SSGCID: Target Page

BuceA.00095.d

Non-heme chloroperoxidase

CENTER ID:	BuceA.00095.d
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00095.d.B1.GE36732	Full length( BuceA.00095.d )		1	276

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.5877
RefSeq:	YP_002234707.1
UniProt:	B4EFH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGYVTTKDGV QIFYKDWGPR DAQVIFFHHG WPLSADDWDA QMLFFLSHGY RVIAHDRRGH GRSSQVWDGH DMDHYADDVA AVVNHLGVQG AVHVGHSTGG GEVIHYVARH GEDRVSKAVL ISAVPPLMVK TDKNPGGLPK DVFDNLQAQL AANRAQFYYD VPAGPFYGYN RAGAKPSQGV IWNWWRQGMM GSAKAHYDGI VAFSQTDFTE DLKSTTIPVL VMHGDDDQIV PYADSGLLSA KLARNSTLKT YKGFPHGMPT SNADTINADL LAFLRG

NT Sequence

ATGGGATACG TCACAACAAA GGATGGCGTT CAGATCTTCT ACAAGGACTG GGGTCCGCGC GACGCGCAGG TCATCTTCTT CCATCACGGT TGGCCGCTCA GCGCGGACGA CTGGGATGCC CAGATGCTCT TTTTCCTGTC GCACGGGTAC CGGGTCATCG CGCATGACCG CCGCGGTCAC GGACGCTCCA GCCAGGTGTG GGACGGTCAT GACATGGATC ATTACGCGGA CGACGTCGCC GCTGTCGTGA ACCATCTCGG CGTGCAAGGC GCGGTTCATG TCGGCCACTC CACCGGCGGT GGCGAAGTCA TTCACTACGT CGCCCGTCAT GGCGAAGACC GCGTCTCGAA GGCCGTGCTG ATCAGCGCTG TGCCGCCGCT GATGGTCAAG ACAGACAAGA ACCCGGGCGG CCTGCCGAAG GACGTGTTCG ATAACCTGCA GGCGCAACTG GCCGCGAACC GTGCTCAGTT TTATTACGAC GTTCCGGCCG GCCCGTTCTA CGGCTACAAC CGTGCCGGCG CGAAACCGTC GCAAGGCGTG ATCTGGAACT GGTGGCGCCA AGGCATGATG GGCAGCGCCA AGGCGCATTA CGACGGCATC GTCGCGTTCT CGCAGACCGA CTTTACCGAG GACCTGAAGA GCACCACGAT TCCCGTGCTC GTGATGCATG GTGATGACGA TCAGATCGTC CCCTATGCCG ATTCCGGCTT GCTGTCGGCG AAGCTCGCTC GGAACAGCAC GCTGAAGACC TACAAGGGCT TCCCGCACGG CATGCCTACG TCCAACGCCG ACACGATCAA CGCCGATCTG CTCGCGTTCC TGCGAGGG

Details for BuceA.00095.d.B1.GE36732

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	79

Validated AA Sequence

MLFFLSHGYR VIAHDRRGHG RSSQVWDGHD MDHYADDVAA VVNHLGVQGA VHVGHSTGGG EVIHYVARHG EDRVSKAVLI SAVPPLMVKT DKNPGGLPKD VFDNLQAQLA ANRAQFYYDV PAGPFYGYNR AGAKPSQGVI WNWWRQGMMG SAKAHYDGIV AFSQTDFTED LKSTTIPVLV MHGDDDQIVP YADSGLLSAK LARNSTLKTY KGFPHGMPTS NADTI

Validated NT Sequence

gttgatcgtg tcggcgttgg acgtaggcat gccgtgcggg aagcccttgt aggtcttcag cgtgctgttc cgagcgagct tcgccgacag caagccggaa tcggcatagg ggacgatctg atcgtcatca ccatgcatca cgagcacggg aatcgtggtg ctcttcaggt cctcggtaaa gtcggtctgc gagaacgcga cgatgccgtc gtaatgcgcc ttggcgctgc ccatcatgcc ttggcgccac cagttccaga tcacgccttg cgacggtttc gcgccggcac ggttgtagcc gtagaacggg ccggccggaa cgtcgtaata aaactgagca cggttcgcgg ccagttgcgc ctgcaggtta tcgaacacgt ccttcggcag gccgcccggg ttcttgtctg tcttgaccat cagcggcggc acagcgctga tcagcacggc cttcgagacg cggtcttcgc catgacgggc gacgtagtga atgacttcgc caccgccggt ggagtggccg acatgaaccg cgccttgcac gccgagatgg ttcacgacag cggcgacgtc gtccgcgtaa tgatccatgt catgaccgtc ccacacctgg ctggagcgtc cgtgaccgcg gcggtcatgc gcgatgaccc ggtacccgtg cgacaggaaa aagagcatct gggcatccca gtcgtccgcg ctgagcggcc aaccgtgatg gaagaagatg acctgcgcgt cgcgcggacc ccagtccttg tagaagatct gaacgccatc ctttgttgtg acgt

Expected Protein Sequence

MAHHHHHHMG YVTTKDGVQI FYKDWGPRDA QVIFFHHGWP LSADDWDAQM LFFLSHGYRV IAHDRRGHGR SSQVWDGHDM DHYADDVAAV VNHLGVQGAV HVGHSTGGGE VIHYVARHGE DRVSKAVLIS AVPPLMVKTD KNPGGLPKDV FDNLQAQLAA NRAQFYYDVP AGPFYGYNRA GAKPSQGVIW NWWRQGMMGS AKAHYDGIVA FSQTDFTEDL KSTTIPVLVM HGDDDQIVPY ADSGLLSAKL ARNSTLKTYK GFPHGMPTSN ADTINADLLA FLRG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggat acgtcacaac aaaggatggc gttcagatct tctacaagga ctggggtccg cgcgacgcgc aggtcatctt cttccatcac ggttggccgc tcagcgcgga cgactgggat gcccagatgc tctttttcct gtcgcacggg taccgggtca tcgcgcatga ccgccgcggt cacggacgct ccagccaggt gtgggacggt catgacatgg atcattacgc ggacgacgtc gccgctgtcg tgaaccatct cggcgtgcaa ggcgcggttc atgtcggcca ctccaccggc ggtggcgaag tcattcacta cgtcgcccgt catggcgaag accgcgtctc gaaggccgtg ctgatcagcg ctgtgccgcc gctgatggtc aagacagaca agaacccggg cggcctgccg aaggacgtgt tcgataacct gcaggcgcaa ctggccgcga accgtgctca gttttattac gacgttccgg ccggcccgtt ctacggctac aaccgtgccg gcgcgaaacc gtcgcaaggc gtgatctgga actggtggcg ccaaggcatg atgggcagcg ccaaggcgca ttacgacggc atcgtcgcgt tctcgcagac cgactttacc gaggacctga agagcaccac gattcccgtg ctcgtgatgc atggtgatga cgatcagatc gtcccctatg ccgattccgg cttgctgtcg gcgaagctcg ctcggaacag cacgctgaag acctacaagg gcttcccgca cggcatgcct acgtccaacg ccgacacgat caacgccgat ctgctcgcgt tcctgcgagg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat