BuceA.00077.a
Putative hydrolase
| CENTER ID: | BuceA.00077.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00077.a.B1.GE36649 | Full length( BuceA.00077.a ) | 1 | 433 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00077.a.B1.PW37289 | Full length( BuceA.00077.a ) | 1 | 433 | ||
| BuceA.00077.a.B1.PW37370 | Full length( BuceA.00077.a ) | 1 | 433 |
Structures
4QKU
DEPOSITED: 6/9/2014
DETERMINATION: XRay
CLONE: BuceA.00077.a.B1.GE36649
PROTEIN: BuceA.00077.a.B1.PW37289
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216591.5.peg.3304 |
| RefSeq: | YP_002232292.1 |
| UniProt: | B4ECX4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDTQDWRAT LDPARKSWIE TANDPACDFP IQNLPFGIFS DAKGARRPGV ALGDQIVDLA ALARAGLVTL PAGADVLAAP TLNAFIALGR DAWRSVRVQL SALFSRDDAT LRDDAALRAQ VLVAQRDATL HLPVEIPGYT DFYSSKEHAT NVGSMFRDPK NALLPNWSEM PIGYNGRASS VVVSGTPVRR PNGQLKLPDQ ERPVFGACRK LDIELETGFI VGKGNALGEP IACEDAEAHI FGMVLLNDWS ARDIQQWEYV PLGPFNSKGF ATTISPWIVT LDALEPFRVA QPEQSPQPLA YLRHAGKHAF DIALEVTLRA EGAAEATSIC RTNFRHMYWT MAQQLAHHTV AGCNTRVGDL MGSGTISGPT KDSFGSLLEL TWNGKEPVAL NGGGSRTFIE DGDELTLAGW CQGDGYRVGF GTCAGRILPA RSA
NT Sequence
ATGAGCGACA CCCAAGACTG GCGCGCGACG CTCGACCCGG CTCGCAAGAG CTGGATCGAG ACCGCGAACG ATCCCGCCTG CGATTTCCCG ATCCAGAACC TGCCGTTCGG CATCTTCAGC GATGCGAAGG GCGCGCGCCG CCCGGGCGTC GCGCTGGGCG ACCAGATCGT CGATCTCGCG GCGCTCGCGC GTGCGGGCCT CGTCACGCTG CCGGCCGGCG CCGACGTGCT CGCCGCACCG ACGCTCAATG CGTTCATCGC GCTCGGCCGC GACGCGTGGC GCAGCGTGCG CGTGCAGCTG TCGGCGCTGT TCTCGCGTGA CGACGCGACA CTGCGTGACG ATGCGGCGCT GCGCGCGCAG GTGCTCGTCG CGCAGCGCGA CGCGACGCTG CATCTGCCGG TCGAGATTCC CGGCTACACC GATTTCTATT CGTCGAAGGA ACACGCGACC AACGTCGGCT CGATGTTCCG CGATCCGAAG AATGCGCTGC TGCCGAACTG GTCGGAGATG CCGATCGGCT ACAACGGCCG CGCGTCGTCG GTGGTCGTGA GCGGCACGCC GGTGCGGCGC CCGAACGGGC AACTGAAGCT GCCCGACCAG GAGCGCCCGG TGTTCGGCGC GTGCCGCAAG CTCGACATCG AACTGGAAAC GGGCTTCATC GTCGGCAAGG GCAACGCGCT CGGCGAGCCG ATCGCGTGCG AGGACGCGGA AGCGCATATC TTCGGGATGG TGCTGCTGAA CGACTGGAGC GCGCGCGACA TCCAGCAGTG GGAATACGTG CCGCTCGGCC CGTTCAACTC GAAGGGGTTC GCAACGACGA TCTCGCCGTG GATCGTCACG CTGGATGCGC TCGAACCGTT CCGCGTCGCG CAGCCCGAGC AGTCGCCGCA GCCGCTCGCG TATCTGCGGC ATGCGGGCAA GCATGCGTTC GACATCGCGC TGGAAGTGAC GCTGCGCGCC GAAGGTGCGG CCGAGGCCAC GTCGATCTGC CGCACGAACT TCAGGCACAT GTACTGGACG ATGGCGCAGC AGCTCGCGCA TCACACGGTC GCCGGCTGCA ACACGCGCGT GGGCGACCTG ATGGGTTCGG GCACGATCAG CGGGCCGACG AAGGATTCGT TCGGCAGCCT GCTCGAACTG ACGTGGAACG GCAAGGAGCC GGTCGCGCTG AACGGCGGCG GCAGCCGCAC GTTCATCGAG GACGGCGACG AGCTGACGCT CGCCGGCTGG TGCCAGGGAG ATGGGTATCG CGTCGGCTTT GGCACGTGTG CGGGCAGGAT CTTGCCGGCG CGGAGTGCG
Details for BuceA.00077.a.B1.GE36649
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 2/14/2013 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 23 |
| PERCENT COVERAGE: | 78 |
Validated AA Sequence
MRELLRHRPV HVPEVRAADR RGLGRTFGAQ RHFQRDVERM LARMPQIRER LRRLLGLRDA ERFERIQRDD PRRDRRCEPL RVERAERHVF PLLDVARAPV VQQHHPEDMR FRVLARDRLA ERVALADDEA RFQFDVELAA RAEHRALLVG QLQLPVRAPH RRAAHDHRRR AAVVADRHLR PVRQQRILRI AEHRADVGRV FLRRIEIGVA GNLDRQMQRR VALRDEHLRA QRRIVTQCRV VTREQRRQLH AHAAPRVAAE RDERIERRCG EHVGAGRQRD EARTRERREI DDLVAQRDAR AARALRIAED AERQVLDREI AGGIVRGLDP ALASRVERRA PVLG
Validated NT Sequence
atcttactca cttacgcact ccgcgccggc aagatcctgc ccgcacacgt gccaaagccg acgcgatacc catctccctg gcaccagccg gcgagcgtca gctcgtcgcc gtcctcgatg aacgtgcggc tgccgccgcc gttcagcgcg accggctcct tgccgttcca cgtcagttcg agcaggctgc cgaacgaatc cttcgtcggc ccgctgatcg tgcccgaacc catcaggtcg cccacgcgcg tgttgcagcc ggcgaccgtg tgatgcgcga gctgctgcgc catcgtccag tacatgtgcc tgaagttcgt gcggcagatc gacgtggcct cggccgcacc ttcggcgcgc agcgtcactt ccagcgcgat gtcgaacgca tgcttgcccg catgccgcag atacgcgagc ggctgcggcg actgctcggg ctgcgcgacg cggaacggtt cgagcgcatc cagcgtgacg atccacggcg agatcgtcgt tgcgaacccc ttcgagttga acgggccgag cggcacgtat tcccactgct ggatgtcgcg cgcgctccag tcgttcagca gcaccatccc gaagatatgc gcttccgcgt cctcgcacgc gatcggctcg ccgagcgcgt tgcccttgcc gacgatgaag cccgtttcca gttcgatgtc gagcttgcgg cacgcgccga acaccgggcg ctcctggtcg ggcagcttca gttgcccgtt cgggcgccgc accggcgtgc cgctcacgac caccgacgac gcgcggccgt tgtagccgat cggcatctcc gaccagttcg gcagcagcgc attcttcgga tcgcggaaca tcgagccgac gttggtcgcg tgttccttcg acgaatagaa atcggtgtag ccgggaatct cgaccggcag atgcagcgtc gcgtcgcgct gcgcgacgag cacctgcgcg cgcagcgccg catcgtcacg cagtgtcgcg tcgtcacgcg agaacagcgc cgacagctgc acgcgcacgc tgcgccacgc gtcgcggccg agcgcgatga acgcattgag cgtcggtgcg gcgagcacgt cggcgccggc cggcagcgtg acgaggcccg cacgcgcgag cgccgcgaga tcgacgatct ggtcgcccag cgcgacgccc gggcggcgcg cgcccttcgc atcgctgaag atgccgaacg gcaggttctg gatcgggaaa tcgcaggcgg gatcgttcgc ggtctcgatc cagctcttgc gagccgggtc gagcgtcgcg cgccagtctt gggtgt
Expected Protein Sequence
MAHHHHHHMS DTQDWRATLD PARKSWIETA NDPACDFPIQ NLPFGIFSDA KGARRPGVAL GDQIVDLAAL ARAGLVTLPA GADVLAAPTL NAFIALGRDA WRSVRVQLSA LFSRDDATLR DDAALRAQVL VAQRDATLHL PVEIPGYTDF YSSKEHATNV GSMFRDPKNA LLPNWSEMPI GYNGRASSVV VSGTPVRRPN GQLKLPDQER PVFGACRKLD IELETGFIVG KGNALGEPIA CEDAEAHIFG MVLLNDWSAR DIQQWEYVPL GPFNSKGFAT TISPWIVTLD ALEPFRVAQP EQSPQPLAYL RHAGKHAFDI ALEVTLRAEG AAEATSICRT NFRHMYWTMA QQLAHHTVAG CNTRVGDLMG SGTISGPTKD SFGSLLELTW NGKEPVALNG GGSRTFIEDG DELTLAGWCQ GDGYRVGFGT CAGRILPARS A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg acacccaaga ctggcgcgcg acgctcgacc cggctcgcaa gagctggatc gagaccgcga acgatcccgc ctgcgatttc ccgatccaga acctgccgtt cggcatcttc agcgatgcga agggcgcgcg ccgcccgggc gtcgcgctgg gcgaccagat cgtcgatctc gcggcgctcg cgcgtgcggg cctcgtcacg ctgccggccg gcgccgacgt gctcgccgca ccgacgctca atgcgttcat cgcgctcggc cgcgacgcgt ggcgcagcgt gcgcgtgcag ctgtcggcgc tgttctcgcg tgacgacgcg acactgcgtg acgatgcggc gctgcgcgcg caggtgctcg tcgcgcagcg cgacgcgacg ctgcatctgc cggtcgagat tcccggctac accgatttct attcgtcgaa ggaacacgcg accaacgtcg gctcgatgtt ccgcgatccg aagaatgcgc tgctgccgaa ctggtcggag atgccgatcg gctacaacgg ccgcgcgtcg tcggtggtcg tgagcggcac gccggtgcgg cgcccgaacg ggcaactgaa gctgcccgac caggagcgcc cggtgttcgg cgcgtgccgc aagctcgaca tcgaactgga aacgggcttc atcgtcggca agggcaacgc gctcggcgag ccgatcgcgt gcgaggacgc ggaagcgcat atcttcggga tggtgctgct gaacgactgg agcgcgcgcg acatccagca gtgggaatac gtgccgctcg gcccgttcaa ctcgaagggg ttcgcaacga cgatctcgcc gtggatcgtc acgctggatg cgctcgaacc gttccgcgtc gcgcagcccg agcagtcgcc gcagccgctc gcgtatctgc ggcatgcggg caagcatgcg ttcgacatcg cgctggaagt gacgctgcgc gccgaaggtg cggccgaggc cacgtcgatc tgccgcacga acttcaggca catgtactgg acgatggcgc agcagctcgc gcatcacacg gtcgccggct gcaacacgcg cgtgggcgac ctgatgggtt cgggcacgat cagcgggccg acgaaggatt cgttcggcag cctgctcgaa ctgacgtgga acggcaagga gccggtcgcg ctgaacggcg gcggcagccg cacgttcatc gaggacggcg acgagctgac gctcgccggc tggtgccagg gagatgggta tcgcgtcggc tttggcacgt gtgcgggcag gatcttgccg gcgcggagtg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuceA.00077.a.B1.PW37289
| PURIFICATION DATe: | 5/13/2013 |
| CONCENTRATION: | 41.6mg/ml |
| OBSERVED MW: | 47kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 23 |
| PERCENT COVERAGE: | 78 |
Protocol Notes
notes unavailable
Validated AA Sequence
MRELLRHRPV HVPEVRAADR RGLGRTFGAQ RHFQRDVERM LARMPQIRER LRRLLGLRDA ERFERIQRDD PRRDRRCEPL RVERAERHVF PLLDVARAPV VQQHHPEDMR FRVLARDRLA ERVALADDEA RFQFDVELAA RAEHRALLVG QLQLPVRAPH RRAAHDHRRR AAVVADRHLR PVRQQRILRI AEHRADVGRV FLRRIEIGVA GNLDRQMQRR VALRDEHLRA QRRIVTQCRV VTREQRRQLH AHAAPRVAAE RDERIERRCG EHVGAGRQRD EARTRERREI DDLVAQRDAR AARALRIAED AERQVLDREI AGGIVRGLDP ALASRVERRA PVLG
Validated NT Sequence
atcttactca cttacgcact ccgcgccggc aagatcctgc ccgcacacgt gccaaagccg acgcgatacc catctccctg gcaccagccg gcgagcgtca gctcgtcgcc gtcctcgatg aacgtgcggc tgccgccgcc gttcagcgcg accggctcct tgccgttcca cgtcagttcg agcaggctgc cgaacgaatc cttcgtcggc ccgctgatcg tgcccgaacc catcaggtcg cccacgcgcg tgttgcagcc ggcgaccgtg tgatgcgcga gctgctgcgc catcgtccag tacatgtgcc tgaagttcgt gcggcagatc gacgtggcct cggccgcacc ttcggcgcgc agcgtcactt ccagcgcgat gtcgaacgca tgcttgcccg catgccgcag atacgcgagc ggctgcggcg actgctcggg ctgcgcgacg cggaacggtt cgagcgcatc cagcgtgacg atccacggcg agatcgtcgt tgcgaacccc ttcgagttga acgggccgag cggcacgtat tcccactgct ggatgtcgcg cgcgctccag tcgttcagca gcaccatccc gaagatatgc gcttccgcgt cctcgcacgc gatcggctcg ccgagcgcgt tgcccttgcc gacgatgaag cccgtttcca gttcgatgtc gagcttgcgg cacgcgccga acaccgggcg ctcctggtcg ggcagcttca gttgcccgtt cgggcgccgc accggcgtgc cgctcacgac caccgacgac gcgcggccgt tgtagccgat cggcatctcc gaccagttcg gcagcagcgc attcttcgga tcgcggaaca tcgagccgac gttggtcgcg tgttccttcg acgaatagaa atcggtgtag ccgggaatct cgaccggcag atgcagcgtc gcgtcgcgct gcgcgacgag cacctgcgcg cgcagcgccg catcgtcacg cagtgtcgcg tcgtcacgcg agaacagcgc cgacagctgc acgcgcacgc tgcgccacgc gtcgcggccg agcgcgatga acgcattgag cgtcggtgcg gcgagcacgt cggcgccggc cggcagcgtg acgaggcccg cacgcgcgag cgccgcgaga tcgacgatct ggtcgcccag cgcgacgccc gggcggcgcg cgcccttcgc atcgctgaag atgccgaacg gcaggttctg gatcgggaaa tcgcaggcgg gatcgttcgc ggtctcgatc cagctcttgc gagccgggtc gagcgtcgcg cgccagtctt gggtgt
Expressed Protein Sequence
MAHHHHHHMS DTQDWRATLD PARKSWIETA NDPACDFPIQ NLPFGIFSDA KGARRPGVAL GDQIVDLAAL ARAGLVTLPA GADVLAAPTL NAFIALGRDA WRSVRVQLSA LFSRDDATLR DDAALRAQVL VAQRDATLHL PVEIPGYTDF YSSKEHATNV GSMFRDPKNA LLPNWSEMPI GYNGRASSVV VSGTPVRRPN GQLKLPDQER PVFGACRKLD IELETGFIVG KGNALGEPIA CEDAEAHIFG MVLLNDWSAR DIQQWEYVPL GPFNSKGFAT TISPWIVTLD ALEPFRVAQP EQSPQPLAYL RHAGKHAFDI ALEVTLRAEG AAEATSICRT NFRHMYWTMA QQLAHHTVAG CNTRVGDLMG SGTISGPTKD SFGSLLELTW NGKEPVALNG GGSRTFIEDG DELTLAGWCQ GDGYRVGFGT CAGRILPARS A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG ACACCCAAGA CTGGCGCGCG ACGCTCGACC CGGCTCGCAA GAGCTGGATC GAGACCGCGA ACGATCCCGC CTGCGATTTC CCGATCCAGA ACCTGCCGTT CGGCATCTTC AGCGATGCGA AGGGCGCGCG CCGCCCGGGC GTCGCGCTGG GCGACCAGAT CGTCGATCTC GCGGCGCTCG CGCGTGCGGG CCTCGTCACG CTGCCGGCCG GCGCCGACGT GCTCGCCGCA CCGACGCTCA ATGCGTTCAT CGCGCTCGGC CGCGACGCGT GGCGCAGCGT GCGCGTGCAG CTGTCGGCGC TGTTCTCGCG TGACGACGCG ACACTGCGTG ACGATGCGGC GCTGCGCGCG CAGGTGCTCG TCGCGCAGCG CGACGCGACG CTGCATCTGC CGGTCGAGAT TCCCGGCTAC ACCGATTTCT ATTCGTCGAA GGAACACGCG ACCAACGTCG GCTCGATGTT CCGCGATCCG AAGAATGCGC TGCTGCCGAA CTGGTCGGAG ATGCCGATCG GCTACAACGG CCGCGCGTCG TCGGTGGTCG TGAGCGGCAC GCCGGTGCGG CGCCCGAACG GGCAACTGAA GCTGCCCGAC CAGGAGCGCC CGGTGTTCGG CGCGTGCCGC AAGCTCGACA TCGAACTGGA AACGGGCTTC ATCGTCGGCA AGGGCAACGC GCTCGGCGAG CCGATCGCGT GCGAGGACGC GGAAGCGCAT ATCTTCGGGA TGGTGCTGCT GAACGACTGG AGCGCGCGCG ACATCCAGCA GTGGGAATAC GTGCCGCTCG GCCCGTTCAA CTCGAAGGGG TTCGCAACGA CGATCTCGCC GTGGATCGTC ACGCTGGATG CGCTCGAACC GTTCCGCGTC GCGCAGCCCG AGCAGTCGCC GCAGCCGCTC GCGTATCTGC GGCATGCGGG CAAGCATGCG TTCGACATCG CGCTGGAAGT GACGCTGCGC GCCGAAGGTG CGGCCGAGGC CACGTCGATC TGCCGCACGA ACTTCAGGCA CATGTACTGG ACGATGGCGC AGCAGCTCGC GCATCACACG GTCGCCGGCT GCAACACGCG CGTGGGCGAC CTGATGGGTT CGGGCACGAT CAGCGGGCCG ACGAAGGATT CGTTCGGCAG CCTGCTCGAA CTGACGTGGA ACGGCAAGGA GCCGGTCGCG CTGAACGGCG GCGGCAGCCG CACGTTCATC GAGGACGGCG ACGAGCTGAC GCTCGCCGGC TGGTGCCAGG GAGATGGGTA TCGCGTCGGC TTTGGCACGT GTGCGGGCAG GATCTTGCCG GCGCGGAGTG CGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT
Details for BuceA.00077.a.B1.PW37370
| PURIFICATION DATe: | 10/1/2013 |
| CONCENTRATION: | 47.3mg/ml |
| OBSERVED MW: | 46kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 23 |
| PERCENT COVERAGE: | 78 |
Protocol Notes
notes unavailable
Validated AA Sequence
MRELLRHRPV HVPEVRAADR RGLGRTFGAQ RHFQRDVERM LARMPQIRER LRRLLGLRDA ERFERIQRDD PRRDRRCEPL RVERAERHVF PLLDVARAPV VQQHHPEDMR FRVLARDRLA ERVALADDEA RFQFDVELAA RAEHRALLVG QLQLPVRAPH RRAAHDHRRR AAVVADRHLR PVRQQRILRI AEHRADVGRV FLRRIEIGVA GNLDRQMQRR VALRDEHLRA QRRIVTQCRV VTREQRRQLH AHAAPRVAAE RDERIERRCG EHVGAGRQRD EARTRERREI DDLVAQRDAR AARALRIAED AERQVLDREI AGGIVRGLDP ALASRVERRA PVLG
Validated NT Sequence
atcttactca cttacgcact ccgcgccggc aagatcctgc ccgcacacgt gccaaagccg acgcgatacc catctccctg gcaccagccg gcgagcgtca gctcgtcgcc gtcctcgatg aacgtgcggc tgccgccgcc gttcagcgcg accggctcct tgccgttcca cgtcagttcg agcaggctgc cgaacgaatc cttcgtcggc ccgctgatcg tgcccgaacc catcaggtcg cccacgcgcg tgttgcagcc ggcgaccgtg tgatgcgcga gctgctgcgc catcgtccag tacatgtgcc tgaagttcgt gcggcagatc gacgtggcct cggccgcacc ttcggcgcgc agcgtcactt ccagcgcgat gtcgaacgca tgcttgcccg catgccgcag atacgcgagc ggctgcggcg actgctcggg ctgcgcgacg cggaacggtt cgagcgcatc cagcgtgacg atccacggcg agatcgtcgt tgcgaacccc ttcgagttga acgggccgag cggcacgtat tcccactgct ggatgtcgcg cgcgctccag tcgttcagca gcaccatccc gaagatatgc gcttccgcgt cctcgcacgc gatcggctcg ccgagcgcgt tgcccttgcc gacgatgaag cccgtttcca gttcgatgtc gagcttgcgg cacgcgccga acaccgggcg ctcctggtcg ggcagcttca gttgcccgtt cgggcgccgc accggcgtgc cgctcacgac caccgacgac gcgcggccgt tgtagccgat cggcatctcc gaccagttcg gcagcagcgc attcttcgga tcgcggaaca tcgagccgac gttggtcgcg tgttccttcg acgaatagaa atcggtgtag ccgggaatct cgaccggcag atgcagcgtc gcgtcgcgct gcgcgacgag cacctgcgcg cgcagcgccg catcgtcacg cagtgtcgcg tcgtcacgcg agaacagcgc cgacagctgc acgcgcacgc tgcgccacgc gtcgcggccg agcgcgatga acgcattgag cgtcggtgcg gcgagcacgt cggcgccggc cggcagcgtg acgaggcccg cacgcgcgag cgccgcgaga tcgacgatct ggtcgcccag cgcgacgccc gggcggcgcg cgcccttcgc atcgctgaag atgccgaacg gcaggttctg gatcgggaaa tcgcaggcgg gatcgttcgc ggtctcgatc cagctcttgc gagccgggtc gagcgtcgcg cgccagtctt gggtgt
Expressed Protein Sequence
MAHHHHHHMS DTQDWRATLD PARKSWIETA NDPACDFPIQ NLPFGIFSDA KGARRPGVAL GDQIVDLAAL ARAGLVTLPA GADVLAAPTL NAFIALGRDA WRSVRVQLSA LFSRDDATLR DDAALRAQVL VAQRDATLHL PVEIPGYTDF YSSKEHATNV GSMFRDPKNA LLPNWSEMPI GYNGRASSVV VSGTPVRRPN GQLKLPDQER PVFGACRKLD IELETGFIVG KGNALGEPIA CEDAEAHIFG MVLLNDWSAR DIQQWEYVPL GPFNSKGFAT TISPWIVTLD ALEPFRVAQP EQSPQPLAYL RHAGKHAFDI ALEVTLRAEG AAEATSICRT NFRHMYWTMA QQLAHHTVAG CNTRVGDLMG SGTISGPTKD SFGSLLELTW NGKEPVALNG GGSRTFIEDG DELTLAGWCQ GDGYRVGFGT CAGRILPARS A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG ACACCCAAGA CTGGCGCGCG ACGCTCGACC CGGCTCGCAA GAGCTGGATC GAGACCGCGA ACGATCCCGC CTGCGATTTC CCGATCCAGA ACCTGCCGTT CGGCATCTTC AGCGATGCGA AGGGCGCGCG CCGCCCGGGC GTCGCGCTGG GCGACCAGAT CGTCGATCTC GCGGCGCTCG CGCGTGCGGG CCTCGTCACG CTGCCGGCCG GCGCCGACGT GCTCGCCGCA CCGACGCTCA ATGCGTTCAT CGCGCTCGGC CGCGACGCGT GGCGCAGCGT GCGCGTGCAG CTGTCGGCGC TGTTCTCGCG TGACGACGCG ACACTGCGTG ACGATGCGGC GCTGCGCGCG CAGGTGCTCG TCGCGCAGCG CGACGCGACG CTGCATCTGC CGGTCGAGAT TCCCGGCTAC ACCGATTTCT ATTCGTCGAA GGAACACGCG ACCAACGTCG GCTCGATGTT CCGCGATCCG AAGAATGCGC TGCTGCCGAA CTGGTCGGAG ATGCCGATCG GCTACAACGG CCGCGCGTCG TCGGTGGTCG TGAGCGGCAC GCCGGTGCGG CGCCCGAACG GGCAACTGAA GCTGCCCGAC CAGGAGCGCC CGGTGTTCGG CGCGTGCCGC AAGCTCGACA TCGAACTGGA AACGGGCTTC ATCGTCGGCA AGGGCAACGC GCTCGGCGAG CCGATCGCGT GCGAGGACGC GGAAGCGCAT ATCTTCGGGA TGGTGCTGCT GAACGACTGG AGCGCGCGCG ACATCCAGCA GTGGGAATAC GTGCCGCTCG GCCCGTTCAA CTCGAAGGGG TTCGCAACGA CGATCTCGCC GTGGATCGTC ACGCTGGATG CGCTCGAACC GTTCCGCGTC GCGCAGCCCG AGCAGTCGCC GCAGCCGCTC GCGTATCTGC GGCATGCGGG CAAGCATGCG TTCGACATCG CGCTGGAAGT GACGCTGCGC GCCGAAGGTG CGGCCGAGGC CACGTCGATC TGCCGCACGA ACTTCAGGCA CATGTACTGG ACGATGGCGC AGCAGCTCGC GCATCACACG GTCGCCGGCT GCAACACGCG CGTGGGCGAC CTGATGGGTT CGGGCACGAT CAGCGGGCCG ACGAAGGATT CGTTCGGCAG CCTGCTCGAA CTGACGTGGA ACGGCAAGGA GCCGGTCGCG CTGAACGGCG GCGGCAGCCG CACGTTCATC GAGGACGGCG ACGAGCTGAC GCTCGCCGGC TGGTGCCAGG GAGATGGGTA TCGCGTCGGC TTTGGCACGT GTGCGGGCAG GATCTTGCCG GCGCGGAGTG CGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT