BuceA.00057.s

ABC transporter ATP-binding protein

CENTER ID: BuceA.00057.s
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00057.s.A1.GE33182 Full length( BuceA.00057.s ) 1 243
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4307
RefSeq: YP_002233237.1
UniProt: B4EL34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDALLKLEH LDTFYGPVQV HFDVNFEVGR GQIVSLLGGN ASGKSTTMKL ILGLMRPKRG VVRFDGDDVT ALATPQRVRR GIAAVPEARR LFADMSVREN LLMGAYTRGD RAAVADDYER VLDLFPRVRE RLAQRAGTLS GGEQQMLAMA RALMARPKLI CMDEPTMGLS PLYVDKVLEL IDAINRQGVT VFMVEQNASL ALEIAHYGYV LQTGRVVLEG PAKTLLDDER VRDAYLGGEA VAV
NT Sequence
ATGACCGATG CGCTGCTGAA ACTCGAACAT CTCGACACGT TCTACGGACC GGTGCAGGTG CACTTCGACG TGAACTTCGA GGTCGGTCGC GGGCAGATCG TCAGTCTGCT CGGCGGCAAC GCGAGCGGCA AGTCGACGAC GATGAAGCTG ATTCTCGGGC TGATGCGGCC GAAGCGCGGC GTCGTGCGTT TCGACGGCGA CGACGTGACG GCGCTCGCGA CGCCGCAGCG CGTGCGGCGC GGGATCGCGG CCGTGCCGGA GGCGCGGCGG CTGTTCGCCG ACATGAGCGT GCGCGAGAAC CTGCTGATGG GTGCATACAC GCGCGGCGAT CGCGCGGCGG TGGCCGACGA CTACGAGCGT GTGCTCGACC TGTTTCCGCG GGTGCGCGAA CGGCTTGCGC AGCGTGCGGG CACGCTGTCG GGCGGCGAGC AGCAGATGCT CGCGATGGCG CGCGCATTGA TGGCGCGACC GAAGCTGATC TGCATGGACG AGCCGACGAT GGGACTGTCG CCGCTCTACG TCGACAAGGT GCTCGAACTG ATCGACGCGA TCAACCGGCA AGGCGTGACG GTGTTCATGG TCGAGCAGAA CGCGAGCCTC GCGCTGGAGA TCGCGCACTA CGGGTACGTG CTGCAGACGG GGCGCGTCGT GCTGGAAGGG CCCGCGAAAA CGCTGCTCGA CGACGAGCGC GTGCGCGATG CGTATCTCGG TGGGGAGGCG GTGGCGGTG
Details for BuceA.00057.s.A1.GE33182
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 67
Validated AA Sequence
MTDALLKLEH LDTFYGPVQV HFDVNFEVGR GQIVSLLGGN ASGKSTTMKL ILGLMRPKRG VVRFDGDDVT ALATPQRVRR GIAAVPEARR LFADMSVREN LLMGAYTRGD RAAVADDYER VLDLFPRVRE RLAQRAGTLS GGEQXMLAMA RALMARPKLI CMDEPTMGLS PLYVDRCSN
Validated NT Sequence
gnnnnncctn ggaagctcng acccagggtc cnggttcgat gaccgatgcg ctgctgaaac tcgaacatct cgacacgttc tacggaccgg tgcaggtgca cttcgacgtg aacttcgagg tcggtcgcgg gcagatcgtc agtctgctcg gcggcaacgc gagcggcaag tcgacgacga tgaagctgat tctcgggctg atgcggccga agcgcggcgt cgtgcgtttc gacggcgacg acgtgacggc gctcgcgacg ccgcagcgcg tgcggcgcgg gatcgcggcc gtgccggagg cgcggcggct gttcgccgac atgagcgtgc gcgagaacct gctgatgggt gcatacacgc gcggcgatcg cgcggcggtg gccgacgact acgagcgtgt gctcgacctg tttccgcggg tgcgcgaacg gcttgcgcag cgtgcgggca cgctgtcggg cggcgagcag canatgctcg cgatggcgcg cgcattgatg gcgcgaccga agctgatctg catggacgag ccgacgatgg gactgtcgcc gctctacgtc gacaggtgct cgaactgatc gacgcgatca accggcaagg cgtgacggtg ttcatggtcn agcagaacgc gagcctcgcg ctggagatcg cgcactacgg gtacntgctg cagacngggc gcgtcgtgct ggangggccc gcgaaaacgc tgctcgacga cnagcgcgtg cgcnntgcgt atctcgntgg ggaggcggtg gcggtgtaat aaacagcacg aacaagttct gcancccaag cttctcgagg natccnggct gctaacnaag cccgaaaagn agctganttn gctgctgncc accgctgann naannnnnnn nnncna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDALLKLE HLDTFYGPVQ VHFDVNFEVG RGQIVSLLGG NASGKSTTMK LILGLMRPKR GVVRFDGDDV TALATPQRVR RGIAAVPEAR RLFADMSVRE NLLMGAYTRG DRAAVADDYE RVLDLFPRVR ERLAQRAGTL SGGEQQMLAM ARALMARPKL ICMDEPTMGL SPLYVDKVLE LIDAINRQGV TVFMVEQNAS LALEIAHYGY VLQTGRVVLE GPAKTLLDDE RVRDAYLGGE AVAV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgatgcg ctgctgaaac tcgaacatct cgacacgttc tacggaccgg tgcaggtgca cttcgacgtg aacttcgagg tcggtcgcgg gcagatcgtc agtctgctcg gcggcaacgc gagcggcaag tcgacgacga tgaagctgat tctcgggctg atgcggccga agcgcggcgt cgtgcgtttc gacggcgacg acgtgacggc gctcgcgacg ccgcagcgcg tgcggcgcgg gatcgcggcc gtgccggagg cgcggcggct gttcgccgac atgagcgtgc gcgagaacct gctgatgggt gcatacacgc gcggcgatcg cgcggcggtg gccgacgact acgagcgtgt gctcgacctg tttccgcggg tgcgcgaacg gcttgcgcag cgtgcgggca cgctgtcggg cggcgagcag cagatgctcg cgatggcgcg cgcattgatg gcgcgaccga agctgatctg catggacgag ccgacgatgg gactgtcgcc gctctacgtc gacaaggtgc tcgaactgat cgacgcgatc aaccggcaag gcgtgacggt gttcatggtc gagcagaacg cgagcctcgc gctggagatc gcgcactacg ggtacgtgct gcagacgggg cgcgtcgtgc tggaagggcc cgcgaaaacg ctgctcgacg acgagcgcgt gcgcgatgcg tatctcggtg gggaggcggt ggcggtgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa