BuceA.00090.a

Putative phospho-2-dehydro-3-deoxyheptonate aldolase

CENTER ID: BuceA.00090.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00090.a.B1.GE36719 Full length( BuceA.00090.a ) 1 357
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00090.a.B1.PS01964 Full length( BuceA.00090.a ) 1 357

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3405
RefSeq: YP_002232386.1
UniProt: B4EE04

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPHNTDDVR IRELKELTPP AHLIREFACS EAASELIYHS RQSMHRILHG MDDRLIVVIG PCSIHDTKAA IEYAGRLVKE RERFKGELEI VMRVYFEKPR TTVGWKGLIN DPHLDNSFKI NEGLRTAREL LLQINEMGLP AATEYLDMIS PQYIADLISW GAIGARTTES QVHRELASGL SCPVGFKNGT DGNVKIAVDA IKAASQPHHF LSVTKGGHSA IVSTAGNEDC HVILRGGKAP NYDADSVNAA CADIGKAGLA ARLMIDASHA NSSKKHENQI PVCADIGRQV AAGDERIVGV MIESHLVEGR QDLKEGCALT YGQSITDACI AWDDSVKVLE GLAESVKARR VARGSGN
NT Sequence
ATGCCCCCGC ACAACACCGA CGACGTCCGC ATCCGCGAAC TCAAGGAACT GACGCCGCCC GCCCACCTGA TCCGCGAATT CGCGTGCTCC GAAGCCGCGT CCGAGCTGAT CTACCACTCG CGCCAGTCGA TGCACCGCAT CCTGCACGGG ATGGACGACC GGCTGATCGT CGTGATCGGG CCGTGCTCGA TCCATGACAC GAAGGCGGCG ATCGAATACG CGGGCCGGCT CGTGAAGGAA CGCGAGCGCT TCAAGGGCGA ACTCGAAATC GTGATGCGCG TGTACTTCGA GAAGCCGCGC ACGACGGTCG GCTGGAAGGG GCTCATCAAC GATCCGCACC TCGACAATAG CTTCAAGATC AACGAAGGGC TGCGCACCGC GCGCGAGCTG CTGCTGCAGA TCAACGAAAT GGGGCTGCCG GCAGCAACCG AATACCTCGA CATGATCAGC CCGCAGTACA TCGCCGACCT GATCTCGTGG GGCGCGATCG GCGCGCGCAC GACCGAGTCG CAGGTGCACC GCGAACTCGC GTCGGGGCTG TCGTGCCCGG TCGGCTTCAA GAACGGCACC GACGGCAACG TGAAGATCGC GGTCGACGCG ATCAAGGCCG CGTCGCAGCC GCACCATTTC CTGTCGGTGA CGAAGGGCGG CCATTCGGCG ATCGTGTCGA CGGCCGGCAA CGAGGACTGC CACGTGATCC TGCGCGGCGG CAAGGCGCCG AACTACGACG CGGACAGCGT GAATGCCGCG TGCGCGGACA TCGGCAAGGC CGGCCTCGCC GCGCGTCTGA TGATCGACGC GAGCCATGCG AACAGCTCGA AGAAGCACGA GAACCAGATT CCCGTGTGCG CCGACATCGG CCGCCAGGTC GCGGCCGGCG ACGAACGCAT CGTCGGCGTG ATGATCGAGT CGCACCTGGT CGAGGGCCGT CAGGACCTGA AGGAAGGCTG CGCGCTGACC TACGGCCAGA GCATCACCGA CGCGTGCATC GCGTGGGACG ACAGCGTGAA GGTGCTCGAA GGCCTCGCGG AATCGGTGAA GGCGCGCCGC GTCGCGCGCG GCAGCGGCAA T
Details for BuceA.00090.a.B1.GE36719
HARVESTED ON: 2/27/2013
SEQUENCED ON: 2/2/2013
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 83
Validated AA Sequence
MHRILHGMDD RLIVVIGPCS IHDTKAAIEY AGRLVKERER FKGELEIVMR VYFEKPRTTV GWKGLINDPH LDNSFKINEG LRTARELLLQ INEMGLPAAT EYLDMISPQY IADLISWGAI GARTTESQVH RELASGLSCP VGFKNGTDGN VKIAVDAIKA ASQPHHFLSV TKGGHSAIVS TAGNEDCHVX LRGGKAPNYD ADSVNAACAD IGKAGLAARL MXDASHANSS KKHENQIPVX XDXXRQVAAG DXRXXGXXXE SXXXXXRQXX XXXXXXXXXX SIXXXXXXXX XXVXXXXGLX XXXX
Validated NT Sequence
cctnnnnnnn nnnnnnnagg cctnnnnnnn nnttcacnnt gnnnnnnnnn nnnnnnnnnn nnnnnnngat gctntggnnn nnnntnancn nnnnnncntn nntnnngnnc tgacggcnnt nnnnnnngng ngactcgnnn nnnncnccna nnatgcgttn gtcgccggcc gcgacctggc ggcngangtc ggnncncacg ggaatctggt tctcgtgctt cttcgagctg ttcgcatggc tcgcgtcnat catcagacgc gcggcgaggc cggccttgcc gatgtccgcg cacgcggcat tcacgctgtc cgcgtcgtag ttcggcgcct tgccgccgcg caggannacg tggcagtcct cgttgccggc cgtcgacacg atcgccgaat ggccgccctt cgtcaccgac aggaaatggt gcggctgcga cgcggccttg atcgcgtcga ccgcgatctt cacgttgccg tcggtgccgt tcttgaagcc gaccgggcac gacagccccg acgcgagttc gcggtgcacc tgcgactcgg tcgtgcgcgc gccgatcgcg ccccacgaga tcaggtcggc gatgtactgc gggctgatca tgtcgaggta ttcggttgct gccggcagcc ccatttcgtt gatctgcagc agcagctcgc gcgcggtgcg cagcccttcg ttgatcttga agctattgtc gaggtgcgga tcgttgatga gccccttcca gccgaccgtc gtgcgcggct tctcgaagta cacgcgcatc acgatttcga gttcgccctt gaagcgctcg cgttccttca cgagccggcc cgcgtattcg atcgccgcct tcgtgtcatg gatcgagcac ggcccgatca cgacgatcag ccggtcgtcc atcccgtgca ggatgcggtg catcgactgg cgcgagtggt agatcagctc ggacgcggct tcggagcacg cgaattcgcg gatcaggtgg gcgggcggcg tcagttcctt gagttcgcgg atgcggacgt cgtcggtgtt gtgc
Expected Protein Sequence
MAHHHHHHMP PHNTDDVRIR ELKELTPPAH LIREFACSEA ASELIYHSRQ SMHRILHGMD DRLIVVIGPC SIHDTKAAIE YAGRLVKERE RFKGELEIVM RVYFEKPRTT VGWKGLINDP HLDNSFKINE GLRTARELLL QINEMGLPAA TEYLDMISPQ YIADLISWGA IGARTTESQV HRELASGLSC PVGFKNGTDG NVKIAVDAIK AASQPHHFLS VTKGGHSAIV STAGNEDCHV ILRGGKAPNY DADSVNAACA DIGKAGLAAR LMIDASHANS SKKHENQIPV CADIGRQVAA GDERIVGVMI ESHLVEGRQD LKEGCALTYG QSITDACIAW DDSVKVLEGL AESVKARRVA RGSGN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccc cgcacaacac cgacgacgtc cgcatccgcg aactcaagga actgacgccg cccgcccacc tgatccgcga attcgcgtgc tccgaagccg cgtccgagct gatctaccac tcgcgccagt cgatgcaccg catcctgcac gggatggacg accggctgat cgtcgtgatc gggccgtgct cgatccatga cacgaaggcg gcgatcgaat acgcgggccg gctcgtgaag gaacgcgagc gcttcaaggg cgaactcgaa atcgtgatgc gcgtgtactt cgagaagccg cgcacgacgg tcggctggaa ggggctcatc aacgatccgc acctcgacaa tagcttcaag atcaacgaag ggctgcgcac cgcgcgcgag ctgctgctgc agatcaacga aatggggctg ccggcagcaa ccgaatacct cgacatgatc agcccgcagt acatcgccga cctgatctcg tggggcgcga tcggcgcgcg cacgaccgag tcgcaggtgc accgcgaact cgcgtcgggg ctgtcgtgcc cggtcggctt caagaacggc accgacggca acgtgaagat cgcggtcgac gcgatcaagg ccgcgtcgca gccgcaccat ttcctgtcgg tgacgaaggg cggccattcg gcgatcgtgt cgacggccgg caacgaggac tgccacgtga tcctgcgcgg cggcaaggcg ccgaactacg acgcggacag cgtgaatgcc gcgtgcgcgg acatcggcaa ggccggcctc gccgcgcgtc tgatgatcga cgcgagccat gcgaacagct cgaagaagca cgagaaccag attcccgtgt gcgccgacat cggccgccag gtcgcggccg gcgacgaacg catcgtcggc gtgatgatcg agtcgcacct ggtcgagggc cgtcaggacc tgaaggaagg ctgcgcgctg acctacggcc agagcatcac cgacgcgtgc atcgcgtggg acgacagcgt gaaggtgctc gaaggcctcg cggaatcggt gaaggcgcgc cgcgtcgcgc gcggcagcgg caattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuceA.00090.a.B1.PS01964
PURIFICATION DATe: 1/15/2014
CONCENTRATION: 35mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 110µl
PERCENT IDENTITY: 84
PERCENT COVERAGE: 83
Protocol Notes
notes unavailable
Validated AA Sequence
MHRILHGMDD RLIVVIGPCS IHDTKAAIEY AGRLVKERER FKGELEIVMR VYFEKPRTTV GWKGLINDPH LDNSFKINEG LRTARELLLQ INEMGLPAAT EYLDMISPQY IADLISWGAI GARTTESQVH RELASGLSCP VGFKNGTDGN VKIAVDAIKA ASQPHHFLSV TKGGHSAIVS TAGNEDCHVX LRGGKAPNYD ADSVNAACAD IGKAGLAARL MXDASHANSS KKHENQIPVX XDXXRQVAAG DXRXXGXXXE SXXXXXRQXX XXXXXXXXXX SIXXXXXXXX XXVXXXXGLX XXXX
Validated NT Sequence
cctnnnnnnn nnnnnnnagg cctnnnnnnn nnttcacnnt gnnnnnnnnn nnnnnnnnnn nnnnnnngat gctntggnnn nnnntnancn nnnnnncntn nntnnngnnc tgacggcnnt nnnnnnngng ngactcgnnn nnnncnccna nnatgcgttn gtcgccggcc gcgacctggc ggcngangtc ggnncncacg ggaatctggt tctcgtgctt cttcgagctg ttcgcatggc tcgcgtcnat catcagacgc gcggcgaggc cggccttgcc gatgtccgcg cacgcggcat tcacgctgtc cgcgtcgtag ttcggcgcct tgccgccgcg caggannacg tggcagtcct cgttgccggc cgtcgacacg atcgccgaat ggccgccctt cgtcaccgac aggaaatggt gcggctgcga cgcggccttg atcgcgtcga ccgcgatctt cacgttgccg tcggtgccgt tcttgaagcc gaccgggcac gacagccccg acgcgagttc gcggtgcacc tgcgactcgg tcgtgcgcgc gccgatcgcg ccccacgaga tcaggtcggc gatgtactgc gggctgatca tgtcgaggta ttcggttgct gccggcagcc ccatttcgtt gatctgcagc agcagctcgc gcgcggtgcg cagcccttcg ttgatcttga agctattgtc gaggtgcgga tcgttgatga gccccttcca gccgaccgtc gtgcgcggct tctcgaagta cacgcgcatc acgatttcga gttcgccctt gaagcgctcg cgttccttca cgagccggcc cgcgtattcg atcgccgcct tcgtgtcatg gatcgagcac ggcccgatca cgacgatcag ccggtcgtcc atcccgtgca ggatgcggtg catcgactgg cgcgagtggt agatcagctc ggacgcggct tcggagcacg cgaattcgcg gatcaggtgg gcgggcggcg tcagttcctt gagttcgcgg atgcggacgt cgtcggtgtt gtgc
Expressed Protein Sequence
MAHHHHHHMP PHNTDDVRIR ELKELTPPAH LIREFACSEA ASELIYHSRQ SMHRILHGMD DRLIVVIGPC SIHDTKAAIE YAGRLVKERE RFKGELEIVM RVYFEKPRTT VGWKGLINDP HLDNSFKINE GLRTARELLL QINEMGLPAA TEYLDMISPQ YIADLISWGA IGARTTESQV HRELASGLSC PVGFKNGTDG NVKIAVDAIK AASQPHHFLS VTKGGHSAIV STAGNEDCHV ILRGGKAPNY DADSVNAACA DIGKAGLAAR LMIDASHANS SKKHENQIPV CADIGRQVAA GDERIVGVMI ESHLVEGRQD LKEGCALTYG QSITDACIAW DDSVKVLEGL AESVKARRVA RGSGN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCC CGCACAACAC CGACGACGTC CGCATCCGCG AACTCAAGGA ACTGACGCCG CCCGCCCACC TGATCCGCGA ATTCGCGTGC TCCGAAGCCG CGTCCGAGCT GATCTACCAC TCGCGCCAGT CGATGCACCG CATCCTGCAC GGGATGGACG ACCGGCTGAT CGTCGTGATC GGGCCGTGCT CGATCCATGA CACGAAGGCG GCGATCGAAT ACGCGGGCCG GCTCGTGAAG GAACGCGAGC GCTTCAAGGG CGAACTCGAA ATCGTGATGC GCGTGTACTT CGAGAAGCCG CGCACGACGG TCGGCTGGAA GGGGCTCATC AACGATCCGC ACCTCGACAA TAGCTTCAAG ATCAACGAAG GGCTGCGCAC CGCGCGCGAG CTGCTGCTGC AGATCAACGA AATGGGGCTG CCGGCAGCAA CCGAATACCT CGACATGATC AGCCCGCAGT ACATCGCCGA CCTGATCTCG TGGGGCGCGA TCGGCGCGCG CACGACCGAG TCGCAGGTGC ACCGCGAACT CGCGTCGGGG CTGTCGTGCC CGGTCGGCTT CAAGAACGGC ACCGACGGCA ACGTGAAGAT CGCGGTCGAC GCGATCAAGG CCGCGTCGCA GCCGCACCAT TTCCTGTCGG TGACGAAGGG CGGCCATTCG GCGATCGTGT CGACGGCCGG CAACGAGGAC TGCCACGTGA TCCTGCGCGG CGGCAAGGCG CCGAACTACG ACGCGGACAG CGTGAATGCC GCGTGCGCGG ACATCGGCAA GGCCGGCCTC GCCGCGCGTC TGATGATCGA CGCGAGCCAT GCGAACAGCT CGAAGAAGCA CGAGAACCAG ATTCCCGTGT GCGCCGACAT CGGCCGCCAG GTCGCGGCCG GCGACGAACG CATCGTCGGC GTGATGATCG AGTCGCACCT GGTCGAGGGC CGTCAGGACC TGAAGGAAGG CTGCGCGCTG ACCTACGGCC AGAGCATCAC CGACGCGTGC ATCGCGTGGG ACGACAGCGT GAAGGTGCTC GAAGGCCTCG CGGAATCGGT GAAGGCGCGC CGCGTCGCGC GCGGCAGCGG CAATTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT