BuceA.00093.c

Putative NADH:flavin oxidoreductase/NADH oxidase

CENTER ID: BuceA.00093.c
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00093.c.B1.GE36727 Full length( BuceA.00093.c ) 1 371
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6425
RefSeq: YP_002235210.1
UniProt: B4EK93

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQDPLFQPL QFGTLTLPNR IVMPPMTRSR ASQPGDEANE LMAAYYAQRA SAGLIVSEGT YIAPLGKGYA WTPGIHTPSQ VAGWRKVTDA VHAAHGRIFA QLWHVGRLSH TSLLGGQQPV SSSPLQAKGV NVFVAGEDGS TPGFVPASEP RALSIDEIRE IVDQYRAAAR HAIEAGFDGV ELHGANGYLV NQFIDSNANT RTDAYGGSLE NRLRFLREVT QALIDGTGDA SRVGIRLAPL TTLNGCVDDD PETTYLAAAK LLGELGVGYL HIAEADWDDA PLMPVAFKQQ LRAAFPGVLI YAGAYTAERA REAIAAGWAD LVAFGRPFVA NPDLPERLRT GAALAPHDRN TLFGGGARGL TDYPTLAQAA A
NT Sequence
ATGACCCAGG ACCCGCTGTT CCAACCGCTG CAATTCGGTA CGCTGACGCT GCCGAACCGC ATCGTGATGC CGCCGATGAC GCGCTCGCGC GCCAGCCAGC CCGGCGACGA AGCCAACGAA CTGATGGCCG CGTACTACGC GCAGCGCGCG AGTGCGGGCC TGATCGTCAG CGAAGGCACC TACATCGCGC CGCTCGGCAA GGGCTACGCA TGGACGCCCG GCATTCACAC GCCGTCGCAG GTCGCCGGCT GGCGCAAGGT GACGGACGCC GTGCATGCCG CGCACGGCCG CATCTTCGCG CAGCTGTGGC ACGTCGGCCG GCTGAGCCAT ACGAGCCTGC TCGGCGGCCA GCAACCGGTA TCGTCGTCGC CGCTCCAGGC GAAGGGCGTG AACGTGTTCG TCGCCGGTGA AGACGGCAGC ACGCCGGGCT TCGTGCCGGC GTCCGAGCCG CGCGCACTGT CGATCGACGA GATCCGCGAG ATCGTCGACC AGTACCGCGC CGCCGCCCGC CATGCGATCG AAGCGGGCTT CGACGGCGTC GAGCTGCACG GCGCGAACGG CTACCTCGTG AACCAGTTCA TCGACTCGAA CGCGAACACG CGTACCGATG CGTACGGCGG CTCGCTGGAA AACCGGCTGC GGTTCCTGCG CGAAGTCACG CAGGCGCTGA TCGACGGCAC CGGCGACGCG TCGCGCGTCG GCATCCGCCT CGCGCCGTTG ACCACGCTGA ACGGCTGCGT CGATGACGAT CCGGAAACCA CCTATCTCGC CGCCGCGAAG CTGCTCGGCG AACTCGGCGT CGGCTACCTG CACATCGCGG AAGCCGACTG GGACGACGCG CCGCTGATGC CGGTCGCCTT CAAGCAGCAA CTGCGCGCCG CGTTCCCGGG TGTGCTGATC TATGCCGGCG CGTACACCGC CGAGCGCGCC CGCGAAGCGA TCGCCGCCGG CTGGGCCGAC CTCGTCGCCT TCGGCCGCCC GTTCGTCGCG AACCCCGACC TGCCCGAGCG GCTGCGCACC GGCGCCGCGC TCGCGCCGCA CGATCGCAAC ACGCTGTTCG GCGGGGGCGC CCGAGGCCTG ACCGACTATC CGACGCTCGC GCAAGCCGCG GCG
Details for BuceA.00093.c.B1.GE36727
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 73
Validated AA Sequence
MPPMTRSRAS QPGDEANELM AAYYAQRASA GLIVSEGTYI APLGKGYAWT PGIHTPSQVA GWRKVTDAVH AAHGRIFAQL WHVGRLSHTS LLGGQQPVSS SPLQAKGVNV FVAGEDGSTP GFVPASEPRA LSIDEIREIV DQYRAAARHA IEAGFDGVEL HGANGYLVNQ FIDSNANTRT DAYGGSLENR LRFLREVTQA LIDGTGDASR VGIRLAPLTT LNGCVDDDPE TTYLAAAKLL GELGVGYLHI AEADWDXAPL MPVAFKQQXR AAFPGVXIY
Validated NT Sequence
catagatcnn cacaccnggg aacgcggcgc gcanttgctg cttgaaggcg accggcatca gcggcgcgtn gtcccagtcg gcttccgcga tgtgcaggta gccgacgccg agttcgccga gcagcttcgc ggcggcgaga taggtggttt ccggatcgtc atcgacgcag ccgttcagcg tggtcaacgg cgcgaggcgg atgccgacgc gcgacgcgtc gccggtgccg tcgatcagcg cctgcgtgac ttcgcgcagg aaccgcagcc ggttttccag cgagccgccg tacgcatcgg tacgcgtgtt cgcgttcgag tcgatgaact ggttcacgag gtagccgttc gcgccgtgca gctcgacgcc gtcgaagccc gcttcgatcg catggcgggc ggcggcgcgg tactggtcga cgatctcgcg gatctcgtcg atcgacagtg cgcgcggctc ggacgccggc acgaagcccg gcgtgctgcc gtcttcaccg gcgacgaaca cgttcacgcc cttcgcctgg agcggcgacg acgataccgg ttgctggccg ccgagcaggc tcgtatggct cagccggccg acgtgccaca gctgcgcgaa gatgcggccg tgcgcggcat gcacggcgtc cgtcaccttg cgccagccgg cgacctgcga cggcgtgtga atgccgggcg tccatgcgta gcccttgccg agcggcgcga tgtaggtgcc ttcgctgacg atcaggcccg cactcgcgcg ctgcgcgtag tacgcggcca tcagttcgtt ggcttcgtcg ccgggctggc tggcgcgcga gcgcgtcatc ggcggcatca cgatgcggtt cggcagcgtc agcgtaccga attgcagcgg ttggaacagc gggtcc
Expected Protein Sequence
MAHHHHHHMT QDPLFQPLQF GTLTLPNRIV MPPMTRSRAS QPGDEANELM AAYYAQRASA GLIVSEGTYI APLGKGYAWT PGIHTPSQVA GWRKVTDAVH AAHGRIFAQL WHVGRLSHTS LLGGQQPVSS SPLQAKGVNV FVAGEDGSTP GFVPASEPRA LSIDEIREIV DQYRAAARHA IEAGFDGVEL HGANGYLVNQ FIDSNANTRT DAYGGSLENR LRFLREVTQA LIDGTGDASR VGIRLAPLTT LNGCVDDDPE TTYLAAAKLL GELGVGYLHI AEADWDDAPL MPVAFKQQLR AAFPGVLIYA GAYTAERARE AIAAGWADLV AFGRPFVANP DLPERLRTGA ALAPHDRNTL FGGGARGLTD YPTLAQAAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc aggacccgct gttccaaccg ctgcaattcg gtacgctgac gctgccgaac cgcatcgtga tgccgccgat gacgcgctcg cgcgccagcc agcccggcga cgaagccaac gaactgatgg ccgcgtacta cgcgcagcgc gcgagtgcgg gcctgatcgt cagcgaaggc acctacatcg cgccgctcgg caagggctac gcatggacgc ccggcattca cacgccgtcg caggtcgccg gctggcgcaa ggtgacggac gccgtgcatg ccgcgcacgg ccgcatcttc gcgcagctgt ggcacgtcgg ccggctgagc catacgagcc tgctcggcgg ccagcaaccg gtatcgtcgt cgccgctcca ggcgaagggc gtgaacgtgt tcgtcgccgg tgaagacggc agcacgccgg gcttcgtgcc ggcgtccgag ccgcgcgcac tgtcgatcga cgagatccgc gagatcgtcg accagtaccg cgccgccgcc cgccatgcga tcgaagcggg cttcgacggc gtcgagctgc acggcgcgaa cggctacctc gtgaaccagt tcatcgactc gaacgcgaac acgcgtaccg atgcgtacgg cggctcgctg gaaaaccggc tgcggttcct gcgcgaagtc acgcaggcgc tgatcgacgg caccggcgac gcgtcgcgcg tcggcatccg cctcgcgccg ttgaccacgc tgaacggctg cgtcgatgac gatccggaaa ccacctatct cgccgccgcg aagctgctcg gcgaactcgg cgtcggctac ctgcacatcg cggaagccga ctgggacgac gcgccgctga tgccggtcgc cttcaagcag caactgcgcg ccgcgttccc gggtgtgctg atctatgccg gcgcgtacac cgccgagcgc gcccgcgaag cgatcgccgc cggctgggcc gacctcgtcg ccttcggccg cccgttcgtc gcgaaccccg acctgcccga gcggctgcgc accggcgccg cgctcgcgcc gcacgatcgc aacacgctgt tcggcggggg cgcccgaggc ctgaccgact atccgacgct cgcgcaagcc gcggcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t