BuceA.00065.a

p-hydroxybenzoate hydroxylase

CENTER ID: BuceA.00065.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BuceA.00065.a.B1.GE36644 Full length( BuceA.00065.a ) 1 410
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3731
RefSeq: YP_002232690.1
UniProt: B4EGI1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRTQVAIIGA GPSGLLLSHL LRLQGVDSIL VEARSREYCE NRIRAGVLEQ GTVDTLNEAG LGDRMRREGL EHHGIELLFD GQRHRIDLSE LTGGRAITVY SQHEVVRDLI AAGVEHGHQM HFEVSDVALH DVESERPFVT FKHADGRADR IDCDYIAGCD GFHGIARQTI PADRLNTFER VYPFAWLGIL ADAAPSLDEL VYAHHDNGFA LFSMRSPTVT RLYLQCKPDE DLAEWSDARI WDELHTRFSN DTGWTPTEGR ITQKSVTPMR SFVSETMQHG RLFLAGDAAH IVPPTGAKGM NLAVADVRAL SRALGARYRT GDATPLERYS ATCLERVWRA EHFSYFMTNM LHASPDDSPF VNRLKFAELK YVTRSRAAAQ SLAENYVGLP FDDATASEAT RLDNALCATL
NT Sequence
ATGCGCACCC AAGTCGCGAT CATCGGCGCC GGTCCGTCCG GCCTTCTGCT TTCCCACCTG CTGCGCCTGC AAGGCGTCGA TTCCATCCTC GTCGAAGCGC GTTCGCGCGA ATACTGCGAG AACCGCATCC GCGCCGGCGT GCTCGAGCAG GGCACGGTCG ACACGCTGAA CGAAGCCGGC CTCGGCGACC GGATGCGTCG CGAAGGGCTC GAACATCACG GCATCGAGCT GCTGTTCGAC GGCCAGCGTC ACCGCATCGA CCTGTCCGAG CTGACCGGTG GGCGCGCGAT CACCGTCTAC AGCCAGCACG AAGTCGTACG CGACCTGATC GCGGCCGGCG TCGAGCACGG TCATCAGATG CACTTCGAAG TCAGCGACGT CGCGCTGCAC GATGTCGAAA GCGAGCGGCC GTTCGTCACG TTCAAGCATG CGGACGGTCG CGCCGACCGC ATCGACTGCG ACTACATCGC GGGCTGCGAC GGCTTCCACG GCATCGCGCG GCAGACGATT CCGGCCGACC GGCTGAACAC GTTCGAGCGC GTCTACCCGT TCGCGTGGCT CGGCATCCTC GCCGACGCGG CGCCGTCGCT CGACGAACTC GTGTACGCAC ACCACGACAA CGGCTTCGCG CTGTTCTCGA TGCGCTCGCC GACGGTCACG CGGCTGTACC TGCAATGCAA GCCGGATGAA GACCTCGCCG AATGGTCCGA CGCGCGGATC TGGGACGAAC TGCACACGCG CTTCTCGAAC GACACCGGCT GGACGCCGAC CGAGGGCCGG ATCACGCAGA AGAGCGTGAC GCCGATGCGC AGCTTCGTGT CGGAGACGAT GCAGCACGGC CGCCTGTTCC TCGCCGGCGA CGCCGCGCAC ATCGTGCCGC CGACCGGCGC GAAGGGCATG AACCTCGCGG TGGCCGACGT CCGCGCGCTG TCCCGCGCGC TCGGCGCACG CTACCGGACG GGCGACGCGA CGCCGCTCGA GCGCTATTCG GCGACTTGCC TCGAACGCGT GTGGCGCGCC GAGCACTTCT CGTACTTCAT GACGAACATG CTGCATGCGT CGCCGGACGA TTCGCCGTTC GTCAATCGCC TGAAGTTCGC CGAGTTGAAA TACGTGACGC GCTCGCGGGC CGCCGCGCAG TCGCTCGCTG AAAACTACGT CGGCTTGCCG TTCGACGATG CGACGGCCTC CGAGGCAACC CGCCTTGACA ACGCGCTGTG CGCGACTCTA
Details for BuceA.00065.a.B1.GE36644
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 25
PERCENT COVERAGE: 83
Validated AA Sequence
MQHVRHEVRE VLGAPHAFEA SRRIALERRR VARPVACAER AGQRADVGHR EVHALRAGRR HDVRGVAGEE QAAVLHRLRH EAAHRRHALL RDPALGRRPA GVVREARVQF VPDPRVGPFG EVFIRLALQV QPRDRRRAHR EQREAVVVVC VHEFVERRRR VGEDAEPRER VDALERVQPV GRNRLPRDAV EAVAARDVVA VDAVGATVRM LERDERPLAF DIVQRDVADF EVHLMTVLDA GRDQVAYDFV LAVDGDRAPT GQLGQVDAVT LAVEQQLDAV MFEPFATHPV AEAGFVQRVD RALLEHAGAD AVLAVFARTR FDEDGIDALQ AQQVGKQKAG RTGADDRD
Validated NT Sequence
caaggcgggt tgcctcggag gccgtcgcat cgtcgaacgg caagccgacg tagttttcag cgagcgactg cgcggcggcc cgcgagcgcg tcacgtattt caactcggcg aacttcaggc gattgacgaa cggcgaatcg tccggcgacg catgcagcat gttcgtcatg aagtacgaga agtgctcggc gcgccacacg cgttcgaggc aagtcgccga atagcgctcg agcggcgtcg cgtcgcccgt ccggtagcgt gcgccgagcg cgcgggacag cgcgcggacg tcggccaccg cgaggttcat gcccttcgcg ccggtcggcg gcacgatgtg cgcggcgtcg ccggcgagga acaggcggcc gtgctgcatc gtctccgaca cgaagctgcg catcggcgtc acgctcttct gcgtgatccg gccctcggtc ggcgtccagc cggtgtcgtt cgagaagcgc gtgtgcagtt cgtcccagat ccgcgcgtcg gaccattcgg cgaggtcttc atccggcttg cattgcaggt acagccgcgt gaccgtcggc gagcgcatcg agaacagcgc gaagccgttg tcgtggtgtg cgtacacgag ttcgtcgagc gacggcgccg cgtcggcgag gatgccgagc cacgcgaacg ggtagacgcg ctcgaacgtg ttcagccggt cggccggaat cgtctgccgc gcgatgccgt ggaagccgtc gcagcccgcg atgtagtcgc agtcgatgcg gtcggcgcga ccgtccgcat gcttgaacgt gacgaacggc cgctcgcttt cgacatcgtg cagcgcgacg tcgctgactt cgaagtgcat ctgatgaccg tgctcgacgc cggccgcgat caggtcgcgt acgacttcgt gctggctgta gacggtgatc gcgcgcccac cggtcagctc ggacaggtcg atgcggtgac gctggccgtc gaacagcagc tcgatgccgt gatgttcgag cccttcgcga cgcatccggt cgccgaggcc ggcttcgttc agcgtgtcga ccgtgccctg ctcgagcacg ccggcgcgga tgcggttctc gcagtattcg cgcgaacgcg cttcgacgag gatggaatcg acgccttgca ggcgcagcag gtgggaaagc agaaggccgg acggaccggc gccgatgatc gcgacttg
Expected Protein Sequence
MAHHHHHHMR TQVAIIGAGP SGLLLSHLLR LQGVDSILVE ARSREYCENR IRAGVLEQGT VDTLNEAGLG DRMRREGLEH HGIELLFDGQ RHRIDLSELT GGRAITVYSQ HEVVRDLIAA GVEHGHQMHF EVSDVALHDV ESERPFVTFK HADGRADRID CDYIAGCDGF HGIARQTIPA DRLNTFERVY PFAWLGILAD AAPSLDELVY AHHDNGFALF SMRSPTVTRL YLQCKPDEDL AEWSDARIWD ELHTRFSNDT GWTPTEGRIT QKSVTPMRSF VSETMQHGRL FLAGDAAHIV PPTGAKGMNL AVADVRALSR ALGARYRTGD ATPLERYSAT CLERVWRAEH FSYFMTNMLH ASPDDSPFVN RLKFAELKYV TRSRAAAQSL AENYVGLPFD DATASEATRL DNALCATL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgca cccaagtcgc gatcatcggc gccggtccgt ccggccttct gctttcccac ctgctgcgcc tgcaaggcgt cgattccatc ctcgtcgaag cgcgttcgcg cgaatactgc gagaaccgca tccgcgccgg cgtgctcgag cagggcacgg tcgacacgct gaacgaagcc ggcctcggcg accggatgcg tcgcgaaggg ctcgaacatc acggcatcga gctgctgttc gacggccagc gtcaccgcat cgacctgtcc gagctgaccg gtgggcgcgc gatcaccgtc tacagccagc acgaagtcgt acgcgacctg atcgcggccg gcgtcgagca cggtcatcag atgcacttcg aagtcagcga cgtcgcgctg cacgatgtcg aaagcgagcg gccgttcgtc acgttcaagc atgcggacgg tcgcgccgac cgcatcgact gcgactacat cgcgggctgc gacggcttcc acggcatcgc gcggcagacg attccggccg accggctgaa cacgttcgag cgcgtctacc cgttcgcgtg gctcggcatc ctcgccgacg cggcgccgtc gctcgacgaa ctcgtgtacg cacaccacga caacggcttc gcgctgttct cgatgcgctc gccgacggtc acgcggctgt acctgcaatg caagccggat gaagacctcg ccgaatggtc cgacgcgcgg atctgggacg aactgcacac gcgcttctcg aacgacaccg gctggacgcc gaccgagggc cggatcacgc agaagagcgt gacgccgatg cgcagcttcg tgtcggagac gatgcagcac ggccgcctgt tcctcgccgg cgacgccgcg cacatcgtgc cgccgaccgg cgcgaagggc atgaacctcg cggtggccga cgtccgcgcg ctgtcccgcg cgctcggcgc acgctaccgg acgggcgacg cgacgccgct cgagcgctat tcggcgactt gcctcgaacg cgtgtggcgc gccgagcact tctcgtactt catgacgaac atgctgcatg cgtcgccgga cgattcgccg ttcgtcaatc gcctgaagtt cgccgagttg aaatacgtga cgcgctcgcg ggccgccgcg cagtcgctcg ctgaaaacta cgtcggcttg ccgttcgacg atgcgacggc ctccgaggca acccgccttg acaacgcgct gtgcgcgact ctatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat