SSGCID: Target Page

BuceA.00057.o

ABC transporter ATP-binding protein

CENTER ID:	BuceA.00057.o
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00057.o.A1.GE33178	Full length( BuceA.00057.o )		1	244

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.3944
RefSeq:	YP_002232892.1
UniProt:	B4EI73

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNAPIIKGVG LYRAFGGINA IAGVDVEIGA RDLLCVIGPN GAGKSTLVGL MSGAIVPGAG ELYLAGERMT GRPMYQYCRR GVVRKFQGTN VFQTMSVREN LEVAALGVSN RQAFSMRDLD GVLEQIGLAA QADMLAMHLP HGERQWLEIG MAMMCRPALL LLDEPAAGLS ATDAQRLVHL IHRLREQCAV LAIEHDLHFV QSLDCETWVM HRGQIVRQGR YDDIAKDEEV RRIYLGQGAS HAAH

NT Sequence

ATGAACGCGC CGATCATCAA AGGTGTTGGA CTGTACCGTG CATTCGGTGG CATTAACGCG ATCGCGGGCG TTGACGTAGA AATTGGCGCG AGAGACTTGC TTTGCGTCAT CGGCCCGAAC GGCGCTGGAA AAAGCACGCT GGTTGGGCTG ATGTCGGGCG CGATTGTGCC TGGGGCCGGC GAGCTGTATC TGGCGGGCGA GCGAATGACT GGTCGACCGA TGTATCAATA CTGCAGGCGT GGCGTCGTAC GGAAATTCCA GGGAACGAAT GTCTTCCAGA CGATGAGCGT ACGCGAGAAC CTCGAAGTGG CCGCGCTCGG TGTGTCGAAT CGACAGGCGT TTTCGATGCG GGACCTCGAT GGCGTTCTCG AGCAAATCGG CCTTGCCGCG CAAGCCGACA TGCTGGCGAT GCATCTGCCA CACGGCGAGC GCCAGTGGTT GGAAATCGGC ATGGCGATGA TGTGTCGCCC CGCATTGTTG CTGCTCGACG AGCCCGCTGC GGGTTTATCC GCCACTGATG CTCAGCGGCT TGTGCATCTC ATCCACCGCC TGCGTGAGCA GTGTGCGGTA CTTGCCATCG AACATGACCT GCATTTCGTG CAGTCGCTTG ATTGCGAGAC GTGGGTTATG CACCGCGGTC AAATCGTTCG CCAAGGGCGC TACGACGACA TCGCGAAGGA CGAGGAAGTC CGACGCATTT ATCTGGGGCA GGGGGCAAGT CATGCTGCAC AT

Details for BuceA.00057.o.A1.GE33178

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNAPIIKGV GLYRAFGGIN AIAGVDVEIG ARDLLCVIGP NGAGKSTLVG LMSGAIVPGA GELYLAGERM TGRPMYQYCR RGVVRKFQGT NVFQTMSVRE NLEVAALGVS NRQAFSMRDL DGVLEQIGLA AQADMLAMHL PHGERQWLEI GMAMMCRPAL LLLDEPAAGL SATDAQRLVH LIHRLREQCA VLAIEHDLHF VQSLDCETWV MHRGQIVRQG RYDDXAXDEE VRRIYLGQGA SHAAH

Validated NT Sequence

aaggannnnt accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga acgcgccgat catcaaaggt gttggactgt accgtgcatt cggtggcatt aacgcgatcg cgggcgttga cgtagaaatt ggcgcgagag acttgctttg cgtcatcggc ccgaacggcg ctggaaaaag cacgctggtt gggctgatgt cgggcgcgat tgtgcctggg gccggcgagc tgtatctggc gggcgagcga atgactggtc gaccgatgta tcaatactgc aggcgtggcg tcgtacggaa attccaggga acgaatgtct tccagacgat gagcgtacgc gagaacctcg aagtggccgc gctcggtgtg tcgaatcgac aggcgttttc gatgcgggac ctcgatggcg ttctcgagca aatcggcctt gccgcgcaag ccgacatgct ggcgatgcat ctgccacacg gcgagcgcca gtggttggaa atcggcatgg cgatgatgtg tcgccccgca ttgttgctgc tcgacgagcc cgctgcgggt ttatccgcca ctgatgctca gcggcttgtg catctcatcc accgcctgcg tgagcagtgt gcggtacttg ccatcgaaca tgacctgcat ttcgtgcagt cgcttgattg cgagacgtgg gttatgcacc gcggtcaaat cgttcgccaa gggcgctacg acgacntcgc gaangacgag gaagtccgac gcatttatct ggggcagggg gcaagtcatg ctgcacatta ataaacagca cgaacaagtt ctgcagccan gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgcnnn nnnnnnnnnn nnnnaaaaaa anaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgcgccg atcatcaaag gtgttggact gtaccgtgca ttcggtggca ttaacgcgat cgcgggcgtt gacgtagaaa ttggcgcgag agacttgctt tgcgtcatcg gcccgaacgg cgctggaaaa agcacgctgg ttgggctgat gtcgggcgcg attgtgcctg gggccggcga gctgtatctg gcgggcgagc gaatgactgg tcgaccgatg tatcaatact gcaggcgtgg cgtcgtacgg aaattccagg gaacgaatgt cttccagacg atgagcgtac gcgagaacct cgaagtggcc gcgctcggtg tgtcgaatcg acaggcgttt tcgatgcggg acctcgatgg cgttctcgag caaatcggcc ttgccgcgca agccgacatg ctggcgatgc atctgccaca cggcgagcgc cagtggttgg aaatcggcat ggcgatgatg tgtcgccccg cattgttgct gctcgacgag cccgctgcgg gtttatccgc cactgatgct cagcggcttg tgcatctcat ccaccgcctg cgtgagcagt gtgcggtact tgccatcgaa catgacctgc atttcgtgca gtcgcttgat tgcgagacgt gggttatgca ccgcggtcaa atcgttcgcc aagggcgcta cgacgacatc gcgaaggacg aggaagtccg acgcatttat ctggggcagg gggcaagtca tgctgcacat taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa