SSGCID: Target Page

BuceA.00094.a

Putative acetylglutamate kinase

CENTER ID:	BuceA.00094.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00094.a.B1.GE36728	Full length( BuceA.00094.a )		1	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.4231
RefSeq:	YP_002233167.1
UniProt:	B4EKI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFPDPDTPTR RVAGLARSLP FMQALHGQTV VIVDGGAASD VHTRSAFAQD VALLALTGIH PIVIQSIPTA SGAQSVHATH AAMAGVNHEL VRLIGSHGAR AIGVDGHDGG LLVASAESGH ANTSEVARFD VTALNAFLDN GLVPVVMPIA PDDAGRDCLL RPERLGSVLA QHTCAVSLVM MVDKGLLREL GELAGLYGIT ELEQWLAEHP AADAAPCVRD ALDALAHGVQ NVHLADIGQP ESLIDELLTD EGSGVVFCRR GNTDLLSEIR RYFADSACVL RDGFSVERKP VVRF

NT Sequence

ATGTTCCCAG ACCCTGACAC TCCCACTCGA CGCGTGGCCG GCTTGGCCCG CTCACTGCCG TTCATGCAGG CGCTGCACGG ACAGACCGTC GTCATCGTCG ACGGTGGTGC CGCCAGCGAC GTCCACACGC GCTCGGCCTT CGCGCAGGAT GTCGCGCTGC TCGCGCTCAC CGGCATCCAT CCGATCGTGA TCCAAAGCAT TCCGACTGCG TCCGGCGCGC AATCGGTCCA CGCGACACAC GCCGCCATGG CCGGCGTCAA CCATGAACTC GTGCGCCTGA TCGGCAGCCA CGGCGCGCGG GCAATCGGCG TCGACGGTCA CGACGGCGGC CTGCTGGTCG CCAGCGCCGA ATCCGGCCAC GCGAACACGA GCGAGGTCGC GCGGTTCGAC GTCACCGCAT TGAATGCGTT TCTCGACAAT GGACTGGTGC CCGTCGTGAT GCCCATCGCA CCAGACGACG CGGGCCGCGA CTGCCTGCTG CGCCCTGAAC GGCTCGGCAG CGTGCTCGCG CAACACACGT GCGCCGTCTC GTTGGTGATG ATGGTCGACA AAGGGTTGCT GCGCGAACTC GGCGAGCTGG CCGGGCTGTA CGGCATCACG GAGCTTGAGC AATGGCTCGC CGAGCATCCG GCCGCCGATG CGGCGCCGTG CGTGCGGGAC GCGCTCGATG CGCTCGCGCA CGGCGTGCAG AACGTCCATC TCGCCGATAT CGGGCAACCC GAATCGTTGA TCGACGAACT GTTGACGGAC GAAGGATCGG GCGTGGTGTT CTGCCGGCGC GGCAACACCG ATCTGCTGTC GGAAATCCGT CGTTACTTCG CCGATTCGGC GTGCGTGCTG CGCGACGGGT TCAGCGTCGA GCGGAAGCCG GTCGTGCGGT TT

Details for BuceA.00094.a.B1.GE36728

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	33kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMF PDPDTPTRRV AGLARSLPFM QALHGQTVVI VDGGAASDVH TRSAFAQDVA LLALTGIHPI VIQSIPTASG AQSVHATHAA MAGVNHELVR LIGSHGARAI GVDGHDGGLL VASAESGHAN TSEVARFDVT ALNAFLDNGL VPVVMPIAPD DAGRDCLLRP ERLGSVLAQH TCAVSLVMMV DKGLLRELGE LAGLYGITEL EQWLAEHPAA DAAPCVRDAL DALAHGVQNV HLADIGQPES LIDELLTDEG SGVVFCRRGN TDLLSEIRRY FADSACVLRD GFSVERKPVV RF

Validated NT Sequence

tactcactta aaaccgcacg accggcttcc gctcgacgct gaacccgtcg cgcagcacgc acgccgaatc ggcgaagtaa cgacggattt ccgacagcag atcggtgttg ccgcgccggc agaacaccac gcccgatcct tcgtccgtca acagttcgtc gatcaacgat tcgggttgcc cgatatcggc gagatggacg ttctgcacgc cgtgcgcgag cgcatcgagc gcgtcccgca cgcacggcgc cgcatcggcg gccggatgct cggcgagcca ttgctcaagc tccgtgatgc cgtacagccc ggccagctcg ccgagttcgc gcagcaaccc tttgtcgacc atcatcacca acgagacggc gcacgtgtgt tgcgcgagca cgctgccgag ccgttcaggg cgcagcaggc agtcgcggcc cgcgtcgtct ggtgcgatgg gcatcacgac gggcaccagt ccattgtcga gaaacgcatt caatgcggtg acgtcgaacc gcgcgacctc gctcgtgttc gcgtggccgg attcggcgct ggcgaccagc aggccgccgt cgtgaccgtc gacgccgatt gcccgcgcgc cgtggctgcc gatcaggcgc acgagttcat ggttgacgcc ggccatggcg gcgtgtgtcg cgtggaccga ttgcgcgccg gacgcagtcg gaatgctttg gatcacgatc ggatggatgc cggtgagcgc gagcagcgcg acatcctgcg cgaaggccga gcgcgtgtgg acgtcgctgg cggcaccacc gtcgacgatg acgacggtct gtccgtgcag cgcctgcatg aacggcagtg agcgggccaa gccggccacg cgtcgagtgg gagtgtcagg gtctgggaac atatggtggt ggtggtggtg agccatggta tntctcc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcc cagaccctga cactcccact cgacgcgtgg ccggcttggc ccgctcactg ccgttcatgc aggcgctgca cggacagacc gtcgtcatcg tcgacggtgg tgccgccagc gacgtccaca cgcgctcggc cttcgcgcag gatgtcgcgc tgctcgcgct caccggcatc catccgatcg tgatccaaag cattccgact gcgtccggcg cgcaatcggt ccacgcgaca cacgccgcca tggccggcgt caaccatgaa ctcgtgcgcc tgatcggcag ccacggcgcg cgggcaatcg gcgtcgacgg tcacgacggc ggcctgctgg tcgccagcgc cgaatccggc cacgcgaaca cgagcgaggt cgcgcggttc gacgtcaccg cattgaatgc gtttctcgac aatggactgg tgcccgtcgt gatgcccatc gcaccagacg acgcgggccg cgactgcctg ctgcgccctg aacggctcgg cagcgtgctc gcgcaacaca cgtgcgccgt ctcgttggtg atgatggtcg acaaagggtt gctgcgcgaa ctcggcgagc tggccgggct gtacggcatc acggagcttg agcaatggct cgccgagcat ccggccgccg atgcggcgcc gtgcgtgcgg gacgcgctcg atgcgctcgc gcacggcgtg cagaacgtcc atctcgccga tatcgggcaa cccgaatcgt tgatcgacga actgttgacg gacgaaggat cgggcgtggt gttctgccgg cgcggcaaca ccgatctgct gtcggaaatc cgtcgttact tcgccgattc ggcgtgcgtg ctgcgcgacg ggttcagcgt cgagcggaag ccggtcgtgc ggttttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat