BuceA.00092.c

Putative tartrate dehydrogenase

CENTER ID: BuceA.00092.c
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00092.c.B1.GE36723 Full length( BuceA.00092.c ) 1 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4447
RefSeq: YP_002233366.1
UniProt: B4EM45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTYRIATIP GDGIGKEVVP AGKQVLEALA RGSDRFAFEF EDFDWGADYY RRHGAMMPAD GLDALRGKDA ILFGSAGDPD VPDHVTLWGL RLKICQGFDQ YANVRPTRIL PGIDAPLKRC GPDDLNWVIV RENSEGEYAG VGGRVHQGHP IEAATDVSIL TRAGVERIMR FAFRLAQSRP RKLLTVITKS NAQRHAMVMW DEIAKQIAQE FPDVTWDKEL VDAATARMVN RPASLDTIVA TNLHADILSD LAAALAGSLG IAPTGNIDPE RRYPSMFEPI HGSAFDIMGK GLANPVGTFW SVVMLLEHLG ETEAAARVMQ AIEAVTADPS LHTRDLGGRA TTAQVTAAVC ERVANAAVAA
NT Sequence
ATGAAGACCT ACCGTATCGC AACGATTCCC GGCGACGGCA TCGGCAAGGA AGTGGTGCCG GCTGGCAAGC AGGTGCTGGA GGCGCTGGCC CGCGGCAGCG ACCGCTTCGC GTTCGAGTTC GAGGATTTCG ACTGGGGCGC CGATTACTAC CGCCGGCATG GCGCGATGAT GCCGGCCGAC GGTCTCGACG CGCTGCGCGG CAAGGACGCG ATCCTGTTCG GCTCCGCGGG CGATCCGGAC GTGCCCGACC ACGTGACGCT GTGGGGGCTG CGCCTGAAGA TCTGCCAGGG CTTCGACCAG TACGCGAACG TGCGTCCGAC GCGCATCCTG CCGGGCATCG ACGCGCCGCT GAAGCGCTGC GGGCCGGACG ACCTGAACTG GGTGATCGTG CGCGAGAACT CCGAAGGCGA ATACGCGGGC GTCGGCGGCC GCGTGCACCA GGGCCATCCG ATCGAGGCCG CGACCGATGT GTCGATCCTC ACGCGCGCCG GCGTCGAACG CATCATGCGT TTCGCATTCC GGCTCGCGCA GTCGCGCCCA CGCAAGCTGT TGACCGTCAT CACGAAGAGC AACGCGCAGC GGCATGCGAT GGTGATGTGG GACGAGATCG CGAAGCAGAT CGCACAGGAG TTTCCCGACG TGACGTGGGA CAAGGAACTC GTCGATGCAG CAACCGCGCG CATGGTCAAC CGCCCGGCGT CGCTCGACAC GATCGTCGCG ACCAACCTGC ACGCGGACAT CCTCAGCGAT CTTGCCGCCG CGCTCGCGGG CAGCCTCGGC ATCGCGCCGA CCGGCAACAT CGATCCCGAG CGCCGCTATC CGTCGATGTT CGAGCCAATC CACGGCTCCG CATTCGACAT CATGGGCAAG GGGCTCGCGA ATCCGGTCGG CACGTTCTGG TCGGTCGTGA TGCTGCTCGA GCATCTCGGC GAGACGGAAG CGGCCGCGCG TGTGATGCAG GCGATCGAGG CCGTGACGGC CGACCCGTCG CTGCATACGC GCGACCTCGG CGGCCGCGCG ACGACCGCGC AGGTGACGGC GGCCGTCTGC GAGCGCGTCG CGAACGCGGC GGTGGCGGCC
Details for BuceA.00092.c.B1.GE36723
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 41kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 22
PERCENT COVERAGE: 89
Validated AA Sequence
MQRRVGRHGL DRLHHTRGRF RLAEMLEQHH DRPERADRIR EPLAHDVECG AVDWLEHRRI AALGIDVAGR RDAEAARERG GKIAEDVRVQ VGRDDRVERR RAVDHARGCC IDEFLVPRHV GKLLCDLLRD LVPHHHRMPL RVALRDDGQQ LAWARLREPE CETHDAFDAG AREDRHIGRG LDRMALVHAA ADARVFAFGV LAHDHPVQVV RPAALQRRVD ARQDARRTHV RVLVEALADL QAQPPQRHVV GHVRIARGAE QDRVLAAQRV ETVGRHHRAM PAVVIGAPVE ILELEREAVA AAGQRLQHLL ASRHHFLADA VAGNRCDTV
Validated NT Sequence
cgctcgcaga cggccgccgt cacctgcgcg gtcgtcgcgc ggccgccgag gtcgcgcgta tgcagcgacg ggtcggccgt cacggcctcg atcgcctgca tcacacgcgc ggccgcttcc gtctcgccga gatgctcgag cagcatcacg accgaccaga acgtgccgac cggattcgcg agccccttgc ccatgatgtc gaatgcggag ccgtggattg gctcgaacat cgacggatag cggcgctcgg gatcgatgtt gccggtcggc gcgatgccga ggctgcccgc gagcgcggcg gcaagatcgc tgaggatgtc cgcgtgcagg ttggtcgcga cgatcgtgtc gagcgacgcc gggcggttga ccatgcgcgc ggttgctgca tcgacgagtt ccttgtccca cgtcacgtcg ggaaactcct gtgcgatctg cttcgcgatc tcgtcccaca tcaccatcgc atgccgctgc gcgttgctct tcgtgatgac ggtcaacagc ttgcgtgggc gcgactgcgc gagccggaat gcgaaacgca tgatgcgttc gacgccggcg cgcgtgagga tcgacacatc ggtcgcggcc tcgatcggat ggccctggtg cacgcggccg ccgacgcccg cgtattcgcc ttcggagttc tcgcgcacga tcacccagtt caggtcgtcc ggcccgcagc gcttcagcgg cgcgtcgatg cccggcagga tgcgcgtcgg acgcacgttc gcgtactggt cgaagccctg gcagatcttc aggcgcagcc cccacagcgt cacgtggtcg ggcacgtccg gatcgcccgc ggagccgaac aggatcgcgt ccttgccgcg cagcgcgtcg agaccgtcgg ccggcatcat cgcgccatgc cggcggtagt aatcggcgcc ccagtcgaaa tcctcgaact cgaacgcgaa gcggtcgctg ccgcgggcca gcgcctccag cacctgcttg ccagccggca ccacttcctt gccgatgccg tcgccgggaa tcgttgcgat acggtagg
Expected Protein Sequence
MAHHHHHHMK TYRIATIPGD GIGKEVVPAG KQVLEALARG SDRFAFEFED FDWGADYYRR HGAMMPADGL DALRGKDAIL FGSAGDPDVP DHVTLWGLRL KICQGFDQYA NVRPTRILPG IDAPLKRCGP DDLNWVIVRE NSEGEYAGVG GRVHQGHPIE AATDVSILTR AGVERIMRFA FRLAQSRPRK LLTVITKSNA QRHAMVMWDE IAKQIAQEFP DVTWDKELVD AATARMVNRP ASLDTIVATN LHADILSDLA AALAGSLGIA PTGNIDPERR YPSMFEPIHG SAFDIMGKGL ANPVGTFWSV VMLLEHLGET EAAARVMQAI EAVTADPSLH TRDLGGRATT AQVTAAVCER VANAAVAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga cctaccgtat cgcaacgatt cccggcgacg gcatcggcaa ggaagtggtg ccggctggca agcaggtgct ggaggcgctg gcccgcggca gcgaccgctt cgcgttcgag ttcgaggatt tcgactgggg cgccgattac taccgccggc atggcgcgat gatgccggcc gacggtctcg acgcgctgcg cggcaaggac gcgatcctgt tcggctccgc gggcgatccg gacgtgcccg accacgtgac gctgtggggg ctgcgcctga agatctgcca gggcttcgac cagtacgcga acgtgcgtcc gacgcgcatc ctgccgggca tcgacgcgcc gctgaagcgc tgcgggccgg acgacctgaa ctgggtgatc gtgcgcgaga actccgaagg cgaatacgcg ggcgtcggcg gccgcgtgca ccagggccat ccgatcgagg ccgcgaccga tgtgtcgatc ctcacgcgcg ccggcgtcga acgcatcatg cgtttcgcat tccggctcgc gcagtcgcgc ccacgcaagc tgttgaccgt catcacgaag agcaacgcgc agcggcatgc gatggtgatg tgggacgaga tcgcgaagca gatcgcacag gagtttcccg acgtgacgtg ggacaaggaa ctcgtcgatg cagcaaccgc gcgcatggtc aaccgcccgg cgtcgctcga cacgatcgtc gcgaccaacc tgcacgcgga catcctcagc gatcttgccg ccgcgctcgc gggcagcctc ggcatcgcgc cgaccggcaa catcgatccc gagcgccgct atccgtcgat gttcgagcca atccacggct ccgcattcga catcatgggc aaggggctcg cgaatccggt cggcacgttc tggtcggtcg tgatgctgct cgagcatctc ggcgagacgg aagcggccgc gcgtgtgatg caggcgatcg aggccgtgac ggccgacccg tcgctgcata cgcgcgacct cggcggccgc gcgacgaccg cgcaggtgac ggcggccgtc tgcgagcgcg tcgcgaacgc ggcggtggcg gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat