SSGCID: Target Page

BuceA.00057.i

Taurine ABC transporter ATP-binding protein

CENTER ID:	BuceA.00057.i
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00057.i.A1.GE33172	Full length( BuceA.00057.i )		1	265

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.747
RefSeq:	YP_002229870.1
UniProt:	B4E9R2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTLEVRQVS VAYPGERGRP TTQALAQVDL RIDAGEFVVA LGASGCGKTT LLNCMAGFVA PTTGDVRVDG VPVTGPGADR GVVFQKYALL PWLDVLDNVA LGLRFARVSK AERDARAREM LTLVGLERHA HARVYELSGG MQQRVGIARA LASDPRVLLM DEPMGALDAM TRGTMQALVL DVWARTGKTV FFITHDVEEA LFLATRLIVM TAGPGRIAET FELPFARRYV ESRDARAVKS SPDFIAWRER LIAYLHRDEA AVEPA

NT Sequence

ATGAGCACGC TGGAAGTCCG TCAGGTATCG GTCGCCTATC CGGGCGAGCG CGGCAGGCCG ACGACGCAGG CGCTCGCGCA GGTCGACCTG CGCATCGATG CCGGTGAATT CGTCGTCGCG CTCGGCGCGT CCGGGTGCGG CAAGACCACG CTGCTGAACT GCATGGCCGG CTTCGTCGCA CCGACGACGG GCGACGTGCG GGTCGACGGC GTGCCGGTCA CGGGCCCCGG CGCCGATCGC GGCGTCGTGT TCCAGAAATA CGCGCTGCTG CCGTGGCTCG ACGTGCTCGA CAACGTCGCG CTCGGACTGC GCTTCGCGCG CGTGTCGAAG GCCGAACGCG ATGCACGGGC GCGCGAGATG CTCACACTCG TCGGCCTCGA GCGTCATGCG CATGCGCGCG TGTACGAGCT GTCGGGCGGG ATGCAGCAGC GGGTCGGCAT CGCGCGGGCG CTCGCGAGCG ACCCGCGCGT GCTGCTGATG GACGAGCCGA TGGGCGCGCT CGATGCGATG ACGCGCGGCA CGATGCAGGC GCTCGTGCTC GACGTGTGGG CGCGCACCGG CAAGACGGTG TTCTTCATCA CGCACGACGT CGAGGAGGCG TTGTTCCTCG CGACGCGTCT GATCGTGATG ACGGCGGGCC CCGGTCGCAT TGCCGAAACC TTCGAGCTGC CGTTCGCGCG CCGCTACGTC GAATCGCGCG ATGCGCGCGC GGTGAAGTCG TCGCCGGACT TCATCGCGTG GCGCGAGCGG TTGATCGCGT ACCTGCATCG CGACGAGGCG GCGGTGGAGC CCGCG

Details for BuceA.00057.i.A1.GE33172

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	97
PERCENT COVERAGE:	103

Validated AA Sequence

MGTLEAQTQG PGSMSTLEVR QVSVAYPGER GRPTTQALAQ VDLRIDAGEF VVALGASGCG KTTLLNCMAG FVAPTTGDVR VDGVPVTGPG ADRGVVFQKY ALLPWLDVLD NVALGLRFAR VSKAERDARA REMLTLVGLE RHAHARVYEL SGGMQQRVGI ARALASDPRV LLMDEPMGAL DAMTRGTMXA LVLDVWARTG KTVFFITHDV EEALFLATRL IVMTAGPGRI AETFELPFAR RYVESRDARA VKSSPDFIAW RERLIAYLHX XXXAXGGARV INSTNKFCSQ ASRGSGC

Validated NT Sequence

tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatgag cacgctggaa gtccgtcagg tatcggtcgc ctatccgggc gagcgcggca ggccgacgac gcaggcgctc gcgcaggtcg acctgcgcat cgatgccggt gaattcgtcg tcgcgctcgg cgcgtccggg tgcggcaaga ccacgctgct gaactgcatg gccggcttcg tcgcaccgac gacgggcgac gtgcgggtcg acggcgtgcc ggtcacgggc cccggcgccg atcgcggcgt cgtgttccag aaatacgcgc tgctgccgtg gctcgacgtg ctcgacaacg tcgcgctcgg actgcgcttc gcgcgcgtgt cgaaggccga acgcgatgca cgggcgcgcg agatgctcac actcgtcggc ctcgagcgtc atgcgcatgc gcgcgtgtac gagctgtcgg gcgggatgca gcagcgggtc ggcatcgcgc gggcgctcgc gagcgacccg cgcgtgctgc tgatggacga gccgatgggc gcgctcgatg cgatgacgcg cggcacgatg cangcgctcg tgctcgacgt gtgggcgcgc accggcaaga cggtgttctt catcacgcac gacgtcgagg aggcgttgtt cctcgcgacg cgtctgatcg tgatgacngc gggccccggt cgcattgccg aaaccttcga gctgccgttc gcgcgccgct acgtcgaatc gcgcgatgcg cgcgcggtga agtcgtcgcc ngacttcatc gcgtggcgcg agcggttgat cgcgtacctg catcnnngnn cnanggcggn cggtggagcc cgcgtaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg anttgnnnnn nnnnnnnnnn nnnnaannnn nnaaaaaaaa a

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTLEVRQV SVAYPGERGR PTTQALAQVD LRIDAGEFVV ALGASGCGKT TLLNCMAGFV APTTGDVRVD GVPVTGPGAD RGVVFQKYAL LPWLDVLDNV ALGLRFARVS KAERDARARE MLTLVGLERH AHARVYELSG GMQQRVGIAR ALASDPRVLL MDEPMGALDA MTRGTMQALV LDVWARTGKT VFFITHDVEE ALFLATRLIV MTAGPGRIAE TFELPFARRY VESRDARAVK SSPDFIAWRE RLIAYLHRDE AAVEPA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcacgctg gaagtccgtc aggtatcggt cgcctatccg ggcgagcgcg gcaggccgac gacgcaggcg ctcgcgcagg tcgacctgcg catcgatgcc ggtgaattcg tcgtcgcgct cggcgcgtcc gggtgcggca agaccacgct gctgaactgc atggccggct tcgtcgcacc gacgacgggc gacgtgcggg tcgacggcgt gccggtcacg ggccccggcg ccgatcgcgg cgtcgtgttc cagaaatacg cgctgctgcc gtggctcgac gtgctcgaca acgtcgcgct cggactgcgc ttcgcgcgcg tgtcgaaggc cgaacgcgat gcacgggcgc gcgagatgct cacactcgtc ggcctcgagc gtcatgcgca tgcgcgcgtg tacgagctgt cgggcgggat gcagcagcgg gtcggcatcg cgcgggcgct cgcgagcgac ccgcgcgtgc tgctgatgga cgagccgatg ggcgcgctcg atgcgatgac gcgcggcacg atgcaggcgc tcgtgctcga cgtgtgggcg cgcaccggca agacggtgtt cttcatcacg cacgacgtcg aggaggcgtt gttcctcgcg acgcgtctga tcgtgatgac ggcgggcccc ggtcgcattg ccgaaacctt cgagctgccg ttcgcgcgcc gctacgtcga atcgcgcgat gcgcgcgcgg tgaagtcgtc gccggacttc atcgcgtggc gcgagcggtt gatcgcgtac ctgcatcgcg acgaggcggc ggtggagccc gcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa