTCTC GACCAGCCTT TCTTCCTGAT CGCGGGTACC TGCGTCGTCG AATCGGAGCA AATGACGATC GACACGGCGG GCCGCCTGAA GGAGATCTGC GAGAAGCTGA ACGTTCCGTT CATCTACAAG TCGTCCTACG ACAAGGCAAA CCGCAGCTCG GGCAAGTCGT TTCGCGGCCT CGGAATGGAC GAGGGCCTGC GGATCCTGTC CGAGGTGAAG CGCCAGCTCG GCCTGCCGGT GCTGACCGAC GTGCATTCGA TCGACGAGAT CGAGCAGGTC GCGTCGGTCG TCGACGTGCT GCAGACGCCG GCGTTCCTGT GCCGCCAGAC CGACTTCATC CATGCGTGCG CGCGCTCGGG CAAGCCGGTC AACATCAAGA AGGGCCAGTT CCTCGCGCCG CACGACATGA AGAACGTGAT CGACAAGGCG CGCGACGCCG CACGTGAAGC CGGTCTGTCG GAAGACCGTT TCATGGCGTG CGAGCGCGGC GTCTCGTTCG GCTACAACAA TCTCGTGTCG GACATGCGTT CGCTCGCGAT CATGCGCGAG ACGAACGCGC CGGTCGTGTT CGATGCGACC CACTCGGTGC AATTGCCGGG CGGGCAGGGC ACGAGCTCGG GCGGCCAACG CGAATTCGTG CCGGTGCTCG CCCGTGCGGC GGTCGCGACG GGCGTCGCGG GCCTCTTCAT GGAAACGCAT CCGAATCCGG CCGAGGCGAA GTCGGACGGC CCGAATGCGG TGCCGCTGAA CCGGATGGGC GCGTTGCTGG AGACCCTCGT CACGCTCGAC CAGGCGGTGA AGCGCAATCC GTTCCTGGAA AACGATTTCA AT

Details for BuceA.00102.a.A1.GE32134

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ttattaattg aaatcgtttt ccaggaacgg attgcgcttc accgcctggt cgagcgtgac gagggtctcc agcaacgcgc ccatccggtt cagcggcacc gcattcgggc cgtccgactt cgcctcggcc ggattcggat gcgtttccat gaagaggccc gcgacgcccg tcgcgaccgc cgcacgggcg agcaccggca cgaattcgcg ttggccgccc gagctcgtgc cctgcccgcc cggcaattgc accgagtggg tcgcatcgaa cacgaccggc gcgttcgtct cgcgcatgat cgcgagcgaa cgcatgtccg acacgagatt gttgtagccg aacgagacgc cgcgctcgca cgccatgaaa cggtcttccg acagaccggc ttcacgtgcg gcgtcgcgcg ccttgtcgat cacgttcttc atgtcgtgcg gcgcgaggaa ctggcccttc ttgatgttga ccggcttgcc cgagcgcgcg cacgcatgga tgaagtcggt ctggcggcac aggaacgccg gcgtctgcag cacgtcgacg accgacgcga cctgctcgat ctcgtcgatc gaatgcacgt cggtcagcac cggcaggccg agctggcgct tcacctcgga caggatccgc aggccctcgt ccattccgag gccgcgaaac gacttgcccg agctgcggtt tgccttgtcg taggacgact tgtagatgaa cggaacgttc agcttctcgc agatctcctt caggcggccc gccgtgtcga tcgtcatttg ctccgattcg acgacgcagg tacccgcgat caggaagaaa ggctggtcga gaccgacctc gaaatcgcac agcttcatcg aaccaggacc ctgggtctga gcttccaggg tacc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKLCDFEVG LDQPFFLIAG TCVVESEQMT IDTAGRLKEI CEKLNVPFIY KSSYDKANRS SGKSFRGLGM DEGLRILSEV KRQLGLPVLT DVHSIDEIEQ VASVVDVLQT PAFLCRQTDF IHACARSGKP VNIKKGQFLA PHDMKNVIDK ARDAAREAGL SEDRFMACER GVSFGYNNLV SDMRSLAIMR ETNAPVVFDA THSVQLPGGQ GTSSGGQREF VPVLARAAVA TGVAGLFMET HPNPAEAKSD GPNAVPLNRM GALLETLVTL DQAVKRNPFL ENDFN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagctgtgc gatttcgagg tcggtctcga ccagcctttc ttcctgatcg cgggtacctg cgtcgtcgaa tcggagcaaa tgacgatcga cacggcgggc cgcctgaagg agatctgcga gaagctgaac gttccgttca tctacaagtc gtcctacgac aaggcaaacc gcagctcggg caagtcgttt cgcggcctcg gaatggacga gggcctgcgg atcctgtccg aggtgaagcg ccagctcggc ctgccggtgc tgaccgacgt gcattcgatc gacgagatcg agcaggtcgc gtcggtcgtc gacgtgctgc agacgccggc gttcctgtgc cgccagaccg acttcatcca tgcgtgcgcg cgctcgggca agccggtcaa catcaagaag ggccagttcc tcgcgccgca cgacatgaag aacgtgatcg acaaggcgcg cgacgccgca cgtgaagccg gtctgtcgga agaccgtttc atggcgtgcg agcgcggcgt ctcgttcggc tacaacaatc tcgtgtcgga catgcgttcg ctcgcgatca tgcgcgagac gaacgcgccg gtcgtgttcg atgcgaccca ctcggtgcaa ttgccgggcg ggcagggcac gagctcgggc ggccaacgcg aattcgtgcc ggtgctcgcc cgtgcggcgg tcgcgacggg cgtcgcgggc ctcttcatgg aaacgcatcc gaatccggcc gaggcgaagt cggacggccc gaatgcggtg ccgctgaacc ggatgggcgc gttgctggag accctcgtca cgctcgacca ggcggtgaag cgcaatccgt tcctggaaaa cgatttcaat taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa