SSGCID: Target Page

BuceA.00095.f

Putative hydrolase

CENTER ID:	BuceA.00095.f
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00095.f.B1.GE36734	Full length( BuceA.00095.f )		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.6841
RefSeq:	YP_002153544.1
UniProt:	B4EPR8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHAEPDDELV RFDAHGGTPL PAADAEGWID HDGARMWHAS FGQGPAVVLL HGGLGHGGNW GNQVPALLAA GYRAIVIDSR GHGRSTRDDR PYSYERMASD VLAVLDALHV DRARFVGWSD GACIALVLAA RAPERAAGVF FFACNMDPGG TKEMVPSPLI DHCFARHRKD YARLSATPDQ FDAFVAAVSE MMRTQPDYTA QDLAAIAVPV AIVQGEHDEF IRPEHAAYLA RTIPGATLTI LPGVSHFAPL QRPERFNAAM LAFLERLPG

NT Sequence

ATGCATGCGG AACCCGACGA CGAACTCGTT CGCTTCGACG CGCACGGCGG CACGCCGCTG CCGGCGGCCG ACGCGGAAGG ATGGATCGAC CACGACGGCG CGCGCATGTG GCACGCGTCG TTCGGCCAGG GGCCGGCGGT CGTGCTGCTG CACGGCGGCC TCGGCCACGG CGGCAACTGG GGCAACCAGG TGCCGGCGCT GCTCGCGGCC GGCTACCGCG CGATCGTGAT CGACAGCCGC GGGCACGGTC GCAGCACGCG CGACGACCGA CCCTATTCGT ACGAACGCAT GGCATCGGAC GTGCTGGCCG TGCTCGACGC GCTGCACGTC GACCGTGCAC GCTTCGTCGG CTGGAGCGAC GGCGCGTGCA TCGCGCTCGT GCTGGCCGCC CGCGCACCGG AACGCGCGGC CGGCGTGTTC TTCTTCGCGT GCAACATGGA TCCGGGCGGC ACGAAGGAGA TGGTGCCGAG CCCGCTGATC GACCACTGTT TCGCGCGGCA TCGCAAGGAC TATGCGCGAC TGTCCGCGAC ACCGGACCAG TTCGATGCGT TCGTCGCGGC CGTCAGCGAA ATGATGCGCA CGCAACCCGA TTACACCGCG CAGGACCTCG CGGCGATCGC CGTGCCGGTG GCGATCGTGC AGGGCGAGCA CGACGAATTC ATCCGGCCCG AGCATGCGGC GTACTTGGCA CGCACGATCC CCGGCGCGAC GCTGACGATC CTGCCCGGCG TGAGCCATTT CGCGCCGCTG CAGCGGCCCG AACGGTTCAA CGCGGCGATG CTGGCGTTTC TCGAGCGGCT GCCGGGA

Details for BuceA.00095.f.B1.GE36734

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMH AEPDDELVRF DAHGGTPLPA ADAEGWIDHD GARMWHASFG QGPAVVLLHG GLGHGGNWGN QVPALLAAGY RAIVIDSRGH GRSTRDDRPY SYERMASDVL AVLDALHVDR ARFVGWSDGA CIALVLAARA PERAAGVFFF ACNMDPGGTK EMVPSPLIDH CFARHRKDYA RLSATPDQFD AFVAAVSEMM RTQPDYTAQD LAAIAVPVAI VQGEHDEFIR PEHAAYLART IPGATLTILP GVSHFAPLQR PERFNAAMLA FLERLPG

Validated NT Sequence

tcttactcac ttatcccggc agccgctcga gaaacgccag catcgccgcg ttgaaccgtt cgggccgctg cagcggcgcg aaatggctca cgccgggcag gatcgtcagc gtcgcgccgg ggatcgtgcg tgccaagtac gccgcatgct cgggccggat gaattcgtcg tgctcgccct gcacgatcgc caccggcacg gcgatcgccg cgaggtcctg cgcggtgtaa tcgggttgcg tgcgcatcat ttcgctgacg gccgcgacga acgcatcgaa ctggtccggt gtcgcggaca gtcgcgcata gtccttgcga tgccgcgcga aacagtggtc gatcagcggg ctcggcacca tctccttcgt gccgcccgga tccatgttgc acgcgaagaa gaacacgccg gccgcgcgtt ccggtgcgcg ggcggccagc acgagcgcga tgcacgcgcc gtcgctccag ccgacgaagc gtgcacggtc gacgtgcagc gcgtcgagca cggccagcac gtccgatgcc atgcgttcgt acgaataggg tcggtcgtcg cgcgtgctgc gaccgtgccc gcggctgtcg atcacgatcg cgcggtagcc ggccgcgagc agcgccggca cctggttgcc ccagttgccg ccgtggccga ggccgccgtg cagcagcacg accgccggcc cctggccgaa cgacgcgtgc cacatgcgcg cgccgtcgtg gtcgatccat ccttccgcgt cggccgccgg cagcggcgtg ccgccgtgcg cgtcgaagcg aacgagttcg tcgtcgggtt ccgcatgcat atggtggtgg tggtggtgag ccatg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatg cggaacccga cgacgaactc gttcgcttcg acgcgcacgg cggcacgccg ctgccggcgg ccgacgcgga aggatggatc gaccacgacg gcgcgcgcat gtggcacgcg tcgttcggcc aggggccggc ggtcgtgctg ctgcacggcg gcctcggcca cggcggcaac tggggcaacc aggtgccggc gctgctcgcg gccggctacc gcgcgatcgt gatcgacagc cgcgggcacg gtcgcagcac gcgcgacgac cgaccctatt cgtacgaacg catggcatcg gacgtgctgg ccgtgctcga cgcgctgcac gtcgaccgtg cacgcttcgt cggctggagc gacggcgcgt gcatcgcgct cgtgctggcc gcccgcgcac cggaacgcgc ggccggcgtg ttcttcttcg cgtgcaacat ggatccgggc ggcacgaagg agatggtgcc gagcccgctg atcgaccact gtttcgcgcg gcatcgcaag gactatgcgc gactgtccgc gacaccggac cagttcgatg cgttcgtcgc ggccgtcagc gaaatgatgc gcacgcaacc cgattacacc gcgcaggacc tcgcggcgat cgccgtgccg gtggcgatcg tgcagggcga gcacgacgaa ttcatccggc ccgagcatgc ggcgtacttg gcacgcacga tccccggcgc gacgctgacg atcctgcccg gcgtgagcca tttcgcgccg ctgcagcggc ccgaacggtt caacgcggcg atgctggcgt ttctcgagcg gctgccggga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat