BuceA.00058.a

Alkyl hydroperoxide reductase subunit F (EC 1.6.4.-)

CENTER ID: BuceA.00058.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00058.a.B1.GE36620 full length 1 533
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00058.a.B1.PS01821 full length 1 533

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4945
RefSeq: YP_002233832.1
UniProt: B4EH96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLDANLKNQL KAYLEKITRP IELVASLDDS AKSQELLALL NEIASLTDRV TVTERRDDAE RKPSFSIGEP GKPAGIRFAG IPMGHEFTSL VLALLQTGGH PIKLDDDVIE QIRALDGDYA FETFFSLSCQ NCPEVVQALN VMSLINPRIR HVAIDGALFQ DEVESRQIMA VPTMFLNGAS FGQGRSSVKE ILAKLDTGAG ERAAKSLENK PVFDTLIVGG GPAGAAAAIY SARKGIATGV VAERFGGQVL DTMAIENFVS VQETEGPKFA TALEQHVKQY DVDIMDVQRA DALIPGDVHQ IRLANGAVLK AKTIVLATGA RWREINVPGE REYRNRGVAY CPHCDGPLFK GKRVAVVGGG NSGVEAAIDL AGIVKEVTLI EYGAQLRADE VLQRKLRSLP NVTIVTQAQT TELTGDGSKL NGLVYKDLRS GETKRVDLEG VFVQIGLVPN TEWLKGTVEL SKHGEIVVDA RGATSVPGVF AAGDVTTVPF KQIVIAVGEG AKASLGAFDH LIRQDVAAPA ATAQPRVEEA VAA
NT Sequence
atgctcgacg ccaatctcaa gaaccaactc aaagcgtacc tcgaaaaaat cacgcgcccg atcgaactcg tcgcctcgct cgacgacagc gcgaaatcgc aggaactgct ggcgctgctg aacgagattg cgtcgctgac ggatcgcgtg accgtgaccg aacgccgcga cgacgccgag cgcaagccgt cgttctcgat cggcgagccc ggcaagccgg ccggcatccg tttcgccggc atcccgatgg gccatgaatt cacgtcgctc gtgctcgcgc tgctgcagac gggcggccat ccgatcaagc tcgacgacga cgtgatcgaa cagattcgcg cgctcgacgg cgactacgca ttcgaaacgt ttttctcgct gtcgtgccag aactgcccgg aagtcgtgca ggcgctgaac gtgatgtcgc tgatcaaccc gcgcatccgc cacgtcgcga tcgacggcgc actgttccag gatgaagtcg agtcgcgcca gatcatggcc gtgccgacga tgttcctgaa cggcgcgtcg ttcggccagg gccgcagcag cgtgaaggaa atcctcgcga agctcgatac gggcgccgga gaacgcgcgg cgaagtcgct cgaaaacaag ccggtgttcg acacgctgat cgtcggcggc ggcccggcgg gcgcggctgc tgcgatctac tccgcacgca agggcatcgc gacgggtgtc gtcgccgagc gtttcggcgg ccaggtgctg gacacgatgg cgatcgagaa cttcgtgtcg gtgcaggaaa ccgaagggcc gaagttcgcg accgcactcg agcagcacgt caagcagtac gacgtcgaca tcatggacgt gcaacgcgcc gacgcgctga tcccgggcga cgtgcaccag atccgtctcg cgaacggcgc ggtgctgaaa gcgaagacga tcgtcctcgc gaccggcgca cgctggcgtg aaatcaacgt gccgggcgag cgcgaatacc gcaaccgcgg ggtcgcgtac tgcccgcact gcgacggccc gctgttcaag ggcaagcggg tcgcggtggt cggcggcggc aactcgggcg tcgaggccgc gatcgacctc gccggcatcg tgaaggaagt cacgctgatc gaatacggcg cgcaactgcg cgcggacgaa gtgctgcaac gcaagctgcg cagcctgccg aacgtgacga tcgtcacgca agcgcagacg accgaactga ccggcgacgg cagcaagctg aacgggctcg tctacaagga tctgcgttcc ggtgaaacga agcgcgtcga cctcgaaggc gtgttcgtgc agatcggcct cgtgccgaac accgaatggc tgaaaggcac ggtcgaactg tcgaagcacg gcgagatcgt cgtcgatgcg cgcggcgcga cgtcggtgcc gggcgtgttc gcggccggtg acgtgacgac ggtgccgttc aagcagatcg tgatcgcggt cggcgaaggg gcgaaggcgt cgctcggcgc gttcgaccac ctgatccggc aggacgtggc ggcgccggcc gccaccgcgc agccgcgagt cgaagaagcg gtcgccgcct ga
Details for BuceA.00058.a.B1.GE36620
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 81
Validated AA Sequence
MGHEFTSLVL ALLQTGGHPI KLDDDVIEQI RALDGDYAFE TFFSLSCQNC PEVVQALNVM SLINPRIRHV AIDGALFQDE VESRQIMAVP TMFLNGASFG QGRSSVKEIL AKLDTGAGER AAKSLENKPV FDTLIVGGGP AGAAAAIYSA RKGIATGVVA ERFGGQVLDT MAIENFVSVQ ETEGPKFATA LEQHVKQYDV DIMDVQRADA LIPGDVHQIR LANGAVLKAK TIVLATGARW REINVPXERE YRNRGVAYCP HCDGPLFKGK RVAVVGGGNS GVEAAXDLAG IVKEVTXIEY GXQLRADEVL QRKXRSXPNV TIVTQAQTTE LTGDGSKLNG LVYKDLRSGE TKRVDLEGVF VQIGLVPNTE WLKGTVELSK HGEIVVDARG ATSVPGVFAA GDVTTVPFKQ IVIAVGEGAK ASLGAFDHLI RQDVAAPAAT AQP
Validated NT Sequence
gcggctgcgc ggtggcggcc ggcgccgcca cgtcctgccg gatcaggtgg tcgaacgcgc cgagcgacgc cttcgcccct tcgccgaccg cgatcacgat ctgcttgaac ggcaccgtcg tcacgtcacc ggccgcgaac acgcccggca ccgacgtcgc gccgcgcgca tcgacgacga tctcgccgtg cttcgacagt tcgaccgtgc ctttcagcca ttcggtgttc ggcacgaggc cgatctgcac gaacacgcct tcgaggtcna cgcgcttcgt ttcaccggaa cgcagatcct tgtagacgag cccgttcagc ttgctgccgt cgccggtcag ttcggtcgtc tgcgcttgcg tgacgatcgt cacgttcggn angctgcgca ncttgcgttg cagcacttcg tccgcgcgca gttgcncgcc gtattcgatc ancgtgactt ccttcacgat gccggcgagg tcnatcgcgg cctcgacgcc cgagttgccg ccgccnacca ccgcgacccg cttgcccttg aacagcgggc cgtcgcagtg cgggcagtac gcgaccccgc ggttgcggta ttcgcgctcg cncggnacgt tgatttcacg ccagcgtgcg ccggtcgcga ggacgatcgt cttcgctttc agcaccgcgc cgttcgcgag acggatctgg tgcacgtcgc ccgggatcag cgcgtcggcg cgttgcacgt ccatgatgtc gacgtcgtac tgcttgacgt gctgctcgag tgcggtcgcg aacttcggcc cttcggtttc ctgcaccgac acgaagttct cgatcgccat cgtgtccagc acctggccgc cgaaacgctc ggcgacgaca ccngtcgcga tgcccttgcg tgcggagtag atcgcagcag ccgcgcccgc cgggccgccg ccgacgatca gcgtgtcgaa caccggcttg ttttcgagcg acttcgccgc gcgttctccg gcgcccgtat cgagcttcgc gaggatttcc ttcacgctgc tgcggccctg gccgaacgac gcgccgttca ggaacatcgt cggcacggcc atgatctggc gcgactcgac ttcatcctgg aacagtgcgc cgtcgatcgc gacgtggcgg atgcgcgggt tgatcagcga catcacgttc agcgcctgca cgacttccgg gcagttctgg cacgacagcg agaaaaacgt ttcgaatgcg tagtcgccgt cgagcgcgcg aatctgttcg atcacgtcgt cgtcgagctt gatcggatgg ccgcccgtct gcagcagcgc gagcacgagc gacgtgaatt catggcccat cgggatgccg gcgaaacgga tgccggccgg cttgccgggc tcgccgatcg agaacgacgg cttgcgctcg gcgtcgtcgc ggcgttcggt cacggtcacg cgatccgtca gcgacgcaat ctcgttcagc agcgccagca gttcctgcga tttcgcgctg tcgtcgagcg aggcgacgag ttcgatcggg cgcgtgattt tttcgaggta cgctttgagt tggttcttga gattggcgtc
Expected Protein Sequence
MAHHHHHHML DANLKNQLKA YLEKITRPIE LVASLDDSAK SQELLALLNE IASLTDRVTV TERRDDAERK PSFSIGEPGK PAGIRFAGIP MGHEFTSLVL ALLQTGGHPI KLDDDVIEQI RALDGDYAFE TFFSLSCQNC PEVVQALNVM SLINPRIRHV AIDGALFQDE VESRQIMAVP TMFLNGASFG QGRSSVKEIL AKLDTGAGER AAKSLENKPV FDTLIVGGGP AGAAAAIYSA RKGIATGVVA ERFGGQVLDT MAIENFVSVQ ETEGPKFATA LEQHVKQYDV DIMDVQRADA LIPGDVHQIR LANGAVLKAK TIVLATGARW REINVPGERE YRNRGVAYCP HCDGPLFKGK RVAVVGGGNS GVEAAIDLAG IVKEVTLIEY GAQLRADEVL QRKLRSLPNV TIVTQAQTTE LTGDGSKLNG LVYKDLRSGE TKRVDLEGVF VQIGLVPNTE WLKGTVELSK HGEIVVDARG ATSVPGVFAA GDVTTVPFKQ IVIAVGEGAK ASLGAFDHLI RQDVAAPAAT AQPRVEEAVA A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctcg acgccaatct caagaaccaa ctcaaagcgt acctcgaaaa aatcacgcgc ccgatcgaac tcgtcgcctc gctcgacgac agcgcgaaat cgcaggaact gctggcgctg ctgaacgaga ttgcgtcgct gacggatcgc gtgaccgtga ccgaacgccg cgacgacgcc gagcgcaagc cgtcgttctc gatcggcgag cccggcaagc cggccggcat ccgtttcgcc ggcatcccga tgggccatga attcacgtcg ctcgtgctcg cgctgctgca gacgggcggc catccgatca agctcgacga cgacgtgatc gaacagattc gcgcgctcga cggcgactac gcattcgaaa cgtttttctc gctgtcgtgc cagaactgcc cggaagtcgt gcaggcgctg aacgtgatgt cgctgatcaa cccgcgcatc cgccacgtcg cgatcgacgg cgcactgttc caggatgaag tcgagtcgcg ccagatcatg gccgtgccga cgatgttcct gaacggcgcg tcgttcggcc agggccgcag cagcgtgaag gaaatcctcg cgaagctcga tacgggcgcc ggagaacgcg cggcgaagtc gctcgaaaac aagccggtgt tcgacacgct gatcgtcggc ggcggcccgg cgggcgcggc tgctgcgatc tactccgcac gcaagggcat cgcgacgggt gtcgtcgccg agcgtttcgg cggccaggtg ctggacacga tggcgatcga gaacttcgtg tcggtgcagg aaaccgaagg gccgaagttc gcgaccgcac tcgagcagca cgtcaagcag tacgacgtcg acatcatgga cgtgcaacgc gccgacgcgc tgatcccggg cgacgtgcac cagatccgtc tcgcgaacgg cgcggtgctg aaagcgaaga cgatcgtcct cgcgaccggc gcacgctggc gtgaaatcaa cgtgccgggc gagcgcgaat accgcaaccg cggggtcgcg tactgcccgc actgcgacgg cccgctgttc aagggcaagc gggtcgcggt ggtcggcggc ggcaactcgg gcgtcgaggc cgcgatcgac ctcgccggca tcgtgaagga agtcacgctg atcgaatacg gcgcgcaact gcgcgcggac gaagtgctgc aacgcaagct gcgcagcctg ccgaacgtga cgatcgtcac gcaagcgcag acgaccgaac tgaccggcga cggcagcaag ctgaacgggc tcgtctacaa ggatctgcgt tccggtgaaa cgaagcgcgt cgacctcgaa ggcgtgttcg tgcagatcgg cctcgtgccg aacaccgaat ggctgaaagg cacggtcgaa ctgtcgaagc acggcgagat cgtcgtcgat gcgcgcggcg cgacgtcggt gccgggcgtg ttcgcggccg gtgacgtgac gacggtgccg ttcaagcaga tcgtgatcgc ggtcggcgaa ggggcgaagg cgtcgctcgg cgcgttcgac cacctgatcc ggcaggacgt ggcggcgccg gccgccaccg cgcagccgcg agtcgaagaa gcggtcgccg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuceA.00058.a.B1.PS01821
PURIFICATION DATe: 5/14/2013
CONCENTRATION: 50mg/ml
OBSERVED MW: 51kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 81
Protocol Notes
notes unavailable
Validated AA Sequence
MGHEFTSLVL ALLQTGGHPI KLDDDVIEQI RALDGDYAFE TFFSLSCQNC PEVVQALNVM SLINPRIRHV AIDGALFQDE VESRQIMAVP TMFLNGASFG QGRSSVKEIL AKLDTGAGER AAKSLENKPV FDTLIVGGGP AGAAAAIYSA RKGIATGVVA ERFGGQVLDT MAIENFVSVQ ETEGPKFATA LEQHVKQYDV DIMDVQRADA LIPGDVHQIR LANGAVLKAK TIVLATGARW REINVPXERE YRNRGVAYCP HCDGPLFKGK RVAVVGGGNS GVEAAXDLAG IVKEVTXIEY GXQLRADEVL QRKXRSXPNV TIVTQAQTTE LTGDGSKLNG LVYKDLRSGE TKRVDLEGVF VQIGLVPNTE WLKGTVELSK HGEIVVDARG ATSVPGVFAA GDVTTVPFKQ IVIAVGEGAK ASLGAFDHLI RQDVAAPAAT AQP
Validated NT Sequence
gcggctgcgc ggtggcggcc ggcgccgcca cgtcctgccg gatcaggtgg tcgaacgcgc cgagcgacgc cttcgcccct tcgccgaccg cgatcacgat ctgcttgaac ggcaccgtcg tcacgtcacc ggccgcgaac acgcccggca ccgacgtcgc gccgcgcgca tcgacgacga tctcgccgtg cttcgacagt tcgaccgtgc ctttcagcca ttcggtgttc ggcacgaggc cgatctgcac gaacacgcct tcgaggtcna cgcgcttcgt ttcaccggaa cgcagatcct tgtagacgag cccgttcagc ttgctgccgt cgccggtcag ttcggtcgtc tgcgcttgcg tgacgatcgt cacgttcggn angctgcgca ncttgcgttg cagcacttcg tccgcgcgca gttgcncgcc gtattcgatc ancgtgactt ccttcacgat gccggcgagg tcnatcgcgg cctcgacgcc cgagttgccg ccgccnacca ccgcgacccg cttgcccttg aacagcgggc cgtcgcagtg cgggcagtac gcgaccccgc ggttgcggta ttcgcgctcg cncggnacgt tgatttcacg ccagcgtgcg ccggtcgcga ggacgatcgt cttcgctttc agcaccgcgc cgttcgcgag acggatctgg tgcacgtcgc ccgggatcag cgcgtcggcg cgttgcacgt ccatgatgtc gacgtcgtac tgcttgacgt gctgctcgag tgcggtcgcg aacttcggcc cttcggtttc ctgcaccgac acgaagttct cgatcgccat cgtgtccagc acctggccgc cgaaacgctc ggcgacgaca ccngtcgcga tgcccttgcg tgcggagtag atcgcagcag ccgcgcccgc cgggccgccg ccgacgatca gcgtgtcgaa caccggcttg ttttcgagcg acttcgccgc gcgttctccg gcgcccgtat cgagcttcgc gaggatttcc ttcacgctgc tgcggccctg gccgaacgac gcgccgttca ggaacatcgt cggcacggcc atgatctggc gcgactcgac ttcatcctgg aacagtgcgc cgtcgatcgc gacgtggcgg atgcgcgggt tgatcagcga catcacgttc agcgcctgca cgacttccgg gcagttctgg cacgacagcg agaaaaacgt ttcgaatgcg tagtcgccgt cgagcgcgcg aatctgttcg atcacgtcgt cgtcgagctt gatcggatgg ccgcccgtct gcagcagcgc gagcacgagc gacgtgaatt catggcccat cgggatgccg gcgaaacgga tgccggccgg cttgccgggc tcgccgatcg agaacgacgg cttgcgctcg gcgtcgtcgc ggcgttcggt cacggtcacg cgatccgtca gcgacgcaat ctcgttcagc agcgccagca gttcctgcga tttcgcgctg tcgtcgagcg aggcgacgag ttcgatcggg cgcgtgattt tttcgaggta cgctttgagt tggttcttga gattggcgtc
Expressed Protein Sequence
MAHHHHHHML DANLKNQLKA YLEKITRPIE LVASLDDSAK SQELLALLNE IASLTDRVTV TERRDDAERK PSFSIGEPGK PAGIRFAGIP MGHEFTSLVL ALLQTGGHPI KLDDDVIEQI RALDGDYAFE TFFSLSCQNC PEVVQALNVM SLINPRIRHV AIDGALFQDE VESRQIMAVP TMFLNGASFG QGRSSVKEIL AKLDTGAGER AAKSLENKPV FDTLIVGGGP AGAAAAIYSA RKGIATGVVA ERFGGQVLDT MAIENFVSVQ ETEGPKFATA LEQHVKQYDV DIMDVQRADA LIPGDVHQIR LANGAVLKAK TIVLATGARW REINVPGERE YRNRGVAYCP HCDGPLFKGK RVAVVGGGNS GVEAAIDLAG IVKEVTLIEY GAQLRADEVL QRKLRSLPNV TIVTQAQTTE LTGDGSKLNG LVYKDLRSGE TKRVDLEGVF VQIGLVPNTE WLKGTVELSK HGEIVVDARG ATSVPGVFAA GDVTTVPFKQ IVIAVGEGAK ASLGAFDHLI RQDVAAPAAT AQPRVEEAVA A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTCG ACGCCAATCT CAAGAACCAA CTCAAAGCGT ACCTCGAAAA AATCACGCGC CCGATCGAAC TCGTCGCCTC GCTCGACGAC AGCGCGAAAT CGCAGGAACT GCTGGCGCTG CTGAACGAGA TTGCGTCGCT GACGGATCGC GTGACCGTGA CCGAACGCCG CGACGACGCC GAGCGCAAGC CGTCGTTCTC GATCGGCGAG CCCGGCAAGC CGGCCGGCAT CCGTTTCGCC GGCATCCCGA TGGGCCATGA ATTCACGTCG CTCGTGCTCG CGCTGCTGCA GACGGGCGGC CATCCGATCA AGCTCGACGA CGACGTGATC GAACAGATTC GCGCGCTCGA CGGCGACTAC GCATTCGAAA CGTTTTTCTC GCTGTCGTGC CAGAACTGCC CGGAAGTCGT GCAGGCGCTG AACGTGATGT CGCTGATCAA CCCGCGCATC CGCCACGTCG CGATCGACGG CGCACTGTTC CAGGATGAAG TCGAGTCGCG CCAGATCATG GCCGTGCCGA CGATGTTCCT GAACGGCGCG TCGTTCGGCC AGGGCCGCAG CAGCGTGAAG GAAATCCTCG CGAAGCTCGA TACGGGCGCC GGAGAACGCG CGGCGAAGTC GCTCGAAAAC AAGCCGGTGT TCGACACGCT GATCGTCGGC GGCGGCCCGG CGGGCGCGGC TGCTGCGATC TACTCCGCAC GCAAGGGCAT CGCGACGGGT GTCGTCGCCG AGCGTTTCGG CGGCCAGGTG CTGGACACGA TGGCGATCGA GAACTTCGTG TCGGTGCAGG AAACCGAAGG GCCGAAGTTC GCGACCGCAC TCGAGCAGCA CGTCAAGCAG TACGACGTCG ACATCATGGA CGTGCAACGC GCCGACGCGC TGATCCCGGG CGACGTGCAC CAGATCCGTC TCGCGAACGG CGCGGTGCTG AAAGCGAAGA CGATCGTCCT CGCGACCGGC GCACGCTGGC GTGAAATCAA CGTGCCGGGC GAGCGCGAAT ACCGCAACCG CGGGGTCGCG TACTGCCCGC ACTGCGACGG CCCGCTGTTC AAGGGCAAGC GGGTCGCGGT GGTCGGCGGC GGCAACTCGG GCGTCGAGGC CGCGATCGAC CTCGCCGGCA TCGTGAAGGA AGTCACGCTG ATCGAATACG GCGCGCAACT GCGCGCGGAC GAAGTGCTGC AACGCAAGCT GCGCAGCCTG CCGAACGTGA CGATCGTCAC GCAAGCGCAG ACGACCGAAC TGACCGGCGA CGGCAGCAAG CTGAACGGGC TCGTCTACAA GGATCTGCGT TCCGGTGAAA CGAAGCGCGT CGACCTCGAA GGCGTGTTCG TGCAGATCGG CCTCGTGCCG AACACCGAAT GGCTGAAAGG CACGGTCGAA CTGTCGAAGC ACGGCGAGAT CGTCGTCGAT GCGCGCGGCG CGACGTCGGT GCCGGGCGTG TTCGCGGCCG GTGACGTGAC GACGGTGCCG TTCAAGCAGA TCGTGATCGC GGTCGGCGAA GGGGCGAAGG CGTCGCTCGG CGCGTTCGAC CACCTGATCC GGCAGGACGT GGCGGCGCCG GCCGCCACCG CGCAGCCGCG AGTCGAAGAA GCGGTCGCCG CCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT