BuceA.00095.g

Putative arylesterase

CENTER ID: BuceA.00095.g
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00095.g.B2.GE36735 Mature protein( BuceA.00095.g ) 17 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.7044
RefSeq: YP_002153729.1
UniProt: B4EQA5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRWLIAVIWW AMPFAWAYDA AAAPLSYTVT STDGVKLAVQ ESGDPDGVPV ILIHGLLGSR LNWDAQVQSA ELRGFRIITY DLRGHGLSDK PSGAQPYRDG TRWGDDLAAV IDGTHARKPV VVGWSLGGVV ISNYLAQYGD RAIAGAVYVD GVVELAPGQI VDHPDVYRDM NAADLKTHLD GERTFVGLCF NRRPDAATFE RLLANAAMAS WDMQKAIPTM SVFAAAGLGH ARVPLLFIYG GRDALVDTQA TLARAAALNS RIVSKVYAAS GHAPFIEEAG RFNRDLAAFV RSASAQ
NT Sequence
ATGCGCTGGC TGATTGCCGT GATCTGGTGG GCGATGCCGT TCGCGTGGGC GTACGACGCG GCCGCCGCGC CGCTGAGCTA TACCGTGACG TCGACGGACG GCGTGAAGCT GGCCGTTCAG GAAAGCGGCG ACCCGGACGG CGTTCCGGTC ATCCTGATTC ACGGCCTGCT CGGCAGCCGC CTGAACTGGG ACGCGCAGGT CCAGAGCGCC GAACTGCGCG GCTTCCGGAT CATCACGTAC GACCTGCGCG GCCACGGGCT GTCGGACAAG CCGTCCGGCG CGCAGCCGTA TCGCGACGGA ACCCGCTGGG GCGACGATCT CGCGGCGGTC ATCGACGGCA CGCATGCGCG CAAACCGGTC GTGGTCGGCT GGTCGCTCGG CGGCGTGGTG ATCTCGAACT ACCTCGCCCA ATACGGCGAC CGCGCGATCG CGGGCGCGGT GTACGTCGAC GGCGTGGTCG AGCTTGCGCC GGGCCAGATC GTCGATCATC CGGACGTGTA CCGCGACATG AATGCGGCGG ATCTGAAAAC GCATCTCGAC GGCGAGCGGA CTTTCGTCGG TCTGTGCTTC AACCGCCGGC CGGATGCCGC GACGTTCGAG CGCTTGCTCG CCAACGCCGC GATGGCGTCG TGGGACATGC AAAAGGCCAT CCCGACGATG TCGGTATTCG CCGCCGCGGG GCTCGGCCAC GCGCGCGTGC CGCTGCTGTT CATCTACGGC GGGCGCGACG CGCTGGTCGA CACGCAGGCG ACGCTGGCTC GCGCGGCCGC ACTGAACTCA CGCATCGTCA GCAAGGTGTA CGCGGCATCC GGTCACGCGC CGTTCATCGA GGAAGCCGGG CGGTTCAATC GCGATCTGGC CGCGTTCGTC CGGTCGGCGT CTGCGCAA
Details for BuceA.00095.g.B2.GE36735
HARVESTED ON: data unavailable
SEQUENCED ON: 2/2/2013
EXPECTED MW: 32kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Data Unavailable
SOLUBLE EXPRESSION LEVEL Data Unavailable
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA YDAAAAPLSY TVTSTDGVKL AVQESGDPDG VPVILIHGLL GSRLNWDAQV QSAELRGFRI ITYDLRGHGL SDKPSGAQPY RDGTRWGDDL AAVIDGTHAR KPVVVGWSLG GVVISNYLAQ YGDRAIAGAV YVDGVVELAP GQIVDHPDVY RDMNAADLKT HLDGERTFVG LCFNRRPDAA TFERLLANAA MASWDMQKAI PTMSVFAAAG LGHARVPLLF IYGGRDALVD TXATLARAAA LNSRIVSKVY AASGHAPFIE EAGRFNRDLA AFVRSASAQ
Validated NT Sequence
cnntananat tttnnnnnnn ttaagaagga gnnataccat ggctcaccac caccaccacc atatggcgta cgacgcggcc gccgcgccgc tgagctatac cgtgacgtcg acggacggcg tgaagctggc cgttcaggaa agcggcgacc cggacggcgt tccggtcatc ctgattcacg gcctgctcgg cagccgcctg aactgggacg cgcaggtcca gagcgccgaa ctgcgcggct tccggatcat cacgtacgac ctgcgcggcc acgggctgtc ggacaagccg tccggcgcgc agccgtatcg cgacggaacc cgctggggcg acgatctcgc ggcggtcatc gacggcacgc atgcgcgcaa accggtcgtg gtcggctggt cgctcggcgg cgtggtgatc tcgaactacc tcgcccaata cggcgaccgc gcgatcgcgg gcgcggtgta cgtcgacggc gtggtcgagc ttgcgccggg ccagatcgtc gatcatccgg acgtgtaccg cgacatgaat gcggcggatc tgaaaacgca tctcgacggc gagcggactt tcgtcggtct gtgcttcaac cgccggccgg atgccgcgac gttcgagcgc ttgctcgcca acgccgcgat ggcgtcgtgg gacatgcaaa aggccatccc gacgatgtcg gtattcgccg ccgcggggct cggccacgcg cgcgtgccgc tgctgttcat ctacggcggg cgcgacgcgc tggtcgacac gcangcgacg ctggctcgcg cggccgcact gaactcacgc atcgtcagca aggtgtacgc ggcatccggt cacgcgccgt tcatcgagga agccgggcgg ttcaatcgcg atctggccgc gttcgtccgg tcggcgtctg cncaataagt gagtanatag gannnnntgn nnnnnnnccn annnnnnann nnnnnnnnnn nnaangnnnn nnnnnnnnnn nnncnnnnnc cnnnnnnnnn cnnnnnngnn nnnnt
Expected Protein Sequence
MAHHHHHHAY DAAAAPLSYT VTSTDGVKLA VQESGDPDGV PVILIHGLLG SRLNWDAQVQ SAELRGFRII TYDLRGHGLS DKPSGAQPYR DGTRWGDDLA AVIDGTHARK PVVVGWSLGG VVISNYLAQY GDRAIAGAVY VDGVVELAPG QIVDHPDVYR DMNAADLKTH LDGERTFVGL CFNRRPDAAT FERLLANAAM ASWDMQKAIP TMSVFAAAGL GHARVPLLFI YGGRDALVDT QATLARAAAL NSRIVSKVYA ASGHAPFIEE AGRFNRDLAA FVRSASAQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgtacg acgcggccgc cgcgccgctg agctataccg tgacgtcgac ggacggcgtg aagctggccg ttcaggaaag cggcgacccg gacggcgttc cggtcatcct gattcacggc ctgctcggca gccgcctgaa ctgggacgcg caggtccaga gcgccgaact gcgcggcttc cggatcatca cgtacgacct gcgcggccac gggctgtcgg acaagccgtc cggcgcgcag ccgtatcgcg acggaacccg ctggggcgac gatctcgcgg cggtcatcga cggcacgcat gcgcgcaaac cggtcgtggt cggctggtcg ctcggcggcg tggtgatctc gaactacctc gcccaatacg gcgaccgcgc gatcgcgggc gcggtgtacg tcgacggcgt ggtcgagctt gcgccgggcc agatcgtcga tcatccggac gtgtaccgcg acatgaatgc ggcggatctg aaaacgcatc tcgacggcga gcggactttc gtcggtctgt gcttcaaccg ccggccggat gccgcgacgt tcgagcgctt gctcgccaac gccgcgatgg cgtcgtggga catgcaaaag gccatcccga cgatgtcggt attcgccgcc gcggggctcg gccacgcgcg cgtgccgctg ctgttcatct acggcgggcg cgacgcgctg gtcgacacgc aggcgacgct ggctcgcgcg gccgcactga actcacgcat cgtcagcaag gtgtacgcgg catccggtca cgcgccgttc atcgaggaag ccgggcggtt caatcgcgat ctggccgcgt tcgtccggtc ggcgtctgcg caatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat