SSGCID: Target Page

BuceA.00057.n

Putative amino acid ABC transporter ATP-binding protein

CENTER ID:	BuceA.00057.n
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00057.n.A1.GE33177	Full length( BuceA.00057.n )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.6035
RefSeq:	YP_002234846.1
UniProt:	B4EGH9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSANAELLKV AGLQMRFGGL LAVDGIDFDV RRDEVFAIIG PNGAGKTTVF NCVGGFYKPT GGDVVLDGHA IGGLPSHKIA LKGLVRTFQN IRLFKSLTVV ENLLVAQHRK VKAGLLHGLF ATPAYRHAEQ AALERAAVWL DRMGLTSVAN RPAGTLSYGH QRRLEIARCM ITEPRLLMLD EPAAGLNPQE KIELQHLIDK LRREFGVSVL LIEHDMSLVM GVSDRILVME HGRPIVIGTP EAVRNDPRVI KAYLGEE

NT Sequence

ATGAGCGCGA ATGCAGAACT GTTGAAGGTG GCCGGGCTGC AGATGCGCTT CGGCGGGTTG CTCGCGGTGG ACGGCATCGA TTTCGACGTG CGCCGCGACG AGGTGTTCGC GATCATCGGG CCGAACGGCG CGGGCAAGAC CACGGTGTTC AACTGCGTCG GCGGCTTCTA CAAGCCGACC GGCGGCGACG TGGTGCTCGA CGGCCACGCG ATTGGCGGGC TGCCGAGCCA CAAGATCGCG TTGAAGGGGC TGGTGCGGAC GTTCCAGAAC ATCCGGCTGT TCAAGTCGCT GACCGTCGTC GAGAACCTGC TCGTCGCGCA GCATCGCAAG GTGAAGGCCG GGCTGCTGCA CGGCCTGTTC GCGACGCCCG CGTATCGTCA TGCCGAGCAG GCAGCGCTCG AACGCGCGGC CGTCTGGCTC GACCGGATGG GGCTGACGTC GGTCGCCAAC CGGCCGGCCG GCACGCTGTC GTACGGGCAC CAGCGGCGTC TGGAGATCGC GCGCTGCATG ATCACCGAGC CGCGCCTGCT GATGCTCGAC GAGCCGGCGG CGGGCCTCAA CCCGCAGGAA AAGATCGAGC TGCAGCACCT GATCGACAAG CTGCGCCGTG AATTCGGCGT GTCGGTGCTG CTGATCGAAC ACGACATGAG CCTCGTGATG GGCGTGTCCG ACCGCATCCT CGTGATGGAG CACGGCCGGC CGATCGTGAT CGGCACGCCG GAAGCGGTCC GCAACGACCC GCGCGTGATC AAGGCGTATC TGGGGGAAGA G

Details for BuceA.00057.n.A1.GE33177

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMSANAEL LKVAGLQMRF GGLLAVDGID FDVRRDEVFA IIGPNGAGKT TVFNCVGGFY KPTGGDVVLD GHAIGGLPSH KIALKGLVRT FQNIRLFKSL TVVENLLVAQ HRKVKAGLLH GLFATPAYRH AEQAALERAA VWLDRMGLTS VANRPAGTLS YGHQRRLEIA RCMITEPRLL MLDEPAAGLN PQEKIELQHL IDKLRREFGV SVLLIEHDMS LVMGVSDRIL VMEHGRPIVI GTPXAVRNDP RVIKAYLGXE

Validated NT Sequence

gnnnnntacc anggntcatc accatnacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcg cgaatgcaga actgttgaag gtggccgggc tgcagatgcg cttcggcggg ttgctcgcgg tggacggcat cgatttcgac gtgcgccgcg acgaggtgtt cgcgatcatc gggccgaacg gcgcgggcaa gaccacggtg ttcaactgcg tcggcggctt ctacaagccg accggcggcg acgtggtgct cgacggccac gcgattggcg ggctgccgag ccacaagatc gcgttgaagg ggctggtgcg gacgttccag aacatccggc tgttcaagtc gctgaccgtc gtcgagaacc tgctcgtcgc gcagcatcgc aaggtgaagg ccgggctgct gcacggcctg ttcgcgacgc ccgcgtatcg tcatgccgag caggcagcgc tcgaacgcgc ggccgtctgg ctcgaccgga tggggctgac gtcggtcgcc aaccggccgg ccggcacgct gtcgtacggg caccagcggc gtctggagat cgcgcgctgc atgatcaccg agccgcgcct gctgatgctc gacgagccgg cggcgggcct caacccgcag gaaaagatcg agctgcagca cctgatcgac aagctgcgcc gtgaattcgg cgtgtcggtg ctgctgatcg aacacgacat gagcctcgtg atgggcgtgt ccgaccgcat cctcgtgatg gagcacggcc ggccgatcgt gatcggcacg ccngangcgg tccgcaacga cccgcgcgtg atcaaggcgt atctggggna agagtaataa acagcacgaa caagttctgc agccnagctt ctcgaggatc cngctgctaa caaannccga aaggaagctg anttggctgc tgccaccgct nannnnnnnn nntnnnnnnn na

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSANAELLK VAGLQMRFGG LLAVDGIDFD VRRDEVFAII GPNGAGKTTV FNCVGGFYKP TGGDVVLDGH AIGGLPSHKI ALKGLVRTFQ NIRLFKSLTV VENLLVAQHR KVKAGLLHGL FATPAYRHAE QAALERAAVW LDRMGLTSVA NRPAGTLSYG HQRRLEIARC MITEPRLLML DEPAAGLNPQ EKIELQHLID KLRREFGVSV LLIEHDMSLV MGVSDRILVM EHGRPIVIGT PEAVRNDPRV IKAYLGEE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcgaat gcagaactgt tgaaggtggc cgggctgcag atgcgcttcg gcgggttgct cgcggtggac ggcatcgatt tcgacgtgcg ccgcgacgag gtgttcgcga tcatcgggcc gaacggcgcg ggcaagacca cggtgttcaa ctgcgtcggc ggcttctaca agccgaccgg cggcgacgtg gtgctcgacg gccacgcgat tggcgggctg ccgagccaca agatcgcgtt gaaggggctg gtgcggacgt tccagaacat ccggctgttc aagtcgctga ccgtcgtcga gaacctgctc gtcgcgcagc atcgcaaggt gaaggccggg ctgctgcacg gcctgttcgc gacgcccgcg tatcgtcatg ccgagcaggc agcgctcgaa cgcgcggccg tctggctcga ccggatgggg ctgacgtcgg tcgccaaccg gccggccggc acgctgtcgt acgggcacca gcggcgtctg gagatcgcgc gctgcatgat caccgagccg cgcctgctga tgctcgacga gccggcggcg ggcctcaacc cgcaggaaaa gatcgagctg cagcacctga tcgacaagct gcgccgtgaa ttcggcgtgt cggtgctgct gatcgaacac gacatgagcc tcgtgatggg cgtgtccgac cgcatcctcg tgatggagca cggccggccg atcgtgatcg gcacgccgga agcggtccgc aacgacccgc gcgtgatcaa ggcgtatctg ggggaagagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa