BuceA.00095.a
Putative hydrolase
| CENTER ID: | BuceA.00095.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00095.a.B2.GE36729 | Mature protein( BuceA.00095.a ) | 28 | 302 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00095.a.B2.PW37307 | Mature protein( BuceA.00095.a ) | 28 | 302 |
Structures
4X00
DEPOSITED: 11/20/2014
DETERMINATION: XRay
CLONE: BuceA.00095.a.B2.GE36729
PROTEIN: BuceA.00095.a.B2.PW37307
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_187334 |
| PATRIC ID: | fig|216591.5.peg.2642 |
| RefSeq: | YP_002231650.1 |
| UniProt: | B4E794 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLSKLRGAL GALIATFALL AAAPATFAAA RDYTVTAPDG VVLAVQEAGD PEGSPIIFIH GLLGSRLNWS KQLQDPRLQH YRLITYDLRG HGLSGKPAEA SSYTDGRRWA DDLAAIIEST HARKPVLVGW SLGGAVISNY LAAYGDKGIA GAVYVDGVIE LKPDQIVAHP EVYRDMIASD LQTHLDGERA FLRLCFHRQP DATTFSLLLA NAALASWDMQ RAVRSMTVEA AKGLSKAEVP LLLLYGAQDA LVKAKPSIAR AKSLNPRIRS ELYADSGHAP FLEEPERFNR DLSDFVRMAL SR
NT Sequence
ATGTCTTTGT CGAAGCTTCG TGGTGCACTC GGCGCACTGA TAGCGACATT CGCGCTACTA GCCGCCGCAC CGGCTACGTT CGCCGCAGCC CGGGATTACA CCGTCACTGC CCCCGATGGA GTGGTGCTGG CCGTCCAGGA AGCTGGTGAT CCCGAGGGTT CTCCCATCAT TTTCATTCAT GGCCTGCTGG GTAGCCGGCT GAACTGGAGT AAGCAATTAC AGGACCCTCG ACTCCAGCAT TACCGATTGA TCACTTACGA CCTGCGCGGG CACGGCCTGT CGGGCAAGCC GGCCGAGGCC AGCTCCTACA CCGACGGACG CCGCTGGGCC GATGACCTGG CCGCGATCAT CGAATCGACA CACGCGCGCA AGCCGGTGCT CGTGGGATGG TCGCTGGGCG GTGCCGTGAT ATCGAACTAC CTGGCGGCCT ATGGCGACAA AGGTATCGCC GGCGCCGTCT ATGTCGACGG AGTGATCGAA CTGAAGCCCG ACCAGATCGT GGCGCACCCA GAAGTCTATC GCGACATGAT CGCTTCGGAC TTGCAAACCC ACTTGGACGG CGAGCGCGCT TTCCTGAGGC TGTGCTTCCA CCGCCAGCCC GACGCGACCA CGTTCTCCTT GCTGCTGGCC AACGCCGCGC TGGCATCGTG GGACATGCAA CGTGCCGTGC GGTCGATGAC GGTCGAGGCA GCCAAAGGAT TGAGCAAGGC CGAGGTTCCG CTCCTGCTGC TCTACGGCGC CCAGGACGCG CTCGTGAAGG CCAAGCCCAG TATCGCCAGG GCCAAGAGCC TCAACCCACG AATCCGCAGC GAGCTGTATG CCGATTCGGG ACACGCGCCA TTCCTCGAAG AACCGGAGCG CTTCAACCGG GACCTGTCGG ATTTCGTTCG CATGGCTCTT TCACGC
Details for BuceA.00095.a.B2.GE36729
| HARVESTED ON: | 2/27/2013 |
| SEQUENCED ON: | 2/2/2013 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA AARDYTVTAP DGVVLAVQEA GDPEGSPIIF IHGLLGSRLN WSKQLQDPRL QHYRLITYDL RGHGLSGKPA EASSYTDGRR WADDLAAIIE STHARKPVLV GWSLGGAVIS NYLAAYGDKG IAGAVYVDGV IELKPDQIVA HPEVYRDMIA SDLQTHLDGE RAFLRLCFHR QPDATTFSLL LANAALASWD MQRAVRSMTV EAAKGLSKAE VPLLLLYGAQ DALVKAKPSI ARAKSLNPRI RSELYADSGH APFLEEPERF NRDLSDFVRM ALSR
Validated NT Sequence
tcttactcac ttagcgtgaa agagccatgc gaacgaaatc cgacaggtcc cggttgaagc gctccggttc ttcgaggaat ggcgcgtgtc ccgaatcggc atacagctcg ctgcggattc gtgggttgag gctcttggcc ctggcgatac tgggcttggc cttcacgagc gcgtcctggg cgccgtagag cagcaggagc ggaacctcgg ccttgctcaa tcctttggct gcctcgaccg tcatcgaccg cacggcacgt tgcatgtccc acgatgccag cgcggcgttg gccagcagca aggagaacgt ggtcgcgtcg ggctggcggt ggaagcacag cctcaggaaa gcgcgctcgc cgtccaagtg ggtttgcaag tccgaagcga tcatgtcgcg atagacttct gggtgcgcca cgatctggtc gggcttcagt tcgatcactc cgtcgacata gacggcgccg gcgatacctt tgtcgccata ggccgccagg tagttcgata tcacggcacc gcccagcgac catcccacga gcaccggctt gcgcgcgtgt gtcgattcga tgatcgcggc caggtcatcg gcccagcggc gtccgtcggt gtaggagctg gcctcggccg gcttgcccga caggccgtgc ccgcgcaggt cgtaagtgat caatcggtaa tgctggagtc gagggtcctg taattgctta ctccagttca gccggctacc cagcaggcca tgaatgaaaa tgatgggaga accctcggga tcaccagctt cctggacggc cagcaccact ccatcggggg cagtgacggt gtaatcccgg gctgcggcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHAA ARDYTVTAPD GVVLAVQEAG DPEGSPIIFI HGLLGSRLNW SKQLQDPRLQ HYRLITYDLR GHGLSGKPAE ASSYTDGRRW ADDLAAIIES THARKPVLVG WSLGGAVISN YLAAYGDKGI AGAVYVDGVI ELKPDQIVAH PEVYRDMIAS DLQTHLDGER AFLRLCFHRQ PDATTFSLLL ANAALASWDM QRAVRSMTVE AAKGLSKAEV PLLLLYGAQD ALVKAKPSIA RAKSLNPRIR SELYADSGHA PFLEEPERFN RDLSDFVRMA LSR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgcag cccgggatta caccgtcact gcccccgatg gagtggtgct ggccgtccag gaagctggtg atcccgaggg ttctcccatc attttcattc atggcctgct gggtagccgg ctgaactgga gtaagcaatt acaggaccct cgactccagc attaccgatt gatcacttac gacctgcgcg ggcacggcct gtcgggcaag ccggccgagg ccagctccta caccgacgga cgccgctggg ccgatgacct ggccgcgatc atcgaatcga cacacgcgcg caagccggtg ctcgtgggat ggtcgctggg cggtgccgtg atatcgaact acctggcggc ctatggcgac aaaggtatcg ccggcgccgt ctatgtcgac ggagtgatcg aactgaagcc cgaccagatc gtggcgcacc cagaagtcta tcgcgacatg atcgcttcgg acttgcaaac ccacttggac ggcgagcgcg ctttcctgag gctgtgcttc caccgccagc ccgacgcgac cacgttctcc ttgctgctgg ccaacgccgc gctggcatcg tgggacatgc aacgtgccgt gcggtcgatg acggtcgagg cagccaaagg attgagcaag gccgaggttc cgctcctgct gctctacggc gcccaggacg cgctcgtgaa ggccaagccc agtatcgcca gggccaagag cctcaaccca cgaatccgca gcgagctgta tgccgattcg ggacacgcgc cattcctcga agaaccggag cgcttcaacc gggacctgtc ggatttcgtt cgcatggctc tttcacgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuceA.00095.a.B2.PW37307
| PURIFICATION DATe: | 6/10/2013 |
| CONCENTRATION: | 26.4mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA AARDYTVTAP DGVVLAVQEA GDPEGSPIIF IHGLLGSRLN WSKQLQDPRL QHYRLITYDL RGHGLSGKPA EASSYTDGRR WADDLAAIIE STHARKPVLV GWSLGGAVIS NYLAAYGDKG IAGAVYVDGV IELKPDQIVA HPEVYRDMIA SDLQTHLDGE RAFLRLCFHR QPDATTFSLL LANAALASWD MQRAVRSMTV EAAKGLSKAE VPLLLLYGAQ DALVKAKPSI ARAKSLNPRI RSELYADSGH APFLEEPERF NRDLSDFVRM ALSR
Validated NT Sequence
tcttactcac ttagcgtgaa agagccatgc gaacgaaatc cgacaggtcc cggttgaagc gctccggttc ttcgaggaat ggcgcgtgtc ccgaatcggc atacagctcg ctgcggattc gtgggttgag gctcttggcc ctggcgatac tgggcttggc cttcacgagc gcgtcctggg cgccgtagag cagcaggagc ggaacctcgg ccttgctcaa tcctttggct gcctcgaccg tcatcgaccg cacggcacgt tgcatgtccc acgatgccag cgcggcgttg gccagcagca aggagaacgt ggtcgcgtcg ggctggcggt ggaagcacag cctcaggaaa gcgcgctcgc cgtccaagtg ggtttgcaag tccgaagcga tcatgtcgcg atagacttct gggtgcgcca cgatctggtc gggcttcagt tcgatcactc cgtcgacata gacggcgccg gcgatacctt tgtcgccata ggccgccagg tagttcgata tcacggcacc gcccagcgac catcccacga gcaccggctt gcgcgcgtgt gtcgattcga tgatcgcggc caggtcatcg gcccagcggc gtccgtcggt gtaggagctg gcctcggccg gcttgcccga caggccgtgc ccgcgcaggt cgtaagtgat caatcggtaa tgctggagtc gagggtcctg taattgctta ctccagttca gccggctacc cagcaggcca tgaatgaaaa tgatgggaga accctcggga tcaccagctt cctggacggc cagcaccact ccatcggggg cagtgacggt gtaatcccgg gctgcggcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHAA ARDYTVTAPD GVVLAVQEAG DPEGSPIIFI HGLLGSRLNW SKQLQDPRLQ HYRLITYDLR GHGLSGKPAE ASSYTDGRRW ADDLAAIIES THARKPVLVG WSLGGAVISN YLAAYGDKGI AGAVYVDGVI ELKPDQIVAH PEVYRDMIAS DLQTHLDGER AFLRLCFHRQ PDATTFSLLL ANAALASWDM QRAVRSMTVE AAKGLSKAEV PLLLLYGAQD ALVKAKPSIA RAKSLNPRIR SELYADSGHA PFLEEPERFN RDLSDFVRMA LSR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGCAG CCCGGGATTA CACCGTCACT GCCCCCGATG GAGTGGTGCT GGCCGTCCAG GAAGCTGGTG ATCCCGAGGG TTCTCCCATC ATTTTCATTC ATGGCCTGCT GGGTAGCCGG CTGAACTGGA GTAAGCAATT ACAGGACCCT CGACTCCAGC ATTACCGATT GATCACTTAC GACCTGCGCG GGCACGGCCT GTCGGGCAAG CCGGCCGAGG CCAGCTCCTA CACCGACGGA CGCCGCTGGG CCGATGACCT GGCCGCGATC ATCGAATCGA CACACGCGCG CAAGCCGGTG CTCGTGGGAT GGTCGCTGGG CGGTGCCGTG ATATCGAACT ACCTGGCGGC CTATGGCGAC AAAGGTATCG CCGGCGCCGT CTATGTCGAC GGAGTGATCG AACTGAAGCC CGACCAGATC GTGGCGCACC CAGAAGTCTA TCGCGACATG ATCGCTTCGG ACTTGCAAAC CCACTTGGAC GGCGAGCGCG CTTTCCTGAG GCTGTGCTTC CACCGCCAGC CCGACGCGAC CACGTTCTCC TTGCTGCTGG CCAACGCCGC GCTGGCATCG TGGGACATGC AACGTGCCGT GCGGTCGATG ACGGTCGAGG CAGCCAAAGG ATTGAGCAAG GCCGAGGTTC CGCTCCTGCT GCTCTACGGC GCCCAGGACG CGCTCGTGAA GGCCAAGCCC AGTATCGCCA GGGCCAAGAG CCTCAACCCA CGAATCCGCA GCGAGCTGTA TGCCGATTCG GGACACGCGC CATTCCTCGA AGAACCGGAG CGCTTCAACC GGGACCTGTC GGATTTCGTT CGCATGGCTC TTTCACGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT