BuceA.00085.d

Putative UDP-glucuronic acid decarboxylase

CENTER ID: BuceA.00085.d
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00085.d.B1.GE36653 Full length( BuceA.00085.d ) 1 335
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.5799
RefSeq: YP_002234637.1
UniProt: B4EN75

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNDRVAIDTR AGRRVLVTGG AGFLGSYVCE RLVMEGAFVT CVDSLLTGRK LNVADLKASG RFEFVKGDVS LGLPQLQVDE IWNLACAASP PTYQIDPVHT MMTNVLGMNH CLALARRTGA RVFQASTSEI YGDPGVHPQM ETYRGNVNTI GPRACYDEGK RAAEALCYDY YRSHGVDVRV ARIFNTYGPR MSPRDGRVVS NFIVAALNGA ALEIYGDGRQ TRSFCFVSDL IDGFFSLMDA PRNVGTPVNI GNPGEFTMIE LAEQVLAITG STSEIVFRPL PIDDPHQRKP DISVAATELG WRPAIDLDEG LRRTVDYFSR ELWLAPVLRG TGAVA
NT Sequence
ATGAATGACA GGGTCGCGAT CGACACGCGC GCCGGCCGGC GCGTACTGGT GACGGGTGGC GCAGGTTTTC TCGGCAGCTA CGTCTGCGAA CGGCTCGTCA TGGAAGGCGC GTTCGTCACC TGCGTCGACA GCCTGCTGAC GGGACGCAAG CTCAACGTCG CCGACCTGAA GGCGTCCGGC CGGTTCGAAT TCGTGAAGGG CGACGTGTCG CTCGGCCTGC CGCAGCTGCA GGTGGATGAA ATCTGGAATC TCGCGTGCGC GGCCTCCCCG CCCACCTACC AGATCGATCC GGTCCACACG ATGATGACGA ACGTGCTCGG CATGAACCAC TGTCTCGCGC TGGCGCGCCG GACCGGCGCG CGCGTGTTCC AGGCGTCGAC CAGCGAGATC TACGGCGACC CCGGCGTCCA TCCGCAGATG GAAACCTATC GCGGCAACGT GAACACGATC GGCCCGCGTG CGTGCTACGA CGAAGGCAAG CGTGCGGCCG AGGCGCTGTG CTACGACTAC TACCGTTCGC ACGGCGTCGA CGTGCGCGTC GCGCGCATCT TCAATACCTA CGGGCCGCGC ATGTCGCCGC GCGACGGCCG CGTCGTGTCG AACTTCATCG TCGCCGCGCT CAATGGCGCC GCGCTCGAGA TCTACGGCGA CGGCCGGCAG ACACGCTCGT TCTGCTTCGT CAGCGACCTG ATCGACGGCT TCTTCAGCCT GATGGACGCC CCGCGCAACG TCGGCACACC GGTCAACATC GGCAATCCCG GCGAATTCAC GATGATCGAA CTTGCGGAGC AAGTCCTCGC GATCACGGGC TCGACCTCGG AGATCGTGTT CCGGCCGCTG CCGATCGACG ATCCGCACCA GCGCAAGCCC GACATCTCGG TCGCCGCGAC GGAGCTCGGC TGGCGGCCGG CGATCGATCT CGACGAAGGC CTGCGCCGCA CGGTCGACTA TTTCAGCCGC GAACTGTGGC TCGCGCCGGT GCTGCGCGGG ACCGGAGCGG TCGCA
Details for BuceA.00085.d.B1.GE36653
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 89
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMN DRVAIDTRAG RRVLVTGGAG FLGSYVCERL VMEGAFVTCV DSLLTGRKLN VADLKASGRF EFVKGDVSLG LPQLQVDEIW NLACAASPPT YQIDPVHTMM TNVLGMNHCL ALARRTGARV FQASTSEIYG DPGVHPQMET YRGNVNTIGP RACYDEGKRA AEALCYDYYR SHGVDVRVAR IFNTYGPRMS PRDGRVVSNF IVAALNGAAL EIYGDGRQTR SFCFVSDLID GFFSLMDAPR NVGTPVNIGX PGEFTMIELA EQVLAITGST SEIVFRPLPI DXPXQXXPXX XXTXLXWRXA IDLDXXXRXX IXAXNXXRXX CXGXXXXXX
Validated NT Sequence
anattttgtt nnnnttaaga aggagatata ccatggctca ccaccaccac caccatatga atgacagggt cgcgatcgac acgcgcgccg gccggcgcgt actggtgacg ggtggcgcag gttttctcgg cagctacgtc tgcgaacggc tcgtcatgga aggcgcgttc gtcacctgcg tcgacagcct gctgacggga cgcaagctca acgtcgccga cctgaaggcg tccggccggt tcgaattcgt gaagggcgac gtgtcgctcg gcctgccgca gctgcaggtg gatgaaatct ggaatctcgc gtgcgcggcc tccccgccca cctaccagat cgatccggtc cacacgatga tgacgaacgt gctcggcatg aaccactgtc tcgcgctggc gcgccggacc ggcgcgcgcg tgttccaggc gtcgaccagc gagatctacg gcgaccccgg cgtccatccg cagatggaaa cctatcgcgg caacgtgaac acgatcggcc cgcgtgcgtg ctacgacgaa ggcaagcgtg cggccgaggc gctgtgctac gactactacc gttcgcacgg cgtcgacgtg cgcgtcgcgc gcatcttcaa tacctacggg ccgcgcatgt cgccgcgcga cggccgcgtc gtgtcgaact tcatcgtcgc cgcgctcaat ggcgccgcgc tcgagatcta cggcgacggc cggcagacac gctcgttctg cttcgtcagc gacctgatcg acggcttctt cagcctgatg gacgccccgc gcaacgtcgg cacaccggtc aacatcggca ntcccggcga attcacgatg atcgaacttg cggagcaagt cctcgcgatc acgggctcga cctcngagat cgtgttccgg ccgctgccga tcgacnatcc gcancagcnn nagcccnann tcnccncnac gganctcnnc tggcggncgg cgatcgatct cgacntgcnc ncacggncga nnatttnagc cncnaactgn gntcgcncgn nctgcncngg nncggannnn ncncntnant gagtaanann natcnnnnnn nnnnnnnnnn nnnnaa
Expected Protein Sequence
MAHHHHHHMN DRVAIDTRAG RRVLVTGGAG FLGSYVCERL VMEGAFVTCV DSLLTGRKLN VADLKASGRF EFVKGDVSLG LPQLQVDEIW NLACAASPPT YQIDPVHTMM TNVLGMNHCL ALARRTGARV FQASTSEIYG DPGVHPQMET YRGNVNTIGP RACYDEGKRA AEALCYDYYR SHGVDVRVAR IFNTYGPRMS PRDGRVVSNF IVAALNGAAL EIYGDGRQTR SFCFVSDLID GFFSLMDAPR NVGTPVNIGN PGEFTMIELA EQVLAITGST SEIVFRPLPI DDPHQRKPDI SVAATELGWR PAIDLDEGLR RTVDYFSREL WLAPVLRGTG AVA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg acagggtcgc gatcgacacg cgcgccggcc ggcgcgtact ggtgacgggt ggcgcaggtt ttctcggcag ctacgtctgc gaacggctcg tcatggaagg cgcgttcgtc acctgcgtcg acagcctgct gacgggacgc aagctcaacg tcgccgacct gaaggcgtcc ggccggttcg aattcgtgaa gggcgacgtg tcgctcggcc tgccgcagct gcaggtggat gaaatctgga atctcgcgtg cgcggcctcc ccgcccacct accagatcga tccggtccac acgatgatga cgaacgtgct cggcatgaac cactgtctcg cgctggcgcg ccggaccggc gcgcgcgtgt tccaggcgtc gaccagcgag atctacggcg accccggcgt ccatccgcag atggaaacct atcgcggcaa cgtgaacacg atcggcccgc gtgcgtgcta cgacgaaggc aagcgtgcgg ccgaggcgct gtgctacgac tactaccgtt cgcacggcgt cgacgtgcgc gtcgcgcgca tcttcaatac ctacgggccg cgcatgtcgc cgcgcgacgg ccgcgtcgtg tcgaacttca tcgtcgccgc gctcaatggc gccgcgctcg agatctacgg cgacggccgg cagacacgct cgttctgctt cgtcagcgac ctgatcgacg gcttcttcag cctgatggac gccccgcgca acgtcggcac accggtcaac atcggcaatc ccggcgaatt cacgatgatc gaacttgcgg agcaagtcct cgcgatcacg ggctcgacct cggagatcgt gttccggccg ctgccgatcg acgatccgca ccagcgcaag cccgacatct cggtcgccgc gacggagctc ggctggcggc cggcgatcga tctcgacgaa ggcctgcgcc gcacggtcga ctatttcagc cgcgaactgt ggctcgcgcc ggtgctgcgc gggaccggag cggtcgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat