BuceA.00057.h

ABC transporter ATP-binding protein

CENTER ID: BuceA.00057.h
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00057.h.A1.GE33171 Full length( BuceA.00057.h ) 1 258
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2802
RefSeq: YP_002231810.1
UniProt: B4E8Y0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDKQIRLSV SGVNKRFGGL QALSDVGLQI REGEIYGLIG PNGAGKTTFF NVITGLYTPD SGDFKLDGTN YTPTAVHQVA KAGIARTFQN IRLFGGMTAL ENVMVGRHVR TKHGLLGAVF QTPAERQEER EIKERAIELL EYVGVLQYAD YTSRNLSYGH QRRLEIARAL ATDPKLLALD EPAAGMNATE KVELTRLLDK IRSDGRTILL IEHDVKLVMG LCNRMTVLDY GKVIAEGLPQ DVQKNPKVIE AYLGAGVH
NT Sequence
ATGAGCGACA AGCAAATCCG ACTGTCCGTC TCGGGCGTGA ACAAGCGCTT CGGCGGGCTG CAGGCACTGT CCGACGTCGG CCTGCAGATC CGCGAAGGCG AGATCTACGG TCTGATCGGC CCGAACGGGG CCGGCAAGAC GACGTTCTTC AACGTGATCA CGGGCCTCTA CACGCCCGAC TCCGGCGACT TCAAGCTGGA CGGCACGAAC TACACGCCGA CGGCCGTGCA CCAGGTGGCC AAGGCCGGCA TCGCGCGCAC GTTCCAGAAC ATCCGCCTGT TCGGCGGGAT GACGGCGCTC GAGAACGTGA TGGTGGGCCG CCACGTGCGC ACCAAGCACG GTCTGCTGGG CGCGGTGTTC CAGACGCCGG CCGAACGCCA GGAAGAGCGC GAGATCAAGG AGCGCGCGAT CGAGCTGCTC GAATACGTCG GCGTGCTGCA GTACGCGGAC TACACGTCGC GCAACCTGTC GTACGGTCAC CAGCGTCGTC TCGAAATCGC CCGCGCGCTC GCGACCGACC CGAAGCTGCT CGCGCTCGAC GAGCCGGCGG CCGGGATGAA CGCGACCGAG AAGGTCGAAC TCACGCGCCT GCTCGACAAG ATCCGCTCGG ACGGCCGCAC GATTCTCCTG ATCGAGCACG ACGTGAAGCT CGTGATGGGG TTGTGCAACC GGATGACGGT GCTCGATTAC GGCAAGGTGA TCGCCGAGGG TCTGCCGCAG GACGTGCAGA AGAATCCGAA GGTGATTGAG GCATATCTCG GCGCAGGGGT GCAC
Details for BuceA.00057.h.A1.GE33171
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 26
PERCENT COVERAGE: 94
Validated AA Sequence
MXQSXSDXSA RXRQTLGDHL AVIEHRHPVA QPHHELHVVL DQENRAAVRA DLVEQAREFD LLGRVHPGRR LVEREQLRVG RERAGDFETT LVTVRQVARR VVRVLQHADV FEQLDRALLD LALFLAFGRR LEHRAQQTVL GAHVAAHHHV LERRHPAEQA DVLERARDAG LGHLVHGRRR VVRAVQLEVA GVGRVEARDH VEERRLAGPV RADQTVDLAF ADLQADVGQC LQPAEALVHA RDGQSDLLVA HRTRTLGXSF XGXP
Validated NT Sequence
tnggnnnccc nnggaagctc ngacccaggg tcctggttcg atgagcgaca agcaaatccg actgtccgtc tcgggcgtga acaagcgctt cggcgggctg caggcactgt ccgacgtcgg cctgcagatc cgcgaaggcg agatctacgg tctgatcggc ccgaacgggg ccggcaagac gacgttcttc aacgtgatca cgggcctcta cacgcccgac tccggcgact tcaagctgga cggcacgaac tacacgccga cggccgtgca ccaggtggcc aaggccggca tcgcgcgcac gttccagaac atccgcctgt tcggcgggat gacggcgctc gagaacgtga tggtgggccg ccacgtgcgc accaagcacg gtctgctggg cgcggtgttc cagacgccgg ccgaacgcca ggaagagcgc gagatcaagg agcgcgcgat cgagctgctc gaatacgtcg gcgtgctgca gtacgcggac tacacgtcgc gcaacctgtc gtacggtcac cagcgtcgtc tcgaaatcgc ccgcgcgctc gcgaccgacc cgaagctgct cgcgctcgac gagccggcgg ccgggatgaa cgcgaccgag aaggtcgaac tcacgcgcct gctcgacaag atccgctcgg acggccgcac gattctcctg atcgagcacg acgtgaagct cgtgatgggg ttgtgcaacc ggatgacggt gctcgattac ngcaaggtga tcgccgaggg tctgccgcnn ncgtgcagaa naatccgaan gtgattgang catatctcgg cgcnnggtgc actaataaac agcacgaaca agttctgcag ccaagcttct cgaggatncc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccnccgctn nannnnnnnn nnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDKQIRLS VSGVNKRFGG LQALSDVGLQ IREGEIYGLI GPNGAGKTTF FNVITGLYTP DSGDFKLDGT NYTPTAVHQV AKAGIARTFQ NIRLFGGMTA LENVMVGRHV RTKHGLLGAV FQTPAERQEE REIKERAIEL LEYVGVLQYA DYTSRNLSYG HQRRLEIARA LATDPKLLAL DEPAAGMNAT EKVELTRLLD KIRSDGRTIL LIEHDVKLVM GLCNRMTVLD YGKVIAEGLP QDVQKNPKVI EAYLGAGVH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgacaag caaatccgac tgtccgtctc gggcgtgaac aagcgcttcg gcgggctgca ggcactgtcc gacgtcggcc tgcagatccg cgaaggcgag atctacggtc tgatcggccc gaacggggcc ggcaagacga cgttcttcaa cgtgatcacg ggcctctaca cgcccgactc cggcgacttc aagctggacg gcacgaacta cacgccgacg gccgtgcacc aggtggccaa ggccggcatc gcgcgcacgt tccagaacat ccgcctgttc ggcgggatga cggcgctcga gaacgtgatg gtgggccgcc acgtgcgcac caagcacggt ctgctgggcg cggtgttcca gacgccggcc gaacgccagg aagagcgcga gatcaaggag cgcgcgatcg agctgctcga atacgtcggc gtgctgcagt acgcggacta cacgtcgcgc aacctgtcgt acggtcacca gcgtcgtctc gaaatcgccc gcgcgctcgc gaccgacccg aagctgctcg cgctcgacga gccggcggcc gggatgaacg cgaccgagaa ggtcgaactc acgcgcctgc tcgacaagat ccgctcggac ggccgcacga ttctcctgat cgagcacgac gtgaagctcg tgatggggtt gtgcaaccgg atgacggtgc tcgattacgg caaggtgatc gccgagggtc tgccgcagga cgtgcagaag aatccgaagg tgattgaggc atatctcggc gcaggggtgc actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa