BuceA.00057.k

ABC transporter ATP-binding protein

CENTER ID: BuceA.00057.k
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00057.k.A1.GE33174 Full length( BuceA.00057.k ) 1 284
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3217
RefSeq: YP_002232212.1
UniProt: B4ECC2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNGTALYQFT PPPEDELTVS GSASMGRVVP PGIDTSHGTI LYLEDIEVSF DGFRALKKLS LSIDAGELRC VIGPNGAGKT TMMDVITGKT RPDAGKVFLG QTIDLARMNE PEIARAGIGR KFQKPTVFEQ HPVWENLELA MQTDKGWLAS LRARLDRTAQ AKIEETLALI GLESHAYRAA GELSHGQKQR LEIGMLLMQR PALLLLDEPA AGMTDHETME LAELLNTLRG TCSMMVVEHD MEFVAALAGD TGRVTVMAEG AVLAHGTLDQ VKRDDAVIES YLGR
NT Sequence
ATGAATGGAA CGGCGCTCTA CCAGTTCACG CCGCCCCCCG AGGACGAGCT GACCGTCAGC GGCAGCGCGT CGATGGGGCG CGTCGTGCCG CCCGGCATCG ATACGTCGCA CGGCACGATT CTCTATCTCG AGGACATCGA GGTGAGCTTC GACGGGTTTC GCGCGCTGAA GAAGCTGTCG CTCTCGATCG ACGCGGGCGA GCTGCGCTGC GTGATCGGCC CGAACGGCGC GGGCAAGACG ACGATGATGG ACGTCATCAC CGGCAAGACG CGGCCCGATG CGGGCAAGGT GTTTCTGGGC CAGACGATCG ACCTCGCGCG GATGAACGAA CCCGAGATCG CGCGGGCGGG CATCGGCCGC AAGTTCCAGA AGCCGACCGT GTTCGAACAG CATCCGGTAT GGGAAAACCT CGAACTCGCG ATGCAGACCG ACAAGGGCTG GCTCGCGTCG CTGCGCGCGC GGCTCGACCG CACCGCGCAG GCGAAGATCG AGGAGACGCT CGCACTGATC GGCCTCGAAA GCCATGCGTA TCGCGCGGCC GGCGAACTGT CGCACGGCCA GAAGCAGCGG CTCGAAATCG GCATGCTGCT GATGCAGCGC CCCGCGCTGC TGCTGCTCGA CGAGCCGGCG GCCGGGATGA CCGACCACGA GACGATGGAA CTCGCCGAGC TGCTGAACAC GCTGCGCGGC ACCTGCTCGA TGATGGTCGT CGAGCACGAC ATGGAATTCG TCGCGGCGCT CGCGGGCGAC ACGGGACGCG TGACGGTGAT GGCCGAAGGC GCGGTGCTCG CGCACGGCAC GCTCGACCAG GTCAAGCGCG ACGACGCGGT GATCGAGTCT TACCTCGGAC GA
Details for BuceA.00057.k.A1.GE33174
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 33kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNGTALYQF TPPPEDELTV SGSASMGRVV PPGIDTSHGT ILYLEDIEVS FDGFRALKKL SLSIDAGELR CVIGPNGAGK TTMMDVITGK TRPDAGKVFL GQTIDLARMN EPEIARAGIG RKFQKPTVFE QHPVWENLEL AMQTDKGWLA SLRARLDRTA QAKIEETLAL IGLESHAYRA AGELSHGQKQ RLEIGMLLMQ RPALLLLDEP AAGMTDHETM ELAELLNTLR GTCSMMVVEH DMEFVAALAG DTGRVTVMAE XAVLAHGTLD QVKRDDXVIE SYLXRX
Validated NT Sequence
atttnnntta ntttaagaag gaganntacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaatg gaacggcgct ctaccagttc acgccgcccc ccgaggacga gctgaccgtc agcggcagcg cgtcgatggg gcgcgtcgtg ccgcccggca tcgatacgtc gcacggcacg attctctatc tcgaggacat cgaggtgagc ttcgacgggt ttcgcgcgct gaagaagctg tcgctctcga tcgacgcggg cgagctgcgc tgcgtgatcg gcccgaacgg cgcgggcaag acgacgatga tggacgtcat caccggcaag acgcggcccg atgcgggcaa ggtgtttctg ggccagacga tcgacctcgc gcggatgaac gaacccgaga tcgcgcgggc gggcatcggc cgcaagttcc agaagccgac cgtgttcgaa cagcatccgg tatgggaaaa cctcgaactc gcgatgcaga ccgacaaggg ctggctcgcg tcgctgcgcg cgcggctcga ccgcaccgcg caggcgaaga tcgaggagac gctcgcactg atcggcctcg aaagccatgc gtatcgcgcg gccggcgaac tgtcgcacgg ccagaagcag cggctcgaaa tcggcatgct gctgatgcag cgccccgcgc tgctgctgct cgacgagccg gcggccggga tgaccgacca cgagacgatg gaactcgccg agctgctgaa cacgctgcgc ggcacctgct cgatgatggt cgtcgagcac gacatggaat tcgtcgcggc gctcgcgggc gacacgggac gcgtgacggt gatggccgaa nncgcggtgc tcgcgcacgg cacgctcgac caggtcaagc gcgacgacnc ggtgatcgag tcttacctcn gacganaata aacagcacga acaagttctg cagccnanct tctcgaggan nnnctgctna caagcccgaa annngctgan ttggctgctn cnnnnnnnaa naaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNGTALYQF TPPPEDELTV SGSASMGRVV PPGIDTSHGT ILYLEDIEVS FDGFRALKKL SLSIDAGELR CVIGPNGAGK TTMMDVITGK TRPDAGKVFL GQTIDLARMN EPEIARAGIG RKFQKPTVFE QHPVWENLEL AMQTDKGWLA SLRARLDRTA QAKIEETLAL IGLESHAYRA AGELSHGQKQ RLEIGMLLMQ RPALLLLDEP AAGMTDHETM ELAELLNTLR GTCSMMVVEH DMEFVAALAG DTGRVTVMAE GAVLAHGTLD QVKRDDAVIE SYLGR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatggaacg gcgctctacc agttcacgcc gccccccgag gacgagctga ccgtcagcgg cagcgcgtcg atggggcgcg tcgtgccgcc cggcatcgat acgtcgcacg gcacgattct ctatctcgag gacatcgagg tgagcttcga cgggtttcgc gcgctgaaga agctgtcgct ctcgatcgac gcgggcgagc tgcgctgcgt gatcggcccg aacggcgcgg gcaagacgac gatgatggac gtcatcaccg gcaagacgcg gcccgatgcg ggcaaggtgt ttctgggcca gacgatcgac ctcgcgcgga tgaacgaacc cgagatcgcg cgggcgggca tcggccgcaa gttccagaag ccgaccgtgt tcgaacagca tccggtatgg gaaaacctcg aactcgcgat gcagaccgac aagggctggc tcgcgtcgct gcgcgcgcgg ctcgaccgca ccgcgcaggc gaagatcgag gagacgctcg cactgatcgg cctcgaaagc catgcgtatc gcgcggccgg cgaactgtcg cacggccaga agcagcggct cgaaatcggc atgctgctga tgcagcgccc cgcgctgctg ctgctcgacg agccggcggc cgggatgacc gaccacgaga cgatggaact cgccgagctg ctgaacacgc tgcgcggcac ctgctcgatg atggtcgtcg agcacgacat ggaattcgtc gcggcgctcg cgggcgacac gggacgcgtg acggtgatgg ccgaaggcgc ggtgctcgcg cacggcacgc tcgaccaggt caagcgcgac gacgcggtga tcgagtctta cctcggacga taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa