BuceA.00085.b

dTDP-glucose 4,6-dehydratase

CENTER ID: BuceA.00085.b
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00085.b.B1.GE36651 Full length( BuceA.00085.b ) 1 353
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3252
RefSeq: YP_002232244.1
UniProt: B4ECS6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MILVTGGAGF IGANYVLDWL RASDEAVLNV DKLTYAGNLR TLQSLDRNPK HVFARVDICD RGALDTLFAE HKPRAVLHFA AESHVDRSIH GPADFVQTNV VGTFTLLEVA RQYWNGLSDN DKAAFRFLHV STDEVFGSLS ATDPQFSETT PYAPNSPYSA TKAGSDHLVR AYHHTYGLPT LTTNCSNNYG PYQFPEKLIP LMIANALAGK PLPVYGDGQN VRDWLYVGDH CSAIREVLAR GVPGETYNIG GWNEKKNLEV VHTLCDLLDR ARPKAAGSYR DQITYVTDRP GHDRRYAIDA RKLERELGWK PAETFETGMA KTVDWYLDNQ VWADEVASGE YRKWVETNYA KRT
NT Sequence
ATGATCCTGG TTACGGGCGG CGCGGGTTTT ATCGGTGCCA ATTATGTACT CGACTGGTTG CGCGCATCCG ACGAAGCCGT GCTCAACGTC GACAAGCTGA CGTACGCCGG GAACCTGCGC ACGTTGCAGT CGCTTGACCG GAACCCGAAA CATGTTTTTG CGCGCGTCGA CATCTGCGAT CGCGGTGCGC TTGACACGCT GTTTGCCGAG CACAAGCCCC GTGCCGTGCT GCACTTCGCT GCGGAAAGCC ACGTCGATCG CTCGATCCAC GGTCCGGCGG ACTTCGTCCA GACCAACGTG GTCGGCACGT TCACGCTGCT GGAGGTCGCC CGTCAATACT GGAACGGCCT GAGCGACAAC GACAAGGCGG CCTTCCGCTT CCTGCATGTG TCCACAGACG AGGTGTTCGG TTCGCTGTCG GCCACCGACC CGCAATTCTC CGAAACGACG CCGTACGCGC CGAACAGCCC ATATTCCGCG ACGAAAGCCG GCTCGGATCA CTTGGTGCGC GCCTACCATC ATACGTATGG CTTGCCGACA CTGACGACGA ACTGTTCGAA CAACTACGGT CCGTACCAGT TCCCCGAAAA GCTAATTCCG CTGATGATCG CGAACGCGCT CGCGGGTAAG CCGCTGCCGG TGTACGGCGA TGGCCAGAAC GTGCGCGACT GGCTGTATGT CGGCGATCAC TGCAGCGCGA TTCGCGAAGT GCTCGCGCGC GGCGTGCCCG GCGAGACATA CAACATCGGC GGCTGGAACG AGAAGAAGAA CCTCGAAGTC GTCCATACGC TCTGCGATCT GCTCGACCGC GCCCGTCCGA AAGCGGCCGG CTCGTATCGC GACCAGATTA CCTACGTGAC GGATCGTCCC GGTCACGACC GTCGTTATGC GATCGATGCG CGCAAGCTCG AACGCGAACT GGGCTGGAAG CCGGCAGAGA CGTTCGAAAC CGGCATGGCG AAGACGGTCG ACTGGTATCT CGACAACCAG GTCTGGGCCG ATGAGGTCGC ATCGGGTGAG TACCGCAAGT GGGTCGAAAC GAACTACGCG AAACGCACC
Details for BuceA.00085.b.B1.GE36651
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 23
PERCENT COVERAGE: 47
Validated AA Sequence
MMVGAHQVIR AGFRRGIWAV RRVRRRFGEL RVGGRQRTEH LVCGHMQEAE GRLVVVAQAV PVLTGDLQQR ERADHVGLDE VRRTVDRAID VAFRSEVQHG TGLVLGKQRV KRTAIADVDA RKNMFRVPVK RLQRAQVPGV RQLVDVEHGF VGCAQPVEYI IGTDKTRAAR N
Validated NT Sequence
cccacttgcg gtactcaccc gatgcgacct catcggccca gacctggttg tcgagatacc agtcgaccgt cttcgccatg ccggtttcga acgtctctgc cggcttccag cccagttcgc gttcgagctt gcgcgcatcg atcgcataac gacggtcgtg accgggacga tccgtcacgt aggtaatctg gtcgcgatac gagccggccg ctttcggacg ggcgcggtcg agcagatcgc agagcgtatg gacgacttcg aggttcttct tctcgttcca gccgccgatg ttgtatgtct cgccgggcac gccgcgcgcg agcacttcgc gaatcgcgct gcagtgatcg ccgacataca gccagtcgcg cacgttctgg ccatcgccgt acaccggcag cggcttaccc gcgagcgcgt tcgcgatcat cagcggaatt agcttttcgg ggaactggta cggaccgtag ttgttcgaac agttcgtcgt cagtgtcggc aagccatacg tatgatggta ggcgcgcacc aagtgatccg agccggcttt cgtcgcggaa tatgggctgt tcggcgcgta cggcgtcgtt tcggagaatt gcgggtcggt ggccgacagc gaaccgaaca cctcgtctgt ggacacatgc aggaagcgga aggccgcctt gtcgttgtcg ctcaggccgt tccagtattg acgggcgacc tccagcagcg tgaacgtgcc gaccacgttg gtctggacga agtccgccgg accgtggatc gagcgatcga cgtggctttc cgcagcgaag tgcagcacgg cacggggctt gtgctcggca aacagcgtgt caagcgcacc gcgatcgcag atgtcgacgc gcgcaaaaac atgtttcggg ttccggtcaa gcgactgcaa cgtgcgcagg ttcccggcgt acgtcagctt gtcgacgttg agcacggctt cgtcggatgc gcgcaaccag tcgagtacat aattggcacc gataaaaccc gcgccgcccg taaccag
Expected Protein Sequence
MAHHHHHHMI LVTGGAGFIG ANYVLDWLRA SDEAVLNVDK LTYAGNLRTL QSLDRNPKHV FARVDICDRG ALDTLFAEHK PRAVLHFAAE SHVDRSIHGP ADFVQTNVVG TFTLLEVARQ YWNGLSDNDK AAFRFLHVST DEVFGSLSAT DPQFSETTPY APNSPYSATK AGSDHLVRAY HHTYGLPTLT TNCSNNYGPY QFPEKLIPLM IANALAGKPL PVYGDGQNVR DWLYVGDHCS AIREVLARGV PGETYNIGGW NEKKNLEVVH TLCDLLDRAR PKAAGSYRDQ ITYVTDRPGH DRRYAIDARK LERELGWKPA ETFETGMAKT VDWYLDNQVW ADEVASGEYR KWVETNYAKR T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatcc tggttacggg cggcgcgggt tttatcggtg ccaattatgt actcgactgg ttgcgcgcat ccgacgaagc cgtgctcaac gtcgacaagc tgacgtacgc cgggaacctg cgcacgttgc agtcgcttga ccggaacccg aaacatgttt ttgcgcgcgt cgacatctgc gatcgcggtg cgcttgacac gctgtttgcc gagcacaagc cccgtgccgt gctgcacttc gctgcggaaa gccacgtcga tcgctcgatc cacggtccgg cggacttcgt ccagaccaac gtggtcggca cgttcacgct gctggaggtc gcccgtcaat actggaacgg cctgagcgac aacgacaagg cggccttccg cttcctgcat gtgtccacag acgaggtgtt cggttcgctg tcggccaccg acccgcaatt ctccgaaacg acgccgtacg cgccgaacag cccatattcc gcgacgaaag ccggctcgga tcacttggtg cgcgcctacc atcatacgta tggcttgccg acactgacga cgaactgttc gaacaactac ggtccgtacc agttccccga aaagctaatt ccgctgatga tcgcgaacgc gctcgcgggt aagccgctgc cggtgtacgg cgatggccag aacgtgcgcg actggctgta tgtcggcgat cactgcagcg cgattcgcga agtgctcgcg cgcggcgtgc ccggcgagac atacaacatc ggcggctgga acgagaagaa gaacctcgaa gtcgtccata cgctctgcga tctgctcgac cgcgcccgtc cgaaagcggc cggctcgtat cgcgaccaga ttacctacgt gacggatcgt cccggtcacg accgtcgtta tgcgatcgat gcgcgcaagc tcgaacgcga actgggctgg aagccggcag agacgttcga aaccggcatg gcgaagacgg tcgactggta tctcgacaac caggtctggg ccgatgaggt cgcatcgggt gagtaccgca agtgggtcga aacgaactac gcgaaacgca cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat