BuceA.00085.e

Putative UDP-glucose 4-epimerase

CENTER ID: BuceA.00085.e
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00085.e.B1.GE36718 Full length( BuceA.00085.e ) 1 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.5800
RefSeq: YP_002234638.1
UniProt: B4EN76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVLVTGGAG YIGSHTCKAL AEAGHEPVAY DNLSTGHRDA VRWGPLVAAD ILDRDALSRA LAAHRPDVVI HFAALAYVGE SVLAPERYYT VNVTGTCTLL SAMRAAGVGR IVMSSSCATY GIPEALPISE RTPQRPINPY GFTKYAMERM AADFERAYGL KWIALRYFNA AGADPDGAIG ECHAPETHAL PLAIRAALRT GDAFRVMGTD YPTPDGSAIR DYVHVSDLAD AHLKASAYLC GGGPSVALNL GTGNGTSVLA ALRAVEAVTG RRVPTVLAAR RPGDPPALYA DATMAARVLG WRPRFTAIEP MVEHAAAWFQ KERQRESSR
NT Sequence
ATGAAAGTGC TCGTGACAGG GGGAGCGGGC TATATCGGCA GCCATACGTG CAAGGCGCTC GCGGAAGCGG GACACGAGCC CGTCGCGTAC GACAACCTGT CGACCGGTCA TCGCGACGCG GTCCGCTGGG GGCCGCTCGT CGCGGCCGAC ATCCTCGACC GCGATGCGCT GTCGCGCGCG CTCGCCGCGC ACCGGCCCGA CGTCGTGATC CATTTCGCCG CGCTCGCCTA CGTCGGCGAA TCGGTGCTCG CGCCCGAGCG CTACTACACG GTCAACGTGA CGGGCACCTG CACGCTGCTG AGCGCGATGC GCGCAGCCGG CGTGGGCAGG ATCGTGATGT CGTCGTCGTG CGCGACGTAC GGCATTCCGG AGGCGCTGCC GATCTCGGAG CGCACGCCGC AGCGGCCGAT CAATCCGTAC GGCTTCACGA AGTACGCGAT GGAGCGGATG GCCGCCGATT TCGAACGCGC GTACGGGCTG AAATGGATCG CGCTGCGCTA CTTCAACGCG GCGGGCGCGG ACCCCGACGG CGCGATCGGC GAATGCCACG CACCGGAAAC GCACGCGCTG CCGCTGGCGA TCCGCGCGGC GCTGCGCACC GGCGACGCAT TCCGGGTAAT GGGCACCGAC TACCCGACGC CGGACGGCTC GGCGATTCGC GACTACGTGC ACGTGAGCGA CCTTGCCGAC GCCCACCTGA AGGCAAGCGC CTATCTGTGC GGCGGCGGCC CGAGCGTCGC GCTGAACCTC GGCACCGGAA ACGGGACGTC GGTGCTCGCC GCGTTGCGCG CGGTCGAGGC CGTGACGGGC CGCCGCGTGC CGACGGTGCT GGCCGCGCGC CGCCCGGGCG ATCCGCCCGC GCTGTACGCC GACGCGACGA TGGCCGCGCG GGTGCTCGGC TGGCGGCCCC GCTTCACCGC GATCGAGCCG ATGGTCGAAC ACGCTGCGGC GTGGTTTCAG AAGGAGCGGC AACGCGAATC CAGCAGG
Details for BuceA.00085.e.B1.GE36718
HARVESTED ON: 2/27/2013
SEQUENCED ON: 3/1/2013
EXPECTED MW: 37kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 58
Validated AA Sequence
MAHHHHHHMK VLVTGGAGYI GSHTCKALAE AGHEPVAXXX LSTGHRDAVR WGPLVAADIL DRDALSRALA AHRPDVVIHF AALAYVGESV LAPERYYTVN VTGTCTLLSA MRAAGVGRIV MSSSCATYGI PEALPISERT PQRPINPYGF TKYAMERMAA DFERAYGLKW IALRYFNAAG ADPDGAIGEC HAPETHALPL AI
Validated NT Sequence
ataccatggc tcaccaccac caccaccata tgaaagtgct cgtgacaggg ggagcgggct atatcggcag ccatacgtgc aaggcgctcg cggaagcggg acacgagccc gtcgcgtann annncctgtc gaccggtcat cgcgacgcgg tccgctgggg gccgctcgtc gcggccgaca tcctcgaccg cgatgcgctg tcgcgcgcgc tcgccgcgca ccggcccgac gtcgtgatcc atttcgccgc gctcgcctac gtcggcgaat cggtgctcgc gcccgagcgc tactacacgg tcaacgtgac gggcacctgc acgctgctga gcgcgatgcg cgcagccggc gtgggcagga tcgtgatgtc gtcgtcgtgc gcgacgtacg gcattccgga ggcgctgccg atctcggagc gcacgccgca gcggccgatc aatccgtacg gcttcacgaa gtacgcgatg gagcggatgg ccgccgattt cgaacgcgcg tacgggctga aatggatcgc gctgcgctac ttcaacgcgg cgggcgcgga ccccgacggc gcgatcggcg aatgccacgc accggaaacg cacgcgctgc cgctggcgat ccg
Expected Protein Sequence
MAHHHHHHMK VLVTGGAGYI GSHTCKALAE AGHEPVAYDN LSTGHRDAVR WGPLVAADIL DRDALSRALA AHRPDVVIHF AALAYVGESV LAPERYYTVN VTGTCTLLSA MRAAGVGRIV MSSSCATYGI PEALPISERT PQRPINPYGF TKYAMERMAA DFERAYGLKW IALRYFNAAG ADPDGAIGEC HAPETHALPL AIRAALRTGD AFRVMGTDYP TPDGSAIRDY VHVSDLADAH LKASAYLCGG GPSVALNLGT GNGTSVLAAL RAVEAVTGRR VPTVLAARRP GDPPALYADA TMAARVLGWR PRFTAIEPMV EHAAAWFQKE RQRESSR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag tgctcgtgac agggggagcg ggctatatcg gcagccatac gtgcaaggcg ctcgcggaag cgggacacga gcccgtcgcg tacgacaacc tgtcgaccgg tcatcgcgac gcggtccgct gggggccgct cgtcgcggcc gacatcctcg accgcgatgc gctgtcgcgc gcgctcgccg cgcaccggcc cgacgtcgtg atccatttcg ccgcgctcgc ctacgtcggc gaatcggtgc tcgcgcccga gcgctactac acggtcaacg tgacgggcac ctgcacgctg ctgagcgcga tgcgcgcagc cggcgtgggc aggatcgtga tgtcgtcgtc gtgcgcgacg tacggcattc cggaggcgct gccgatctcg gagcgcacgc cgcagcggcc gatcaatccg tacggcttca cgaagtacgc gatggagcgg atggccgccg atttcgaacg cgcgtacggg ctgaaatgga tcgcgctgcg ctacttcaac gcggcgggcg cggaccccga cggcgcgatc ggcgaatgcc acgcaccgga aacgcacgcg ctgccgctgg cgatccgcgc ggcgctgcgc accggcgacg cattccgggt aatgggcacc gactacccga cgccggacgg ctcggcgatt cgcgactacg tgcacgtgag cgaccttgcc gacgcccacc tgaaggcaag cgcctatctg tgcggcggcg gcccgagcgt cgcgctgaac ctcggcaccg gaaacgggac gtcggtgctc gccgcgttgc gcgcggtcga ggccgtgacg ggccgccgcg tgccgacggt gctggccgcg cgccgcccgg gcgatccgcc cgcgctgtac gccgacgcga cgatggccgc gcgggtgctc ggctggcggc cccgcttcac cgcgatcgag ccgatggtcg aacacgctgc ggcgtggttt cagaaggagc ggcaacgcga atccagcagg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat