BuceA.00057.u

ABC transporter ATP-binding protein

CENTER ID: BuceA.00057.u
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00057.u.A1.GE33184 Full length( BuceA.00057.u ) 1 255
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6831
RefSeq: YP_002153536.1
UniProt: B4EPR0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEAILEARG IVKRYGKFTA LGGVDLRIMP RTVHSVIGPN GAGKTTLFHT LTGTLPITSG SILFDGHDVS HEPDHKRVRR GIARSFQVTS LFPNLSVREN LRVAAQGVES RRALNPWTPP RGALAHDGII DDVLERLGLQ RFADTASGVL SHGQQRRLEV GMALAARPRA IFLDEPTSGM GIDDLDDMKA LIRGLRDDYT VVLIEHNMGI VMDISDTITV MQQGRVLVEG RPDAIRGDAR VRSAYLGNMI TGGRA
NT Sequence
ATGAGCGAAG CGATTCTCGA AGCGCGCGGC ATCGTCAAGC GCTACGGCAA GTTCACCGCG CTGGGCGGCG TCGATCTGCG CATCATGCCG CGCACCGTGC ATTCGGTGAT CGGCCCGAAC GGCGCGGGCA AGACGACGCT GTTCCATACG CTGACCGGCA CGCTGCCGAT CACGTCGGGC TCGATCCTGT TCGACGGGCA CGACGTGTCG CACGAACCCG ATCACAAGCG CGTGCGGCGC GGCATCGCCC GCTCGTTCCA GGTGACGAGC CTGTTTCCGA ACCTGAGCGT GCGCGAAAAC CTGCGCGTCG CCGCGCAGGG CGTCGAATCG CGGCGGGCGC TCAATCCGTG GACGCCGCCG CGCGGGGCGC TCGCGCACGA CGGCATCATC GACGACGTAC TCGAGCGGCT CGGCCTGCAG CGCTTCGCCG ACACGGCGTC CGGCGTGCTG TCGCATGGGC AGCAGCGCCG GCTCGAGGTC GGCATGGCGC TGGCGGCGCG GCCGCGCGCG ATCTTTCTCG ACGAGCCGAC GTCGGGCATG GGCATCGACG ATCTCGACGA CATGAAGGCG CTGATCCGCG GCCTGCGCGA CGACTACACG GTCGTGCTGA TCGAACACAA CATGGGCATC GTGATGGACA TCTCGGACAC GATCACGGTG ATGCAGCAGG GACGCGTGCT GGTCGAAGGC CGACCCGATG CGATTCGCGG CGACGCGCGC GTGCGCAGTG CGTATCTCGG CAACATGATC ACGGGAGGCC GCGCA
Details for BuceA.00057.u.A1.GE33184
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 106
Validated AA Sequence
MSEAILEARG IVKRYGKFTA LGGVDLRIMP RTVHSVIGPN GAGKTTLFHT LTGTLPITSG SILFDGHDVS HEPDHKRVRR GIARSFQVTS LFPNLSVREN LRVAAQGVES RRALNPWTPP RGALAHDGII DDVLERLGLQ RFADTASGVL SHGQQRRLEV GMALAARPRA IFLDEPTSGM GIDDLDDMKA LIRGLRDDYT VVLIEHNMGI VMDISDTITV MQXGRVLVEG RPDAIRGDAR VRSAYLGNMI TGGRAXXKAR TSSAAKLLED PAANKARKEA XLAXXXXXXX XKK
Validated NT Sequence
ccannatatn gntaccctgg aagctcagac ccagggtcct ggttcgatga gcgaagcgat tctcgaagcg cgcggcatcg tcaagcgcta cggcaagttc accgcgctgg gcggcgtcga tctgcgcatc atgccgcgca ccgtgcattc ggtgatcggc ccgaacggcg cgggcaagac gacgctgttc catacgctga ccggcacgct gccgatcacg tcgggctcga tcctgttcga cgggcacgac gtgtcgcacg aacccgatca caagcgcgtg cggcgcggca tcgcccgctc gttccaggtg acgagcctgt ttccgaacct gagcgtgcgc gaaaacctgc gcgtcgccgc gcagggcgtc gaatcgcggc gggcgctcaa tccgtggacg ccgccgcgcg gggcgctcgc gcacgacggc atcatcgacg acgtactcga gcggctcggc ctgcagcgct tcgccgacac ggcgtccggc gtgctgtcgc atgggcagca gcgccggctc gaggtcggca tggcgctggc ggcgcggccg cgcgcgatct ttctcgacga gccgacgtcg ggcatgggca tcgacgatct cgacgacatg aaggcgctga tccgcggcct gcgcgacgac tacacggtcg tgctgatcga acacaacatg ggcatcgtga tggacatctc ggacacgatc acggtgatgc agcanggacg cgtgctggtc gaaggccgac ccgatgcgat tcgcggcgac gcgcgcgtgc gcagtgcgta tctcggcaac atgatcacgg gaggccgcgc nnnaanaaaa gcacgaacaa gttctgcagc caagcttctc gaggatccng ctgctaacaa agcccgaaag gaagctgant tggctnnnnn nnnnnnnnaa aannnnnnaa aaaaaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEAILEAR GIVKRYGKFT ALGGVDLRIM PRTVHSVIGP NGAGKTTLFH TLTGTLPITS GSILFDGHDV SHEPDHKRVR RGIARSFQVT SLFPNLSVRE NLRVAAQGVE SRRALNPWTP PRGALAHDGI IDDVLERLGL QRFADTASGV LSHGQQRRLE VGMALAARPR AIFLDEPTSG MGIDDLDDMK ALIRGLRDDY TVVLIEHNMG IVMDISDTIT VMQQGRVLVE GRPDAIRGDA RVRSAYLGNM ITGGRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgaagcg attctcgaag cgcgcggcat cgtcaagcgc tacggcaagt tcaccgcgct gggcggcgtc gatctgcgca tcatgccgcg caccgtgcat tcggtgatcg gcccgaacgg cgcgggcaag acgacgctgt tccatacgct gaccggcacg ctgccgatca cgtcgggctc gatcctgttc gacgggcacg acgtgtcgca cgaacccgat cacaagcgcg tgcggcgcgg catcgcccgc tcgttccagg tgacgagcct gtttccgaac ctgagcgtgc gcgaaaacct gcgcgtcgcc gcgcagggcg tcgaatcgcg gcgggcgctc aatccgtgga cgccgccgcg cggggcgctc gcgcacgacg gcatcatcga cgacgtactc gagcggctcg gcctgcagcg cttcgccgac acggcgtccg gcgtgctgtc gcatgggcag cagcgccggc tcgaggtcgg catggcgctg gcggcgcggc cgcgcgcgat ctttctcgac gagccgacgt cgggcatggg catcgacgat ctcgacgaca tgaaggcgct gatccgcggc ctgcgcgacg actacacggt cgtgctgatc gaacacaaca tgggcatcgt gatggacatc tcggacacga tcacggtgat gcagcaggga cgcgtgctgg tcgaaggccg acccgatgcg attcgcggcg acgcgcgcgt gcgcagtgcg tatctcggca acatgatcac gggaggccgc gcataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa