BuceA.00095.e

Non-heme chloroperoxidase

CENTER ID: BuceA.00095.e
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00095.e.B1.GE36733 Full length( BuceA.00095.e ) 1 270
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.5880
RefSeq: YP_002234710.1
UniProt: B4EFH6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKDGVEIFY KDWGKGQPIV FSHGWPLSAD DWDAQMLFFV EHGYRVIAHD RRGHGRSTQT ADGNDMNHYA ADLADLTEAL DIRNAVHIGH STGGGEVAAY VARHGMKRTS KIVLVSAVPP VMVKSERNPG GTPIEVFDGF RKQLAANRAQ FYLDVPSGPF YGFNRAGAKV SEGVIRNWWR QGMMGGIKAQ YDCIKAFSET DFTEDLKKIT QPALVLHGDD DQVVPYKDAG VLSAKLLKNA RLRIYPGYPH GMLTTNADVI NADILKFIKS
NT Sequence
GTGACGAAGG ACGGCGTCGA GATCTTCTAC AAGGATTGGG GCAAAGGCCA GCCGATCGTC TTCAGTCACG GTTGGCCGCT GTCGGCGGAT GACTGGGACG CTCAAATGCT GTTCTTCGTC GAGCATGGTT ATCGCGTCAT CGCCCATGAT CGTCGTGGGC ATGGGCGATC CACGCAAACA GCCGACGGCA ATGACATGAA TCACTACGCT GCCGATCTCG CGGACCTGAC CGAGGCGCTC GATATCAGGA ATGCCGTCCA CATCGGGCAC TCGACCGGGG GCGGGGAAGT TGCTGCCTAC GTAGCGCGTC ATGGCATGAA GCGGACTTCA AAGATCGTTC TGGTCAGCGC AGTGCCACCG GTCATGGTGA AGAGCGAGCG CAACCCCGGC GGAACGCCGA TTGAAGTCTT CGACGGCTTC AGGAAGCAGC TTGCCGCCAA CCGGGCGCAG TTCTACCTCG ACGTGCCGTC GGGACCGTTT TACGGCTTCA ACCGGGCGGG CGCGAAGGTG TCCGAAGGCG TCATCCGCAA TTGGTGGCGT CAGGGCATGA TGGGTGGAAT CAAGGCTCAA TACGACTGCA TCAAGGCCTT CTCGGAAACG GATTTCACCG AAGACTTGAA GAAGATCACG CAACCGGCGC TGGTCCTGCA TGGTGACGAC GACCAGGTCG TGCCATACAA GGATGCCGGC GTGCTGTCCG CAAAGCTGCT CAAGAACGCG AGGTTGAGAA TCTATCCGGG CTACCCGCAC GGGATGCTGA CGACCAATGC GGATGTCATC AACGCGGACA TTCTGAAGTT CATCAAGAGC
Details for BuceA.00095.e.B1.GE36733
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TKDGVEIFYK DWGKGQPIVF SHGWPLSADD WDAQMLFFVE HGYRVIAHDR RGHGRSTQTA DGNDMNHYAA DLADLTEALD IRNAVHIGHS TGGGEVAAYV ARHGMKRTSK IVLVSAVPPV MVKSERNPGG TPIEVFDGFR KQLAANRAQF YLDVPSGPFY GFNRAGAKVS EGVIRNWWRQ GMMGGIKAQY DCIKAFSETD FTEDLKKITQ PALVLHGDDD QVVPYKDAGV LSAKLLKNAR LRIYPGYPHG MLTTNADVIN ADILKFIKS
Validated NT Sequence
atcttactca cttagctctt gatgaacttc agaatgtccg cgttgatgac atccgcattg gtcgtcagca tcccgtgcgg gtagcccgga tagattctca acctcgcgtt cttgagcagc tttgcggaca gcacgccggc atccttgtat ggcacgacct ggtcgtcgtc accatgcagg accagcgccg gttgcgtgat cttcttcaag tcttcggtga aatccgtttc cgagaaggcc ttgatgcagt cgtattgagc cttgattcca cccatcatgc cctgacgcca ccaattgcgg atgacgcctt cggacacctt cgcgcccgcc cggttgaagc cgtaaaacgg tcccgacggc acgtcgaggt agaactgcgc ccggttggcg gcaagctgct tcctgaagcc gtcgaagact tcaatcggcg ttccgccggg gttgcgctcg ctcttcacca tgaccggtgg cactgcgctg accagaacga tctttgaagt ccgcttcatg ccatgacgcg ctacgtaggc agcaacttcc ccgcccccgg tcgagtgccc gatgtggacg gcattcctga tatcgagcgc ctcggtcagg tccgcgagat cggcagcgta gtgattcatg tcattgccgt cggctgtttg cgtggatcgc ccatgcccac gacgatcatg ggcgatgacg cgataaccat gctcgacgaa gaacagcatt tgagcgtccc agtcatccgc cgacagcggc caaccgtgac tgaagacgat cggctggcct ttgccccaat ccttgtagaa gatctcgacg ccgtccttcg tcaccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMT KDGVEIFYKD WGKGQPIVFS HGWPLSADDW DAQMLFFVEH GYRVIAHDRR GHGRSTQTAD GNDMNHYAAD LADLTEALDI RNAVHIGHST GGGEVAAYVA RHGMKRTSKI VLVSAVPPVM VKSERNPGGT PIEVFDGFRK QLAANRAQFY LDVPSGPFYG FNRAGAKVSE GVIRNWWRQG MMGGIKAQYD CIKAFSETDF TEDLKKITQP ALVLHGDDDQ VVPYKDAGVL SAKLLKNARL RIYPGYPHGM LTTNADVINA DILKFIKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacga aggacggcgt cgagatcttc tacaaggatt ggggcaaagg ccagccgatc gtcttcagtc acggttggcc gctgtcggcg gatgactggg acgctcaaat gctgttcttc gtcgagcatg gttatcgcgt catcgcccat gatcgtcgtg ggcatgggcg atccacgcaa acagccgacg gcaatgacat gaatcactac gctgccgatc tcgcggacct gaccgaggcg ctcgatatca ggaatgccgt ccacatcggg cactcgaccg ggggcgggga agttgctgcc tacgtagcgc gtcatggcat gaagcggact tcaaagatcg ttctggtcag cgcagtgcca ccggtcatgg tgaagagcga gcgcaacccc ggcggaacgc cgattgaagt cttcgacggc ttcaggaagc agcttgccgc caaccgggcg cagttctacc tcgacgtgcc gtcgggaccg ttttacggct tcaaccgggc gggcgcgaag gtgtccgaag gcgtcatccg caattggtgg cgtcagggca tgatgggtgg aatcaaggct caatacgact gcatcaaggc cttctcggaa acggatttca ccgaagactt gaagaagatc acgcaaccgg cgctggtcct gcatggtgac gacgaccagg tcgtgccata caaggatgcc ggcgtgctgt ccgcaaagct gctcaagaac gcgaggttga gaatctatcc gggctacccg cacgggatgc tgacgaccaa tgcggatgtc atcaacgcgg acattctgaa gttcatcaag agctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat