SSGCID: Target Page

BuceA.00092.a

3-isopropylmalate dehydrogenase

CENTER ID:	BuceA.00092.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00092.a.B1.GE36721	Full length( BuceA.00092.a )		1	355

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.4708
RefSeq:	YP_002233604.1
UniProt:	B4EFJ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIAVLPGDG IGPEIVNEAV KVLNALDETF ELEQAPVGGA GYEASGHPLP DATLALAKQA DAILFGAVGD WKYDSLERAL RPEQAILGLR KHLELFANFR PAICYPQLVD ASPLKPELVA GLDILIVREL NGDIYFGQPR GVRAAPDGPF AGAREGFDTM RYSEPEVRRI AHVAFQAAQK RAKKLLSVDK SNVLETSQFW RDIMIDVSKE YADVELSHMY VDNAAMQLAK APKQFDVIVT GNMFGDILSD EASMLTGSIG MLPSASLDKN NKGLYEPSHG SAPDIAGKGI ANPLATILSA AMLLRYSLNR AEQADRIERA VKTVLEQGYR TGDIATPGCK QVGTAAMGDA VVAAL

NT Sequence

ATGAAGATTG CAGTGCTGCC CGGCGACGGC ATCGGTCCGG AAATCGTCAA TGAAGCCGTG AAGGTGCTGA ACGCACTCGA CGAGACGTTC GAACTCGAAC AGGCGCCGGT CGGCGGCGCG GGCTACGAGG CGAGCGGCCA TCCGCTGCCC GACGCGACGC TGGCGCTCGC GAAGCAGGCC GACGCGATCC TGTTCGGCGC GGTCGGCGAC TGGAAGTACG ACTCGCTGGA GCGCGCGCTG CGCCCCGAGC AGGCGATCCT CGGGCTGCGC AAGCACCTCG AGCTGTTCGC GAACTTCCGC CCGGCGATCT GCTATCCGCA GCTCGTCGAT GCGTCGCCGT TGAAGCCGGA GCTCGTCGCG GGCCTCGACA TCCTGATCGT GCGCGAACTG AACGGCGACA TCTACTTCGG CCAGCCGCGC GGCGTGCGCG CGGCGCCGGA CGGCCCGTTC GCCGGCGCGC GCGAAGGCTT CGACACGATG CGCTATTCGG AGCCGGAAGT GCGCCGCATC GCGCACGTCG CGTTCCAGGC CGCGCAAAAG CGCGCGAAGA AGCTGCTGTC GGTCGACAAG TCGAACGTAC TGGAAACGTC GCAGTTCTGG CGCGACATCA TGATCGACGT GTCGAAGGAA TACGCGGACG TCGAGCTGTC GCACATGTAC GTCGACAACG CGGCGATGCA GCTCGCGAAG GCGCCGAAGC AGTTCGACGT GATCGTCACG GGCAACATGT TCGGCGACAT CCTGTCGGAT GAAGCGTCGA TGCTGACGGG TTCGATCGGC ATGCTGCCGT CGGCGTCGCT CGACAAGAAC AACAAGGGCC TGTACGAGCC GTCGCACGGT TCGGCGCCGG ACATCGCCGG CAAGGGCATC GCGAACCCGC TCGCGACGAT CCTGTCGGCC GCGATGCTGC TGCGCTACTC GCTGAATCGC GCCGAGCAGG CCGATCGCAT CGAGCGTGCG GTGAAAACGG TGCTCGAACA GGGTTATCGC ACGGGCGACA TCGCGACGCC GGGCTGCAAG CAGGTCGGCA CGGCGGCGAT GGGCGACGCG GTGGTCGCGG CGCTG

Details for BuceA.00092.a.B1.GE36721

HARVESTED ON:	2/27/2013
SEQUENCED ON:	2/2/2013
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	51

Validated AA Sequence

MRYSEPEVRR IAHVAFQAAQ KRAKKLLSVD KSNVLETSQF WRDIMIDVSK EYADVELSHM YVDNAAMQLA KAPKQFDVIV TGNMFGDILS DEASMLTGSI GMLPSASLDK NNKGLYEPSH GSAPDIAGKG IANPLATILS AAMLLRYSLN RAEQADRIER AVKTVLEQGY RTGDIATPGC KQVGTAA

Validated NT Sequence

tcgccgccgt gccgacctgc ttgcagcccg gcgtcgcgat gtcgcccgtg cgataaccct gttcgagcac cgttttcacc gcacgctcga tgcgatcggc ctgctcggcg cgattcagcg agtagcgcag cagcatcgcg gccgacagga tcgtcgcgag cgggttcgcg atgcccttgc cggcgatgtc cggcgccgaa ccgtgcgacg gctcgtacag gcccttgttg ttcttgtcga gcgacgccga cggcagcatg ccgatcgaac ccgtcagcat cgacgcttca tccgacagga tgtcgccgaa catgttgccc gtgacgatca cgtcgaactg cttcggcgcc ttcgcgagct gcatcgccgc gttgtcgacg tacatgtgcg acagctcgac gtccgcgtat tccttcgaca cgtcgatcat gatgtcgcgc cagaactgcg acgtttccag tacgttcgac ttgtcgaccg acagcagctt cttcgcgcgc ttttgcgcgg cctggaacgc gacgtgcgcg atgcggcgca cttccggctc cgaatagcgc atcgtgtcga agccttcgcg cgcgccggcg aacgggccgt ccggcgccgc gcgcacgccg cgcggctggc cgaagtagat gtcgccgttc agttcgcgca cgatcaggat gtcgaggccc gcgacgagct ccggcttcaa cggcgacgca tcgacgagct gcggatagca gatcgccggg cggaagttcg cgaacagctc gaggtgcttg cgcagcccga ggatcgcctg ctcggggcgc agcgcgcgct ccagcgagtc gtacttccag tcgccgaccg cgccgaacag gatcgcgtcg gcctgcttcg cgagcgccag cgtcgcgtcg ggcagcggat ggccgctcgc ctcgtagccc gcgccgccga ccggcgcctg ttcgagttcg aacgtctcgt cgagtgcgtt cagcaccttc acggcttcat tgacgatttc cggaccgatg ccgtcgccgg gcagcactgc aat

Expected Protein Sequence

MAHHHHHHMK IAVLPGDGIG PEIVNEAVKV LNALDETFEL EQAPVGGAGY EASGHPLPDA TLALAKQADA ILFGAVGDWK YDSLERALRP EQAILGLRKH LELFANFRPA ICYPQLVDAS PLKPELVAGL DILIVRELNG DIYFGQPRGV RAAPDGPFAG AREGFDTMRY SEPEVRRIAH VAFQAAQKRA KKLLSVDKSN VLETSQFWRD IMIDVSKEYA DVELSHMYVD NAAMQLAKAP KQFDVIVTGN MFGDILSDEA SMLTGSIGML PSASLDKNNK GLYEPSHGSA PDIAGKGIAN PLATILSAAM LLRYSLNRAE QADRIERAVK TVLEQGYRTG DIATPGCKQV GTAAMGDAVV AAL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga ttgcagtgct gcccggcgac ggcatcggtc cggaaatcgt caatgaagcc gtgaaggtgc tgaacgcact cgacgagacg ttcgaactcg aacaggcgcc ggtcggcggc gcgggctacg aggcgagcgg ccatccgctg cccgacgcga cgctggcgct cgcgaagcag gccgacgcga tcctgttcgg cgcggtcggc gactggaagt acgactcgct ggagcgcgcg ctgcgccccg agcaggcgat cctcgggctg cgcaagcacc tcgagctgtt cgcgaacttc cgcccggcga tctgctatcc gcagctcgtc gatgcgtcgc cgttgaagcc ggagctcgtc gcgggcctcg acatcctgat cgtgcgcgaa ctgaacggcg acatctactt cggccagccg cgcggcgtgc gcgcggcgcc ggacggcccg ttcgccggcg cgcgcgaagg cttcgacacg atgcgctatt cggagccgga agtgcgccgc atcgcgcacg tcgcgttcca ggccgcgcaa aagcgcgcga agaagctgct gtcggtcgac aagtcgaacg tactggaaac gtcgcagttc tggcgcgaca tcatgatcga cgtgtcgaag gaatacgcgg acgtcgagct gtcgcacatg tacgtcgaca acgcggcgat gcagctcgcg aaggcgccga agcagttcga cgtgatcgtc acgggcaaca tgttcggcga catcctgtcg gatgaagcgt cgatgctgac gggttcgatc ggcatgctgc cgtcggcgtc gctcgacaag aacaacaagg gcctgtacga gccgtcgcac ggttcggcgc cggacatcgc cggcaagggc atcgcgaacc cgctcgcgac gatcctgtcg gccgcgatgc tgctgcgcta ctcgctgaat cgcgccgagc aggccgatcg catcgagcgt gcggtgaaaa cggtgctcga acagggttat cgcacgggcg acatcgcgac gccgggctgc aagcaggtcg gcacggcggc gatgggcgac gcggtggtcg cggcgctgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat