BuceA.00092.d

Tartrate dehydrogenase

CENTER ID: BuceA.00092.d
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00092.d.B1.GE36724 Full length( BuceA.00092.d ) 1 367
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6978
RefSeq: YP_002153668.1
UniProt: B4EQ44

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNPHRIAVI AGDGIGKEVM PEGMRVLRAA ADRFDIPLAF DEFGDWCTDH YVRHGTMMPA DWARQIGAHD AIFFGAVGAP DIVPDHVAVW ESLIKIRREF DQYVNLRPVR LMPGVPAPLA GRRPGDIDFI VVRENTEGEY SSVGGRMFTG TDREIAVQQS VFSRVGVDRV LRFAFELARS RPRKQLTAAT KSNGISITMP FWDERLAEIA RSFPDIETRK YHIDILCAHF VQNPDRFDVV VASNLFGDIL SDLGPACTGT IGIAPSANLN PERRFPSLFE PVHGSAPDIA GRGIANPIGQ IWSAALLLQH LGRGESRYEA AAAEIVAAIE SVLVAGPRTR DMGGTASTAE LGAAIAHTIA NPASIEE
NT Sequence
ATGAAGAACC CGCACCGCAT CGCCGTCATC GCCGGCGACG GCATCGGCAA GGAAGTGATG CCCGAAGGCA TGCGCGTGTT GCGCGCTGCC GCCGACCGTT TCGACATTCC GCTCGCGTTC GACGAGTTCG GCGACTGGTG TACCGATCAC TACGTTCGCC ACGGCACGAT GATGCCGGCC GACTGGGCGC GGCAGATCGG CGCGCACGAC GCGATCTTCT TCGGCGCGGT TGGCGCGCCC GACATCGTGC CGGATCACGT CGCGGTATGG GAATCGCTGA TCAAGATCCG GCGCGAGTTC GATCAATACG TGAACCTGCG TCCGGTGCGC CTGATGCCGG GCGTGCCCGC GCCGCTCGCC GGACGCCGGC CCGGCGACAT CGATTTCATC GTCGTGCGCG AGAACACGGA AGGCGAATAT TCGTCGGTGG GCGGCCGGAT GTTCACCGGG ACCGACCGCG AAATCGCCGT GCAGCAGTCG GTTTTCTCGC GTGTCGGCGT CGACCGCGTG CTCCGGTTCG CGTTCGAGCT CGCCCGCTCG CGCCCGCGCA AGCAGCTGAC GGCCGCGACG AAATCCAACG GCATCTCGAT CACCATGCCG TTCTGGGACG AGCGCCTCGC GGAAATCGCG CGGTCGTTCC CGGACATCGA AACGCGCAAA TATCACATCG ACATACTGTG CGCACACTTC GTCCAGAATC CGGATCGCTT CGACGTCGTC GTCGCCTCCA ACCTGTTCGG CGACATCCTG TCCGACCTCG GCCCCGCCTG TACCGGCACG ATCGGCATCG CGCCGTCCGC GAACCTGAAC CCCGAGCGCC GCTTCCCGTC GCTGTTCGAG CCGGTGCACG GCTCGGCGCC CGACATCGCC GGCCGCGGCA TCGCCAATCC GATCGGGCAG ATCTGGTCGG CCGCGTTGCT GCTCCAGCAC CTCGGTCGCG GCGAATCGCG CTACGAAGCC GCGGCCGCCG AAATCGTCGC GGCGATCGAA TCCGTGCTCG TCGCCGGCCC GCGCACCCGC GACATGGGCG GCACGGCCAG CACCGCGGAA CTGGGCGCCG CGATAGCGCA CACCATCGCG AATCCCGCAT CGATCGAGGA A
Details for BuceA.00092.d.B1.GE36724
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 42kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 90
Validated AA Sequence
MPEGMRVLRA AADRFDIPLA FDEFGDWCTD HYVRHGTMMP ADWARQIGAH DAIFFGAVGA PDIVPDHVAV WESLIKIRRE FDQYVNLRPV RLMPGVPAPL AGRRPGDIDF IVVRENTEGE YSSVGGRMFT GTDREIAVQQ SVFSRVGVDR VLRFAFELAR SRPRKQLTAA TKSNGISITM PFWDERLAEI ARSFPDIETR KYHIDILCAH FVQNPDRFDV VVASNLFGDI LSDLGPACTG TIGIAPSANL NPERRFPSLF EPVHGSAPDI AGRGIANPIG QIWSAALLLQ HLGRGESRYE AAAAEIVAAI ESVLVAGPRT RDMGGTASTA ELGAAIAHT
Validated NT Sequence
atggtgtgcg ctatcgcggc gcccagttcc gcggtgctgg ccgtgccgcc catgtcgcgg gtgcgcgggc cggcgacgag cacggattcg atcgccgcga cgatttcggc ggccgcggct tcgtagcgcg attcgccgcg accgaggtgc tggagcagca acgcggccga ccagatctgc ccgatcggat tggcgatgcc gcggccggcg atgtcgggcg ccgagccgtg caccggctcg aacagcgacg ggaagcggcg ctcggggttc aggttcgcgg acggcgcgat gccgatcgtg ccggtacagg cggggccgag gtcggacagg atgtcgccga acaggttgga ggcgacgacg acgtcgaagc gatccggatt ctggacgaag tgtgcgcaca gtatgtcgat gtgatatttg cgcgtttcga tgtccgggaa cgaccgcgcg atttccgcga ggcgctcgtc ccagaacggc atggtgatcg agatgccgtt ggatttcgtc gcggccgtca gctgcttgcg cgggcgcgag cgggcgagct cgaacgcgaa ccggagcacg cggtcgacgc cgacacgcga gaaaaccgac tgctgcacgg cgatttcgcg gtcggtcccg gtgaacatcc ggccgcccac cgacgaatat tcgccttccg tgttctcgcg cacgacgatg aaatcgatgt cgccgggccg gcgtccggcg agcggcgcgg gcacgcccgg catcaggcgc accggacgca ggttcacgta ttgatcgaac tcgcgccgga tcttgatcag cgattcccat accgcgacgt gatccggcac gatgtcgggc gcgccaaccg cgccgaagaa gatcgcgtcg tgcgcgccga tctgccgcgc ccagtcggcc ggcatcatcg tgccgtggcg aacgtagtga tcggtacacc agtcgccgaa ctcgtcgaac gcgagcggaa tgtcgaaacg gtcggcggca gcgcgcaaca cgcgcatgcc ttcgggcatc acttccttgc cgatgccgtc gccggcgatg acggcgatgc ggtgcggg
Expected Protein Sequence
MAHHHHHHMK NPHRIAVIAG DGIGKEVMPE GMRVLRAAAD RFDIPLAFDE FGDWCTDHYV RHGTMMPADW ARQIGAHDAI FFGAVGAPDI VPDHVAVWES LIKIRREFDQ YVNLRPVRLM PGVPAPLAGR RPGDIDFIVV RENTEGEYSS VGGRMFTGTD REIAVQQSVF SRVGVDRVLR FAFELARSRP RKQLTAATKS NGISITMPFW DERLAEIARS FPDIETRKYH IDILCAHFVQ NPDRFDVVVA SNLFGDILSD LGPACTGTIG IAPSANLNPE RRFPSLFEPV HGSAPDIAGR GIANPIGQIW SAALLLQHLG RGESRYEAAA AEIVAAIESV LVAGPRTRDM GGTASTAELG AAIAHTIANP ASIEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga acccgcaccg catcgccgtc atcgccggcg acggcatcgg caaggaagtg atgcccgaag gcatgcgcgt gttgcgcgct gccgccgacc gtttcgacat tccgctcgcg ttcgacgagt tcggcgactg gtgtaccgat cactacgttc gccacggcac gatgatgccg gccgactggg cgcggcagat cggcgcgcac gacgcgatct tcttcggcgc ggttggcgcg cccgacatcg tgccggatca cgtcgcggta tgggaatcgc tgatcaagat ccggcgcgag ttcgatcaat acgtgaacct gcgtccggtg cgcctgatgc cgggcgtgcc cgcgccgctc gccggacgcc ggcccggcga catcgatttc atcgtcgtgc gcgagaacac ggaaggcgaa tattcgtcgg tgggcggccg gatgttcacc gggaccgacc gcgaaatcgc cgtgcagcag tcggttttct cgcgtgtcgg cgtcgaccgc gtgctccggt tcgcgttcga gctcgcccgc tcgcgcccgc gcaagcagct gacggccgcg acgaaatcca acggcatctc gatcaccatg ccgttctggg acgagcgcct cgcggaaatc gcgcggtcgt tcccggacat cgaaacgcgc aaatatcaca tcgacatact gtgcgcacac ttcgtccaga atccggatcg cttcgacgtc gtcgtcgcct ccaacctgtt cggcgacatc ctgtccgacc tcggccccgc ctgtaccggc acgatcggca tcgcgccgtc cgcgaacctg aaccccgagc gccgcttccc gtcgctgttc gagccggtgc acggctcggc gcccgacatc gccggccgcg gcatcgccaa tccgatcggg cagatctggt cggccgcgtt gctgctccag cacctcggtc gcggcgaatc gcgctacgaa gccgcggccg ccgaaatcgt cgcggcgatc gaatccgtgc tcgtcgccgg cccgcgcacc cgcgacatgg gcggcacggc cagcaccgcg gaactgggcg ccgcgatagc gcacaccatc gcgaatcccg catcgatcga ggaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat