BuceA.00061.a

UDP-N-acetylglucosamine 2-epimerase

CENTER ID: BuceA.00061.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00061.a.B1.GE36643 Full length( BuceA.00061.a ) 1 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1373
RefSeq: YP_002230458.1
UniProt: B4E5K8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKILLVFGT RPEAIKMAPL VRALKAQADV DARVCVTAQH REMLDQVLTL FDIKPDYDLN LMRQSQTLTD VTTGILQAIG MVFDELRPDV VLVHGDTTTT LAVSLAAFYR YLPVGHVEAG LRSGDIWSPW PEELNRRVTD AVSSWHFAPT GQARDNLLSE GVPGGAVSLT GNTVIDALHE VKRMLDRTAA LSDKIAAYFP FLEPSRRVVL ITGHRRESFG EPFRHFCDAL CTLANRYRDA QFVYPLHLNP NVREPARARL GDVPNIYLIE PQEYLSFVFL MSRAYFIITD SGGIQEEGPA LGKPVLVTRE TTERPEAIQA GTARLVGTDQ DRIVWEASRL FDSESAYEEM SRASNPYGDG HASERIVHAL MRTPGVPTKT TSFSMGAAEM PFNALTLGLQ ALRSP
NT Sequence
ATGAAGAAGA TCTTGCTTGT GTTCGGGACC CGGCCGGAGG CCATCAAGAT GGCGCCGCTG GTGCGCGCAT TGAAGGCGCA AGCGGATGTC GACGCCCGCG TATGCGTGAC CGCGCAGCAT CGCGAAATGC TCGACCAGGT GCTAACGCTG TTCGACATCA AGCCCGACTA CGATCTCAAC CTGATGCGGC AAAGCCAGAC GCTGACCGAC GTGACGACCG GCATTCTCCA GGCGATCGGC ATGGTCTTCG ACGAACTGCG GCCGGACGTC GTGCTGGTGC ACGGCGATAC GACGACCACG CTCGCGGTCA GCCTCGCGGC GTTCTATCGC TATCTGCCGG TCGGGCACGT GGAGGCCGGC TTGCGCAGCG GCGACATCTG GTCGCCGTGG CCGGAGGAAC TGAATCGCCG CGTGACGGAT GCGGTGTCGT CCTGGCATTT CGCCCCGACC GGGCAGGCGC GCGACAACCT GCTGAGCGAA GGCGTGCCGG GCGGCGCGGT CTCGCTGACC GGCAATACCG TGATCGATGC GTTGCACGAG GTCAAGCGCA TGCTGGATCG CACCGCCGCG CTGTCGGACA AGATCGCCGC GTACTTCCCG TTTCTCGAAC CGTCGCGACG CGTCGTGCTG ATCACCGGCC ATCGCCGCGA AAGTTTCGGC GAACCGTTCC GGCATTTCTG CGACGCCCTG TGCACGCTGG CGAACCGCTA TCGCGATGCG CAGTTCGTCT ATCCGCTGCA CCTGAATCCG AACGTGAGGG AACCGGCGCG CGCGCGGCTC GGCGACGTCC CCAATATCTA CCTGATCGAG CCACAGGAAT ATCTTTCGTT CGTCTTCCTG ATGTCGCGCG CCTATTTCAT CATCACGGAT TCGGGCGGCA TTCAGGAAGA AGGGCCGGCG CTCGGCAAGC CGGTGCTGGT CACGCGCGAG ACGACCGAGC GGCCCGAGGC GATTCAGGCC GGCACCGCGC GGCTCGTCGG CACCGATCAG GACAGGATCG TATGGGAGGC GTCGCGTTTG TTCGACAGCG AGAGCGCTTA CGAGGAAATG TCGCGCGCGA GCAATCCGTA CGGTGACGGC CATGCGAGCG AGCGGATCGT TCACGCGCTG ATGCGCACGC CCGGCGTGCC GACGAAGACG ACGAGTTTCT CGATGGGCGC TGCGGAAATG CCGTTCAACG CGCTCACGCT CGGGTTGCAG GCATTGCGGT CGCCG
Details for BuceA.00061.a.B1.GE36643
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 46kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMK KILLVFGTRP EAIKMAPLVR ALKAQADVDA RVCVTAQHRE MLDQVLTLFD IKPDYDLNLM RQSQTLTDVT TGILQAIGMV FDELRPDVVL VHGDTTTTLA VSLAAFYRYL PVGHVEAGLR SGDIWSPWPE ELNRRVTDAV SSWHFAPTGQ ARDNLLSEGV PGGAVSLTGN TVIDALHEVK RMLDRTAALS DKIAAYFPFL EPSRRVVLIT GHRRESFGEP FRHFCDALCT LANRYRDAQF VYPLHLNPNV REPARARLGD VPNIYLIEPQ EYLSFVFLMS RAYFIITDXG GIXXEXXALG KXVLVTRETT XRXEAIXXXX XXXXXXXDXI VWXXXXXSXX XXXNVXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
attttgnnnn nnttaagaag gaganatacc atggctcacc accaccacca ccatatgaag aagatcttgc ttgtgttcgg gacccggccg gaggccatca agatggcgcc gctggtgcgc gcattgaagg cgcaagcgga tgtcgacgcc cgcgtatgcg tgaccgcgca gcatcgcgaa atgctcgacc aggtgctaac gctgttcgac atcaagcccg actacgatct caacctgatg cggcaaagcc agacgctgac cgacgtgacg accggcattc tccaggcgat cggcatggtc ttcgacgaac tgcggccgga cgtcgtgctg gtgcacggcg atacgacgac cacgctcgcg gtcagcctcg cggcgttcta tcgctatctg ccggtcgggc acgtggaggc cggcttgcgc agcggcgaca tctggtcgcc gtggccggag gaactgaatc gccgcgtgac ggatgcggtg tcgtcctggc atttcgcccc gaccgggcag gcgcgcgaca acctgctgag cgaaggcgtg ccgggcggcg cggtctcgct gaccggcaat accgtgatcg atgcgttgca cgaggtcaag cgcatgctgg atcgcaccgc cgcgctgtcg gacaagatcg ccgcgtactt cccgtttctc gaaccgtcgc gacgcgtcgt gctgatcacc ggccatcgcc gcgaaagttt cggcgaaccg ttccggcatt tctgcgacgc cctgtgcacg ctggcgaacc gctatcgcga tgcgcagttc gtctatccgc tgcacctgaa tccgaacgtg agggaaccgg cgcgcgcgcg gctcggcgac gtccccaata tctacctgat cgagccacag gaatatcttt cgttcgtctt cctgatgtcg cgcgcctatt tcatcatcac ggattnnggc ggcattcnnn aagaannnnn ggcgctcggc aagcnngtgc tggtcacgcg cgagacgacc gancgnnccg aggcgattcn nnnnncncnn nnnnnnnnnn ncnncnatcn ggacnngatc gtatggnnnn nnnnnnttnn ntcgannnnn ntnnnnnnna atgtnnnnnn nnnnnnannc nnnngnnnnn nnnntnnnnn nnnnnnnnnn ntnnnnnnnt nnntnnnnnn ncnnnnnnnn ncna
Expected Protein Sequence
MAHHHHHHMK KILLVFGTRP EAIKMAPLVR ALKAQADVDA RVCVTAQHRE MLDQVLTLFD IKPDYDLNLM RQSQTLTDVT TGILQAIGMV FDELRPDVVL VHGDTTTTLA VSLAAFYRYL PVGHVEAGLR SGDIWSPWPE ELNRRVTDAV SSWHFAPTGQ ARDNLLSEGV PGGAVSLTGN TVIDALHEVK RMLDRTAALS DKIAAYFPFL EPSRRVVLIT GHRRESFGEP FRHFCDALCT LANRYRDAQF VYPLHLNPNV REPARARLGD VPNIYLIEPQ EYLSFVFLMS RAYFIITDSG GIQEEGPALG KPVLVTRETT ERPEAIQAGT ARLVGTDQDR IVWEASRLFD SESAYEEMSR ASNPYGDGHA SERIVHALMR TPGVPTKTTS FSMGAAEMPF NALTLGLQAL RSP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga agatcttgct tgtgttcggg acccggccgg aggccatcaa gatggcgccg ctggtgcgcg cattgaaggc gcaagcggat gtcgacgccc gcgtatgcgt gaccgcgcag catcgcgaaa tgctcgacca ggtgctaacg ctgttcgaca tcaagcccga ctacgatctc aacctgatgc ggcaaagcca gacgctgacc gacgtgacga ccggcattct ccaggcgatc ggcatggtct tcgacgaact gcggccggac gtcgtgctgg tgcacggcga tacgacgacc acgctcgcgg tcagcctcgc ggcgttctat cgctatctgc cggtcgggca cgtggaggcc ggcttgcgca gcggcgacat ctggtcgccg tggccggagg aactgaatcg ccgcgtgacg gatgcggtgt cgtcctggca tttcgccccg accgggcagg cgcgcgacaa cctgctgagc gaaggcgtgc cgggcggcgc ggtctcgctg accggcaata ccgtgatcga tgcgttgcac gaggtcaagc gcatgctgga tcgcaccgcc gcgctgtcgg acaagatcgc cgcgtacttc ccgtttctcg aaccgtcgcg acgcgtcgtg ctgatcaccg gccatcgccg cgaaagtttc ggcgaaccgt tccggcattt ctgcgacgcc ctgtgcacgc tggcgaaccg ctatcgcgat gcgcagttcg tctatccgct gcacctgaat ccgaacgtga gggaaccggc gcgcgcgcgg ctcggcgacg tccccaatat ctacctgatc gagccacagg aatatctttc gttcgtcttc ctgatgtcgc gcgcctattt catcatcacg gattcgggcg gcattcagga agaagggccg gcgctcggca agccggtgct ggtcacgcgc gagacgaccg agcggcccga ggcgattcag gccggcaccg cgcggctcgt cggcaccgat caggacagga tcgtatggga ggcgtcgcgt ttgttcgaca gcgagagcgc ttacgaggaa atgtcgcgcg cgagcaatcc gtacggtgac ggccatgcga gcgagcggat cgttcacgcg ctgatgcgca cgcccggcgt gccgacgaag acgacgagtt tctcgatggg cgctgcggaa atgccgttca acgcgctcac gctcgggttg caggcattgc ggtcgccgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat