SSGCID: Target Page

BuceA.00086.a

3-dehydroquinate dehydratase

CENTER ID:	BuceA.00086.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00086.a.A1.GE33192	Full length( BuceA.00086.a )		1	180

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.6311
RefSeq:	YP_002235105.1
UniProt:	B4EJ55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHGRTLAPNS DVFGAAPILV CPCFRSRNTM SKHKVLVLNG PNLNLLGTRE PHIYGAETLA DLEQRCRTAA EGLGLEIEFR QSNAEHQLID WLHAARHDTH GIVINPAAYT HTSVALADAL SAIAKPVIEV HISNVHRREA FRHHSYISAI AEAVICGCGT EGYVFALQRL ATLFAQGAAR

NT Sequence

GTGCACGGGC GCACACTAGC GCCAAATTCC GATGTCTTCG GGGCGGCGCC GATCCTGGTC TGCCCCTGTT TCAGGAGCAG AAACACGATG AGCAAGCACA AGGTGCTGGT GCTGAACGGC CCGAACCTCA ATCTGCTGGG CACCCGCGAG CCCCATATCT ACGGCGCGGA AACGCTCGCC GATCTCGAGC AGCGCTGCCG CACGGCGGCG GAAGGGCTCG GGCTGGAAAT CGAGTTTCGC CAGTCGAACG CCGAGCACCA GCTGATCGAC TGGCTGCATG CCGCGCGTCA CGACACGCAC GGCATCGTGA TCAACCCGGC CGCCTATACG CACACGTCGG TCGCGCTGGC CGACGCGCTT TCCGCGATCG CGAAGCCGGT GATCGAGGTG CATATCTCGA ACGTCCATCG CCGCGAGGCG TTTCGCCACC ATTCGTATAT CTCGGCGATC GCCGAAGCCG TGATCTGCGG CTGCGGCACC GAAGGCTACG TGTTCGCGCT GCAGCGCCTG GCGACGCTGT TCGCGCAGGG GGCTGCCCGA

Details for BuceA.00086.a.A1.GE33192

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMVHGRTL APNSDVFGAA PILVCPCFRS RNTMSKHKVL VLNGPNLNLL GTREPHIYGA ETLADLEQRC RTAAEGLGLE IEFRQSNAEH QLIDWLHAAR HDTHGIVINP AAYTHTSVAL ADALSAIAKP VIEVHISNVH RREAFRHHSY ISAIAEAVIC GCGTEGYVFA LQRLATLFAQ GAAR

Validated NT Sequence

gcanccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg tgcacgggcg cacactagcg ccaaattccg atgtcttcgg ggcggcgccg atcctggtct gcccctgttt caggagcaga aacacgatga gcaagcacaa ggtgctggtg ctgaacggcc cgaacctcaa tctgctgggc acccgcgagc cccatatcta cggcgcggaa acgctcgccg atctcgagca gcgctgccgc acggcggcgg aagggctcgg gctggaaatc gagtttcgcc agtcgaacgc cgagcaccag ctgatcgact ggctgcatgc cgcgcgtcac gacacgcacg gcatcgtgat caacccggcc gcctatacgc acacgtcggt cgcgctggcc gacgcgcttt ccgcgatcgc gaagccggtg atcgaggtgc atatctcgaa cgtccatcgc cgcgaggcgt ttcgccacca ttcgtatatc tcggcgatcg ccgaagccgt gatctgcggc tgcggcaccg aaggctacgt gttcgcgctg cagcgcctgg cgacgctgtt cgcgcagggg gctgcccgat aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca nnnnnnnnna nnnnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVHGRTLAP NSDVFGAAPI LVCPCFRSRN TMSKHKVLVL NGPNLNLLGT REPHIYGAET LADLEQRCRT AAEGLGLEIE FRQSNAEHQL IDWLHAARHD THGIVINPAA YTHTSVALAD ALSAIAKPVI EVHISNVHRR EAFRHHSYIS AIAEAVICGC GTEGYVFALQ RLATLFAQGA AR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcacggg cgcacactag cgccaaattc cgatgtcttc ggggcggcgc cgatcctggt ctgcccctgt ttcaggagca gaaacacgat gagcaagcac aaggtgctgg tgctgaacgg cccgaacctc aatctgctgg gcacccgcga gccccatatc tacggcgcgg aaacgctcgc cgatctcgag cagcgctgcc gcacggcggc ggaagggctc gggctggaaa tcgagtttcg ccagtcgaac gccgagcacc agctgatcga ctggctgcat gccgcgcgtc acgacacgca cggcatcgtg atcaacccgg ccgcctatac gcacacgtcg gtcgcgctgg ccgacgcgct ttccgcgatc gcgaagccgg tgatcgaggt gcatatctcg aacgtccatc gccgcgaggc gtttcgccac cattcgtata tctcggcgat cgccgaagcc gtgatctgcg gctgcggcac cgaaggctac gtgttcgcgc tgcagcgcct ggcgacgctg ttcgcgcagg gggctgcccg ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa