BuceA.00081.b

Cysteine desulfurase

CENTER ID: BuceA.00081.b
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00081.b.A1.GE33191 Full length( BuceA.00081.b ) 1 403
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00081.b.A1.PW36053 Full length( BuceA.00081.b ) 1 403

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4436
RefSeq: YP_002233355.1
UniProt: B4EM34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQSRPIYMDY SATTPVDPRV VAKMVPFLHE QFGNPASRSH AYGWDAEHAV EEARAHVAAL LGADPREIVW TSGATEGNNL AIKGAAHFYQ GKGKHLVTVK TEHKAVLDTC RELERQGFDV TYLDVREDGL LDLDALQQAL RADTILVSVM LANNETGVIQ PVADIGALCR ARGIVFHCDA VQAAGKIPVD VNALNVDLLT VTAHKVYGPK GIGALYVRRK PRVRLEAQMH GGGHERGMRS GTLPTHQIVG MGEAFRLAKE EMADESRRVG ALRDRLLAGL STLDEVYVNG DLARRIPHNL NVSFNFVEGE SLIMGIKGVA VSSGSACTSA SLEPSYVLRA LGRSDELAHS SIRFTLGRFT TEAEVDSVIA QVRDTVGKLR ELSPLWDMHL EGVDLDTIEW AAH
NT Sequence
ATGCAATCCC GTCCGATCTA CATGGACTAC AGCGCGACGA CGCCGGTCGA TCCGCGCGTG GTCGCCAAGA TGGTGCCGTT CCTGCACGAG CAGTTCGGCA ACCCCGCCTC ACGCAGCCAC GCCTACGGCT GGGATGCCGA GCACGCCGTC GAGGAAGCGC GCGCGCACGT CGCCGCGCTG CTCGGCGCCG ATCCGCGCGA AATCGTGTGG ACGTCCGGCG CGACCGAAGG CAACAACCTC GCGATCAAGG GCGCCGCGCA CTTCTATCAA GGCAAGGGCA AGCATCTCGT GACGGTGAAG ACCGAGCACA AGGCCGTGCT CGATACGTGT CGTGAACTCG AACGCCAGGG TTTCGACGTC ACGTACCTCG ACGTGCGCGA AGACGGCCTG CTCGACCTCG ATGCGTTGCA GCAGGCGCTG CGCGCCGACA CGATCCTGGT GTCGGTGATG CTCGCGAACA ACGAAACGGG CGTGATCCAG CCGGTGGCCG ACATCGGTGC GCTGTGCCGC GCGCGCGGCA TCGTGTTTCA TTGCGATGCG GTGCAGGCGG CCGGCAAGAT TCCGGTGGAC GTGAATGCGC TGAACGTCGA CCTGCTGACG GTGACCGCGC ACAAGGTGTA CGGCCCGAAG GGGATCGGCG CGCTGTACGT GCGCCGCAAG CCGCGCGTGC GCCTCGAAGC GCAGATGCAC GGCGGCGGCC ACGAACGCGG GATGCGCTCC GGCACGCTGC CGACCCACCA GATCGTCGGG ATGGGCGAAG CGTTCCGGCT GGCGAAGGAA GAAATGGCGG ACGAAAGCCG CCGCGTCGGC GCGTTGCGCG ACCGGCTGCT GGCCGGCCTG TCGACGCTCG ACGAGGTTTA CGTGAACGGC GACCTCGCGC GCCGGATTCC GCACAACCTG AACGTCAGCT TCAATTTCGT CGAAGGCGAA TCGCTGATCA TGGGGATCAA GGGCGTCGCG GTGTCGTCAG GCTCCGCGTG CACGTCGGCG TCGCTGGAGC CGTCGTATGT GCTGCGCGCG CTCGGCCGCA GCGACGAGCT CGCGCACAGC TCGATCCGCT TCACGCTCGG CCGCTTCACG ACCGAAGCCG AGGTCGACAG CGTGATCGCG CAGGTGCGCG ACACGGTCGG CAAGCTGCGC GAATTGAGCC CGCTGTGGGA CATGCATCTC GAAGGGGTCG ACCTGGATAC GATCGAGTGG GCCGCGCAC
Details for BuceA.00081.b.A1.GE33191
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MQSRPIYMDY SATTPVDPRV VAKMVPFLHE QFGNPASRSH AYGWDAEHAV EEARAHVAAL LGADPREIVW TSGATEGNNL AIKGAAHFYQ GKGKHLVTVK TEHKAVLDTC RELERQGFDV TYLDVREDGL LDLDALQQAL RADTILVSVM LANNETGVIQ PVADIGALCR ARGIVFHCDA VQAAGKIPVD VNALNVDLLT VTAHKVYGPK GIGALYVRRK PRVRLEAQMH GGGHERGMRS GTLPTHQIVG MGEAFRLAKE EMADESRRVG ALRDRLLAGL STLDEVYVNG DLARRIPHNL NVSFNFVEGE SLIMGIKGVA VSSGSACTSA SLEPSYVLRA LGRSDELAHS SIRFTLGRFT TEAEVDSVIA QVRDTVGKLR ELSPLWDMHL EGVDLDTIEW AAH
Validated NT Sequence
agtgcgcggc ccactcgatc gtatccaggt cgaccccttc gagatgcatg tcccacagcg ggctcaattc gcgcagcttg ccgaccgtgt cgcgcacctg cgcgatcacg ctgtcgacct cggcttcggt cgtgaagcgg ccgagcgtga agcggatcga gctgtgcgcg agctcgtcgc tgcggccgag cgcgcgcagc acatacgacg gctccagcga cgccgacgtg cacgcggagc ctgacgacac cgcgacgccc ttgatcccca tgatcagcga ttcgccttcg acgaaattga agctgacgtt caggttgtgc ggaatccggc gcgcgaggtc gccgttcacg taaacctcgt cgagcgtcga caggccggcc agcagccggt cgcgcaacgc gccgacgcgg cggctttcgt ccgccatttc ttccttcgcc agccggaacg cttcgcccat cccgacgatc tggtgggtcg gcagcgtgcc ggagcgcatc ccgcgttcgt ggccgccgcc gtgcatctgc gcttcgaggc gcacgcgcgg cttgcggcgc acgtacagcg cgccgatccc cttcgggccg tacaccttgt gcgcggtcac cgtcagcagg tcgacgttca gcgcattcac gtccaccgga atcttgccgg ccgcctgcac cgcatcgcaa tgaaacacga tgccgcgcgc gcggcacagc gcaccgatgt cggccaccgg ctggatcacg cccgtttcgt tgttcgcgag catcaccgac accaggatcg tgtcggcgcg cagcgcctgc tgcaacgcat cgaggtcgag caggccgtct tcgcgcacgt cgaggtacgt gacgtcgaaa ccctggcgtt cgagttcacg acacgtatcg agcacggcct tgtgctcggt cttcaccgtc acgagatgct tgcccttgcc ttgatagaag tgcgcggcgc ccttgatcgc gaggttgttg ccttcggtcg cgccggacgt ccacacgatt tcgcgcggat cggcgccgag cagcgcggcg acgtgcgcgc gcgcttcctc gacggcgtgc tcggcatccc agccgtaggc gtggctgcgt gaggcggggt tgccgaactg ctcgtgcagg aacggcacca tcttggcgac cacgcgcgga tcgaccggcg tcgtcgcgct gtagtccatg tagatcggac gggattgcat cgaaccagga ccctgggtct gagcttccag ggtaccca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQSRPIYMD YSATTPVDPR VVAKMVPFLH EQFGNPASRS HAYGWDAEHA VEEARAHVAA LLGADPREIV WTSGATEGNN LAIKGAAHFY QGKGKHLVTV KTEHKAVLDT CRELERQGFD VTYLDVREDG LLDLDALQQA LRADTILVSV MLANNETGVI QPVADIGALC RARGIVFHCD AVQAAGKIPV DVNALNVDLL TVTAHKVYGP KGIGALYVRR KPRVRLEAQM HGGGHERGMR SGTLPTHQIV GMGEAFRLAK EEMADESRRV GALRDRLLAG LSTLDEVYVN GDLARRIPHN LNVSFNFVEG ESLIMGIKGV AVSSGSACTS ASLEPSYVLR ALGRSDELAH SSIRFTLGRF TTEAEVDSVI AQVRDTVGKL RELSPLWDMH LEGVDLDTIE WAAH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaatcccgt ccgatctaca tggactacag cgcgacgacg ccggtcgatc cgcgcgtggt cgccaagatg gtgccgttcc tgcacgagca gttcggcaac cccgcctcac gcagccacgc ctacggctgg gatgccgagc acgccgtcga ggaagcgcgc gcgcacgtcg ccgcgctgct cggcgccgat ccgcgcgaaa tcgtgtggac gtccggcgcg accgaaggca acaacctcgc gatcaagggc gccgcgcact tctatcaagg caagggcaag catctcgtga cggtgaagac cgagcacaag gccgtgctcg atacgtgtcg tgaactcgaa cgccagggtt tcgacgtcac gtacctcgac gtgcgcgaag acggcctgct cgacctcgat gcgttgcagc aggcgctgcg cgccgacacg atcctggtgt cggtgatgct cgcgaacaac gaaacgggcg tgatccagcc ggtggccgac atcggtgcgc tgtgccgcgc gcgcggcatc gtgtttcatt gcgatgcggt gcaggcggcc ggcaagattc cggtggacgt gaatgcgctg aacgtcgacc tgctgacggt gaccgcgcac aaggtgtacg gcccgaaggg gatcggcgcg ctgtacgtgc gccgcaagcc gcgcgtgcgc ctcgaagcgc agatgcacgg cggcggccac gaacgcggga tgcgctccgg cacgctgccg acccaccaga tcgtcgggat gggcgaagcg ttccggctgg cgaaggaaga aatggcggac gaaagccgcc gcgtcggcgc gttgcgcgac cggctgctgg ccggcctgtc gacgctcgac gaggtttacg tgaacggcga cctcgcgcgc cggattccgc acaacctgaa cgtcagcttc aatttcgtcg aaggcgaatc gctgatcatg gggatcaagg gcgtcgcggt gtcgtcaggc tccgcgtgca cgtcggcgtc gctggagccg tcgtatgtgc tgcgcgcgct cggccgcagc gacgagctcg cgcacagctc gatccgcttc acgctcggcc gcttcacgac cgaagccgag gtcgacagcg tgatcgcgca ggtgcgcgac acggtcggca agctgcgcga attgagcccg ctgtgggaca tgcatctcga aggggtcgac ctggatacga tcgagtgggc cgcgcactaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BuceA.00081.b.A1.PW36053
PURIFICATION DATe: 1/13/2012
CONCENTRATION: 28.09mg/ml
OBSERVED MW: 44kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MQSRPIYMDY SATTPVDPRV VAKMVPFLHE QFGNPASRSH AYGWDAEHAV EEARAHVAAL LGADPREIVW TSGATEGNNL AIKGAAHFYQ GKGKHLVTVK TEHKAVLDTC RELERQGFDV TYLDVREDGL LDLDALQQAL RADTILVSVM LANNETGVIQ PVADIGALCR ARGIVFHCDA VQAAGKIPVD VNALNVDLLT VTAHKVYGPK GIGALYVRRK PRVRLEAQMH GGGHERGMRS GTLPTHQIVG MGEAFRLAKE EMADESRRVG ALRDRLLAGL STLDEVYVNG DLARRIPHNL NVSFNFVEGE SLIMGIKGVA VSSGSACTSA SLEPSYVLRA LGRSDELAHS SIRFTLGRFT TEAEVDSVIA QVRDTVGKLR ELSPLWDMHL EGVDLDTIEW AAH
Validated NT Sequence
agtgcgcggc ccactcgatc gtatccaggt cgaccccttc gagatgcatg tcccacagcg ggctcaattc gcgcagcttg ccgaccgtgt cgcgcacctg cgcgatcacg ctgtcgacct cggcttcggt cgtgaagcgg ccgagcgtga agcggatcga gctgtgcgcg agctcgtcgc tgcggccgag cgcgcgcagc acatacgacg gctccagcga cgccgacgtg cacgcggagc ctgacgacac cgcgacgccc ttgatcccca tgatcagcga ttcgccttcg acgaaattga agctgacgtt caggttgtgc ggaatccggc gcgcgaggtc gccgttcacg taaacctcgt cgagcgtcga caggccggcc agcagccggt cgcgcaacgc gccgacgcgg cggctttcgt ccgccatttc ttccttcgcc agccggaacg cttcgcccat cccgacgatc tggtgggtcg gcagcgtgcc ggagcgcatc ccgcgttcgt ggccgccgcc gtgcatctgc gcttcgaggc gcacgcgcgg cttgcggcgc acgtacagcg cgccgatccc cttcgggccg tacaccttgt gcgcggtcac cgtcagcagg tcgacgttca gcgcattcac gtccaccgga atcttgccgg ccgcctgcac cgcatcgcaa tgaaacacga tgccgcgcgc gcggcacagc gcaccgatgt cggccaccgg ctggatcacg cccgtttcgt tgttcgcgag catcaccgac accaggatcg tgtcggcgcg cagcgcctgc tgcaacgcat cgaggtcgag caggccgtct tcgcgcacgt cgaggtacgt gacgtcgaaa ccctggcgtt cgagttcacg acacgtatcg agcacggcct tgtgctcggt cttcaccgtc acgagatgct tgcccttgcc ttgatagaag tgcgcggcgc ccttgatcgc gaggttgttg ccttcggtcg cgccggacgt ccacacgatt tcgcgcggat cggcgccgag cagcgcggcg acgtgcgcgc gcgcttcctc gacggcgtgc tcggcatccc agccgtaggc gtggctgcgt gaggcggggt tgccgaactg ctcgtgcagg aacggcacca tcttggcgac cacgcgcgga tcgaccggcg tcgtcgcgct gtagtccatg tagatcggac gggattgcat cgaaccagga ccctgggtct gagcttccag ggtaccca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQSRPIYMD YSATTPVDPR VVAKMVPFLH EQFGNPASRS HAYGWDAEHA VEEARAHVAA LLGADPREIV WTSGATEGNN LAIKGAAHFY QGKGKHLVTV KTEHKAVLDT CRELERQGFD VTYLDVREDG LLDLDALQQA LRADTILVSV MLANNETGVI QPVADIGALC RARGIVFHCD AVQAAGKIPV DVNALNVDLL TVTAHKVYGP KGIGALYVRR KPRVRLEAQM HGGGHERGMR SGTLPTHQIV GMGEAFRLAK EEMADESRRV GALRDRLLAG LSTLDEVYVN GDLARRIPHN LNVSFNFVEG ESLIMGIKGV AVSSGSACTS ASLEPSYVLR ALGRSDELAH SSIRFTLGRF TTEAEVDSVI AQVRDTVGKL RELSPLWDMH LEGVDLDTIE WAAH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAATCCCGT CCGATCTACA TGGACTACAG CGCGACGACG CCGGTCGATC CGCGCGTGGT CGCCAAGATG GTGCCGTTCC TGCACGAGCA GTTCGGCAAC CCCGCCTCAC GCAGCCACGC CTACGGCTGG GATGCCGAGC ACGCCGTCGA GGAAGCGCGC GCGCACGTCG CCGCGCTGCT CGGCGCCGAT CCGCGCGAAA TCGTGTGGAC GTCCGGCGCG ACCGAAGGCA ACAACCTCGC GATCAAGGGC GCCGCGCACT TCTATCAAGG CAAGGGCAAG CATCTCGTGA CGGTGAAGAC CGAGCACAAG GCCGTGCTCG ATACGTGTCG TGAACTCGAA CGCCAGGGTT TCGACGTCAC GTACCTCGAC GTGCGCGAAG ACGGCCTGCT CGACCTCGAT GCGTTGCAGC AGGCGCTGCG CGCCGACACG ATCCTGGTGT CGGTGATGCT CGCGAACAAC GAAACGGGCG TGATCCAGCC GGTGGCCGAC ATCGGTGCGC TGTGCCGCGC GCGCGGCATC GTGTTTCATT GCGATGCGGT GCAGGCGGCC GGCAAGATTC CGGTGGACGT GAATGCGCTG AACGTCGACC TGCTGACGGT GACCGCGCAC AAGGTGTACG GCCCGAAGGG GATCGGCGCG CTGTACGTGC GCCGCAAGCC GCGCGTGCGC CTCGAAGCGC AGATGCACGG CGGCGGCCAC GAACGCGGGA TGCGCTCCGG CACGCTGCCG ACCCACCAGA TCGTCGGGAT GGGCGAAGCG TTCCGGCTGG CGAAGGAAGA AATGGCGGAC GAAAGCCGCC GCGTCGGCGC GTTGCGCGAC CGGCTGCTGG CCGGCCTGTC GACGCTCGAC GAGGTTTACG TGAACGGCGA CCTCGCGCGC CGGATTCCGC ACAACCTGAA CGTCAGCTTC AATTTCGTCG AAGGCGAATC GCTGATCATG GGGATCAAGG GCGTCGCGGT GTCGTCAGGC TCCGCGTGCA CGTCGGCGTC GCTGGAGCCG TCGTATGTGC TGCGCGCGCT CGGCCGCAGC GACGAGCTCG CGCACAGCTC GATCCGCTTC ACGCTCGGCC GCTTCACGAC CGAAGCCGAG GTCGACAGCG TGATCGCGCA GGTGCGCGAC ACGGTCGGCA AGCTGCGCGA ATTGAGCCCG CTGTGGGACA TGCATCTCGA AGGGGTCGAC CTGGATACGA TCGAGTGGGC CGCGCACTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA