BuceA.00057.m

Putative amino acid ABC transporter ATP-binding protein

CENTER ID: BuceA.00057.m
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00057.m.A1.GE33176 Full length( BuceA.00057.m ) 1 233
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6034
RefSeq: YP_002234845.1
UniProt: B4EGH8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKLEQVHTH YGAVEALAGV SIEVNKGEIV TLIGSNGAGK TTLMMTVCGT PRASSGRVLF EGNDITAMST HQIMRQGMAI SPEGRRVFPS LTVLENLKMG GFFASRHEID DGIEHVFKLF PRLKERAAQR AGTMSGGEQQ MLAIGRALMS KPRLLLLDEP TLGLAPLVIA QIFDIIRTIR DEGVTVFLVE QNANKALGVA DRGYVLETGR VVLADTGANL LANDRIKQAY LGG
NT Sequence
ATGCTGAAGC TGGAACAGGT CCATACGCAC TACGGCGCGG TCGAAGCGCT CGCGGGCGTG TCGATCGAAG TGAACAAGGG CGAGATCGTC ACGCTGATCG GCAGCAACGG CGCCGGCAAG ACGACGCTGA TGATGACCGT GTGCGGCACG CCGCGCGCGT CGTCGGGCCG CGTGCTGTTC GAGGGCAACG ACATCACCGC GATGTCGACG CACCAGATCA TGCGGCAGGG AATGGCGATT TCTCCGGAAG GGCGGCGCGT GTTCCCGAGC CTGACGGTGC TCGAGAACCT GAAGATGGGC GGCTTCTTCG CGAGCCGTCA CGAGATCGAC GACGGCATCG AGCACGTGTT CAAGCTGTTT CCGCGCCTGA AGGAACGCGC GGCGCAGCGG GCCGGCACGA TGTCGGGCGG CGAGCAGCAG ATGCTGGCGA TCGGCCGCGC GCTGATGAGC AAGCCGCGTT TGCTGCTGCT CGACGAGCCG ACGCTCGGCC TCGCGCCGCT CGTGATCGCG CAGATCTTCG ACATCATCCG CACGATCCGC GACGAAGGCG TCACGGTGTT CCTCGTCGAG CAGAATGCGA ACAAGGCGCT CGGTGTGGCC GACCGCGGCT ACGTGCTCGA AACGGGGCGC GTGGTGCTGG CCGACACGGG CGCGAACCTG CTCGCGAACG ACCGCATCAA GCAGGCGTAT CTCGGCGGT
Details for BuceA.00057.m.A1.GE33176
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLKLEQVHT HYGAVEALAG VSIEVNKGEI VTLIGSNGAG KTTLMMTVCG TPRASSGRVL FEGNDITAMS THQIMRQGMA ISPEGRRVFP SLTVLENLKM GGFFASRHEI DDGIEHVFKL FPRLKERAAQ RAGTMSGGEQ QMLAIGRALM SKPRLLLLDE PTLGLAPLVI AQIFDIIRTI RDEXVTVFLV EQNANKALGV ADRGYVLETG RVVLADTGAN LLANDRIKXA YLGG
Validated NT Sequence
angagatnta ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgct gaagctggaa caggtccata cgcactacgg cgcggtcgaa gcgctcgcgg gcgtgtcgat cgaagtgaac aagggcgaga tcgtcacgct gatcggcagc aacggcgccg gcaagacgac gctgatgatg accgtgtgcg gcacgccgcg cgcgtcgtcg ggccgcgtgc tgttcgaggg caacgacatc accgcgatgt cgacgcacca gatcatgcgg cagggaatgg cgatttctcc ggaagggcgg cgcgtgttcc cgagcctgac ggtgctcgag aacctgaaga tgggcggctt cttcgcgagc cgtcacgaga tcgacgacgg catcgagcac gtgttcaagc tgtttccgcg cctgaaggaa cgcgcggcgc agcgggccgg cacgatgtcg ggcggcgagc agcagatgct ggcgatcggc cgcgcgctga tgagcaagcc gcgtttgctg ctgctcgacg agccgacgct cggcctcgcg ccgctcgtga tcgcgcagat cttcgacatc atccgcacga tccgcgacga angcgtcacg gtgttcctcg tcgagcagaa tgcgaacaag gcgctcggtg tggccgaccg cggctacgtg ctcgaaacgg ggcgcgtggt gctggccgac acgggcgcga acctgctcgc gaacgaccgc atcaagcang cgtatctcgg cggttaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cngctgctaa caaagcccga aaggaagctg agttggctgc tgnnnccgnt nnnnnnnnna nnnnaaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLKLEQVHT HYGAVEALAG VSIEVNKGEI VTLIGSNGAG KTTLMMTVCG TPRASSGRVL FEGNDITAMS THQIMRQGMA ISPEGRRVFP SLTVLENLKM GGFFASRHEI DDGIEHVFKL FPRLKERAAQ RAGTMSGGEQ QMLAIGRALM SKPRLLLLDE PTLGLAPLVI AQIFDIIRTI RDEGVTVFLV EQNANKALGV ADRGYVLETG RVVLADTGAN LLANDRIKQA YLGG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaagctg gaacaggtcc atacgcacta cggcgcggtc gaagcgctcg cgggcgtgtc gatcgaagtg aacaagggcg agatcgtcac gctgatcggc agcaacggcg ccggcaagac gacgctgatg atgaccgtgt gcggcacgcc gcgcgcgtcg tcgggccgcg tgctgttcga gggcaacgac atcaccgcga tgtcgacgca ccagatcatg cggcagggaa tggcgatttc tccggaaggg cggcgcgtgt tcccgagcct gacggtgctc gagaacctga agatgggcgg cttcttcgcg agccgtcacg agatcgacga cggcatcgag cacgtgttca agctgtttcc gcgcctgaag gaacgcgcgg cgcagcgggc cggcacgatg tcgggcggcg agcagcagat gctggcgatc ggccgcgcgc tgatgagcaa gccgcgtttg ctgctgctcg acgagccgac gctcggcctc gcgccgctcg tgatcgcgca gatcttcgac atcatccgca cgatccgcga cgaaggcgtc acggtgttcc tcgtcgagca gaatgcgaac aaggcgctcg gtgtggccga ccgcggctac gtgctcgaaa cggggcgcgt ggtgctggcc gacacgggcg cgaacctgct cgcgaacgac cgcatcaagc aggcgtatct cggcggttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa