BuceA.00093.a

Putative NADH-dependent flavin oxidoreductase

CENTER ID:	BuceA.00093.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystal structure
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00093.a.B1.GE36725	Full length( BuceA.00093.a )		1	370

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00093.a.B1.PW37302	Full length( BuceA.00093.a )		1	370

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127243
PATRIC ID:	fig\|216591.5.peg.5
RefSeq:	YP_002229168.1
UniProt:	B4E561

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTALFSPFTL RGVTLPNRIV ISPMCQYSAE RGEATDWHMI HLGHLALSGA GLLCIEATAV EPDGRITHGD LGLWDDVTEA ALKPVLAAIR KHSPVRIAMQ LSHAGRKASS AAPWEGGQLV PVADGGWLPH GPSAVPHKDG ETPPLALDAA GLNRIREAFA ASAKRAARLG IDAIEVHAAH GYLLHQFLSP LANQRTDEYG GSRDNRMRFP LEIFEIVRAA FPEDQPVGVR VSATDWVEGG WELDDTIAFA HELKRRGCDW IDVSSGGVSP LQKIPLSPGY QVPFAQAVKR AVGMPTIAVG LINEPAHANR LIEAGDADFV AMARAMLYDP RWPWHAAAEL GAQVTAPPQY WRSQPREHKA LFGDVAFGQR

NT Sequence

ATGACTGCGC TGTTTTCTCC GTTCACGCTG CGCGGCGTGA CCCTTCCGAA CCGGATCGTG ATTTCCCCGA TGTGCCAGTA CTCGGCCGAA CGCGGCGAAG CGACCGACTG GCACATGATT CACCTCGGCC ATCTCGCGCT GTCGGGCGCG GGGCTGCTGT GCATCGAGGC GACCGCGGTG GAACCCGACG GGCGCATCAC GCATGGCGAC CTCGGCCTGT GGGACGACGT GACCGAAGCC GCGCTGAAGC CGGTGCTCGC CGCGATCCGC AAGCATTCGC CGGTCCGTAT CGCGATGCAG TTGTCGCATG CGGGGCGCAA GGCGTCGAGC GCGGCGCCCT GGGAGGGCGG CCAGCTCGTG CCGGTCGCCG ATGGCGGCTG GTTGCCGCAT GGACCGTCGG CCGTGCCGCA CAAGGACGGC GAGACGCCGC CGCTCGCGCT CGATGCCGCG GGCCTGAACC GCATCCGCGA AGCCTTCGCG GCGTCGGCGA AGCGTGCCGC GCGGCTCGGC ATCGATGCGA TCGAAGTACA CGCGGCGCAC GGCTACCTGC TGCATCAGTT CCTGTCGCCG CTCGCGAATC AGCGCACCGA CGAATACGGC GGCTCGCGCG ACAACCGGAT GCGGTTTCCG CTCGAGATTT TCGAGATCGT GCGCGCGGCG TTTCCGGAAG ACCAGCCGGT CGGCGTGCGC GTGTCCGCGA CCGACTGGGT CGAAGGCGGC TGGGAGCTCG ACGATACGAT CGCGTTCGCG CACGAACTGA AGCGCCGCGG CTGCGACTGG ATCGACGTGT CGTCCGGCGG CGTGTCGCCG CTGCAGAAGA TTCCGCTGTC GCCCGGCTAC CAGGTGCCGT TCGCGCAGGC CGTGAAGCGC GCGGTCGGCA TGCCGACGAT CGCGGTCGGG CTGATCAACG AGCCCGCGCA CGCGAACCGG CTGATCGAGG CCGGCGATGC CGATTTCGTC GCGATGGCGC GCGCGATGCT GTACGACCCG CGCTGGCCGT GGCACGCGGC GGCCGAGCTC GGCGCGCAGG TGACGGCGCC GCCGCAATAC TGGCGCTCGC AGCCGCGCGA GCACAAGGCG CTGTTCGGCG ACGTCGCATT TGGCCAGCGC

Details for BuceA.00093.a.B1.GE36725

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	91

Validated AA Sequence

MCQYSAERGE ATDWHMIHLG HLALSGAGLL CIEATAVEPD GRITHGDLGL WDDVTEAALK PVLAAIRKHS PVRIAMQLSH AGRKASSAAP WEGGQLVPVA DGGWLPHGPS AVPHKDGETP PLALDAAGLN RIREAFAASA KRAARLGIDA IEVHAAHGYL LHQFLSPLAN QRTDEYGGSR DNRMRFPLEI FEIVRAAFPE DQPVGVRVSA TDWVEGGWEL DDTIAFAHEL KRRGCDWIDV SSGGVSPLQK IPLSPGYQVP FAQAVKRAVG MPTIAVGLIN EPAHANRLIE AGDADFVAMA RAMLYDPRWP WHAAAELGAQ VTAPPQYWRS QPREHKALFG DVAFGQR

Validated NT Sequence

atcttactca cttagcgctg gccaaatgcg acgtcgccga acagcgcctt gtgctcgcgc ggctgcgagc gccagtattg cggcggcgcc gtcacctgcg cgccgagctc ggccgccgcg tgccacggcc agcgcgggtc gtacagcatc gcgcgcgcca tcgcgacgaa atcggcatcg ccggcctcga tcagccggtt cgcgtgcgcg ggctcgttga tcagcccgac cgcgatcgtc ggcatgccga ccgcgcgctt cacggcctgc gcgaacggca cctggtagcc gggcgacagc ggaatcttct gcagcggcga cacgccgccg gacgacacgt cgatccagtc gcagccgcgg cgcttcagtt cgtgcgcgaa cgcgatcgta tcgtcgagct cccagccgcc ttcgacccag tcggtcgcgg acacgcgcac gccgaccggc tggtcttccg gaaacgccgc gcgcacgatc tcgaaaatct cgagcggaaa ccgcatccgg ttgtcgcgcg agccgccgta ttcgtcggtg cgctgattcg cgagcggcga caggaactga tgcagcaggt agccgtgcgc cgcgtgtact tcgatcgcat cgatgccgag ccgcgcggca cgcttcgccg acgccgcgaa ggcttcgcgg atgcggttca ggcccgcggc atcgagcgcg agcggcggcg tctcgccgtc cttgtgcggc acggccgacg gtccatgcgg caaccagccg ccatcggcga ccggcacgag ctggccgccc tcccagggcg ccgcgctcga cgccttgcgc cccgcatgcg acaactgcat cgcgatacgg accggcgaat gcttgcggat cgcggcgagc accggcttca gcgcggcttc ggtcacgtcg tcccacaggc cgaggtcgcc atgcgtgatg cgcccgtcgg gttccaccgc ggtcgcctcg atgcacagca gccccgcgcc cgacagcgcg agatggccga ggtgaatcat gtgccagtcg gtcgcttcgc cgcgttcggc cgagtactgg cacatcgggg aaatcacgat ccggttcgga agggtcacgc cgcgcagcgt gaacggagaa aacag

Expected Protein Sequence

MAHHHHHHMT ALFSPFTLRG VTLPNRIVIS PMCQYSAERG EATDWHMIHL GHLALSGAGL LCIEATAVEP DGRITHGDLG LWDDVTEAAL KPVLAAIRKH SPVRIAMQLS HAGRKASSAA PWEGGQLVPV ADGGWLPHGP SAVPHKDGET PPLALDAAGL NRIREAFAAS AKRAARLGID AIEVHAAHGY LLHQFLSPLA NQRTDEYGGS RDNRMRFPLE IFEIVRAAFP EDQPVGVRVS ATDWVEGGWE LDDTIAFAHE LKRRGCDWID VSSGGVSPLQ KIPLSPGYQV PFAQAVKRAV GMPTIAVGLI NEPAHANRLI EAGDADFVAM ARAMLYDPRW PWHAAAELGA QVTAPPQYWR SQPREHKALF GDVAFGQR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg cgctgttttc tccgttcacg ctgcgcggcg tgacccttcc gaaccggatc gtgatttccc cgatgtgcca gtactcggcc gaacgcggcg aagcgaccga ctggcacatg attcacctcg gccatctcgc gctgtcgggc gcggggctgc tgtgcatcga ggcgaccgcg gtggaacccg acgggcgcat cacgcatggc gacctcggcc tgtgggacga cgtgaccgaa gccgcgctga agccggtgct cgccgcgatc cgcaagcatt cgccggtccg tatcgcgatg cagttgtcgc atgcggggcg caaggcgtcg agcgcggcgc cctgggaggg cggccagctc gtgccggtcg ccgatggcgg ctggttgccg catggaccgt cggccgtgcc gcacaaggac ggcgagacgc cgccgctcgc gctcgatgcc gcgggcctga accgcatccg cgaagccttc gcggcgtcgg cgaagcgtgc cgcgcggctc ggcatcgatg cgatcgaagt acacgcggcg cacggctacc tgctgcatca gttcctgtcg ccgctcgcga atcagcgcac cgacgaatac ggcggctcgc gcgacaaccg gatgcggttt ccgctcgaga ttttcgagat cgtgcgcgcg gcgtttccgg aagaccagcc ggtcggcgtg cgcgtgtccg cgaccgactg ggtcgaaggc ggctgggagc tcgacgatac gatcgcgttc gcgcacgaac tgaagcgccg cggctgcgac tggatcgacg tgtcgtccgg cggcgtgtcg ccgctgcaga agattccgct gtcgcccggc taccaggtgc cgttcgcgca ggccgtgaag cgcgcggtcg gcatgccgac gatcgcggtc gggctgatca acgagcccgc gcacgcgaac cggctgatcg aggccggcga tgccgatttc gtcgcgatgg cgcgcgcgat gctgtacgac ccgcgctggc cgtggcacgc ggcggccgag ctcggcgcgc aggtgacggc gccgccgcaa tactggcgct cgcagccgcg cgagcacaag gcgctgttcg gcgacgtcgc atttggccag cgctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BuceA.00093.a.B1.PW37302

PURIFICATION DATe:	6/3/2013
CONCENTRATION:	52.2mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	91

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTG CGCTGTTTTC TCCGTTCACG CTGCGCGGCG TGACCCTTCC GAACCGGATC GTGATTTCCC CGATGTGCCA GTACTCGGCC GAACGCGGCG AAGCGACCGA CTGGCACATG ATTCACCTCG GCCATCTCGC GCTGTCGGGC GCGGGGCTGC TGTGCATCGA GGCGACCGCG GTGGAACCCG ACGGGCGCAT CACGCATGGC GACCTCGGCC TGTGGGACGA CGTGACCGAA GCCGCGCTGA AGCCGGTGCT CGCCGCGATC CGCAAGCATT CGCCGGTCCG TATCGCGATG CAGTTGTCGC ATGCGGGGCG CAAGGCGTCG AGCGCGGCGC CCTGGGAGGG CGGCCAGCTC GTGCCGGTCG CCGATGGCGG CTGGTTGCCG CATGGACCGT CGGCCGTGCC GCACAAGGAC GGCGAGACGC CGCCGCTCGC GCTCGATGCC GCGGGCCTGA ACCGCATCCG CGAAGCCTTC GCGGCGTCGG CGAAGCGTGC CGCGCGGCTC GGCATCGATG CGATCGAAGT ACACGCGGCG CACGGCTACC TGCTGCATCA GTTCCTGTCG CCGCTCGCGA ATCAGCGCAC CGACGAATAC GGCGGCTCGC GCGACAACCG GATGCGGTTT CCGCTCGAGA TTTTCGAGAT CGTGCGCGCG GCGTTTCCGG AAGACCAGCC GGTCGGCGTG CGCGTGTCCG CGACCGACTG GGTCGAAGGC GGCTGGGAGC TCGACGATAC GATCGCGTTC GCGCACGAAC TGAAGCGCCG CGGCTGCGAC TGGATCGACG TGTCGTCCGG CGGCGTGTCG CCGCTGCAGA AGATTCCGCT GTCGCCCGGC TACCAGGTGC CGTTCGCGCA GGCCGTGAAG CGCGCGGTCG GCATGCCGAC GATCGCGGTC GGGCTGATCA ACGAGCCCGC GCACGCGAAC CGGCTGATCG AGGCCGGCGA TGCCGATTTC GTCGCGATGG CGCGCGCGAT GCTGTACGAC CCGCGCTGGC CGTGGCACGC GGCGGCCGAG CTCGGCGCGC AGGTGACGGC GCCGCCGCAA TACTGGCGCT CGCAGCCGCG CGAGCACAAG GCGCTGTTCG GCGACGTCGC ATTTGGCCAG CGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT