BuceA.00096.b

Putative acyl-CoA transferase

CENTER ID: BuceA.00096.b
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00096.b.B1.GE36737 Full length( BuceA.00096.b ) 1 442
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3343
RefSeq: YP_002232329.1
UniProt: B4EDD7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALGEHLRQQ LAAKALKRQL ERATDAAAAP GVPGTLAAQT ASARSRFESM PQYQQVRIMR EMGEKLRVDS PFFRVHEGVA GATTQIGGRE YLNFANYNYL GLAGDPAVSS RAKAAIDRYG TSASASRMVA GERPVQRDLE RALAAFYETD DCVAFVSGHA TNVTVIGALF GPGDLVVHDS LAHNSIVQGA QLSGAKRLSF AHNDWQALDE LLSRVRREYR HVLIAIEGLY SMDGDFPDLQ RFVDVKTRHG AFLLVDEAHS LGVLGATGKG IREHCGVAPD QVDMWMGTMS KTLAGCGGFI AGCQPLVDML RHLAPGFLYS VGLAPTLAEA SLAALERLQA EPERVAQLQA RGRQFLTEAR AAGLNTGTSA GFAVVPVITG SSLKAAQWAN AMFDEGINVQ PIFYPAVEEK AARLRFFICS THEPEQISRT VGVLSRLAGR SA
NT Sequence
ATGGCACTGG GAGAGCATCT TCGCCAGCAA CTGGCGGCGA AAGCGTTGAA ACGGCAGCTG GAGCGCGCGA CCGATGCGGC CGCGGCACCG GGCGTGCCCG GCACGCTGGC CGCGCAGACC GCATCGGCGC GCAGCCGCTT CGAATCGATG CCGCAGTATC AGCAGGTCCG GATCATGCGC GAGATGGGCG AGAAGCTGCG CGTCGACTCG CCGTTCTTCC GCGTGCATGA AGGCGTGGCC GGCGCGACGA CGCAGATCGG CGGCCGCGAG TACCTGAACT TCGCGAACTA CAACTACCTC GGCCTGGCCG GCGATCCGGC CGTGTCGTCG CGTGCGAAAG CCGCGATCGA CCGCTACGGC ACGTCGGCCT CCGCGAGCCG GATGGTCGCG GGCGAGCGCC CGGTGCAGCG TGATCTCGAG CGCGCGCTGG CTGCGTTCTA CGAGACCGAC GACTGTGTCG CGTTCGTGAG CGGCCACGCG ACGAACGTGA CCGTGATCGG CGCGCTGTTT GGCCCCGGCG ATCTCGTCGT CCACGATTCG CTCGCGCACA ACAGCATCGT GCAGGGCGCG CAGCTGAGCG GCGCGAAGCG GCTGAGCTTC GCGCACAACG ACTGGCAGGC GCTCGACGAG CTGCTGTCGC GCGTGCGCCG CGAATACCGG CACGTACTGA TCGCGATCGA AGGGCTGTAC AGCATGGACG GTGATTTCCC CGACCTGCAG CGCTTCGTCG ACGTGAAGAC GCGCCACGGC GCATTCCTGC TGGTCGACGA GGCGCATTCG CTCGGCGTGC TCGGCGCGAC CGGCAAGGGC ATTCGCGAGC ATTGCGGCGT GGCGCCCGAC CAGGTCGACA TGTGGATGGG CACGATGAGC AAGACGCTGG CCGGCTGCGG CGGCTTCATC GCCGGCTGCC AGCCGCTGGT CGACATGCTG CGGCACCTGG CGCCGGGCTT CCTGTACAGC GTCGGGCTCG CGCCGACGCT CGCCGAAGCG TCGCTGGCCG CGCTTGAGCG CCTGCAGGCC GAGCCGGAGC GCGTCGCGCA ACTGCAGGCG CGCGGGCGGC AGTTCCTGAC CGAGGCGCGC GCCGCCGGGC TGAACACGGG CACGAGCGCC GGGTTCGCGG TCGTGCCGGT GATCACGGGG AGCTCGCTGA AGGCCGCGCA ATGGGCCAAC GCGATGTTCG ATGAAGGAAT CAACGTGCAG CCGATCTTCT ATCCGGCCGT CGAGGAAAAG GCCGCGCGCC TGCGCTTCTT CATCTGCTCG ACGCATGAGC CCGAGCAGAT CAGCCGGACG GTCGGGGTGT TGTCGCGACT CGCGGGGCGA AGCGCA
Details for BuceA.00096.b.B1.GE36737
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 50kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 23
PERCENT COVERAGE: 93
Validated AA Sequence
MRRADEEAQA RGLFLDGRIE DRLHVDSFIE HRVGPLRGLQ RAPRDHRHDR EPGARARVQP GGARLGQELP PARLQLRDAL RLGLQALKRG QRRFGERRRE PDAVQEARRQ VPQHVDQRLA AGDEAAAAGQ RLAHRAHPHV DLVGRHAAML ANALAGRAEH AERMRLVDQQ ECAVARLHVD EALQVGEITV HAVQPFDRDQ YVPVFAAHAR QQLVERLPVV VREAQPLRAA QLRALHDAVV RERIVDDEIA GAKQRADHGH VRRVAAHERD TVVGLVERSQ RALEITLHRA LARDHPARGG RRAVAVDRGF RTRRHGRIAG QAEVVVVREV QVLAAADLRR RAGHAFMHAE ERRVDAQLLA HLAHDPDLLI LRHRFEAAAR RCGLRGQRAG HARCRGRIGR ALQLPFQRFR RQLLAKMLSQ
Validated NT Sequence
caccccgacc gtccggctga tctgctcggg ctcatgcgtc gagcagatga agaagcgcag gcgcgcggcc ttttcctcga cggccggata gaagatcggc tgcacgttga ttccttcatc gaacatcgcg ttggcccatt gcgcggcctt cagcgagctc cccgtgatca ccggcacgac cgcgaacccg gcgctcgtgc ccgtgttcag cccggcggcg cgcgcctcgg tcaggaactg ccgcccgcgc gcctgcagtt gcgcgacgcg ctccggctcg gcctgcaggc gctcaagcgc ggccagcgac gcttcggcga gcgtcggcgc gagcccgacg ctgtacagga agcccggcgc caggtgccgc agcatgtcga ccagcggctg gcagccggcg atgaagccgc cgcagccggc cagcgtcttg ctcatcgtgc ccatccacat gtcgacctgg tcgggcgcca cgccgcaatg ctcgcgaatg cccttgccgg tcgcgccgag cacgccgagc gaatgcgcct cgtcgaccag caggaatgcg ccgtggcgcg tcttcacgtc gacgaagcgc tgcaggtcgg ggaaatcacc gtccatgctg tacagccctt cgatcgcgat cagtacgtgc cggtattcgc ggcgcacgcg cgacagcagc tcgtcgagcg cctgccagtc gttgtgcgcg aagctcagcc gcttcgcgcc gctcagctgc gcgccctgca cgatgctgtt gtgcgcgagc gaatcgtgga cgacgagatc gccggggcca aacagcgcgc cgatcacggt cacgttcgtc gcgtggccgc tcacgaacgc gacacagtcg tcggtctcgt agaacgcagc cagcgcgcgc tcgagatcac gctgcaccgg gcgctcgccc gcgaccatcc ggctcgcgga ggccgacgtg ccgtagcggt cgatcgcggc tttcgcacgc gacgacacgg ccggatcgcc ggccaggccg aggtagttgt agttcgcgaa gttcaggtac tcgcggccgc cgatctgcgt cgtcgcgccg gccacgcctt catgcacgcg gaagaacggc gagtcgacgc gcagcttctc gcccatctcg cgcatgatcc ggacctgctg atactgcggc atcgattcga agcggctgcg cgccgatgcg gtctgcgcgg ccagcgtgcc gggcacgccc ggtgccgcgg ccgcatcggt cgcgcgctcc agctgccgtt tcaacgcttt cgccgccagt tgctggcgaa gatgctctcc cagt
Expected Protein Sequence
MAHHHHHHMA LGEHLRQQLA AKALKRQLER ATDAAAAPGV PGTLAAQTAS ARSRFESMPQ YQQVRIMREM GEKLRVDSPF FRVHEGVAGA TTQIGGREYL NFANYNYLGL AGDPAVSSRA KAAIDRYGTS ASASRMVAGE RPVQRDLERA LAAFYETDDC VAFVSGHATN VTVIGALFGP GDLVVHDSLA HNSIVQGAQL SGAKRLSFAH NDWQALDELL SRVRREYRHV LIAIEGLYSM DGDFPDLQRF VDVKTRHGAF LLVDEAHSLG VLGATGKGIR EHCGVAPDQV DMWMGTMSKT LAGCGGFIAG CQPLVDMLRH LAPGFLYSVG LAPTLAEASL AALERLQAEP ERVAQLQARG RQFLTEARAA GLNTGTSAGF AVVPVITGSS LKAAQWANAM FDEGINVQPI FYPAVEEKAA RLRFFICSTH EPEQISRTVG VLSRLAGRSA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac tgggagagca tcttcgccag caactggcgg cgaaagcgtt gaaacggcag ctggagcgcg cgaccgatgc ggccgcggca ccgggcgtgc ccggcacgct ggccgcgcag accgcatcgg cgcgcagccg cttcgaatcg atgccgcagt atcagcaggt ccggatcatg cgcgagatgg gcgagaagct gcgcgtcgac tcgccgttct tccgcgtgca tgaaggcgtg gccggcgcga cgacgcagat cggcggccgc gagtacctga acttcgcgaa ctacaactac ctcggcctgg ccggcgatcc ggccgtgtcg tcgcgtgcga aagccgcgat cgaccgctac ggcacgtcgg cctccgcgag ccggatggtc gcgggcgagc gcccggtgca gcgtgatctc gagcgcgcgc tggctgcgtt ctacgagacc gacgactgtg tcgcgttcgt gagcggccac gcgacgaacg tgaccgtgat cggcgcgctg tttggccccg gcgatctcgt cgtccacgat tcgctcgcgc acaacagcat cgtgcagggc gcgcagctga gcggcgcgaa gcggctgagc ttcgcgcaca acgactggca ggcgctcgac gagctgctgt cgcgcgtgcg ccgcgaatac cggcacgtac tgatcgcgat cgaagggctg tacagcatgg acggtgattt ccccgacctg cagcgcttcg tcgacgtgaa gacgcgccac ggcgcattcc tgctggtcga cgaggcgcat tcgctcggcg tgctcggcgc gaccggcaag ggcattcgcg agcattgcgg cgtggcgccc gaccaggtcg acatgtggat gggcacgatg agcaagacgc tggccggctg cggcggcttc atcgccggct gccagccgct ggtcgacatg ctgcggcacc tggcgccggg cttcctgtac agcgtcgggc tcgcgccgac gctcgccgaa gcgtcgctgg ccgcgcttga gcgcctgcag gccgagccgg agcgcgtcgc gcaactgcag gcgcgcgggc ggcagttcct gaccgaggcg cgcgccgccg ggctgaacac gggcacgagc gccgggttcg cggtcgtgcc ggtgatcacg gggagctcgc tgaaggccgc gcaatgggcc aacgcgatgt tcgatgaagg aatcaacgtg cagccgatct tctatccggc cgtcgaggaa aaggccgcgc gcctgcgctt cttcatctgc tcgacgcatg agcccgagca gatcagccgg acggtcgggg tgttgtcgcg actcgcgggg cgaagcgcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat