BuceA.00063.a

ATP phosphoribosyltransferase regulatory subunit

CENTER ID: BuceA.00063.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00063.a.A1.GE33189 Full length( BuceA.00063.a ) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00063.a.A1.PS01282 Full length( BuceA.00063.a ) 1 382

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1947
RefSeq: YP_002231001.1
UniProt: B4EAH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTWLLPENI ADVLPSEARK IEELRRRLLD RFRSYGYEMV MPPLLEYLES LLTSGGADLR LRTFKLVDQL SGRTLGLRAD ITPQVARIDA HLLNRQGVTR LCYAGHVMHT RPRGLHATRE QIQIGAEIYG HAGLEADLEI QQLMLDALHL AGLSRIRLDL GHAGVLAALL ARDAQAAERG ESLYDALSGK DVPLLNELTD DLGADTRAAL RALPNLYGDA SVLAEARTRL PVLPEITRAL DDLAQLASQP KGVEVAIDLA DLRGYAYHSG AMFSAYIDGV PNAIARGGRY DHVGQAYGRA RPATGFSLDL RELARISPVE ARGTAILAPW AQDDALGAAV AALRDAGEVV IQALPGHDHV LDEFACDRSL VERNGAWVVE PR
NT Sequence
ATGTCGACCT GGTTACTTCC CGAGAATATC GCCGACGTGC TGCCGTCGGA AGCGCGCAAG ATCGAGGAGC TGCGCCGCAG GCTGCTCGAC CGCTTCCGTT CGTACGGCTA CGAAATGGTG ATGCCGCCGC TGCTCGAGTA TCTCGAGTCG CTGCTGACGA GCGGGGGCGC CGATCTGCGC CTGCGCACCT TCAAGCTGGT CGACCAGTTG TCGGGCCGGA CGCTCGGCCT GCGCGCGGAC ATCACGCCGC AGGTCGCGCG GATCGACGCG CACCTGTTGA ACCGTCAGGG CGTGACGCGC CTCTGCTATG CGGGCCACGT GATGCATACG CGTCCGCGCG GGCTGCACGC GACGCGCGAA CAGATCCAGA TCGGCGCGGA AATCTACGGC CACGCCGGGC TGGAAGCCGA TCTCGAGATC CAGCAGCTGA TGCTCGACGC GCTGCATCTC GCGGGCCTGT CGCGGATCCG CCTCGACCTC GGTCACGCGG GCGTGCTCGC GGCGCTGCTG GCGCGCGACG CGCAGGCCGC GGAGCGCGGC GAGTCGCTGT ACGACGCGCT GTCGGGCAAG GACGTGCCGC TGCTGAACGA GCTGACCGAC GATCTCGGCG CCGATACGCG CGCCGCGCTG CGTGCGCTGC CGAACCTTTA CGGCGACGCG AGCGTGCTCG CCGAGGCGCG CACGCGCCTG CCGGTGCTGC CCGAAATCAC GCGGGCGCTC GACGATCTCG CGCAGCTTGC GTCGCAGCCG AAGGGCGTCG AAGTCGCGAT CGATCTCGCC GATTTGCGCG GTTACGCGTA TCACAGCGGC GCGATGTTCA GCGCCTACAT CGACGGCGTG CCGAACGCGA TCGCGCGCGG CGGTCGTTAC GACCACGTGG GGCAGGCCTA TGGCCGGGCC CGCCCGGCAA CCGGCTTTTC GCTCGACCTG CGCGAACTCG CGCGGATTTC GCCGGTCGAG GCGCGCGGCA CCGCGATTCT CGCGCCGTGG GCGCAGGACG ACGCGCTCGG CGCGGCGGTC GCGGCGCTGC GCGATGCGGG CGAGGTCGTG ATCCAGGCGC TGCCGGGCCA CGACCACGTG CTCGACGAAT TCGCCTGCGA TCGTTCGCTC GTCGAGCGCA ACGGCGCATG GGTGGTCGAA CCCCGA
Details for BuceA.00063.a.A1.GE33189
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 43kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MSTWLLPENI ADVLPSEARK IEELRRRLLD RFRSYGYEMV MPPLLEYLES LLTSGGADLR LRTFKLVDQL SGRTLGLRAD ITPQVARIDA HLLNRQGVTR LCYAGHVMHT RPRGLHATRE QIQIGAEIYG HAGLEADLEI QQLMLDALHL AGLSRIRLDL GHAGVLAALL ARDAQAAERG ESLYDALSGK DVPLLNELTD DLGADTRAAL RALPNLYGDA SVLAEARTRL PVLPEITRAL DDLAQLASQP KGVEVAIDLA DLRGYAYHSG AMFSAYIDGV PNAIARGGRY DHVGQAYGRA RPATGFSLDL RELARISPVE ARGTAILAPW AQDDALGAAV AALRDAGEVV IQALPGHDHV LDEFACDRSL VERNGAWVVE PR
Validated NT Sequence
ttatcggggt tcgaccaccc atgcgccgtt gcgctcgacg agcgaacgat cgcaggcgaa ttcgtcgagc acgtggtcgt ggcccggcag cgcctggatc acgacctcgc ccgcatcgcg cagcgccgcg accgccgcgc cgagcgcgtc gtcctgcgcc cacggcgcga gaatcgcggt gccgcgcgcc tcgaccggcg aaatccgcgc gagttcgcgc aggtcgagcg aaaagccggt tgccgggcgg gcccggccat aggcctgccc cacgtggtcg taacgaccgc cgcgcgcgat cgcgttcggc acgccgtcga tgtaggcgct gaacatcgcg ccgctgtgat acgcgtaacc gcgcaaatcg gcgagatcga tcgcgacttc gacgcccttc ggctgcgacg caagctgcgc gagatcgtcg agcgcccgcg tgatttcggg cagcaccggc aggcgcgtgc gcgcctcggc gagcacgctc gcgtcgccgt aaaggttcgg cagcgcacgc agcgcggcgc gcgtatcggc gccgagatcg tcggtcagct cgttcagcag cggcacgtcc ttgcccgaca gcgcgtcgta cagcgactcg ccgcgctccg cggcctgcgc gtcgcgcgcc agcagcgccg cgagcacgcc cgcgtgaccg aggtcgaggc ggatccgcga caggcccgcg agatgcagcg cgtcgagcat cagctgctgg atctcgagat cggcttccag cccggcgtgg ccgtagattt ccgcgccgat ctggatctgt tcgcgcgtcg cgtgcagccc gcgcggacgc gtatgcatca cgtggcccgc atagcagagg cgcgtcacgc cctgacggtt caacaggtgc gcgtcgatcc gcgcgacctg cggcgtgatg tccgcgcgca ggccgagcgt ccggcccgac aactggtcga ccagcttgaa ggtgcgcagg cgcagatcgg cgcccccgct cgtcagcagc gactcgagat actcgagcag cggcggcatc accatttcgt agccgtacga acggaagcgg tcgagcagcc tgcggcgcag ctcctcgatc ttgcgcgctt ccgacggcag cacgtcggcg atattctcgg gaagtaacca ggtcgacatc gaaccaggac cctgggtctg agct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTWLLPEN IADVLPSEAR KIEELRRRLL DRFRSYGYEM VMPPLLEYLE SLLTSGGADL RLRTFKLVDQ LSGRTLGLRA DITPQVARID AHLLNRQGVT RLCYAGHVMH TRPRGLHATR EQIQIGAEIY GHAGLEADLE IQQLMLDALH LAGLSRIRLD LGHAGVLAAL LARDAQAAER GESLYDALSG KDVPLLNELT DDLGADTRAA LRALPNLYGD ASVLAEARTR LPVLPEITRA LDDLAQLASQ PKGVEVAIDL ADLRGYAYHS GAMFSAYIDG VPNAIARGGR YDHVGQAYGR ARPATGFSLD LRELARISPV EARGTAILAP WAQDDALGAA VAALRDAGEV VIQALPGHDH VLDEFACDRS LVERNGAWVV EPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgacctgg ttacttcccg agaatatcgc cgacgtgctg ccgtcggaag cgcgcaagat cgaggagctg cgccgcaggc tgctcgaccg cttccgttcg tacggctacg aaatggtgat gccgccgctg ctcgagtatc tcgagtcgct gctgacgagc gggggcgccg atctgcgcct gcgcaccttc aagctggtcg accagttgtc gggccggacg ctcggcctgc gcgcggacat cacgccgcag gtcgcgcgga tcgacgcgca cctgttgaac cgtcagggcg tgacgcgcct ctgctatgcg ggccacgtga tgcatacgcg tccgcgcggg ctgcacgcga cgcgcgaaca gatccagatc ggcgcggaaa tctacggcca cgccgggctg gaagccgatc tcgagatcca gcagctgatg ctcgacgcgc tgcatctcgc gggcctgtcg cggatccgcc tcgacctcgg tcacgcgggc gtgctcgcgg cgctgctggc gcgcgacgcg caggccgcgg agcgcggcga gtcgctgtac gacgcgctgt cgggcaagga cgtgccgctg ctgaacgagc tgaccgacga tctcggcgcc gatacgcgcg ccgcgctgcg tgcgctgccg aacctttacg gcgacgcgag cgtgctcgcc gaggcgcgca cgcgcctgcc ggtgctgccc gaaatcacgc gggcgctcga cgatctcgcg cagcttgcgt cgcagccgaa gggcgtcgaa gtcgcgatcg atctcgccga tttgcgcggt tacgcgtatc acagcggcgc gatgttcagc gcctacatcg acggcgtgcc gaacgcgatc gcgcgcggcg gtcgttacga ccacgtgggg caggcctatg gccgggcccg cccggcaacc ggcttttcgc tcgacctgcg cgaactcgcg cggatttcgc cggtcgaggc gcgcggcacc gcgattctcg cgccgtgggc gcaggacgac gcgctcggcg cggcggtcgc ggcgctgcgc gatgcgggcg aggtcgtgat ccaggcgctg ccgggccacg accacgtgct cgacgaattc gcctgcgatc gttcgctcgt cgagcgcaac ggcgcatggg tggtcgaacc ccgataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BuceA.00063.a.A1.PS01282
PURIFICATION DATe: 12/1/2011
CONCENTRATION: 41.16mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MSTWLLPENI ADVLPSEARK IEELRRRLLD RFRSYGYEMV MPPLLEYLES LLTSGGADLR LRTFKLVDQL SGRTLGLRAD ITPQVARIDA HLLNRQGVTR LCYAGHVMHT RPRGLHATRE QIQIGAEIYG HAGLEADLEI QQLMLDALHL AGLSRIRLDL GHAGVLAALL ARDAQAAERG ESLYDALSGK DVPLLNELTD DLGADTRAAL RALPNLYGDA SVLAEARTRL PVLPEITRAL DDLAQLASQP KGVEVAIDLA DLRGYAYHSG AMFSAYIDGV PNAIARGGRY DHVGQAYGRA RPATGFSLDL RELARISPVE ARGTAILAPW AQDDALGAAV AALRDAGEVV IQALPGHDHV LDEFACDRSL VERNGAWVVE PR
Validated NT Sequence
ttatcggggt tcgaccaccc atgcgccgtt gcgctcgacg agcgaacgat cgcaggcgaa ttcgtcgagc acgtggtcgt ggcccggcag cgcctggatc acgacctcgc ccgcatcgcg cagcgccgcg accgccgcgc cgagcgcgtc gtcctgcgcc cacggcgcga gaatcgcggt gccgcgcgcc tcgaccggcg aaatccgcgc gagttcgcgc aggtcgagcg aaaagccggt tgccgggcgg gcccggccat aggcctgccc cacgtggtcg taacgaccgc cgcgcgcgat cgcgttcggc acgccgtcga tgtaggcgct gaacatcgcg ccgctgtgat acgcgtaacc gcgcaaatcg gcgagatcga tcgcgacttc gacgcccttc ggctgcgacg caagctgcgc gagatcgtcg agcgcccgcg tgatttcggg cagcaccggc aggcgcgtgc gcgcctcggc gagcacgctc gcgtcgccgt aaaggttcgg cagcgcacgc agcgcggcgc gcgtatcggc gccgagatcg tcggtcagct cgttcagcag cggcacgtcc ttgcccgaca gcgcgtcgta cagcgactcg ccgcgctccg cggcctgcgc gtcgcgcgcc agcagcgccg cgagcacgcc cgcgtgaccg aggtcgaggc ggatccgcga caggcccgcg agatgcagcg cgtcgagcat cagctgctgg atctcgagat cggcttccag cccggcgtgg ccgtagattt ccgcgccgat ctggatctgt tcgcgcgtcg cgtgcagccc gcgcggacgc gtatgcatca cgtggcccgc atagcagagg cgcgtcacgc cctgacggtt caacaggtgc gcgtcgatcc gcgcgacctg cggcgtgatg tccgcgcgca ggccgagcgt ccggcccgac aactggtcga ccagcttgaa ggtgcgcagg cgcagatcgg cgcccccgct cgtcagcagc gactcgagat actcgagcag cggcggcatc accatttcgt agccgtacga acggaagcgg tcgagcagcc tgcggcgcag ctcctcgatc ttgcgcgctt ccgacggcag cacgtcggcg atattctcgg gaagtaacca ggtcgacatc gaaccaggac cctgggtctg agct
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTWLLPEN IADVLPSEAR KIEELRRRLL DRFRSYGYEM VMPPLLEYLE SLLTSGGADL RLRTFKLVDQ LSGRTLGLRA DITPQVARID AHLLNRQGVT RLCYAGHVMH TRPRGLHATR EQIQIGAEIY GHAGLEADLE IQQLMLDALH LAGLSRIRLD LGHAGVLAAL LARDAQAAER GESLYDALSG KDVPLLNELT DDLGADTRAA LRALPNLYGD ASVLAEARTR LPVLPEITRA LDDLAQLASQ PKGVEVAIDL ADLRGYAYHS GAMFSAYIDG VPNAIARGGR YDHVGQAYGR ARPATGFSLD LRELARISPV EARGTAILAP WAQDDALGAA VAALRDAGEV VIQALPGHDH VLDEFACDRS LVERNGAWVV EPR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGACCTGG TTACTTCCCG AGAATATCGC CGACGTGCTG CCGTCGGAAG CGCGCAAGAT CGAGGAGCTG CGCCGCAGGC TGCTCGACCG CTTCCGTTCG TACGGCTACG AAATGGTGAT GCCGCCGCTG CTCGAGTATC TCGAGTCGCT GCTGACGAGC GGGGGCGCCG ATCTGCGCCT GCGCACCTTC AAGCTGGTCG ACCAGTTGTC GGGCCGGACG CTCGGCCTGC GCGCGGACAT CACGCCGCAG GTCGCGCGGA TCGACGCGCA CCTGTTGAAC CGTCAGGGCG TGACGCGCCT CTGCTATGCG GGCCACGTGA TGCATACGCG TCCGCGCGGG CTGCACGCGA CGCGCGAACA GATCCAGATC GGCGCGGAAA TCTACGGCCA CGCCGGGCTG GAAGCCGATC TCGAGATCCA GCAGCTGATG CTCGACGCGC TGCATCTCGC GGGCCTGTCG CGGATCCGCC TCGACCTCGG TCACGCGGGC GTGCTCGCGG CGCTGCTGGC GCGCGACGCG CAGGCCGCGG AGCGCGGCGA GTCGCTGTAC GACGCGCTGT CGGGCAAGGA CGTGCCGCTG CTGAACGAGC TGACCGACGA TCTCGGCGCC GATACGCGCG CCGCGCTGCG TGCGCTGCCG AACCTTTACG GCGACGCGAG CGTGCTCGCC GAGGCGCGCA CGCGCCTGCC GGTGCTGCCC GAAATCACGC GGGCGCTCGA CGATCTCGCG CAGCTTGCGT CGCAGCCGAA GGGCGTCGAA GTCGCGATCG ATCTCGCCGA TTTGCGCGGT TACGCGTATC ACAGCGGCGC GATGTTCAGC GCCTACATCG ACGGCGTGCC GAACGCGATC GCGCGCGGCG GTCGTTACGA CCACGTGGGG CAGGCCTATG GCCGGGCCCG CCCGGCAACC GGCTTTTCGC TCGACCTGCG CGAACTCGCG CGGATTTCGC CGGTCGAGGC GCGCGGCACC GCGATTCTCG CGCCGTGGGC GCAGGACGAC GCGCTCGGCG CGGCGGTCGC GGCGCTGCGC GATGCGGGCG AGGTCGTGAT CCAGGCGCTG CCGGGCCACG ACCACGTGCT CGACGAATTC GCCTGCGATC GTTCGCTCGT CGAGCGCAAC GGCGCATGGG TGGTCGAACC CCGATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA