BuceA.00085.c

NAD dependent epimerase/dehydratase family protein

CENTER ID: BuceA.00085.c
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00085.c.B2.GE36652 Mature protein( BuceA.00085.c ) 23 335
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6564
RefSeq: YP_002235335.1
UniProt: B4EL03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDTSRDLVL VTGASGFVGS AVARIAQQKG YAVRVLVRPT SPRTNVADLD AEIVTGDMRD EASMRAALRG VRYLLHVAAD YRLWAPDPDE IERANLEGAV ATMRAARAAG VERIVYTSSV ATLKVTSAGD PSDENRPLTA EQAIGVYKRS KVLAERAVER MIADEGLPAV IVNPSTPIGP RDVKPTPTGR IIVEAALGKI PAFVDTGLNL VHVDDVAHGH FLALERGRIG ERYILGGENL PLQQMLADIA QMTGRKAPTI ALPRWPLYPL AVGAEAVAKF TKKEPFVTVD GLRMSKNKMY FTSAKAEREL GYRARPYREG LRDALDWFGS AGYLK
NT Sequence
ATGACTGATA CCTCCCGCGA TCTCGTTCTC GTCACCGGCG CGTCCGGTTT CGTCGGCTCG GCCGTCGCGC GCATCGCGCA GCAGAAAGGC TATGCGGTGC GCGTGCTGGT GCGCCCGACC AGCCCGCGCA CGAACGTCGC GGATCTCGAC GCCGAGATCG TCACCGGCGA CATGCGCGAC GAGGCGTCGA TGCGCGCCGC GCTGCGCGGC GTGCGCTACC TGCTGCACGT GGCGGCCGAC TACCGGCTGT GGGCGCCCGA CCCCGACGAG ATCGAGCGCG CGAACCTCGA AGGCGCGGTC GCGACGATGC GCGCGGCCCG CGCGGCAGGC GTCGAGCGGA TCGTCTACAC GAGCAGCGTC GCGACGCTGA AGGTGACGAG CGCCGGCGAT CCGTCCGACG AGAACCGGCC GCTCACGGCC GAGCAGGCGA TCGGCGTCTA CAAGCGCAGC AAGGTGCTCG CGGAGCGCGC GGTCGAACGG ATGATCGCCG ACGAAGGGCT GCCGGCCGTG ATCGTCAACC CGTCGACGCC GATCGGCCCG CGCGACGTGA AGCCGACGCC GACCGGCCGC ATCATCGTCG AGGCCGCGCT CGGCAAGATT CCCGCGTTCG TCGATACGGG GCTGAACCTC GTGCACGTCG ACGACGTCGC GCACGGCCAC TTCCTCGCCC TCGAGCGCGG CCGGATCGGC GAGCGCTACA TCCTCGGCGG CGAGAACCTG CCGCTGCAGC AGATGCTCGC GGACATCGCG CAGATGACGG GCCGCAAGGC GCCGACGATC GCGCTGCCGC GCTGGCCGCT GTACCCGCTC GCGGTCGGTG CCGAAGCGGT CGCGAAGTTC ACGAAGAAGG AGCCGTTCGT CACCGTCGAC GGGCTGCGGA TGTCGAAGAA CAAGATGTAT TTCACGTCGG CGAAGGCCGA GCGCGAGCTC GGCTACCGTG CGCGCCCGTA TCGCGAAGGG CTGCGCGATG CGCTGGACTG GTTCGGCTCG GCGGGCTACC TCAAG
Details for BuceA.00085.c.B2.GE36652
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 85
PERCENT COVERAGE: 101
Validated AA Sequence
MAHHHHHHMA RIAQQKGYAV RVLVRPTSPR TNVADLDAEI VTGDMRDEAS MRAALRGVRY LLHVAADYRL WAPDPDEIER ANLEGAVATM RAARAAGVER IVYTSSVATL KVTSAGDPSD ENRPLTAEQA IGVYKRSKVL AERAVERMIA DEGLPAVIVN PSTPIGPRDV KPTPTGRIIV EAALGKIPAF VDTGLNLVHV DDVAHGHFLA LERGRIGERY ILGGENLPLQ QMLADIAQMT GRKAPTIALP RWPLYPLAVG AEAVAKFTKK GAVRHRXRAA DVEEQDVFHV GEXRARAXLP CAPVSRRXAR CXGLXRLXGL XQVSEXXR
Validated NT Sequence
attttgttnn ntttaagaag gagatatacc atggctcacc accaccacca ccatatggcg cgcatcgcgc agcagaaagg ctatgcggtg cgcgtgctgg tgcgcccgac cagcccgcgc acgaacgtcg cggatctcga cgccgagatc gtcaccggcg acatgcgcga cgaggcgtcg atgcgcgccg cgctgcgcgg cgtgcgctac ctgctgcacg tggcggccga ctaccggctg tgggcgcccg accccgacga gatcgagcgc gcgaacctcg aaggcgcggt cgcgacgatg cgcgcggccc gcgcggcagg cgtcgagcgg atcgtctaca cgagcagcgt cgcgacgctg aaggtgacga gcgccggcga tccgtccgac gagaaccggc cgctcacggc cgagcaggcg atcggcgtct acaagcgcag caaggtgctc gcggagcgcg cggtcgaacg gatgatcgcc gacgaagggc tgccggccgt gatcgtcaac ccgtcgacgc cgatcggccc gcgcgacgtg aagccgacgc cgaccggccg catcatcgtc gaggccgcgc tcggcaagat tcccgcgttc gtcgatacgg ggctgaacct cgtgcacgtc gacgacgtcg cgcacggcca cttcctcgcc ctcgagcgcg gccggatcgg cgagcgctac atcctcggcg gcgagaacct gccgctgcag cagatgctcg cggacatcgc gcagatgacg ggccgcaagg cgccgacgat cgcgctgccg cgctggccgc tgtacccgct cgcggtcggt gccgaagcgg tcgcgaagtt cacgaagaaa ggagccgttc gtcaccgtcn acgggctgcg gatgtcgaag aacaagatgt atttcacgtc ggcgaangnc gagcgcgagc tcngctaccg tgcgcgcccg tatcgcgaag ggntgcncga tgcnctggnc tggntcggct cngcgggcta cntcaagtaa gtgagtanna naggatc
Expected Protein Sequence
MAHHHHHHMA RIAQQKGYAV RVLVRPTSPR TNVADLDAEI VTGDMRDEAS MRAALRGVRY LLHVAADYRL WAPDPDEIER ANLEGAVATM RAARAAGVER IVYTSSVATL KVTSAGDPSD ENRPLTAEQA IGVYKRSKVL AERAVERMIA DEGLPAVIVN PSTPIGPRDV KPTPTGRIIV EAALGKIPAF VDTGLNLVHV DDVAHGHFLA LERGRIGERY ILGGENLPLQ QMLADIAQMT GRKAPTIALP RWPLYPLAVG AEAVAKFTKK EPFVTVDGLR MSKNKMYFTS AKAERELGYR ARPYREGLRD ALDWFGSAGY LK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc gcatcgcgca gcagaaaggc tatgcggtgc gcgtgctggt gcgcccgacc agcccgcgca cgaacgtcgc ggatctcgac gccgagatcg tcaccggcga catgcgcgac gaggcgtcga tgcgcgccgc gctgcgcggc gtgcgctacc tgctgcacgt ggcggccgac taccggctgt gggcgcccga ccccgacgag atcgagcgcg cgaacctcga aggcgcggtc gcgacgatgc gcgcggcccg cgcggcaggc gtcgagcgga tcgtctacac gagcagcgtc gcgacgctga aggtgacgag cgccggcgat ccgtccgacg agaaccggcc gctcacggcc gagcaggcga tcggcgtcta caagcgcagc aaggtgctcg cggagcgcgc ggtcgaacgg atgatcgccg acgaagggct gccggccgtg atcgtcaacc cgtcgacgcc gatcggcccg cgcgacgtga agccgacgcc gaccggccgc atcatcgtcg aggccgcgct cggcaagatt cccgcgttcg tcgatacggg gctgaacctc gtgcacgtcg acgacgtcgc gcacggccac ttcctcgccc tcgagcgcgg ccggatcggc gagcgctaca tcctcggcgg cgagaacctg ccgctgcagc agatgctcgc ggacatcgcg cagatgacgg gccgcaaggc gccgacgatc gcgctgccgc gctggccgct gtacccgctc gcggtcggtg ccgaagcggt cgcgaagttc acgaagaagg agccgttcgt caccgtcgac gggctgcgga tgtcgaagaa caagatgtat ttcacgtcgg cgaaggccga gcgcgagctc ggctaccgtg cgcgcccgta tcgcgaaggg ctgcgcgatg cgctggactg gttcggctcg gcgggctacc tcaagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat