BuceA.00090.b

Putative phospho-2-dehydro-3-deoxyheptonate aldolase, phe-sensitive

CENTER ID: BuceA.00090.b
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00090.b.B1.GE36720 Full length( BuceA.00090.b ) 1 376
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6595
RefSeq: YP_002235364.1
UniProt: B4ELG2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQNLDNPSHD REVGSADATQ DTTRIDDVRI GAVRPLISPA LLLDELPVPA ATQTLVEDTR RAIGDILHGR DDRLLLVVGP CSIHDHDQAL DYARRLKVAA DALKDDLLIT MRVYFEKPRT TVGWKGYIND PRLDGSFRIN EGLRAARQLL LDINALGLPA STEFLDLLSP QYIADLIAWG AIGARTTESQ SHRQLASGLS CPIGFKNGTD GGVQVAADAI VAAAASHAFM GMTKMGMAAI FETRGNDDAH VILRGGKTGP NYDAEHVEAT CAVLRKSGLR EQVMVDCSHA NSNKSHDRQV DVAQDLARQL SQGEHRIVGV MVESHLEAGR QDLKPGVPLQ YGVSITDACL SWTQTEPVLD VLAEAVRHRR VYSRNA
NT Sequence
ATGCAGAACC TCGACAATCC TTCCCACGAT CGCGAGGTAG GCAGCGCCGA CGCGACGCAG GACACCACCC GCATCGACGA CGTCCGCATC GGCGCCGTGC GTCCGCTGAT TTCGCCCGCG CTGCTGCTCG ACGAGCTGCC GGTGCCGGCC GCCACGCAGA CGCTCGTCGA GGATACGCGC CGCGCGATCG GCGACATCCT GCACGGCCGC GACGACCGGC TGCTGCTCGT CGTCGGCCCG TGCTCGATCC ACGATCACGA CCAGGCGCTC GATTACGCGC GCCGGCTGAA GGTCGCCGCC GACGCGCTCA AGGACGACCT GCTGATCACG ATGCGCGTGT ACTTCGAGAA GCCGCGTACG ACGGTCGGCT GGAAGGGGTA CATCAACGAT CCGCGCCTGG ATGGCAGCTT CCGCATCAAC GAAGGGCTGC GCGCCGCGCG GCAACTGCTG CTCGACATCA ACGCGCTCGG CCTGCCCGCA TCGACCGAAT TCCTCGACCT GCTGAGCCCG CAGTACATCG CCGACCTGAT CGCGTGGGGC GCGATCGGCG CGCGCACCAC CGAGAGCCAG AGCCATCGCC AGCTCGCGTC GGGGCTGAGC TGCCCGATCG GCTTCAAGAA CGGCACCGAC GGCGGCGTGC AGGTCGCGGC GGACGCGATC GTTGCTGCGG CGGCGAGCCA TGCGTTCATG GGGATGACGA AGATGGGGAT GGCCGCGATC TTCGAGACGC GCGGCAACGA CGACGCGCAC GTGATCCTGC GCGGCGGCAA GACCGGCCCG AACTACGACG CGGAGCATGT CGAGGCGACC TGCGCGGTGC TGCGCAAGAG CGGATTGCGC GAGCAGGTGA TGGTCGACTG CTCGCATGCG AATTCCAACA AGTCGCACGA CCGGCAGGTC GACGTCGCGC AGGATCTCGC ACGGCAACTG TCGCAGGGCG AGCACCGGAT CGTCGGCGTC ATGGTCGAGA GCCACCTCGA GGCCGGGCGG CAGGATCTGA AGCCGGGCGT GCCGCTGCAG TACGGCGTGT CGATCACCGA TGCATGCCTG AGCTGGACGC AGACGGAGCC GGTGCTCGAC GTGCTGGCGG AGGCGGTGCG GCATCGGCGG GTGTATTCGC GGAATGCG
Details for BuceA.00090.b.B1.GE36720
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 24
PERCENT COVERAGE: 74
Validated AA Sequence
MRAVDHHLLA QSALAQHRAG RLDMLRVVVR AGLAAAQDHV RVVVAARLED RGHPHLRHPH ERMARRRSND RVRRDLHAAV GAVLEADRAA QPRRELAMAL ALGGARADRA PRDQVGDVLR AQQVEEFGRC GQAERVDVEQ QLPRGAQPFV DAEAAIQARI VDVPLPADRR TRLLEVHAHR DQQVVLERVG GDLQPARVIE RLVVIVDRAR ADDEQQPVVA AVQDVADRAA RILDERLRGG RHRQLVEQQR GRNQRTHGAD ADVVDAGGVL RRVGAAYLAI VGRIVE
Validated NT Sequence
gccgcaccgc ctccgccagc acgtcgagca ccggctccgt ctgcgtccag ctcaggcatg catcggtgat cgacacgccg tactgcagcg gcacgcccgg cttcagatcc tgccgcccgg cctcgaggtg gctctcgacc atgacgccga cgatccggtg ctcgccctgc gacagttgcc gtgcgagatc ctgcgcgacg tcgacctgcc ggtcgtgcga cttgttggaa ttcgcatgcg agcagtcgac catcacctgc tcgcgcaatc cgctcttgcg cagcaccgcg caggtcgcct cgacatgctc cgcgtcgtag ttcgggccgg tcttgccgcc gcgcaggatc acgtgcgcgt cgtcgttgcc gcgcgtctcg aagatcgcgg ccatccccat cttcgtcatc cccatgaacg catggctcgc cgccgcagca acgatcgcgt ccgccgcgac ctgcacgccg ccgtcggtgc cgttcttgaa gccgatcggg cagctcagcc ccgacgcgag ctggcgatgg ctctggctct cggtggtgcg cgcgccgatc gcgccccacg cgatcaggtc ggcgatgtac tgcgggctca gcaggtcgag gaattcggtc gatgcgggca ggccgagcgc gttgatgtcg agcagcagtt gccgcgcggc gcgcagccct tcgttgatgc ggaagctgcc atccaggcgc ggatcgttga tgtacccctt ccagccgacc gtcgtacgcg gcttctcgaa gtacacgcgc atcgtgatca gcaggtcgtc cttgagcgcg tcggcggcga ccttcagccg gcgcgcgtaa tcgagcgcct ggtcgtgatc gtggatcgag cacgggccga cgacgagcag cagccggtcg tcgcggccgt gcaggatgtc gccgatcgcg cggcgcgtat cctcgacgag cgtctgcgtg gcggccggca ccggcagctc gtcgagcagc agcgcgggcg aaatcagcgg acgcacggcg ccgatgcgga cgtcgtcgat gcgggtggtg tcctgcgtcg cgtcggcgct gcctacctcg cgatcgtggg aaggattgtc gaggt
Expected Protein Sequence
MAHHHHHHMQ NLDNPSHDRE VGSADATQDT TRIDDVRIGA VRPLISPALL LDELPVPAAT QTLVEDTRRA IGDILHGRDD RLLLVVGPCS IHDHDQALDY ARRLKVAADA LKDDLLITMR VYFEKPRTTV GWKGYINDPR LDGSFRINEG LRAARQLLLD INALGLPAST EFLDLLSPQY IADLIAWGAI GARTTESQSH RQLASGLSCP IGFKNGTDGG VQVAADAIVA AAASHAFMGM TKMGMAAIFE TRGNDDAHVI LRGGKTGPNY DAEHVEATCA VLRKSGLREQ VMVDCSHANS NKSHDRQVDV AQDLARQLSQ GEHRIVGVMV ESHLEAGRQD LKPGVPLQYG VSITDACLSW TQTEPVLDVL AEAVRHRRVY SRNA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga acctcgacaa tccttcccac gatcgcgagg taggcagcgc cgacgcgacg caggacacca cccgcatcga cgacgtccgc atcggcgccg tgcgtccgct gatttcgccc gcgctgctgc tcgacgagct gccggtgccg gccgccacgc agacgctcgt cgaggatacg cgccgcgcga tcggcgacat cctgcacggc cgcgacgacc ggctgctgct cgtcgtcggc ccgtgctcga tccacgatca cgaccaggcg ctcgattacg cgcgccggct gaaggtcgcc gccgacgcgc tcaaggacga cctgctgatc acgatgcgcg tgtacttcga gaagccgcgt acgacggtcg gctggaaggg gtacatcaac gatccgcgcc tggatggcag cttccgcatc aacgaagggc tgcgcgccgc gcggcaactg ctgctcgaca tcaacgcgct cggcctgccc gcatcgaccg aattcctcga cctgctgagc ccgcagtaca tcgccgacct gatcgcgtgg ggcgcgatcg gcgcgcgcac caccgagagc cagagccatc gccagctcgc gtcggggctg agctgcccga tcggcttcaa gaacggcacc gacggcggcg tgcaggtcgc ggcggacgcg atcgttgctg cggcggcgag ccatgcgttc atggggatga cgaagatggg gatggccgcg atcttcgaga cgcgcggcaa cgacgacgcg cacgtgatcc tgcgcggcgg caagaccggc ccgaactacg acgcggagca tgtcgaggcg acctgcgcgg tgctgcgcaa gagcggattg cgcgagcagg tgatggtcga ctgctcgcat gcgaattcca acaagtcgca cgaccggcag gtcgacgtcg cgcaggatct cgcacggcaa ctgtcgcagg gcgagcaccg gatcgtcggc gtcatggtcg agagccacct cgaggccggg cggcaggatc tgaagccggg cgtgccgctg cagtacggcg tgtcgatcac cgatgcatgc ctgagctgga cgcagacgga gccggtgctc gacgtgctgg cggaggcggt gcggcatcgg cgggtgtatt cgcggaatgc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat