BuceA.00057.t

Putative sugar ABC transporter ATP-binding protein

CENTER ID: BuceA.00057.t
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00057.t.A1.GE33183 Full length( BuceA.00057.t ) 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6640
RefSeq: YP_002235406.1
UniProt: B4ELY4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASISMRRVQ KAYGEHAPVI RDVDLEIGAH EFCVFLGPSG CGKSTLLRMI AGLEDLSAGE LSIDGRRVDD VPAAERGVAM VFQSYALFPH MTVYENMAFG LKLARVPKAE IDRKVRDAAR ILQLDTLLER KPKALSGGQR QRVAIGRAIV REPGVFLFDE PLSNLDAALR GQTRIEIARL HRQFERASVV YVTHDQTEAM TLADKIVLLH AGADTAQHGS IAQVGAPLDL YHHPASRFVA GFIGSPRMNF LPAVVTACDA QRTTVKLVPS GETFALPRDG SALSAGAAVT LGVRPEHLTL GAAADATTLA RDVALVERLG EQTYVHLDSP GLPDATPLIA KVPGDAQVRT GERVHVHAPA AACHLFTEDG RAVPACADVH VHHYA
NT Sequence
ATGGCGAGCA TCTCGATGCG ACGCGTGCAG AAAGCCTATG GCGAGCACGC GCCGGTCATT CGCGACGTCG ATCTGGAGAT CGGCGCGCAC GAGTTCTGCG TGTTTCTCGG GCCGTCCGGA TGCGGGAAGT CGACCCTGTT GCGCATGATC GCCGGGCTCG AGGATCTGAG CGCGGGCGAG TTGTCGATCG ACGGCCGCCG CGTCGACGAC GTGCCGGCCG CCGAACGCGG CGTCGCGATG GTGTTCCAGA GCTACGCGCT GTTTCCGCAC ATGACGGTGT ACGAGAACAT GGCGTTCGGG CTGAAGCTCG CGCGCGTGCC GAAAGCCGAG ATCGACCGGA AGGTGCGCGA CGCCGCGCGC ATCCTGCAGC TCGACACGCT GCTCGAACGC AAGCCGAAGG CGCTGTCGGG CGGCCAGCGG CAGCGCGTCG CGATCGGCCG CGCGATCGTG CGCGAGCCCG GCGTGTTCCT GTTCGACGAA CCGCTGTCCA ATCTCGACGC GGCGCTGCGC GGCCAGACCC GCATCGAGAT CGCGCGGCTG CACCGGCAGT TCGAACGCGC GAGCGTCGTC TACGTGACGC ACGACCAGAC CGAGGCGATG ACGCTCGCCG ACAAGATCGT GCTGCTGCAC GCCGGCGCGG ATACCGCGCA GCACGGCAGC ATCGCGCAGG TCGGTGCACC GCTCGACCTT TACCACCACC CGGCCAGCCG CTTCGTCGCG GGCTTCATCG GCTCGCCGCG GATGAACTTC CTGCCGGCCG TCGTGACTGC GTGCGACGCG CAGCGCACGA CCGTCAAGCT GGTGCCGTCC GGCGAAACCT TCGCGCTGCC GCGTGACGGC TCGGCGCTGA GCGCCGGCGC CGCCGTGACG CTCGGCGTCC GTCCCGAACA CCTGACGCTC GGCGCCGCGG CCGACGCGAC GACGCTCGCG CGCGACGTCG CGCTCGTCGA ACGCCTGGGC GAACAGACCT ACGTGCACCT CGATTCGCCC GGCCTGCCCG ACGCGACGCC GCTGATCGCG AAGGTGCCCG GCGACGCGCA GGTGCGCACC GGCGAACGCG TGCACGTGCA CGCGCCGGCC GCGGCCTGCC ACCTGTTCAC CGAAGACGGC CGCGCGGTGC CCGCGTGCGC CGACGTCCAC GTTCACCACT ACGCA
Details for BuceA.00057.t.A1.GE33183
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASISMRRV QKAYGEHAPV IRDVDLEIGA HEFCVFLGPS GCGKSTLLRM IAGLEDLSAG ELSIDGRRVD DVPAAERGVA MVFQSYALFP HMTVYENMAF GLKLARVPKA EIDRKVRDAA RILQLDTLLE RKPKALSGGQ RQRVAIGRAI VREPGVFLFD EPLSNLDAAL RGQTRIEIAR LHRQFERASV VYVTHDQTEA MTLADKIVLL HAGADTAQHG SIAQVGAPLD LYHHPASRFV AGFIGSPRMN FLPAVVTACD AQRTTVKLVP SGETFALPRD GSALXAGAAV TLGVRPEHLT LGAAXRXDDA XXRRXARXXX GXQXYXXXXS PXXXXXXXXX RXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
tttntttant ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcgagc atctcgatgc gacgcgtgca gaaagcctat ggcgagcacg cgccggtcat tcgcgacgtc gatctggaga tcggcgcgca cgagttctgc gtgtttctcg ggccgtccgg atgcgggaag tcgaccctgt tgcgcatgat cgccgggctc gaggatctga gcgcgggcga gttgtcgatc gacggccgcc gcgtcgacga cgtgccggcc gccgaacgcg gcgtcgcgat ggtgttccag agctacgcgc tgtttccgca catgacggtg tacgagaaca tggcgttcgg gctgaagctc gcgcgcgtgc cgaaagccga gatcgaccgg aaggtgcgcg acgccgcgcg catcctgcag ctcgacacgc tgctcgaacg caagccgaag gcgctgtcgg gcggccagcg gcagcgcgtc gcgatcggcc gcgcgatcgt gcgcgagccc ggcgtgttcc tgttcgacga accgctgtcc aatctcgacg cggcgctgcg cggccagacc cgcatcgaga tcgcgcggct gcaccggcag ttcgaacgcg cgagcgtcgt ctacgtgacg cacgaccaga ccgaggcgat gacgctcgcc gacaagatcg tgctgctgca cgccggcgcg gataccgcgc agcacggcag catcgcgcag gtcggtgcac cgctcgacct ttaccaccac ccggccagcc gcttcgtcgc gggcttcatc ggctcgccgc ggatgaactt cctgccggcc gtcgtgactg cgtgcgacgc gcagcgcacg accgtcaagc tggtgccgtc cggcgaaacc ttcgcgctgc cgcgtgacgg ctcggcgctg ancgccggcg ccgccgtgac gctcggcgtc cgtcccgaac acctgacgct cggcgccgcn gnccgangcg acgacgctng ngngcgacgt cncgctcgtc nangnntggg nnancagann tacnnncann ncnattcncc cngangcnnc nnnnnnntcg nnnnnnnccg nnannnncnn nnnnncnncn nnnangnnnn nnnnnnnnnn cnnnngcngn gnnnngnnnn nnnnnnnnnn nnnnnnnnnn a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASISMRRV QKAYGEHAPV IRDVDLEIGA HEFCVFLGPS GCGKSTLLRM IAGLEDLSAG ELSIDGRRVD DVPAAERGVA MVFQSYALFP HMTVYENMAF GLKLARVPKA EIDRKVRDAA RILQLDTLLE RKPKALSGGQ RQRVAIGRAI VREPGVFLFD EPLSNLDAAL RGQTRIEIAR LHRQFERASV VYVTHDQTEA MTLADKIVLL HAGADTAQHG SIAQVGAPLD LYHHPASRFV AGFIGSPRMN FLPAVVTACD AQRTTVKLVP SGETFALPRD GSALSAGAAV TLGVRPEHLT LGAAADATTL ARDVALVERL GEQTYVHLDS PGLPDATPLI AKVPGDAQVR TGERVHVHAP AAACHLFTED GRAVPACADV HVHHYA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgagcatc tcgatgcgac gcgtgcagaa agcctatggc gagcacgcgc cggtcattcg cgacgtcgat ctggagatcg gcgcgcacga gttctgcgtg tttctcgggc cgtccggatg cgggaagtcg accctgttgc gcatgatcgc cgggctcgag gatctgagcg cgggcgagtt gtcgatcgac ggccgccgcg tcgacgacgt gccggccgcc gaacgcggcg tcgcgatggt gttccagagc tacgcgctgt ttccgcacat gacggtgtac gagaacatgg cgttcgggct gaagctcgcg cgcgtgccga aagccgagat cgaccggaag gtgcgcgacg ccgcgcgcat cctgcagctc gacacgctgc tcgaacgcaa gccgaaggcg ctgtcgggcg gccagcggca gcgcgtcgcg atcggccgcg cgatcgtgcg cgagcccggc gtgttcctgt tcgacgaacc gctgtccaat ctcgacgcgg cgctgcgcgg ccagacccgc atcgagatcg cgcggctgca ccggcagttc gaacgcgcga gcgtcgtcta cgtgacgcac gaccagaccg aggcgatgac gctcgccgac aagatcgtgc tgctgcacgc cggcgcggat accgcgcagc acggcagcat cgcgcaggtc ggtgcaccgc tcgaccttta ccaccacccg gccagccgct tcgtcgcggg cttcatcggc tcgccgcgga tgaacttcct gccggccgtc gtgactgcgt gcgacgcgca gcgcacgacc gtcaagctgg tgccgtccgg cgaaaccttc gcgctgccgc gtgacggctc ggcgctgagc gccggcgccg ccgtgacgct cggcgtccgt cccgaacacc tgacgctcgg cgccgcggcc gacgcgacga cgctcgcgcg cgacgtcgcg ctcgtcgaac gcctgggcga acagacctac gtgcacctcg attcgcccgg cctgcccgac gcgacgccgc tgatcgcgaa ggtgcccggc gacgcgcagg tgcgcaccgg cgaacgcgtg cacgtgcacg cgccggccgc ggcctgccac ctgttcaccg aagacggccg cgcggtgccc gcgtgcgccg acgtccacgt tcaccactac gcataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa