SSGCID: Target Page

BuceA.00100.c

Putative ribose transport system, substrate-binding protein

CENTER ID:	BuceA.00100.c
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00100.c.B1.GE36621	full length		1	316

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00100.c.B1.PW37290	full length		1	316

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.1715
RefSeq:	YP_002230785.1
UniProt:	B4E8T0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNVRFRRRFL TAALAAVAVA AAPAVHAQSA GKPKVALVMK SLANEFFLTM ETGAKEYQKH NANQFDLITN GIKDETDTAN QIRIVEQMIV SKVDAIVIAP ADSKALVPVV KKAVDAGIIV VNIDNRLDPD VLKSKNLNVP FVGPDNRKGA RKIGDYLAKR LKAGDQVGIV EGVSTTTNAQ QRTAGFQDAM KAGGMKVVSV QSGEWEIDKG NAVAAAMLNE YPNLKALLCG NDNMAIGAVS AVRAAGKQGK VLVVGYDNIN AIKPMLKDGR VLATADQYAA KQAVFGIDTA LKAIAEHRKQ ADMSGVVETP VDLVTK

NT Sequence

atgaatgtcc gctttcgccg tcgcttcctg accgccgcgc tcgccgccgt cgcggtggcc gccgcaccgg ccgtccacgc gcaatcggcg ggcaagccga aggtcgcgct cgtgatgaag tcgctcgcca acgagttctt cctgaccatg gaaacgggcg cgaaggaata ccagaaacac aacgcgaacc agttcgacct gatcaccaac ggcatcaagg acgaaaccga cactgcgaac cagatccgca tcgtcgagca gatgatcgtc tcgaaggtcg acgcgatcgt gatcgcaccc gccgattcga aggcgctcgt gccggtcgtg aagaaggccg tcgacgcggg catcatcgtc gtgaacatcg acaaccggct cgaccccgac gtgctgaagt cgaagaacct gaacgtgccg ttcgtcggcc cggacaaccg caagggcgcg cgcaagatcg gcgactacct cgcgaagcgg ctgaaggcgg gcgaccaggt cggcatcgtc gaaggcgtgt cgacgacgac caacgcgcag cagcgcacgg ccggcttcca ggacgcgatg aaggcgggcg ggatgaaggt cgtgtcggtg cagtcgggcg aatgggagat cgacaagggc aatgcggtgg ccgccgcgat gctcaacgaa tacccgaacc tgaaggcgct gctgtgcggt aacgacaaca tggcgatcgg cgccgtgtcg gccgtgcgcg cggccggcaa gcagggcaag gtgctcgtgg tcggctacga caacatcaac gcgatcaagc cgatgctgaa ggacggccgc gtgctcgcga ccgccgacca gtacgcggcg aagcaggccg tgttcggcat cgacacggcg ctcaaggcga tcgccgagca tcgcaagcag gccgacatgt ccggcgtggt cgagacgccg gtggatctcg tgacgaagta a

Details for BuceA.00100.c.B1.GE36621

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	17
PERCENT COVERAGE:	90

Validated AA Sequence

MLGDRLERRV DAEHGLLRRV LVGGREHAAV LQHRLDRVDV VVADHEHLAL LAGRAHGRHG ADRHVVVTAQ QRLQVRVFVE HRGGHRIALV DLPFARLHRH DLHPARLHRV LEAGRALLRV GRRRHAFDDA DLVARLQPLR EVVADLARAL AVVRADERHV QVLRLQHVGV EPVVDVHDDD ARVDGLLHDR HERLRIGGCD HDRVDLRDDH LLDDADLVRS VGFVLDAVGD QVELVRVVFL VFLRARFHGQ EELVGERLHH ERDLRLARRL RVDGRCGGHR DGGERGGQEA TAKA

Validated NT Sequence

tactcactta cttcgtcacg agatccaccg gcgtctcgac cacgccggac atgtcggcct gcttgcgatg ctcggcgatc gccttgagcg ccgtgtcgat gccgaacacg gcctgcttcg ccgcgtactg gtcggcggtc gcgagcacgc ggccgtcctt cagcatcggc ttgatcgcgt tgatgttgtc gtagccgacc acgagcacct tgccctgctt gccggccgcg cgcacggccg acacggcgcc gatcgccatg ttgtcgttac cgcacagcag cgccttcagg ttcgggtatt cgttgagcat cgcggcggcc accgcattgc ccttgtcgat ctcccattcg cccgactgca ccgacacgac cttcatcccg cccgccttca tcgcgtcctg gaagccggcc gtgcgctgct gcgcgttggt cgtcgtcgac acgccttcga cgatgccgac ctggtcgccc gccttcagcc gcttcgcgag gtagtcgccg atcttgcgcg cgcccttgcg gttgtccggg ccgacgaacg gcacgttcag gttcttcgac ttcagcacgt cggggtcgag ccggttgtcg atgttcacga cgatgatgcc cgcgtcgacg gccttcttca cgaccggcac gagcgccttc gaatcggcgg gtgcgatcac gatcgcgtcg accttcgaga cgatcatctg ctcgacgatg cggatctggt tcgcagtgtc ggtttcgtcc ttgatgccgt tggtgatcag gtcgaactgg ttcgcgttgt gtttctggta ttccttcgcg cccgtttcca tggtcaggaa gaactcgttg gcgagcgact tcatcacgag cgcgaccttc ggcttgcccg ccgattgcgc gtggacggcc ggtgcggcgg ccaccgcgac ggcggcgagc gcggcggtca ggaagcgacg gcgaaagcgg

Expected Protein Sequence

MAHHHHHHMN VRFRRRFLTA ALAAVAVAAA PAVHAQSAGK PKVALVMKSL ANEFFLTMET GAKEYQKHNA NQFDLITNGI KDETDTANQI RIVEQMIVSK VDAIVIAPAD SKALVPVVKK AVDAGIIVVN IDNRLDPDVL KSKNLNVPFV GPDNRKGARK IGDYLAKRLK AGDQVGIVEG VSTTTNAQQR TAGFQDAMKA GGMKVVSVQS GEWEIDKGNA VAAAMLNEYP NLKALLCGND NMAIGAVSAV RAAGKQGKVL VVGYDNINAI KPMLKDGRVL ATADQYAAKQ AVFGIDTALK AIAEHRKQAD MSGVVETPVD LVTK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg tccgctttcg ccgtcgcttc ctgaccgccg cgctcgccgc cgtcgcggtg gccgccgcac cggccgtcca cgcgcaatcg gcgggcaagc cgaaggtcgc gctcgtgatg aagtcgctcg ccaacgagtt cttcctgacc atggaaacgg gcgcgaagga ataccagaaa cacaacgcga accagttcga cctgatcacc aacggcatca aggacgaaac cgacactgcg aaccagatcc gcatcgtcga gcagatgatc gtctcgaagg tcgacgcgat cgtgatcgca cccgccgatt cgaaggcgct cgtgccggtc gtgaagaagg ccgtcgacgc gggcatcatc gtcgtgaaca tcgacaaccg gctcgacccc gacgtgctga agtcgaagaa cctgaacgtg ccgttcgtcg gcccggacaa ccgcaagggc gcgcgcaaga tcggcgacta cctcgcgaag cggctgaagg cgggcgacca ggtcggcatc gtcgaaggcg tgtcgacgac gaccaacgcg cagcagcgca cggccggctt ccaggacgcg atgaaggcgg gcgggatgaa ggtcgtgtcg gtgcagtcgg gcgaatggga gatcgacaag ggcaatgcgg tggccgccgc gatgctcaac gaatacccga acctgaaggc gctgctgtgc ggtaacgaca acatggcgat cggcgccgtg tcggccgtgc gcgcggccgg caagcagggc aaggtgctcg tggtcggcta cgacaacatc aacgcgatca agccgatgct gaaggacggc cgcgtgctcg cgaccgccga ccagtacgcg gcgaagcagg ccgtgttcgg catcgacacg gcgctcaagg cgatcgccga gcatcgcaag caggccgaca tgtccggcgt ggtcgagacg ccggtggatc tcgtgacgaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BuceA.00100.c.B1.PW37290

PURIFICATION DATe:	5/20/2013
CONCENTRATION:	23.6mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	110µl
PERCENT IDENTITY:	17
PERCENT COVERAGE:	90

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG TCCGCTTTCG CCGTCGCTTC CTGACCGCCG CGCTCGCCGC CGTCGCGGTG GCCGCCGCAC CGGCCGTCCA CGCGCAATCG GCGGGCAAGC CGAAGGTCGC GCTCGTGATG AAGTCGCTCG CCAACGAGTT CTTCCTGACC ATGGAAACGG GCGCGAAGGA ATACCAGAAA CACAACGCGA ACCAGTTCGA CCTGATCACC AACGGCATCA AGGACGAAAC CGACACTGCG AACCAGATCC GCATCGTCGA GCAGATGATC GTCTCGAAGG TCGACGCGAT CGTGATCGCA CCCGCCGATT CGAAGGCGCT CGTGCCGGTC GTGAAGAAGG CCGTCGACGC GGGCATCATC GTCGTGAACA TCGACAACCG GCTCGACCCC GACGTGCTGA AGTCGAAGAA CCTGAACGTG CCGTTCGTCG GCCCGGACAA CCGCAAGGGC GCGCGCAAGA TCGGCGACTA CCTCGCGAAG CGGCTGAAGG CGGGCGACCA GGTCGGCATC GTCGAAGGCG TGTCGACGAC GACCAACGCG CAGCAGCGCA CGGCCGGCTT CCAGGACGCG ATGAAGGCGG GCGGGATGAA GGTCGTGTCG GTGCAGTCGG GCGAATGGGA GATCGACAAG GGCAATGCGG TGGCCGCCGC GATGCTCAAC GAATACCCGA ACCTGAAGGC GCTGCTGTGC GGTAACGACA ACATGGCGAT CGGCGCCGTG TCGGCCGTGC GCGCGGCCGG CAAGCAGGGC AAGGTGCTCG TGGTCGGCTA CGACAACATC AACGCGATCA AGCCGATGCT GAAGGACGGC CGCGTGCTCG CGACCGCCGA CCAGTACGCG GCGAAGCAGG CCGTGTTCGG CATCGACACG GCGCTCAAGG CGATCGCCGA GCATCGCAAG CAGGCCGACA TGTCCGGCGT GGTCGAGACG CCGGTGGATC TCGTGACGAA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT