BuceA.00096.a

8-amino-7-oxononanoate synthase

CENTER ID: BuceA.00096.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00096.a.B1.GE36736 Full length( BuceA.00096.a ) 1 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.697
RefSeq: YP_002229824.1
UniProt: B4E9L6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLLDTLQRG LADLDAQGLR RVRRIADTAC DARMTVNGRE IVGFASNDYL GLAAHPALVA AFAEGAQRYG SGSGGSHLLG GHSRAHARLE DELAGFAGGF SDAPRALYFS TGYMANLAAM TALAGKGATI FSDALNHASL IDGMRLSRAN VQVYPHADTA ALAALLDASE AETKLIVSDT VFSMDGDVAP LAELVALAER HGAWLVVDDA HGFGVLGPQG RGALAAAALR SPHLVYVGTL GKAAGVAGAF VIAHETVIEW LIQRARSYIF TTAAPPAVAH AVSASLKVIA GDEGDARRAH LAALIERTRA LLRNTRWQPV DSHTAVQPLV IGSNDATLAA MRALDAHGLW VPAIRPPTVP AGTSRLRVSL SAAHSFDDFA RLEAALIEAS EAAA
NT Sequence
ATGAGCCTGC TCGACACCCT GCAACGCGGC CTCGCCGACC TCGACGCGCA AGGGCTGCGC CGCGTGCGCC GCATCGCCGA CACCGCGTGC GACGCGCGCA TGACCGTGAA CGGCCGCGAG ATCGTCGGCT TCGCGAGCAA CGACTACCTC GGCCTCGCCG CGCATCCGGC GCTCGTCGCC GCATTCGCGG AAGGCGCGCA GCGCTACGGC TCCGGCAGCG GCGGCTCGCA CCTGCTCGGC GGCCATTCGC GCGCGCACGC CAGACTGGAA GACGAACTCG CGGGCTTCGC GGGCGGCTTC TCCGACGCGC CGCGCGCGCT GTACTTCAGC ACCGGCTACA TGGCGAACCT CGCCGCGATG ACGGCGCTCG CGGGCAAGGG CGCGACGATC TTCTCCGACG CGCTGAACCA CGCGTCGCTG ATCGACGGGA TGCGGCTGTC GCGCGCGAAC GTGCAGGTCT ATCCGCACGC CGATACGGCC GCGCTCGCCG CGCTGCTCGA CGCATCGGAG GCCGAAACGA AGCTGATCGT CAGCGACACC GTGTTCAGCA TGGACGGCGA CGTCGCGCCG CTCGCCGAAC TCGTCGCGCT GGCCGAGCGC CACGGCGCGT GGCTCGTCGT CGACGATGCG CACGGCTTCG GCGTGCTCGG CCCGCAGGGG CGCGGCGCGC TCGCGGCCGC CGCGCTGCGT TCGCCGCATC TGGTGTACGT CGGCACGCTC GGCAAGGCGG CCGGCGTCGC CGGCGCGTTC GTGATCGCAC ACGAAACCGT GATCGAATGG CTGATCCAGC GCGCGCGCAG CTACATCTTC ACGACCGCCG CGCCGCCGGC CGTCGCGCAT GCGGTCTCCG CGAGCCTGAA GGTGATCGCG GGCGACGAAG GCGACGCGCG CCGCGCGCAC CTCGCCGCGC TGATCGAACG CACGCGTGCG CTGCTGCGCA ACACGCGCTG GCAGCCGGTC GATTCGCACA CGGCCGTGCA GCCGCTCGTG ATCGGCAGCA ACGATGCGAC GCTCGCGGCG ATGCGCGCGC TCGACGCACA CGGCCTGTGG GTGCCCGCGA TCCGGCCGCC GACGGTGCCG GCCGGCACGT CGCGGCTGCG CGTGTCGCTG TCGGCCGCGC ATTCGTTCGA CGATTTCGCC AGGCTCGAAG CCGCGTTGAT CGAAGCTAGC GAGGCAGCCG CA
Details for BuceA.00096.a.B1.GE36736
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 24
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMS LLDTLQRGLA DLDAQGLRRV RRIADTAXXX RMTVNGREIV GFASNDYLGL AAXXXLXXHX WKARXGXXPX AAXXXCXXGX XAXXPHWKXT RXLXGRXXXX XXRAVXXXXX XXXXAMMXXA GXVXXIXXXX XXXXXXIEXX XLLXXXXXXX XXXXXDAXXX LXVXXIXPXX MTXXXXVXXX XXKGPXXYXX XXEXXXXXXX XXAXXXXXXX XXXXXXXXXX XXXXXXXXXA XXXXXXXXXX XXXXPXXXKX XXXXXPPXXX XXXXXXXXXX XXPXPXXXXX XXTXXXXXXX XXXXXXXXXX XXLXXXX
Validated NT Sequence
cnnnnnnnna ttttnnnnnn tttaagaagg aganatacca tggctcacca ccaccaccac catatgagcc tgctcgacac cctgcaacgc ggcctcgccg acctcgacgc gcaagggctg cgccgcgtgc gccgcatcgc cgacaccgcg tgnnannngc gcatgaccgt gaacggccgc gagatcgtcg gcttcgcgag caacgactac ctcggcctcg ccgcgcntnn ggnnctcgnn ncgcattgnt ggaaggcgcg cnnnggtann gntccgnnng cggcggntcn nngctgctnn ncgggnnttn nngcnnnnan gccacactgg aaganaactc gcgnnctnnn cgggcggntt ntnnganncc ncgnncgcgc tgtnnncncn gnnnnnnngn cnnnnnnnnn ngcgatgatg gnnnncgcgg gnanggtnnn nnanatcntc nggnacnagn nnannnanan nnanntnatc gaggnnnngn ngttgttgag nnngannnna nnngnctnnn nncanancna naangacgcn ntcnccnngc tgnnngtnnc nnngatcgng ccnnngtnga tgacnnnnng nnngncngtn ntgtnnntga nnnnnaaggg accnngatnn tatnannatc ncnaagagnt nnnannnnnn nngannnnnn nnnncgcnng tnnntgnnnn nnannnnann cnnnnnnnnn nngtnnnnnn nnnnnnnntt ncnnnnnann nnntcncnnc ntanncgcnn tnnnnnnnnn nancncnnna nnnnnnnnnn nnntannnnc cccnncnnct nnnaaanntn ncnnnannnn cnncccnccg ttnnnnnnnn nnnttnncnn nnnnnnnnnn nnntnnncnn tnnnnccttn tccnnnntnn tntnnnnnnn cnttnacncn nnnnntntnt nnnnncnnnt nnnnnnnnnt nnnntnnnnn tnnnntnnnn cnnanctnnc ccnnnnnnnt c
Expected Protein Sequence
MAHHHHHHMS LLDTLQRGLA DLDAQGLRRV RRIADTACDA RMTVNGREIV GFASNDYLGL AAHPALVAAF AEGAQRYGSG SGGSHLLGGH SRAHARLEDE LAGFAGGFSD APRALYFSTG YMANLAAMTA LAGKGATIFS DALNHASLID GMRLSRANVQ VYPHADTAAL AALLDASEAE TKLIVSDTVF SMDGDVAPLA ELVALAERHG AWLVVDDAHG FGVLGPQGRG ALAAAALRSP HLVYVGTLGK AAGVAGAFVI AHETVIEWLI QRARSYIFTT AAPPAVAHAV SASLKVIAGD EGDARRAHLA ALIERTRALL RNTRWQPVDS HTAVQPLVIG SNDATLAAMR ALDAHGLWVP AIRPPTVPAG TSRLRVSLSA AHSFDDFARL EAALIEASEA AA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc tgctcgacac cctgcaacgc ggcctcgccg acctcgacgc gcaagggctg cgccgcgtgc gccgcatcgc cgacaccgcg tgcgacgcgc gcatgaccgt gaacggccgc gagatcgtcg gcttcgcgag caacgactac ctcggcctcg ccgcgcatcc ggcgctcgtc gccgcattcg cggaaggcgc gcagcgctac ggctccggca gcggcggctc gcacctgctc ggcggccatt cgcgcgcgca cgccagactg gaagacgaac tcgcgggctt cgcgggcggc ttctccgacg cgccgcgcgc gctgtacttc agcaccggct acatggcgaa cctcgccgcg atgacggcgc tcgcgggcaa gggcgcgacg atcttctccg acgcgctgaa ccacgcgtcg ctgatcgacg ggatgcggct gtcgcgcgcg aacgtgcagg tctatccgca cgccgatacg gccgcgctcg ccgcgctgct cgacgcatcg gaggccgaaa cgaagctgat cgtcagcgac accgtgttca gcatggacgg cgacgtcgcg ccgctcgccg aactcgtcgc gctggccgag cgccacggcg cgtggctcgt cgtcgacgat gcgcacggct tcggcgtgct cggcccgcag gggcgcggcg cgctcgcggc cgccgcgctg cgttcgccgc atctggtgta cgtcggcacg ctcggcaagg cggccggcgt cgccggcgcg ttcgtgatcg cacacgaaac cgtgatcgaa tggctgatcc agcgcgcgcg cagctacatc ttcacgaccg ccgcgccgcc ggccgtcgcg catgcggtct ccgcgagcct gaaggtgatc gcgggcgacg aaggcgacgc gcgccgcgcg cacctcgccg cgctgatcga acgcacgcgt gcgctgctgc gcaacacgcg ctggcagccg gtcgattcgc acacggccgt gcagccgctc gtgatcggca gcaacgatgc gacgctcgcg gcgatgcgcg cgctcgacgc acacggcctg tgggtgcccg cgatccggcc gccgacggtg ccggccggca cgtcgcggct gcgcgtgtcg ctgtcggccg cgcattcgtt cgacgatttc gccaggctcg aagccgcgtt gatcgaagct agcgaggcag ccgcatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat