SSGCID: Target Page

BuceA.00100.b

Putative solute-binding component of ABC transporter

CENTER ID:	BuceA.00100.b
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00100.b.B2.GE36739	Mature protein( BuceA.00100.b )		33	337

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.6631
RefSeq:	YP_002235398.1
UniProt:	B4ELJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNRTTHTIRR HTLRALLAAL CIAPLGMQGA AHADAPLKIG FLVKMPEQAW FINEQNAASA LGQKENFSVV KIGTPDGEKV LAAIDNLGSQ GAQGFVICAP DVRLGPAIAS RAKRYNMKFV TVDDQLVDSS GKPLPNVPHL GMSATKIGNQ VGQAIADEMK RRGWKPEEVG ALRVTNYELP TAKLRTDGAT QALLANGFRK ENIFDAPQKT TDDEGGFSAA APVLARHPNV KKWVIYALNE ETVLGAVRAT EQLHIPAADV IGVGINGAGE AFAEFQKKEP TGFYGTIAVS STNHGKDSTQ NLVDWIRNGK TPPADTQTSG KLMTRANWQA VRAELGI

NT Sequence

ATGAACCGCA CGACTCACAC GATTCGCCGC CATACCCTGC GCGCGCTGCT GGCCGCGCTC TGCATCGCGC CGCTCGGCAT GCAGGGCGCC GCGCACGCCG ACGCGCCGCT GAAGATCGGC TTCCTCGTGA AGATGCCGGA GCAGGCGTGG TTCATCAACG AGCAGAACGC GGCGTCCGCG CTCGGCCAGA AGGAGAATTT CTCGGTCGTG AAGATCGGCA CGCCGGACGG CGAGAAGGTG CTGGCCGCGA TCGACAACCT CGGTTCGCAG GGCGCGCAGG GCTTCGTGAT CTGCGCGCCG GATGTACGTC TCGGGCCGGC GATCGCGTCG CGCGCGAAGC GCTACAACAT GAAGTTCGTG ACGGTCGACG ACCAGCTCGT CGATTCGAGC GGCAAGCCGC TGCCGAACGT GCCGCACCTC GGGATGTCGG CGACCAAGAT CGGCAACCAG GTCGGCCAGG CGATCGCCGA CGAGATGAAG CGGCGCGGCT GGAAGCCGGA AGAAGTCGGC GCGCTGCGTG TCACCAACTA CGAGCTGCCG ACCGCGAAGC TGCGCACCGA CGGCGCGACG CAGGCGCTGC TCGCGAACGG CTTCCGCAAG GAAAACATCT TCGACGCGCC GCAGAAGACC ACCGACGACG AAGGCGGCTT CAGTGCGGCG GCGCCGGTGC TCGCGCGCCA TCCGAACGTG AAGAAATGGG TGATCTACGC GCTGAACGAG GAAACCGTGC TCGGCGCGGT GCGCGCGACC GAACAGCTGC ACATTCCGGC GGCCGACGTG ATCGGCGTCG GCATCAACGG CGCGGGCGAG GCGTTCGCCG AGTTCCAGAA GAAGGAGCCG ACCGGCTTCT ACGGGACGAT CGCGGTCAGC TCGACGAACC ACGGCAAGGA CAGCACGCAG AACCTCGTCG ACTGGATCCG CAACGGCAAG ACGCCGCCGG CCGACACGCA GACGAGCGGC AAGCTGATGA CGCGCGCGAA CTGGCAGGCC GTGCGCGCCG AACTCGGTAT C

Details for BuceA.00100.b.B2.GE36739

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA DAPLKIGFLV KMPEQAWFIN EQNAASALGQ KENFSVVKIG TPDGEKVLAA IDNLGSQGAQ GFVICAPDVR LGPAIASRAK RYNMKFVTVD DQLVDSSGKP LPNVPHLGMS ATKIGNQVGQ AIADEMKRRG WKPEEVGALR VTNYELPTAK LRTDGATQAL LANGFRKENI FDAPQKTTDD EGGFSAAAPV LARHPNVKKW VIYALNEETV LGAVRATEQL HIPAADVIGV GINGAGEAFA EFQKKEPTGF YGTIAVSSTN HGKDSTQNLV DWIRNGKTPP ADTQTSGKLM TRANWQAVRA ELGI

Validated NT Sequence

tcttactcac ttagataccg agttcggcgc gcacggcctg ccagttcgcg cgcgtcatca gcttgccgct cgtctgcgtg tcggccggcg gcgtcttgcc gttgcggatc cagtcgacga ggttctgcgt gctgtccttg ccgtggttcg tcgagctgac cgcgatcgtc ccgtagaagc cggtcggctc cttcttctgg aactcggcga acgcctcgcc cgcgccgttg atgccgacgc cgatcacgtc ggccgccgga atgtgcagct gttcggtcgc gcgcaccgcg ccgagcacgg tttcctcgtt cagcgcgtag atcacccatt tcttcacgtt cggatggcgc gcgagcaccg gcgccgccgc actgaagccg ccttcgtcgt cggtggtctt ctgcggcgcg tcgaagatgt tttccttgcg gaagccgttc gcgagcagcg cctgcgtcgc gccgtcggtg cgcagcttcg cggtcggcag ctcgtagttg gtgacacgca gcgcgccgac ttcttccggc ttccagccgc gccgcttcat ctcgtcggcg atcgcctggc cgacctggtt gccgatcttg gtcgccgaca tcccgaggtg cggcacgttc ggcagcggct tgccgctcga atcgacgagc tggtcgtcga ccgtcacgaa cttcatgttg tagcgcttcg cgcgcgacgc gatcgccggc ccgagacgta catccggcgc gcagatcacg aagccctgcg cgccctgcga accgaggttg tcgatcgcgg ccagcacctt ctcgccgtcc ggcgtgccga tcttcacgac cgagaaattc tccttctggc cgagcgcgga cgccgcgttc tgctcgttga tgaaccacgc ctgctccggc atcttcacga ggaagccgat cttcagcggc gcgtcggcca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHAD APLKIGFLVK MPEQAWFINE QNAASALGQK ENFSVVKIGT PDGEKVLAAI DNLGSQGAQG FVICAPDVRL GPAIASRAKR YNMKFVTVDD QLVDSSGKPL PNVPHLGMSA TKIGNQVGQA IADEMKRRGW KPEEVGALRV TNYELPTAKL RTDGATQALL ANGFRKENIF DAPQKTTDDE GGFSAAAPVL ARHPNVKKWV IYALNEETVL GAVRATEQLH IPAADVIGVG INGAGEAFAE FQKKEPTGFY GTIAVSSTNH GKDSTQNLVD WIRNGKTPPA DTQTSGKLMT RANWQAVRAE LGI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgacg cgccgctgaa gatcggcttc ctcgtgaaga tgccggagca ggcgtggttc atcaacgagc agaacgcggc gtccgcgctc ggccagaagg agaatttctc ggtcgtgaag atcggcacgc cggacggcga gaaggtgctg gccgcgatcg acaacctcgg ttcgcagggc gcgcagggct tcgtgatctg cgcgccggat gtacgtctcg ggccggcgat cgcgtcgcgc gcgaagcgct acaacatgaa gttcgtgacg gtcgacgacc agctcgtcga ttcgagcggc aagccgctgc cgaacgtgcc gcacctcggg atgtcggcga ccaagatcgg caaccaggtc ggccaggcga tcgccgacga gatgaagcgg cgcggctgga agccggaaga agtcggcgcg ctgcgtgtca ccaactacga gctgccgacc gcgaagctgc gcaccgacgg cgcgacgcag gcgctgctcg cgaacggctt ccgcaaggaa aacatcttcg acgcgccgca gaagaccacc gacgacgaag gcggcttcag tgcggcggcg ccggtgctcg cgcgccatcc gaacgtgaag aaatgggtga tctacgcgct gaacgaggaa accgtgctcg gcgcggtgcg cgcgaccgaa cagctgcaca ttccggcggc cgacgtgatc ggcgtcggca tcaacggcgc gggcgaggcg ttcgccgagt tccagaagaa ggagccgacc ggcttctacg ggacgatcgc ggtcagctcg acgaaccacg gcaaggacag cacgcagaac ctcgtcgact ggatccgcaa cggcaagacg ccgccggccg acacgcagac gagcggcaag ctgatgacgc gcgcgaactg gcaggccgtg cgcgccgaac tcggtatctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat